• Asian-Australasian Journal of Animal Sciences
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• 제17권2호
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• pp.168-173
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• 2004

The ability of frozen-thawed fetal skin was examined to generate viable cell lines for nuclear transfer. Fetal skin frozen at -20$-20^{\circ}C$, $-30^{\circ}C$ or $-80^{\circ}C$ in the presence of 5% DMSO used as tissue explants to generate somatic cells. The resultant confluent cells were then used as donors for nuclear transfer (NT). Of the bovine NT embryos reconstracted from the somatic cells, 62.3%, 76.6% to 65% showed cleavage 70.5%, 81.9% to 78.5% reached the stage of morula formation and 39.7%, 43.2% or 47.6% reached the blastocyst stage. There was no significant difference in development when the NT embryos were compared with those reconstracted from fresh somatic cell derieved skin tissues (72%, 75.3%, and 45.2%, for cleavage, and development to morula and blastocyst stage, respectively). NT embryos were then placed in a portable $CO_2$ incubator and carried to China from Japan by air. After reaching to farm, two NT embryos were transferred to each of 5 recipients. We obtained 2 NT calves which birth weights is 30kg and 36kg female, and gestation periods is 281 and 284 days, respectively. There were no observation any abnormality from those calves. The results indicated that cell lines derieved from bovine fetal skin cryopreserved by a simple method could be used as donors in nuclear transfer using the portable $CO_2$ incubator.

• 대한기계학회논문집A
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• 제29권7호
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• pp.970-975
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• 2005

This paper presents a novel microgenerator of $CO_2$ (carbon dioxide) gas. $NaHCO_3$ (sodium bicarbonate) in a chamber is decomposed by the underlaid microheater. Alternatively, water droplet is caged by paraffin layer and released by heating. The released water dissolve HOC(COOH)$(CH_2COOH)_2$ (citric acid) powder and then, $NaHCO_3$ reacts with the solubilized HOC(COOH)$(CH_2COOH)_2$ and $CO_2$ is produced. Micropumps actuated by $CO_2$ generation were fabricated. A portable micro cell incubator of which pH is controlled by the produced $CO_2$ is also presented as one of the further applications.