• Journal of Korean Society of Forest Science
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• v.64 no.1
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• pp.1-10
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• 1984

Two fast-growing clones 65-29-19 and 66-6-8 and two less fast-growing clones 66-14-29 and 66-14-99 were compared at intensively cultured plots to identify what morphological characters are related to fast growth and high biomass yield of Populus alba ${\times}$ Populus glandulosa $F_1$. The plantations were established in the spring of 1982 and 1983 at the Seoul National University nursery in Suweon. Differences in dry matter production were found among two-year-old clones but not, among one-year-old clones. Highly significant correlations were found between total leaf area per tree and total dry weight per tree and between total branch length per tree and total dry weight per tree for two-year-old clones. This relation was more obvious during the late growing season. Morphological characters desirable for producing high biomass identified in fast-growing clones were as follows; high shoot-root ratio, high leaf surface area per tree, long leaf retention period, and many small leaves and compact crown architecture in the upper portion of true crown.

• Journal of the Korean Wood Science and Technology
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• v.31 no.4
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• pp.31-37
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• 2003

Flat-sawn 32 mm-thick boards of Populus alba×P. glandulosa, one of major plantation species in this country, were dried in conventional, high-temperature and microwave-vacuum (MW/V) kilns. The average green specific gravity of the specimens used in this study was 0.349±0.074. Their average green moisture contents of heartwoods and sapwoods were around 200% and 100%, respectively. From green to oven-dry they shrunk 2.4 and 7.3% in radial and tangential directions, respectively, and there was no discrepancy between heartwoods and sapwoods. It took 65, 35 and 22 hours to dry from green to 10% moisture content in conventional, high-temperature and MW/V kilns, respectively. A colorimetry study showed that the surface color of the specimens dried in a MW/V kiln was clearer and lighter than those in other kilns.

• Journal of Korean Society of Forest Science
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• v.90 no.6
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• pp.734-741
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• 2001

The two-month-old rooted-cuttings of Populus alba ${\times}$ glandulosa, P. euramericana and P. nigra ${\times}$ maximowiczii clones were exposed to livestock waste water - one of major water pollutants, and ground water in order to determine the effects of livestock waste water on growth response and absorption capacity of the species. For this purpose, 5 clones of each species were used. In all the species, the height growth of rooted-cuttings was better in livestock waste water treatment than in ground water. Of all the poplar species compared, the height growth was best in P. alba ${\times}$ glandulosa. In the cases of P. euramericana and P. nigra ${\times}$ maximowiczii, the height growth in the livestock waste water treatment was statistically different among clones, whereas there was no significant difference among P. alba ${\times}$ glandulosa clones. Aboveground biomass such as leaf and shoot dry weight of all the species increased in the livestock waste water treatment, while root dry weight decreased. In addition, chlorophyll contents in leaf of all the poplar species increased in the livestock waste water treatment. All the poplar trees showed temporal variation in the absorption amount of livestock waste water during the experimental period. In all the poplar species, the absorption amount of livestock waste water was less than that of ground water. Of 3 poplar species, P. alba ${\times}$ glandulosa was best in the absorption capacity of livestock waste water. Of all 15 poplar clones compared in this study, the 72-16 clone of P. alba ${\times}$ glandulosa showed the best absorption capacity.

• Journal of Korean Society of Forest Science
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• v.73 no.1
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• pp.33-42
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• 1986

This study was carried out to investigate the optimum conditions for isolation and culture of mesophyll protoplasts from Populus alba ${\times}$ P. glandulosa. The results obtained from the experiments are as follows; 1) The suitable concentration of BAP for shoot multiplication was 0.4 mg/l. 2) High yield and viability of isolated protoplasts were obtained by our high enzyme-short time incubation method. 3) Optimum enzyme concentrations for mesophyll protoplast isolation were Cellulase 2%, Macerozyme 0.8%, Hemicellulase 1.2%, Driselase 2%, and Pectolyase Y-23 0.05%. 4) 0.6M mannitol in enzyme solution was the most effective for protoplast isolation and viability. 5) The most adequate pH level of enzyme solution was pH 5.6. 6) The effect of DTT and MES buffer was significant. 7) For protoplast purification, 0.6M sucrose was the most proper concentration. 8) The adding effect of Dextran T40 in floating solution was important. 9) The mesophyll protoplasts isolated through our high enzyme-short time incubation method revealed successful response to culture condition over 3 weeks of culture.

• Journal of Korean Society of Forest Science
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• v.70 no.1
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• pp.63-71
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• 1985

To sled the superior clones of Populus alba ${\times}$ Populus glandulosa $F_1$, growth and some growth-related enzyme activities were examined for thirteen, two-year-old and fifteen, three-year-old trees at Seoul National University nursery in Suwon. Clonal differences in total dry weight per tree and leaf surface area per tree were significant at the 5% level. Significant correlations were found between total dry weight per tree and leaf surface area per tree (r=0.875), between leaf peroxidase activity per tree and total dry weight per tree (r=0.854), and between leaf nitrate reductase activity per tree arid total dry weight per tree (r=0.914). Leaf peroxidase and nitrate reductase activities per unit fresh weight of one-year-old tree increased with increasing leaf order numbers basipetally, reaching maximum values in the eighteenth and thirty-third leaves, respectively, and decreased gradually from those leaves to basipetal lower leaves. Clones 65-29-19, 66-15-3, 65-22-11, 66-14-93, and 66-26-55 among two-year-old trees, and clones 64-6-44, 66-14-29, 66-26-55, 65-22-11, and 68-1-54 among three-year-old trees showed greater leaf surface areas, peroxidase and nitrate reductase activities per unit leaf fresh weight than other clones, Growth of Populus alba ${\times}$ Populus glandulosa $F_1$ clones might be estimated from either leaf surface area per tree or peroxidase and nitrate reductase activities per tree. Therefore, measurements of leaf surface area and leaf enzyme activities appear useful to select superior Populus clones at early growth stages.

• Journal of Korean Society of Forest Science
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• v.87 no.2
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• pp.153-163
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• 1998

Genetic relationships of some poplars in the section Leuce, including 5 species and 11 clones of Populus alba${\times}$glandulosa, were investigated on the basis of RAPD marker analysis. Twenty-two of the 88 arbitrary 10-mer primers, showed reproducible amplification in the preliminary experiment with 6 samples, were used for PCR and generated a total of 181 RAPD markers. Genetic relationships among the analyzed samples were tested by two phenetic methods of the UPGMA and the neighbor-joining, which revealed the close genetic relationship between P. glandulosa and P. alba. And the close genetic relationship between P. glandulosa and P. davidiana was ascertained by the principal component analysis. Based on the observation of the close genetic relationship between them, it was deduced that P. glandulosa might be originated by the saltational speciation caused by the hybridization between P. alba and P. davidiana in nature.

• Journal of Korean Society of Forest Science
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• v.59 no.1
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• pp.15-30
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• 1983

To select the superior clones of Populus alba ${\times}$ Populus glandulosa $F_1$, growth and some growth-related physiological characters were examined for thirteen, one-year-old and fifteen, two-year-old clones, respectively at the Seoul National University nursery in Suweon, Clonal differences in growth (total dry weight per tree), leaf area per tree and leaf chlorophyll content per tree at tow-year-old plots were highly significant at the 1% level, but not at one-year-old plots, Highly significant correlation was found between leaf area per tree and total dry weigh per tree (r=0.865), between leaf chlorophyll content per tree and total dry weight per tree (r=0.888), and between photosynthetic ability per tree and total dry weight per tree (r=0.745). The photosynthetic ability and leaf chlorophyll content per unit leaf area of one-year-old plant increased with increasing leaf order number from top. reached maximum value in the twenty-eithth and thirty-third leaves, respectively, and then decreased gradually from those leaved to base, Clones 68-1-54, 66-26-55 and 65-22-11 showed test growth based on leaf area per tree, leaf chlorophyll content per unit leaf area and photosynthetic ability of leaf tissue per unit area. Growth yield of populus alba ${\times}$ P. glandulosa $F_1$ clones could be estimated from either leaf area per tree of leaf chlorophyll content per tree. Therefore, measurements of leaf area and leaf chlorophyll content appear useful to select superior populus clones early growth.

• Journal of Korean Society of Forest Science
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• v.95 no.6
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• pp.743-750
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• 2006

Poplars and willow were planted to identify suitable species and varieties for landfill reclamation at the Sudokwon Landfill Site in 1997. Survival rate, growth performance, vitality, visible foliar injury by pollutants, fungi, and leaf insects, and stem borer damage have been investigated for 10 clones of 4 poplar species and 2 clones of one willow species from 1997 to 2005. The average survival rates of poplar and willow clones were drastically decreased from 90% in 1997 to 53% in 2005. Among poplar species, Populus alba ${\times}$ P. glandulosa showed the highest mean survival rate of 66%, while Populus koreana ${\times}$ P. nigra var. italica and Populus euramericana were the lowest of 41%, respectively in 2005. Clivus, which is one of the clones from Populus alba ${\times}$ P. glandulosa, showed the highest survival rate of 73%. For mean height, Ec028 clone(P. euramericana) showed the highest of $11.2m{\pm}2.1m$ and followed by Clivus of $11.0m{\pm}2.0m$. Clone 131-27(Salix alba) was the lowest of $7.8m{\pm}1.6m$. Vitality, defoliation, visible foliar damage, and stem borer damage were significantly different among clones. Most of Populus alba ${\times}$ P. glandulosa and Salix alba clones seemed to have strong vitality and to be tolerant to various stresses at the site. However, Populus nigra ${\times}$ P. maximowiczii was sensitive to the stress. We have selected 5 clones in total: Clivus as the best clone for waste landfill reclamation, and additionally two Salix clones 131-25, 131-27 and two clones of Populus alba ${\times}$ P. glandulosa (72-9, 72-16) have been selected. These five clones could be supplied for planting at sites having an environment similar to the Sudokwon Landfill Site.

• Journal of the Korean Wood Science and Technology
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• v.20 no.1
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• pp.60-71
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• 1992

To investigate the delignification behaviour in solvolysis pulping process, Populus alba ${\times}$ glandulosa H. and Pinus Kuraiensis S. et Z. were cooked with p-cresol and vater solvent(2:8, 5:5, 8:2 v/v) at $175^{\circ}C$ for 9 cooking time levels(20, 40, 60, 80, 100, 120, 140, 160, 180, min). Pulp yield, residual lignin content, de lignification rate, decarborhydration rate were determined. Delignification behaviours were analyzed by TEM. 1. The p-cresol-water solvent cooking of P. alba ${\times}$ glandulosa showed good delignification at the solvent system which the mixture ratio of p-cresol and water were 2:8(v/v), while the cooking of P. koraiensis with the p-cresol and water mixture ratio of 5:5 was no good. 2. P. alba ${\times}$ glandulosa showed three step-delignification phenomena at the solvent system which the mixture ratio of p-cresol and water were 2:8(v/v) anti 5:5(v/v). But P. koraiensis showed a first order delignification reaction at the same mixture ratio of p-cresol and water solvent system. 3. In TEM micrograph obtained for the solvent system which the mixture ratio of p-cresol and water was 5:5(v/v), the partial delignification of the cell corner of P. alba ${\times}$ glandulosa and P. koraiensis were observed at 60min. of cooking time. Complete delignification at the cell corner of P. alba ${\times}$ glandulosa was observed at 160min. and that of P. koraiensis was observed of 180min. of cooking time. 4. In optical microscopic observation, fiber separation of P. alba ${\times}$ glandulosa occured at 120min. and that of P. koraiensis began at 140min. of cooking time. 5. At the solvent system which the mixture ratio of p-cresol and water was 5:5(v/v), middle layer on secondary wall($S_2$) and cell corner of P. alba ${\times}$ glandulosa were more selectively delignified than primary wall(P) and outer layer on secondary wall($S_1$). However P. koraiensis did not showed any difference in delignification between cell wall layers and cell corner.

• Journal of Korean Society of Forest Science
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• v.70 no.1
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• pp.72-76
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• 1985

Populus alba ${\times}$ P. glandulosa cuttings in nursery bed were inoculated with mycelium of ectomycorrhizal fungus, Pisolithus tinctorius (Pt) to evaluate effectiveness of the fungus in growth stimulation of poplar. Pt was cultured in 1l glass bottles with vermiculite-peatmoss mixture moistened with modified Melin-Norkrans' solution. The nursery bed was arranged for microplots of $1{\times}2m$ in size and fumigated with methyl bromide before inoculation and cutting placement. Fifty cuttings were placed in each microplot and two treatments (fumigation only and fumigation plus Pt inoculation) were replicated three times. At the end of the first growing season, inoculated plants grew 19% faster in height and produced 49% more dry weight (above-ground portion) than uninoculated plants. Survival rate of inoculated cuttings was also improved by 20% over that of uninoculated cuttings. Inoculated cuttings developed abundant fine root system with golden brown zigzag tips. In the middle of September a sporocarp of Pt was produced from an inoculated plot, suggesting successful establishment of mycorrhiza between poplar and Pt fungus.