• 대한한방내과학회지
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• 제21권1호
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• pp.156-161
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• 2000

The unripe fruit of Poncirus trifoliata Raf has been used for the treatment of allergic disease. Recently it was reported that the fruit inhibits passive cutaneous anaphylaxis and histamine release at mast cell. Type I immediate hypersensitivity of anaphylactic type is caused by released mediate chemical at mast cell. Histamine is also known as one of potent mediate chemical. Also release of mediate chemical is affected by specific stimulation of IgE combined with mast cell. Activation of mast cell is known to be stimulated by compound 48/80 and inhibited by increase of cAMP. In this experiment, the effect of water extract of Poncirus trifoliata Raf fruit (PT) on a histamine release, cAMP concentration and IgE production was measured. Compound 48/80 was administrated to the mouse peritoneal cell which was pretreated with PT. PT dosedependently inhibited histamine release at peritoneal mast cell and the serum level of histamine induced by compound 48/80. PT also instantly increased cAMP level of peritoneal mast cell right after it was added and the level gradually decreased. Production of IgE induced by antigens at mouse peritoneal cell was inhibited by PT. The IgE synthesis is induced by IL-4 and it is known that lipopolysaccharide(LPS) plus IL-4 cause an increase in IgE secretion by murine B cells. The effects of PT inhibited the production of IgE activated by LPS plus IL-4 at human U266B1 cells. These results indicate that PT has antiallergic activity by Inhibition of IgE production from B cells and histamine release by increase of cAMP.

• 대한본초학회지
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• 제27권5호
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• pp.9-14
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• 2012

Objective : We investigated the effect of Poncirus trifoliata and Citrus aurantium extract in mice with cerulein-induced acute pancreatitis (AP) model. Methods : AP was induced via intraperitoneal injection of cerulein (50 ${\mu}g/kg$) given every hour for 6h. Poncirus trifoliata (PT: 200 or 400 mg/kg) and Citrus aurantium (CA: 200 or 400 mg/kg) extract were injected 1 h before in mice with cerulein-induced AP. Mice were sacrificed at 6 h after last injection of cerulein. Blood samples were taken to determine serum amylase and lipase levels. The pancreas and lungs were rapidly removed for morphological examination, myeloperoxidase assay. Results : PT pre-treatment significantly protected the pancreas and lung damages and reduced the MPO activity and serum amylase in cerulein-induced AP. However, CA pre-treatment did not significantly protected the pancreas and lung inflammation in cerulein-induced AP. Conclusion : These results suggest that PT but not CA could protect the cerulein-induced AP. Conclusion : These results suggest that PT but not CA could protect the cerulein-induced AP.

• 한방재활의학과학회지
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• 제18권4호
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• pp.13-24
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• 2008

Objectives : The purpose of this study was to investigate the effect of Poncirus Trifoliata(PT) on improvement of fecal impaction in spinal cord injured(SCI) rats. Methods : Fifteen adult Sprague-Dawley female rats were used weighing 200~250 g. A complete spinal cord transection was performed surgically at the T10 cord level. Experimental groups were assigned into 3 groups: Control(n=5), SCI+vehicle(n=5) and SCI+PT(n=5). PT was administered 100mg/kg in 0.5ml every 24 hours from 1st operation day to 7th day. We measured the body weight and food intake as well as the number and the weight of fecal pellet every morning. After 1 week of operation, whole colon was divided into proximal and distal segments under anesthesia. Each segment of colon was mounted with longitudinal direction in a organ bath. We measured spontaneous contraction and compared the area under the curve in each segments. Enhanced responses were observed by acetylcholine($10^{-6}M$), 40 mM KCl solution, L-NAME($10^{-4}M$). Results : The fecal number and weights were significantly higher in the group of SCI+PT than SCI+vehicle group(p<0.05). In organ bath study, area under the curves of the spontaneous contraction in SCI+vehicle and SCI+PT groups were significantly increased compared to control group. Contractility of distal colon in response to acetylcholine or KCl in SCI+vehicle group was significantly decreased compared to other groups(p<0.05). Conclusions : These results suggest that PT might be useful to promote bowel emptying in spinal cord injured rats.

• 한국미생물·생명공학회지
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• 제30권2호
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• pp.177-183
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• 2002

40여종의 한약재추출물을 제조하여 가금질병에 관련이 있는 세균에 대한 항균활성을 조사하였다. 오미자, 천련자, 소목 및 오배자 추출물은 Salmonella gallinarum에 대해 높은 항균활성을 보였으며 향유, 육두구, 초두구, 지실, 하고초, 소목 및 오배자 추출물은 Staphylococcus aureus에 대해 높은 항균활성을 보였다. 천련자, 소목 및 오배자 추출물의 S. gallinarum에 대한 최소저해농도는 1.2 ㎎/ml이며 오배자 추출물의 S. aureus에 대한 최소저해농도는 0.6 mg/ml로 가장 낮은 농도에서 항균활성을 보였다. 열에 의한 항균활성 변화에 있어서 오미자와 오배자 추출물은 S. gallinarum에 대한 항균럭이 감소하였고 초두구와 소목 추출물은 S. aureus 에 대해 항균력이 안정하였다. pH에 의한 항균활성의 변화에서 S. gallinarum에서 오미자가, S. aureus에서는 향유, 지실 및 오배자는 산성역에서는 비교적 안정하였으나 알칼리역에서 불안정하였으며 소목은 산성과 알칼리역에서 안정하였다. 항균력이 가장 우수한 오배자와 소목 추출물의 미생물 증식억제 효과를 조사하기 위해 증식배지에 0, 100, 300 및 500 ppm의 추출물을 첨가하여 균주의 증식 억제효과를 조사하였다. 배양후 24시간에 무첨가구에 비해 100 ppm이상의 오배자 추출물 첨가시 시험 균의 증식을 현저히 억제하였고, 소목 추출물에서도 무첨가구보다 300 ppm 이상의 추출물 첨가시 시험 균의 증식을 현저히 억제하였다. 이런 결과를 종합하면 가금 질병을 유발하는 S. gallinarum과 S. aureus를 예방 할 수 있는 사료첨가제 개발 가능성을 확인하였다.