• Animal cells and systems
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• 제1권1호
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• pp.129-134
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• 1997

HLA-A2 is one of the most diversified HLA-class I antigen with 17 subtypes so far identified at the molecular level. HLA-A*02 subtyping has significant implications on the tissue typing for organ and bone marrow transplantations. Recently, DNA-based typing methods have been successfully applied to the elucidation of HLA gene polymorphisms. In the present study, HLA-A*O2 genotyping was established by using nested polymerase chain reaction-sequence specific primers (PCR-SSP) and distribution of A*O2 alleles were determined in Korean individuals. Genomic DNA prepared from four B-lymphoblastoid cell lines and lymphocytes from serologically defined 48 HLA-A2 Korean individuals by phenol/chloroform extractions was typed. The results of the four B-lymphoblastoid cells were consistent with the previous data typed by PCR analysis. Five A*O2 alleles-A*0201, A*0203, A*0206, A*0207 and A*0210-were commonly observed in a total of 17 A*02 alleles. Of these, A*0207 (f=49.0%) was the most frequent allele in Korean population. A*0206 (f=28.3%) and A*0201 (f=17.0%) were also found frequently while A*0203 and A*0210 types were observed in less than 5%. In conclusion, the high level of discrimination for HLA-A*O2 alleles will prove useful and informative in the study of transplant survival, and may identify the importance of allelic differences not readily detectable by serology on host and donor compatibility.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1725-1731
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• 2020

Objective: An initial RNA-Sequencing study revealed that UDP-galactose-4-epimerase (GALE) was one of the most promising candidates for milk protein concentration in Chinese Holstein cattle. This enzyme catalyzes the interconversion of UDP-galactose and UDP-glucose, an important step in galactose catabolism. To further validate the genetic effect of GALE on milk protein traits, genetic variations were identified, and genotypes-phenotypes associations were performed. Methods: The entire coding region and the 5'-regulatory region (5'-UTR) of GALE were re-sequenced using pooled DNA of 17 unrelated sires. Association studies for five milk production traits were performed using a mixed linear animal model with a population encompassing 1,027 Chinese Holstein cows. Results: A total of three variants in GALE were identified, including two novel variants (g.2114 A>G and g.2037 G>A) in the 5'-UTR and one previously reported variant (g.3836 G>C) in an intron. All three single nucleotide polymorphisms (SNPs) were associated with milk yield (p<0.0001), fat yield (p = 0.0006 to <0.0001), protein yield (p = 0.0232 to <0.0001) and protein percentage (p<0.0001), while no significant associations were detected between the SNPs and fat percentage. A strong linkage disequilibrium (D' = 0.96 to 1.00) was observed among all three SNPs, and a 5 Kb haplotype block involving three main haplotypes with GAG, AGC, and AGG was formed. The results of haplotype association analyses were consistent with the results of single locus association analysis (p<0.0001). The phenotypic variance ratio above 3.00% was observed for milk protein yield that was explained by SNP-g.3836G >C. Conclusion: Overall, our findings provided new insights into the polymorphic variations in bovine GALE gene and their associations with milk protein concentration. The data indicate their potential uses for marker-assisted breeding or genetic selection schemes.

• 한국어류학회지
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• 제20권4호
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• pp.248-254
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• 2008

우리나라의 주요 양식 대상종인 넙치에 있어 유전적 조성에 대한 고려없이 구성된 양식 넙치 어미 집단에서 세대의 경과에 따른 집단의 유전적 다양성의 변화를 알아보기 위하여 총 8개의 microsatellite DNA markers를 이용하여 분석하였다. 동일한 수정란 생산업체에서 2003년에 생산된 양식 넙치와, 동일한 집단내에서 생산된 넙치로 어미 그룹이 완전히 교체된 2006년 및 2007년에 생산된 넙치 집단을 비교한 결과, 대립유전자 수와 이형접합기대치의 비교에서 2003년산 집단의 경우 9.75개와 0.796에서 2006년 7.78개와 0.785로 유전적 다양성이 다소 감소되는 경향이었다. 또한 대립유전자의 빈도에 있어서도 몇몇 대립유전자의 경우 소실되거나 빈도에 변화를 보이는 등 왜곡되어 나타났다. 그러나 여러 가지 다양성 지표들에서 수치상 감소를 나타내고 있는 이러한 결과들은 비록 유의적인 수준에서 유전적 다양성의 축소라고는 판단되지 않지만 양식 넙치 집단이 HWE에 어긋나 있어 세대가 진행될수록 집단의 유전적 다양성은 감소될 것으로 예상되었다.

• Journal of Animal Science and Technology
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• 제62권6호
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• pp.777-789
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• 2020

Intense artificial selection has been imposed to Luzhong mutton sheep population in the past years. Improvements on growth and reproductive performance are two breeding goals in the present herd. Although some progresses were phenotypically observed possibly due to inbreeding induced by strong selection in terms of these traits, the genomic evaluation was poorly understood. Therefore, a high-density SNP array was used to characterize the pattern of runs of homozygosity (ROH), estimate inbreeding and inbreeding depressions on early growth performance and litter size based upon ROH, and scan positive selection signatures of recent population. Consequently, a low inbreeding level was observed which had negative effects on litter size, but not on early growth performance. And 160 genes were under selection, of which some were reported to be linked to several traits of sheep including body weight, litter size, carcass and meat quality, milk yield and composition, fiber quality and health, and the top genes were associated with growth (growth hormone [GH]- growth hormone receptor [GHR]- Insulin-like growth factor 1 [IGF1] axis) and litter size (bone morphogenic proteins [BMPs]-associated). The effectiveness of previous breeding measures was highlighted, but purging selection was proposed to alleviate the inbreeding depression on litter size, providing some genomic insights to breeding management of Luzhong mutton sheep.

• 전자공학회논문지B
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• 제32B권12호
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• pp.1680-1686
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• 1995

GAs, effective stochastic search algorithms based on the model of natural evolution and genetics, have been successfully applied to various optimization problems. When population size is not large, GAs often suffer from the phenomenon of premature convergence in which all chromosomes in the population lose the diversity of genes before they find the optimal solution. In this paper, we propose that a new heuristic that maintains the diversity of genes by adding some chromosomes with random mutation and selective mutation into population during evolution. And population size changes dynamically with supplement of new chromosomes. Experimental results for several test functions show that when population size is rather small and the length of chromosome is not long, this method is effective.

• 한국발생생물학회지:발생과생식
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• 제21권4호
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• pp.435-440
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• 2017

The author performed PCR-based genetic platform to measure the hierarchical dendrogram of Euclidean genetic distances of Korean scallop populations (KSP), particularly for Chlamys farreri, which was further compared with those of the Chinese scallop populations (CSP), by employing the with specifically designed oligonucleotide primer sets. The scallop is economically and ecologically very important bivalves in South Korea. Relatively, individuals of KSP population were fairly distantly related to that of CSP population, as shown in the hierarchical dendrogram of Euclidean genetic distances. Comparatively, individuals of KSP population were fairly distantly related to that of CSP population. Thus analysis of genetic difference between scallop populations could provide important statistics for fishery and aquaculture. Overall the results showed specific and/or conserved genetic loci between scallop populations. Information on the genetic distance of the bivalve would be helpful to understand scallop expansion or conservation in the coastal regions of South Korea. Specific markers developed by the author will be useful for the analysis of scallop population genetics and distribution in coastal region.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권1호
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• pp.117-121
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• 2004

Soluble proteins of silkworm hemolymph were investigated by means of isoelectric focusing (IEF). The protein spectra during ontogenesis of races and inter-races hybrids kept in Bulgaria was studied. A total of 51 protein bands in the hemolymph from fourth larval instar to imago were ascertained. Stage specific expression was established. The specific expression of some protein bands in the individual spectra manifest phenotype of gene determinate polymorphism (HP F, HP J, HP K, HP L, HP Q - in the zone with pH gradient 3.5-6.2 and HP K, HP L, HP N, HP P, HP T - in the zone with pH gradient 9.5 - 6.2). Breed specific expression was observed. On the basis of the obtained results, it was established that the investigated breeds are heterogeneous and the isoelectric focusing method is successful when specifying the inner-race and inter-race polymorphism in silkworm.

• Asian-Australasian Journal of Animal Sciences
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• 제33권11호
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• pp.1732-1740
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• 2020

Objective: The objectives of this study were to investigate the genetic diversity, population structure and relatedness among the five chicken populations of Bangladesh using microsatellite markers. Methods: A total of 161 individuals representing 5 chicken populations (non-descript Deshi [ND], naked neck [NN], hilly [HI], Aseel [AS], and red jungle fowl [JF]) were included in this study to investigate genetic diversity measures, population structure, genetic distance and phylogenetic relationships. Genotyping was performed using 16 selected polymorphic microsatellite markers distributed across 10 chromosomes. Results: The average observed and expected heterozygosity, mean number of alleles and polymorphic information content were found to be 0.67±0.01, 0.70±0.01, 10.7 and 0.748, respectively in the studied populations. The estimated overall fixation index across the loci (F), heterozygote deficiency within (F_IS) and among (F_IT) chicken populations were 0.04±0.02, 0.05 and 0.16, respectively. Analysis of molecular variance analysis revealed 88.07% of the total genetic diversity was accounted for within population variation and the rest 11.93% was incurred with population differentiation (F_ST). The highest pairwise genetic distance (0.154) was found between ND and AS while the lowest distance was between JF and AS (0.084). Structure analysis depicted that the studied samples can be categorized into four distinct types or varieties (ΔK = 3.74) such as ND, NN, and HI where AS and JF clustered together as an admixed population. The Neighbor-Joining phylogenetic tree and discriminant analysis of principal component also showed close relatedness among three chicken varieties namely AS, HI, and JF. Conclusion: The results reflected that indigenous chicken of Bangladesh still possess rich genetic diversity but weak differentiation among the studied populations. This finding provides some important insight on genetic diversity measures that could support the designing and implementing of future breeding plans for indigenous chickens of Bangladesh.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1519-1523
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• 2005

By screening a subtracted cDNA library constructed with mRNA obtained from the longissimus dorsi muscles of F1 hybrids Landrace${\times}$Yorkshire and their Yorkshire female parents, we isolated two partial sequences coding for the H3-K4-specific methyltransferase (KIAA1717) and skeletal muscle myosin regulatory light chain (HUMMLC2B) genes. In the present work we investigated two SNPs, one (C1354T) at the 3' untranslated region (UTR) of KIAA1717 and one (A345G) at the SINE (PRE-1) element of HUMMLC2B, in a resource population derived from crossing Chinese Meishan and Large White pig. The selected pigs were genotyped by means of a PCR-RFLP protocol. Significant associations were observed for the KIAA1717 C1354T polymorphic site with thorax-waist backfat thickness (p<0.05), buttock backfat thickness (p<0.05), average backfat thickness (p<0.05), loin eye height (p<0.05), loin eye area (p<0.05), carcass length to 1$^{st}$ spondyle (p<0.01) and carcass length to 1st rib (p<0.01). HUMMLC2B A345G were significantly associated with loin eye width (p<0.05), loin eye area (p<0.05). Further studies are needed to confirm these preliminary results.

• 한국패류학회지
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• 제28권4호
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• pp.343-347
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• 2012

속성장을 위한 선발 1세대 전복 (Selected abalone : SA)의 성장률을 조사하기 위하여 동일 사육 환경 조건에서 대조구 전복 (Control abalone : CA) 과 비교실험을 하였다. 그 결과 각 성장형질 (각장, 각폭 및 중량) 은 SA구가 CA구보다 유의하게 빠른 것으로 나타났다. 45일째 및 90일째의 평균 각장에 있어 SA구가 CA구 보다 각각 31.7% 및 17.8% 빠르게 나타났으며, 중량에 있어서도 각각 43.7% 및 25.6% 빠른 것으로 나타났다. 더욱이 90일째의 각장과 중량의 상대성장 비교에 있어서도 SA구가 CA구 보다 유의적으로 높은 상관관계를 나타내었다. 이러한 결과는 선발육종기술을 이용하여 양식 전복의 성장률을 증대할 수 있는 가능성을 시사하고 있다.