• Journal of the Korean Society of Food Science and Nutrition
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• v.26 no.3
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• pp.404-410
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• 1997

Amylase, protease, polygalacturonase and $\beta$-galactosidase activities were monitored during salting of Chinese cabbage and kimchi fermentation at 1$0^{\circ}C$. A part of enzymes in the tissue of Chinese cabbage were eluted during salting, and other remained enzymes activities were decreased in proportion to the amount of elution. But total enzyme activities were increased during salting. Amylase, protease and polygalacturonase activities decreased at the early fermentation stage but increased at the late fermentation stage. $\beta$-Galactosidase activity was continuously increased during all periods of fermentation. Enzymic actions at the early fermentation stage come from Chinese cabbge and at the late fermentation stage come from major microorganisms in kimchi fermentation. Kimchi fermentation involves the activation of the enzymes by salting; hydrolysis of micromoleculars such as polysaccarides cell wall composed polysaccarides and proteins of cell wall during early fermentation of kimchi; overripening of the kimchi caused by propagation of homofermentative lactic acid bacteria which demand autotroph.

• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.1
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• pp.121-126
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• 2009

Korean ginseng (Panax ginseng C. A. Meyer) has been used as an important medicinal plant in the Orient for a long time. It has been claimed that ginseng has many beneficial bioactive effects on human health, such as antitumor, antistress, antiaging and enhancing immune functions. Red ginseng possibly have new ingredients converted during steaming and dry process from fresh ginseng. In this study, pharmacological efficacy and ingredient conversion of ginseng by 9 repetitive steaming and drying process were investigated measuring conversion efficiency of acidic-polysaccharide, phenolic compounds and inhibition of peroxide lipides. It was found that acidic-polysaccarides were increased by heat treatment. In addition, maltol of phenolic compounds, strong antioxidant, produced during the process of red ginseng by Maillard reaction. Acidic-polysaccarides and maltol were increased after the 1st and 3rd steaming and drying treatments, but they were decreased gradually after 5th, 7th, and 9th treatments. Antioxidant activity was increased as increasing treatment times of steaming and drying without significance. Effect of red ginseng extract on inhibition of peroxide was increased gradually until after the 7th treatment, but remarkably decreased after the 9th treatment.

• Microbiology and Biotechnology Letters
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• v.21 no.5
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• pp.478-485
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• 1993

The strain which produced highly viscous exopolysaccharides (EPS) in liquid culture was selected from soil. The strain was supposed to Bacillus sp. from the results of mophological, biochemical and physiological tests. The medium composition for EPS production was trypton 0.75%, sucrose 4%, CaCO3 0.01%, Winogradsky's nitrogen free mineral medium 5ml/l and pH 7.0. In 2-l jar fernenter, the viscosity of culture broth after 120-hr cultivation time was very high (60, 000 cps) and the amount of EPS was 6.2g/l.

• Applied Chemistry for Engineering
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• v.21 no.2
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• pp.154-161
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• 2010

In the study, the effect of acid and salt concentrations during the production of bio-ethanol from various brwon-algae raw materials was investigated. Especially, the possibility of the conversion of various polysaccarides contained in Laminaria japonica was studied. Bio-ethanol was produced by Saccharomyces cerevisiae KCCM1129 strains in Laminaria japonica. The maximum bio-ethanol production of 2.09 g/L using heat-treatment of Laminaria japonica was achieved. The optimum concentration for reducing sugar conversion by Laminaria japonica was found to be 3.95 g/L at the HCl concentration of 0.1 N. But bio-ethanol production was higher than the case without the non-acid pretreatment. Among the various polysaccharides, only mannitol produced maximum 3.09 g/L bio-ethanol. In case of laminaran, the ethanol was produced only at 0.15 g/L only in 0.1 N HCl pretreatment medium and cell growth was higher than other pretreatment.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.105-110
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• 2000

Various environmental factors such as pH, temperature and initial glucose concentration were investigated for enhancing cell growth in fermentations of Phellinus linteus WI-OOl, a producer of polysaccarides with potent anticancer activities. Optimal pH and temperature were around 5.5 and $28^{\circ}C$, respectively. Relatively little variation of pH was observed ranging between 5.5 and 6.5 during the whole fermentation period. Maximum cell concentration and specific growth rate were investigated in the media containing initial glucose concentrations of 0.5%, 1 %, 2%, 3% and 4%. High initial glucose concentration enhanced biomass production but showed negative effect on specific growth rate. In bioreactor experiments with various feeding strategies, increases of 28% and 42% in final cell concentration were obtaind as compared to conventional batch process, by adopting pulse and continuous supplement of 2% glucose solution, respectively.

• Korean Journal of Plant Resources
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• v.9 no.3
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• pp.218-223
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• 1996

This study was carried out to obtain a basic information for the improvement of human health and the development of variety through the analysis of inorganic compounds. contents of amino acids and polysaccarides on three elephant foods, Amorphophallus konjac, collected from Kumsan, Japan and China. The contents of mannan as a carbohydrate of a major component in an elephant food was slightly high in Kumsan than that of Japan and China variety. Degree of VIscosity of an elephant food depends upon the contents of soluble free sugars and amino acids contents of these free sugars were high in the order of Japan, China and Kumsan variety. The analytical results on inorganic compounds in an elephant food were shown as follows; The contents of potasium, phosphate and ferrin of Kumsan variety were shown to be higher than those of China and Japan, while sodium and caleium were appeared to be highter in China chip

• Korean Journal of Food Science and Technology
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• v.34 no.5
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• pp.757-761
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• 2002

Proximate composition, phenolic compounds, and amino acid composition of molokhia (Corchorus olitorius) and molecular weight distribution of its mucilage were determined. Concentrations of dietary fibers and protein were 37.4 and 24.4% of the total dry weight, respectively. Mucilage was obtained by extracting dry molokhia leaves with hot water, followed by the addition of 55% ethanol. Although about 50% of the mucilage was lost during the extration, it was still rich in polyphenol compounds. In mucilage, proteins were composed of glutamic acid, glycine, alanin, and aspartic acid, and molecular weight of most polysaccarides ranged from $40,000{\sim}500,000$ daltons based on gel filtration chromatography.

• Korean Journal of Microbiology
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• v.43 no.4
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• pp.292-297
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• 2007

This study was performed to compare in vitro immune enhancing effects of polysaccharides extracted from cultivated mycelia of Agaricus blazei Murill. Carbohydrate contents of semi-purified polysaccharides were 85.6% and 95.3%, while ${\beta}$-glucan conents were 67.9% and 88.1% for intracellular and extracellular polysaccharide, respectively. Samples were adjusted to the same in their carbohydrate contents before efficacy tests. Both intracellular and extracellular polysaccharide increased nitric oxide (NO) synthesis of macrophage RAW 264.7 in dose dependent manner, and the maximum increase rate was 53.9 and 53.1% in intracellular and extraceltular polysaccharide, respectively. The polysaccharides also increased synthesis of cytokines such as interleukin (IL)-$1{\beta}$, IL-6 and tumor necrosis factor (TNF)-${\alpha}$ in RAW 264.7. For all the 3 cytokines, the increase rate of synthesis was much higher in extracellular polysaccharide compared to intracellular polysaccharide, especially at low concentration. Both polysaccarides increased the proliferation of splenocytes in vitro, intracellular polysaccharide showed increase in dose dependent manner while extraceltular polysaccharide showed increase untill medium concentration ($250\;{\mu}g/ml$). They did not show direct cytotoxicity against cancer cells such as B16F0 melanoma. As results, it was regarded that the both intracellular and extracellular polysaccharide from A. blazei showed immune enhancing effects in vitro, but the activity is higher in extracellular polysaccharide compared to intracellular polysaccharide.

• Korean Journal of Food Science and Technology
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• v.25 no.6
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• pp.672-676
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• 1993

Rheological properties of the aqueous solution of various polysaccarides and their effects as a stabilizer in fruit(peach) syrup and yogurt were investigated. Four kinds of modified starch(Sun Kolguard, Sun Registar, Mira Cleer 340 and Maps 449), three types of carrageenan(kappa, iota and lambda types) and pectin were used in this study. The 5% aqueous solution of each modified starch was gelatinized at $66^{\circ}C$. After gelatinization, Sun Kolguard showed the highest viscosity among those samples. The synergistic effect of carrageenan on 5% aqueous solution of Sun Kolguard was studied by changing the mixing ratio, and the result showed that the ratio 90:10(Sun Kolguard:carageenan) had the maximum synergistic effect and the effect of iota type carrageenan was higher than that of other types. The effect of Sun Kolguard as a stabilizer in fruit syrup were superior to other modified starches studied. The gelatinization temperature of the addition of iota carrageenan in starch added fruit syrup changed from $66^{\circ}C$ of 5% aqueous solution to $82^{\circ}C$. Furthermore, the viscosity after cooling of syrup which made iota carrageenan and starch added fruit syrup was more increased than simple starch added fruit syrup. The mixture of 90% Sun Kolguard and 10% iota carrageenan were estimated as the most appropriate stabilizer for manufacturing the fruit syrup and yogurt adding fruit syrup.

• Journal of Nutrition and Health
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• v.1 no.3_4
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• pp.201-205
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• 1968

The authors has observed the histopathologically and histochemically on the effect of the garlic and garlic oil to the liver and kidney of rats. In order to confirm the histochemical changes of the metabolism of polysaccarides, the periodic acid Schiff reaction was applied. The 30 albino adult male rats weighing about 150 grames from the National Institute of Health were housed individualy and devide into 3 experimental groups: Group C: stock diet group Group B: stock diet-garlic group Group A: stock diet-garlic oil group Group C was fed with stock diet only through out this experimental period, Group B was fed with stock diet supplemented with garlic homogenator to be 1%, and Group A was fed with stock diet supplemented with the garlic oil to 0.05%. The garlic oil used in this experiments was extracted by author. And all rats was fed during 10 weeks. The histopathological and histochemical results were shown in each figure. According to the all results, the following concIusions were drawn. 1) In the garlic oil administrated groups, congestion of the sinusoid was subsided and the liberation of the Kupffer's cells were observed. 2) In garlic administrated groups, fatty metamorphosis in hepatic cells, and slight liberation of Kupffer's cells in sinusoidal walls were observed. Connective tissue proliferation and collagen bundle were observed. 3) The connective tissue and blood vessel wall in portal area Were reacted intensely with PAS stain. The hepatic cells Were reacted intensely with PAS stain in control group and moderately or slightly in garlic and garlic oil administrated group. 4) There were no significant differences in collecting and Henle's loops in each groups, but narrowing of lumen of the distal tubules were observed in garlic oil administrated group. 5) The basement membrane of the tubules and the connective tissues of the vessel wall in Kideny were reacted intensely with PAS stain in each groups. In control and garlic administrated groups. the brush border of the proximal tubules were reacted intensely with PAS stain, but epithelium of the Heine's loop, proximal, distal and collecting tubules were reacted moderately. In garlic oil administrated-group, there were tendency of decreasing of PAS stain in each tubules.