• 한국어병학회지
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• 제21권1호
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• pp.21-27
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• 2008

2006년　5월　전북　군산시에　위치한　미꾸라지　양식장에서　미꾸라지가 대량으로 폐사하였다.　병어는　궤양을　동반한　체표의　출혈반점과　아가미　빈혈　및　울혈　있었고　간의　퇴색과　비장, 신장의　종대　및　장의　출혈이　나타났다. 병어의 간, 비장, 신장에서　그람음성의　단간균이　검출되었고　생화학적　시험측정　결과　분리균은 Aeromonas sobria로　판명되었다. 분리균과 A. sobria가 소유한 aerolysine 유전자　일치　여부를 분석한 결과 같은　유전자를　가지고　있음이　판명되었다. 분리균을　미꾸라지에　농도별로　복강주사　한　결과　자연발생어의 증상과　일치하였다. 그럼으로 A. sobria는 양식　미꾸라지의 새로운 세균성　질병의 원인균이 될 가능성이 높다고 판단된다.

• 한국과학교육학회지
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• 제7권2호
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• pp.21-35
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• 1987

Polyurethane foam was utilized to collect protists from the 2 ponds in Seoul National University and another pond in vicinity of Paldang Dam. The identified protists were 4 species in Cyanophyta, 2 species in Euglenophyta, 19 species in Chrysophyta, 9 species in Chlorophyta, 2 species in Ciliophora, and 3 species in Sarcodian. To test the adoptability of the sampling method for classes in junior and senior high school, frequency of the 25 protists in each observation and in each preparation were calculated. The families which were present at high frequencies were Naviculaceae. Fragilariaceae, Cymbellaceae and Coelastraceae. In an experimental application of the 40 pictures taken from the samples, students in junior classes recognized the protists by their outer morphology, internal structure. color, and colonial types, in decreasing order. Use of the collecting method and the applicability of protists for teaching biology were discussed.

• 한국조경학회지
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• 제27권5호
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• pp.48-56
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• 2000

This study aims to find out the norms and principles reflected in the spatial organization of royal tombs in the Chosun Dynasty through the modern interpretation of traditional oriental philosophy theory. We examined the characteristics of royal tomb sites including Jeshil(Shrine), Hongjunmun, tomb mound, ponds, and attached buildings employing an analysis of the spatial formation and landscape. This study covered 36 tombs of kings and queens in the Chosun Dynasty. The entire layout of royal tombs, including annexed buildings, was informal in may cases. This was due to following the natural contour rather than adhering to certain philosophical principles. The royal tomb site is divided into three zones. Jeshil and pond constitute a space for living people, visitors. Secondly, the semi-scared space includes the space from Hongjunmun to the entrance to the Chungjaka. The last space is for the dead, from the back of Chungjakak to tomb mound. This is in line with the layout of palaces of the Chosun Dynasty which is characterized by "three gates and three zones": outer yard-court yard- back yard. The size of the space for holding memorial ceremonies, from Hongjunmun to the Chungjakak entrance, was set at 900m until the early Chosun Dynasty. Since that time the distance varied according to the land topography due to the influence of metaphysics. It can be summarized that the spatial organization of royal tombs in the Chosun Dynasty is based on the Confucianism value, the natural contour of the site, and inner spiritual value.ual value.

• 교육시설
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• 제8권4호
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• pp.5-15
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• 2001

The purpose of this study is to clarify the using condition and requirement for outdoor space between the parallel types of elementary school buildings focused on Chungbuk province in Korea. Consequently, we investigated the actual conditions of students and teachers, using conditions of outdoor space between school buildings. The summary of outdoor space can be given below. 1) In the using condition of outdoor spaces, most of them have path & covered walks, garden, playground and soon. The distances between neighboring building fixed, the use and quality of outdoor space is differently to spatial configuration of school buildings. For example, garden functions as itself in case of D/H>3, but the other space to observe in D/H>3. 2) In the requirements of outdoor spaces, the amount of scatter number of classmates and teachers is wide. The favoring outdoor space is the woods to take a rest by oneself, the demanding outdoor space is almost a resting place like a school store in case of classmates and the natural facilities like ponds garden pagora in teachers.

• Bulletin of the Korean Chemical Society
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• 제33권4호
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• pp.1183-1189
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• 2012

The KOrea Insecticide Exposure Model (KOIEM) was developed to facilitate ecological risk-based management of Korean insecticides. KOIEM, applied as a multimedia fate model, evaluates water, soil, air, and vegetation compartments based on three water-body types (streams, ditches, and ponds). Deltamethrin, a pyrethroid insecticide, was used to evaluate and create the model parameters. After exposure of both the stream and the ditch to deltamethrin, the KOIEM-predicted concentrations and the observed levels were in agreement. The model was also evaluated using the accuracy factor (AF), which was 4.32 and 0.35 for the stream and ditch, respectively. Ecological risk assessment was also performed to evaluate the application of KOIEM for four popular South Korean insecticides (cypermethrin, deltamethrin, diazinon, and permethrin). Despite the insecticides having low PECs in water, their risk quotients were typically above 1.0. Thus, KOIEM modification would be required in further studies to account for spatial variation.

• Journal of Microbiology
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• 제43권1호
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• pp.1-10
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• 2005

We isolated and cultured bacteria inhabiting solar saltern ponds in Taean-Gun, Chungnam Province, Korea. All of the isolated 64 strains were found to be moderately halophilic bacteria, growing in a salt range of 2-20 %, with an optimal concentration of 5% salt. Bacterial diversity among the isolated halophiles was evaluated via RFLP analyses of PCR-amplified 16S rDNAs, followed by phylogenetic analysis of the partial 16S rDNA sequences. The combination of restriction enzyme digestions with HaeIII, CfoI, MspI and RsaI generated 54 distinct patterns. A neighbor-joining tree of the partial 16S rDNA sequences resulted in the division of the 64 strains into 2 major groups, 45 strains of ${\gamma}-Proteobacteria$ (70.3%) and 19 strains of Firmicutes (29.7%). The ${\alpha}-Proteobacteria$ and Cytophaga-Flavobacterium-Bacterioides groups, which were repeatedly found to exist in thalassohaline environments, were not represented in our isolates. The ${\gamma}-Proteobacteria$ group consisted of several subgroups of the Vibrionaceae (37.5%), Pseudoalteromonadaceae (10.9%), Halomonadaceae (7.8%), Alteromonadaceae (7.8%), and Idiomarinaceae (6.3%). Members of Salinivibrio costicola (29.7%) were the most predominant species among all of the isolates, followed by Halobacillus treperi (12.5%). Additionally, three new species candidates were found, based on similarities of the 16S rDNA sequences to those of previously published species.

• 한국조경학회지
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• 제24권4호
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• pp.85-95
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• 1997

The purpose of this study is to investigate the function of "Chang-Won-Seo" which is one of government organizations in charge of landscape architecture during Chosun-Dynasty This study is based on the analysis of historic documents published by the government offices during Chosen-dynasty. The landscape architectural administration during Chosun-Dynasty was mainly undertaken by the Ministry of Industry in both central and local government. Especially, the government office titled "Seon-Gong-Gam" under the Ministry of Industry was in charge of landscape construction. There were seven divisions under the "Chang-Won-Seo" and three divisions such as "Kwa-Won-Saek" , "Saeng-Gwa-Saek" and "Keon-Gwa-Saek" took charge of main part of the work. "Kwa-Won-Saek" was In charge of the management of public fruit gardens and lotus ponds constructed in Han-Yang(Seoul) in order to harvest fruit and edible lotus seeds. "Saeng-Gwa-Saek" was responsible for the supply of fresh fruits to the royal family and the government offices while "Keon-Gwa-Saek" took charge of supplying them with dried fruits. In view of the responsibilities of three divisions, it is concluded that the function of "Chang-Won-Seo" was not to construct and manage the pleasure gardens controlled by the government. but to manage the public fruit gardens and to supply the royal family and the government offices with fresh and dried fruits.

• 한국환경복원기술학회지
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• 제13권6호
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• pp.107-115
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• 2010

This study was surveyed to identify changes of flora during three years after restoration in Cheonggyecheon stream. There are four sections in Cheonggyecheon. One and two sections are upper streams and three and four sections are down streams. It was surveyed 328 species in 2006. In 2007 and 2008, 446 and 444 species were found, respectively. This result shows that Cheonggyecheon is unstable initial condition in restored stream ecosystem. Naturalized species were 58 species in 2006 and it was respectively 61 and 63 species in 2007 and 2008. Hazard species of ecosystem were three common species during survey period. In appearance of flora per section, three and four sections constituted by natural sites such as point bars, wide flood plains, riffles and ponds, marshes, etc. were surveyed more species than one and two constructed by concrete materials and narrow flow channel. Recently, as time goes by, introduced species are being increased. And succession has mainly been progressed by one year or binary herbs and perennial herbs. Compared with other restored streams, Cheonggyecheon showed more flora than Yangjaecheon and Anyangcheon. It is judged owing to length of surveyed site, various planted species and area of inhabitation space. To manage restored stream ecosystem, monitoring is essential. Further, because change of vegetation after restoration in Cheonggyecheon is very important, continuous monitoring about Flora and Naturalized species and Hazard species of ecosystem is also very important.

• Journal of Microbiology and Biotechnology
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• 제16권10호
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• pp.1640-1645
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• 2006

We studied the diversity of the halophilic archaea and bacteria in crystallizer ponds of a Korean solar saltern by analyzing 16S rRNA gene libraries. Although diverse halophilic archaeal lineages were detected, the majority (56%) were affiliated with the uncultured and cultured Halorubrum group. Halophilic archaea that have been frequently observed in solar saltern environments previously, such as Halogeometricum, Halococcus, Haloarcula, and Haloferax, were not detected in our samples. The majority of clones (53%) belonged to the Cytophaga-Flavobacterium-Bacteroides and ${\alpha}-,\;{\gamma}-,\;and\;{\delta}-Proteobacteria$ groups, with 47% of the clones being affiliated with ${\gamma}-Proteobacteria$. We also identified new ${\delta}-Proteobacteria$-related bacteria that have not been observed in hypersaline environments previously. Our data show that the diversity of the halophilic archaea and bacteria in our Korean saltern differs from that of solar salterns found in other geographic locations. We also showed by quantitative real-time PCR analysis that bacteria can form a significant component of the microbial community in solar salterns.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1350-1358
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• 2012

Viral pathogens, alongside other pathogens, have major effects on crustacean aquaculture. Hepatopancreatic parvovirus (HPV) is an emerging virus in the shrimp industry and has been detected in shrimp farms worldwide. The HPV genome has greater diversity than other shrimp viruses owing to its wide host range and geographical distribution. Therefore, developing diagnostic tools is essential to detect even small copy numbers from the target region of native HPV isolates. We have developed two easy to use quantitative real-time PCR kits, called Green Star and Dual Star, which contain all of the necessary components for real-time PCR, including HPV primers, using the primers obtained from the sequences of HPV isolates from Korea, and analyzed their specificity, efficiency, and reproducibility. These two kits could detect from 1 to $1{\times}10^9$ copies of cloned HPV DNA. The minimum detection limits obtained from HPV-infected shrimp were $7.74{\times}10^1$ and $9.06{\times}10^1$ copies in the Green Star and Dual Star assay kits, respectively. These kits can be used for rapid, sensitive, and efficient screening for HPV isolates from Korea before the introduction of postlarval stages into culture ponds, thereby decreasing the incidence of early development of the disease.