• 제목/요약/키워드: Population genetic diversity

검색결과 606건 처리시간 0.029초

I-SSR 분석에 의한 노각나무 천연집단의 유전변이 (Genetic Variation in the Natural Populations of Korean Stewartia (Stewartia koreana Nakai) Based on I-SSR Analysis)

  • 양병훈;구영본;박용구;한상돈
    • 한국자원식물학회지
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    • 제19권1호
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    • pp.189-195
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    • 2006
  • 본 연구는 우리나라 특산수종이며 조경 및 원예적 가치가 높은 노각나무 유전변이를 조사하기 위해 6개 천연집단을 선발하여 DNA I-SSR 표지자를 사용, 유전다양성 및 유전구조를 조사하였다. 5개의 I-SSR primer(#813, 815, 818, 820, 823)에서 총 61개의 증폭산물을 관찰할 수 있었으며, 유전 다양성을 나타내는 P(Percentage of polymorphic loci)값과S.I.(Shannon's information Index)가 남쪽에 분포하는 오봉산(P=88.5%, S.I.=0.467), 금산(P=86.9%, S.I.=0.427), 바랑산(P=83.6%, S.I.=0.425)집단이 높았으며, 북쪽(내륙)에 분포하는 소백산(P=80.3%, S.I.=0.396), 지리산(P=77.1%, S.I.=0.368), 가야산(P=75.4%, S.I.=0.358)집단은 낮았다. 전체 유전변이 중 11.8%만이 집단간에 기인하는 것으로 나타났고, 나머지 88.2%는 집단내 개체간의 차이에서 기인하였다. 유전거리를 이용하여 UPGMA법에 의한 유집분석을 실시한 결과 지리적 분포에 대한 뚜렷한 경향은 나타나지 않았다.

희귀수종 먹넌출 엽의 형태적 특성과 유전변이 (Characteristics of Leaf Morphology and Genetic Variation of the Rare Woody Plant, Berchemia racemosa var. magna)

  • 송정호;임효인;장경환;한진규
    • 한국자원식물학회지
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    • 제26권5호
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    • pp.613-618
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    • 2013
  • 우리나라에서 안면도에만 분포하는 먹넌출 집단의 생명자원보존을 위하여 엽의 형태적 특성과 I-SSR 표지자를 이용한 유전변이를 조사하였다. 10가지 엽특성에 대한 ANOVA 분석결과 모든 특성에서 개체 간에 통계적인 유의성이 인정되었다. 조사된 39개체의 평균특성은 엽신장 11.8 cm, 최대엽폭 7.1 cm, 엽지수 1.67, 상1/3폭 5.4 cm, 하1/3폭 6.2 cm, 엽병길이 3.6 cm, 엽두께 0.19 mm, 엽맥수(좌) 11.5개, 엽맥수(우) 11.4개, 엽면적 61.7 $cm^2$로 나타났다. 변이계수 값은 엽두께, 엽병길이, 엽면적이 각각 18.8%, 21.7%, 22.0%로 높게 나타났으며, 나머지 특성들에서는 15% 이내의 비교적 낮은 변이를 나타냈다. 선발된 8개 I-SSR Primer에서 총 50개의 증폭산물을 얻었으며, 유효대립 유전자의 수 1.719개, 다형적 유전자좌의 비율 26.0%, 이형접합도의 기대치 0.410 및 Shannon의 다양성지수 0.598로 각각 나타났다. 안면도 먹넌출 집단은 제한된 지역에 분포하며 개체수가 적음에도 불구하고 높은 유전다양성을 유지하는 것으로 나타났다.

Analysis of Genetic Diversity and Population Structure of Buckwheat (Fagopyrum esculentum Moench) Landraces of Korea Using SSR Markers

  • Song, Jae-Young;Lee, Gi-An;Yoon, Mun-Sup;Ma, Kyung-Ho;Choi, Yu-Mi;Lee, Jung-Ro;Jung, Yeon-Ju;Park, Hong-Jae;Kim, Chung-Kon;Lee, Myung-Chul
    • 한국자원식물학회지
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    • 제24권6호
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    • pp.702-711
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    • 2011
  • Buckwheat (Fagopyrum esculentum Moench), one of the minor crops grown in Korea belonging to the Polygonaceae family, is an annual crop widely cultivated in Asia, Europe, and America and has a character of outcrossing and self-incompatibility. The objective of this study was to analyze the genetic variability, phylogenetic relationships and population structure of buckwheat landraces of Korea using SSR markers. Ten microsatellite markers have been detected from a total of 79 alleles among the 179 buckwheat accessions were collected from Korea. The number of allele per marker locus ($N_A$) ranged from 2 (GB-FE-001, GB-FE-043 and GB-FE-055) to 31 (GB-FE-035) with an average of 7.9 alleles. GB-FE-035 was the most polymorphic with the highest PIC value 0.93. Major allele frequencies ($M_{AF}$) for the 10 polymorphic loci varied from 0.12 to 0.97 with a mean allele frequency of 0.57. The expected heterozygosity ($H_E$) values ranged from 0.05 to 0.94 with an average of 0.53. The observed heterozygosity ($H_O$) ranged from 0.06 to 0.92 with an average of 0.42. The overall polymorphic information contents (PIC) values ranged from 0.05 to 0.93 with an average of 0.48. The landrace accessions of buckwheat used in the present study were not distinctly grouped according to geographic distribution. The study concludes that the results revealed genetic differentiation was low according to the geographic region because of outcrossing and self-incompatibility. We reported that our analyses on the genetic diversity of common buckwheat cultivars of Korea were performed by using of microsatellite markers.

Evaluation of Genetic Structure of Amaranth Accessions from the United States

  • He, Qiang;Park, Yong-Jin
    • Weed & Turfgrass Science
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    • 제2권3호
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    • pp.230-235
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    • 2013
  • Amaranths (Amaranthus sp.), an endemic American crop, are now grown widely across the world. This study used 14 simple sequence repeat (SSR) markers to analyze the genetic diversity of 74 amaranth accessions from the United States, with eight accessions from Australia as controls. One hundred twenty-two alleles, averaging eight alleles per locus, were observed. The average major allele frequency, expected heterozygosity, and polymorphism information content (PIC) were 0.44, 0.69, and 0.65, respectively. The structure analysis based on genetic distance classified 77 accessions (94%) into three clusters, while five accessions (6%) were admixtures. Among the three clusters, Cluster 3 had the highest allele number and PIC values, while Cluster 2 had the lowest. The lowest FST was between Clusters 1 and 3, indicating that these two clusters have higher gene flow between them compared to the others. This finding was reasonable because Cluster 2 included most of the Australian accessions. These results indicated satisfactory genetic diversity among U.S. amaranths. These findings can be used to design effective breeding programs involving different plant characteristics.

Taxonomic Review of the Genus Echinochloa in Korea (II): Inferred from Simple Sequence Repeats

  • Lee, Jeongran;Kim, Chang-Seok;Lee, In-Yong
    • Weed & Turfgrass Science
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    • 제3권3호
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    • pp.190-195
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    • 2014
  • Echinochloa (L.) P. Beauv. includes some of the noxious weeds, causing a serious yield loss when they are dominant in the fields. Identification of the Echinochloa is very difficult because many interspecific and intraspecific forms of the species are found. However, it is important to identify the species exactly and to know the genetic diversity of the species for effective weed management. This study was conducted to identify and summarize the Echinochloa species by comparing the genetic variation and relationship among Korean Echinochloa species using SSR. The genetic diversity of 107 individuals, including seven species were assessed using five SSR markers. UPGMA dendrogram generated two clades (I and II) and clade II divided again into two subclades (II-1 and II-2) whereas the model based genetic structure proposed four subpopulations. The two subpopulations were corresponded to clades I and II-1 and the other two were arranged to clade II-2 of the UPGMA dendrogram. We have concluded that E. colona and E. glabrescens might have not distributed in Korea. The biological varieties, praticola and echinata, of E. crus-galli should be treated as E. crus-galli. Korean Echinochloa should be summarized with four species, i.e., E. oryzicola, E. crus-galli, E. esculenta, and E. oryzoides.