• 한국산림과학회지
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• 제103권1호
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• pp.59-64
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• 2014

본 연구에서는 물황철나무(Populus koreana Rehder) 집단을 대상으로 I-SSR 표지자를 이용해 유전다양성과 유전적 공간구조를 분석하였다. 물황철나무는 중국, 러시아 극동지역과 북한의 고산 계곡부 등에 서식하는 낙엽활엽 교목이다. 물황철나무는 남한에서 강원도 일대에 제한적으로 분포한다. 특히 오대산 집단은 물황철나무의 남방한계지로서 유전자원보존의 중요성이 강조된다. 8개의 I-SSR primer로 유전다양성을 추정한 결과, Shannon의 다양성 지수(I)는 0.230, 이형접합도의 기대치(He)는 0.151로 유사한 생활사를 갖는 타 수종에 비해 유전다양성이 매우 낮게 나타났다. 유전적 군락을 확인하기 위해 공간적 자기상관성 분석을 수행한 결과, 조사 지역 내의 물황철나무 집단은 400 m 이내에서 유전적으로 유사한 군락구조를 갖고 있는 것으로 나타났다. 물황철나무 집단의 현지외 유전자 보존을 위한 표본추출 시, 개체 간 거리를 400 m 이상으로 두는 것이 효율적일 것으로 판단된다.

• Journal of Plant Biotechnology
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• 제31권1호
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• pp.49-53
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• 2004

기공이 잎의 앞뒤 양면에 존재하는 생리ㆍ형태적 특징을 가지고 있는 외래 교잡종인 Peace (Populus koreana ${\times}$ P. trichocarpa)포플러의 효율적인 기내증식법을 개발하고자 액아와 엽육 절편을 이용하여 기내배양을 실시하였다. 몇 종류의 배지 시험을 통해 1/2MS배지를 적정배지로 선정할 수 있었고, 이 배지에 TDZ를 처리하여 multiple shoot 및 adventitious shoot유도 효과를 조사하였다. 절간액아 및 엽육을 절편으로 TDZ의 농도별 처리로 배양 2주 후부터 multiple shoot 및 adventitious shoot가 유도되었으며 절편 및 TDZ처리 농도에 따라 차이를 나타냈다. 액아로부터는 TDZ 처리로 절편당 5-7개의 multiple shoot가 유도된 반면 엽육 절편에서는 2-5개의 adventitious shoot가 유도되었다. 한편 부정아로부터 정상적 인 줄기의 성장에 있어서도 절편에 따른 차이를 나타냈다. 이상의 결과는 저농도의 TDZ의 처리로 peace포플러의 액아 혹은 엽육 절편의 기내배양을 통해 대량증식이 가능함을 보여주는 결과이다.

• 한국산림과학회지
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• 제98권5호
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• pp.513-523
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• 2009

Many species, cultivars, and hybrids in Populus have been introduced and developed by the scientists of Department of Forest Genetic Resources, Korea Forest Research Institute in Korea. P. alba ${\times}$ glandulosa, P. euramericana, P. nigra ${\times}$ maximowiczii, P. koreana ${\times}$ P. nigra var. italica, and P. davidiana have been studied from many aspects, i.e., genetics, breeding, physiology, propagation, silviculture, biomass production, biotechnology and phytoremediation. These precedent results will provide a sound basis for a newly-arising research on short-rotation coppice as one of renewable resources and phytoremediation plants. It was found that there were many promising varieties and clones for these purposes. However, minute analysis on specific gravity and caloric values for those plants should be followed under several silvicultural and rotational systems.

• 한국자원식물학회지
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• 제19권4호
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• pp.447-458
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• 2006

본 연구는 만덕산과 미륵봉주변의 자원식물의 분포현황과 활용가능성을 제시함으로써, 식물자원의 보전과 활용에 대한 기초자료를 제공하고자 수행하였다. 만덕산과 미륵봉주변의 자원식물을 조사한 결과, 107과 339속 481종 1아종 65변종 3품종으로 총 550종류(taxa)가 확인되었다. 이중 자원식물을 용도별로 구분하면 식용식물에는 323종류(58.7%), 약용식물에는 384종류(69.8%), 관상용식물에는 267종류(48.5%), 기타용 식물에는 268종류(48.7%)로 분석되었다. 희귀식물에는 말나리(Lilium distichum; 보존우선순위 159번), 뻐꾹나리(Tricyrtis dilatata; 97번), 쥐방울덩굴(Aristolochia contorta; 151번), 왕벚나무(Prunus yedoensis; 110번)가 확인되었으며, 특산식물에는 개비자나무(Cephalotaxus koreana), 지리대사초(Carex okamotoi), 털중나리(Lilium amabile), 은사시나무(Populus tomentiglandulosa), 키버들(Salix purpurea var. japonica), 다화개별꽃(Pseudostellaria multiflora), 왕벚나무(Prunus yedoensis), 개나리(Forsythia koreana), 오동(Paulownia coreana), 병꽃나무(Weigela subsessilis), 벌개미취(Aster koraiensis) 등의 11종류(taxa)가 확인되었다. 또한, 식물구계학적 특정식물종에는 V등급에 해당되는 식물로 식재종인 왕벚나무, IV등급에는 등나무, III급에는 꼬리조팝나무, 단풍나무, 갈퀴아재비 3종류, II등급에는 돌양지꽃, 민둥뫼제비꽃, 층꽃나무 3종류, 그리고 I등급에는 잣나무(식재), 일월비비추, 홀아비꽃대, 왕버들, 가래나무 등 22종류로 분석되어 총 30종류(5.5%)가 확인되었다. 본 조사지역은 외래식물 식재나 야생화 채취 및 토양 답압등 인위적인 훼손우려가 점차적으로 증가하고 있는 실정이므로 시급한 대책 마련이 필요할 것으로 판단된다.

• Journal of Plant Biotechnology
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• 제6권1호
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• pp.15-19
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• 2004

The expression of a chimeric transgene (nos-npt II) has been examined in 9 years old transgenic poplars (Populus koreana x P. nigra) growing in a nursery. The expression of the gene in twenty six independentely transformed plants were examined by 1) enzyme (NPT II) assay, 2) RT-PCR, and 3) resistance to kanamycin. High NPT II activities in young leaves of all the transformed plants were found even without a selection pressure for antibiotics for 9 years. However, the activity varied with the positions of leaves in the stem in that young leaves showed higher activity than did mature tissues. When leaf segments were cultured in the presence of 150 mg/l kanamycin, only those from young leaves produced vigorously growing callus. However, as in the case of NPTII assay, the leaf segments from mature leaves did not form callus well on the media. RT-PCR with nptII specific primers also showed that amplification products were observed only when RNAs from young tissues were used. The total RNA gel showed that while RNA in young leaves are relatively stable and in a large quantity, those in old leaves were mostly degraded. All the above results suggest that the gene is transcriptionally active only in young tissue even though it is attached to a constituitive promoter. Therefore, the expression of foreign gene in poplar plants seemed to be affected by the metabolic state of the cells and thus vary greatly with the developmental stages and the age of tissue.

• The Korean Journal of Ecology
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• 제18권2호
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• pp.255-263
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• 1995

Two poplar clones, I-214 (Populus euramericana) and Peace (P. Koreana × P. trichocarpa), were grown for 21 days in growth chambers at different CO₂concentrations (350, 700 and 2,000 μL·L-1). I-214 has stomata responding to environmental conditions in normal ways and Peace has unresponsive stomata to environmental factors including light, ABA, water stress and CO₂. In both plants, elevated CO₂ stmulated the growth of plant parts, especially leaf dry weight. And a CO₂ enrichment of 700 μL·L-1 CO₂ caused increment of net assimilation rate (NAR). The growth responses of these plants to CO₂ enrichment were different especially at high CO₂ condition (2,000 μL·L-1 CO₂). The total dry weight in Peace increased up to 2,000 μL·L-1 CO₂ but not in I-214. A CO₂ enrichment of 2,000 2,000 μL·L-1 CO₂ had little effect on NAR of I-214 but enhanced NAR of Peace. Although it is uncertain whether the different responses to CO₂ enrichment between I-214 and Peace resulted from the different properties of stomatal responses to long-term CO₂ treatment, the decrease in NAR is probably due in part to CO₂-induced stomatal closure in I-214 but not in Peace.

• 한국환경복원기술학회지
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• 제10권2호
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• pp.44-53
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• 2007

The vascular plants of the studied area in the Gyehwado was listed 378 taxa; 99 families, 259 genera, 322 species, 1 subspecies, 50 varieties and 5 forms. In this, Woody plants were 130 taxa (34.4%), Herbaceous plants were 248 taxa (65.6%) of all 378 taxa vascular plants. Pteridophyta were 7 families, 9 genera, 11 taxa (2.9%), Gymnospermae were 4 families, 5 genera, 9 taxa (2.4%) and Angiospermae were 88 families, 245 genera, 358 taxa (97.7%). Angiospermae in this, Monocotyledoneae were 10 families, 45 genera, 60 taxa (16.8%) and Dicotyledoneae were 78 families, 200 genera, 298 taxa (83.2%). Based on the list of the rare plants by the Forest Research Institute, recorded in the studied areas; Phacelurus latifolius (Preservation priority order; No. 194), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and based on the list of Korean endemic plants, 6 taxa were recorded; Cephalotaxus koreana, Populus tomentiglandulosa, Carpinus coreana, Indigofera koreana, Forsythia koreana, Weigela subsessilis. Specific plant species by floral region were total 30 taxa; Wistaria floribunda in class IV, 5 taxa (Ligustrum ovalifolium, Callicarpa mollis, Lonicera subhispida, etc.) in class III, 24 taxa (Cephalotaxus koreana, Aristolochia contorta, Grewia biloba var. parviflora, etc.) in class I. The naturalized plants in this site were 12 families, 27 genera, 34 species, 2 varieties, 36 taxa and naturalization rate was 9.5% of all 378 taxa vascular plants.

• 한국환경과학회지
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• 제16권10호
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• pp.1139-1146
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• 2007

The vascular plants in the Mt. Manduk was listed 560 taxa composed of 110 families, 345 genera, 488 species, 1 subspecies, 67 varieties and 4 forms. Based on the list of the rare plants by the Forest Research Institute, 4 taxa were recorded in the studied areas; Lilium distichum (Preservation priority order; No. 159), Tricyrtis dilatata (No. 97), Aristolochia contorta (No. 151) and Prunus yedoensis (No. 110). Based on the list of Korean endemic plants, 12 taxa were recorded; Cephalotaxus koreana, Carex okamotoi, Lilium amabile, Populus tomentiglandulosa, Salix purpurea var. japonica, Pseudostellaria multiflora, Prunus yedoensis, Stewartia koreana, Forsythia koreana, Paulownia core ana, Weigela subsessilis and Aster koraiensis. Based on the list of approved for delivering overseas of plants, 7 taxa were recorded; Carex okamotoi, Lilium distichum, Aristolochia contorta, Vaccinium oldhami, Paulownia coreana, Asperula lasiantha and Saussurea seoulensis. Specific plants by floral region were total 32 taxa; Prunus yedoensis in class V, Wistaria floribunda in class IV, 5 taxa (Girardinia cuspidata, Spiraea salicifolia, Acer palmatum, Stewartia koreana, Asperula lasiantha) in class III, 3 taxa (Potentilla dickinsii, Viola tokubuchiana var. takedana and Caryopteris incana) in class II and 22 taxa (Pinus koraiensis, Hosta capitata, Chloranthus japonicus, Salix glandulosa, Juglans mandshurica, etc.) in class I. The naturalized plants in the surveyed sites were 14 families, 36 genera, 44 species, 2 varieties, 46 taxa and naturalization rate was 8.2% of all 560 taxa of vascular plants. Wild plants disturbing ecosystem like Ambrosia artemisiifolia var. elatior have been increasing. Therefore, continuous control and conservation measures are needed on the ecosystem of Mt. Manduk.

• 농업생명과학연구
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• 제45권5호
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• pp.17-23
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• 2011

간척지에 식재된 포플러 10클론을 대상으로 생육특성 및 적응능력을 평가하였다. 시험림 토양의 전기전도도(EC)는 0.51 dS/m로 높지 않음에도 불구하고 $Na^+$, $Ca^{2+}$ 및 $Mg^{2+}$ 함량은 일반 토양에 비해 각각 10.0배, 3.4배 및 1.5배 높았으며, 유기물과 총 질소 함량은 각각 22.9배, 23.0배 낮았다. 식재 후 3년째 포플러 클론의 평균 생존율은 88%로 양호하였으며, Eco28, I-476, Dorskamp 클론이 생존율 100%로 가장 우수하였고, 97-19, Suwon 클론이 상대적으로 낮았다. 전체 클론의 평균 수고와 흉고직경은 각각 4.8 m와 3.6 cm로 나타났으며, 전체 10클론 중 Dorskamp 클론의 수고와 흉고직경 생장은 각각 5.9 m 및 5.0 cm로 가장 우수하였다. 간척지 환경에서 조기낙엽과 병해충 등 가시적 피해는 97-19 및 Dorskamp 클론이 가장 적었으며, Suwon 및 I-476 클론은 피해가 많은 것으로 밝혀졌다. 생존율, 생장, 가시적 피해를 대상으로 평가한 간척지 적응능력은 Dorskamp 클론이 가장 우수하게 나타났고, Suwon 클론이 가장 낮은 것으로 평가되었다.

• 한국환경복원기술학회지
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• 제11권4호
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• pp.37-45
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• 2008

The vascular plants of the studied area in Nonsan economical forest growing site appeared to be 341 taxa; 81 families, 218 genera, 290 species, 1 subspecies, 42 varieties and 9 forms. Based on the list of Korean endemic plants, 5 taxa existed; Cephalotaxus koreana, Thalictrum actaefolium, Asperula lasiantha, Populus tomentiglandulosa (Planted species), Paulownia coreana (Planted species). Specific plant species by floral region were total 13 taxa; Cirsium setidens in class III, Potentilla dickinsii in class II, Cephalotaxus koreana, Pinus koraiensis (Planted species), Hosta capitata, Juglans mandshurica, Alnus hirsuta, Quercus variabilis, Ulmus parvifolia, Dictamnus dasycarpus, Gwenia biloba var. parviflora, Vaccinium oldhami, Trigonotis icumae in class I. The naturalized plants in this site were 9 families, 19 genera, 20 species, 1 varieties, 21 taxa and naturalization rate was 6.1% of all 341 taxa vascular plants. The communities were classified into Quercus acutissima community, Pinus densiflora community, Quercus variabilis community, Platycarya strobilacea community, Quercus aliena community of natural forests and Pinus rigida community, Metasequoia glyptostroboides community, Larix leptolepis community of artificial forests.