• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.63-63
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• 2003

In an effort to uncover the spermatogenic impairment by the polychlorinated biphenyls (PCBs), the expression of tight junctions (TJs) genes important for the formation of the blood testis barrier (BTB) were examined following the 3,3',4,4',5-pentachloro biphenyl (PCB126) treatment in cultured neonatal testis in mice. At 4 days (D4) after 10 and 100 nM PCB126 treatment the expression of claudin-11 was significantly increased when compared with vehicle control. In contrast no difference in occludin and claudin-1 expression was found among the experimental group. On D8, 100 nM PCB126 significantly increased the expression of claudin-11 but not occludin and claudin-1. 1 uM PCB126 treatment significantly decreased expressions of occludin and ciaudin -1, suggesting the general toxic effect on the Sertoli cell. Because PCB126 does not alter the proliferative activity of spermatogenic cells and Sertoli cells in neonatal testis, it is likely that increase in the expression of claudin-11 by low dose of PCB126 may attribute to the alteration of the Sertoli cells differentiation in testis. It also emphasized that PCB126 might have differentially affected the transcription of TJ genes in Sertoli cells. In conclusion, this result suggests that the structure of TJ may be targeted by PCB126 in neonatal testis in mice and that co-PCB is potentially harmful to spermatogenesis by alteration of the development of BTB.

• Journal of Microbiology and Biotechnology
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• 제9권6호
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• pp.804-810
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• 1999

There is a possibility that carvone, a monoterpene from spearmint (Mentha spicata), could induce the bph degradative pathway and genes in Alcaligenes eutrophus H850, which is a known Gram-negative PCB degrader with a broad substrate specificity that was thoroughly investigated with Arthrobacter sp. BIB, a Gram-positive PCB degrader. The strains BIB and H850 were unable to utilize and grow on the plant terpene [(R)-(-)-carvone] (50ppm) to be recognized as a sole carbon source. Nevertheless, the carvone did induce 2,3-dihydroxybiphenyl 1,2-dioxygenase (encoded by bphC) in the strain B lB, as observed by a resting cell assay that monitors accumulation of a yellow meta ring fission product from 4,4'-dichlorobiphenyl (DCBp). The monoterpene, however, did not appear to induce the meta cleavage pathway in the strain H850. Instead, an assumption was made that the strain might be using an alternative pathway, probably the ortho-cleavage pathway. A reverse transcription (RT)-PCR system, utilizing primers designed from a conserved region of the bphC gene of Arthrobacter sp. M5, was employed to verify the occurrence of the alternative pathway. A successful amplification (182bp) of mRNA transcribed from the N-terminal region of the bphC gene was accomplished in H850 cells induced by carvone (50ppm) as well as in biphenyl-growth cells. It is, therefore, likely that H850 possesses a specific PCB degradation pathway and hence a different substrate specificity compared with B1B. This study will contribute to an elucidation of the dynamic aspects of PCB bioremediation in terms of roles played by PCB degraders and plant terpenes as natural inducer substrates that are ubiquitous and environmentally compatible.

• 한국환경농학회지
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• 제14권3호
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• pp.272-281
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• 1995

낙동강의 수질에 큰 영향을 미치는 금호강 하류 하상에서의 저니토 중 PCBs 잔류량을 조사할 필요가 있다. 따라서 금호강과 낙동강 합류점으로부터 7.5km까지의 금호강 하상저니토 중 PCBs의 잔류량을 평가하여 다음과 같은 결과를 얻었다. 첫째, PCBs가 염소화되어 DCB로 전환되었으며, DCB의 최소검출량은 2pg이었고, 저니토 중 PCBs의 최저 검출농도는 0.5ng/g이었다. 둘째, 시료 100점에 대한 PCBs의 검출빈도는 79%였다. 전체 시료에서 50ng/g이하인 시료가 49%였으며 400ng/g이상인 시료가 9%였다. 셋째, PCBs농도는 금호강과 낙동강의 합류점으로부터 7.5km까지의 거리에서는 큰 변화가 없었다. 넷째, $0{\sim}250cm$까지의 저니토 깊이에 따른 PCBs분포에서, $50{\sim}100cm$ 깊이에서의 PCBs농도는 평균농도보다 높았다. 다섯째, 저니토 중 COD의 평균농도는 $14.5{\pm}11.4mg/g$였고, Org.C의 평균농도는 $3.41{\pm}3.55%$였다. 여섯째, 점토 및 유기물 함량이 낮은 모래 토양의 PCBs 농도는 평균농도의 115%로 높았다. 그러나 점토 및 유기물 함량이 높은 저니토에서의 PCBs농도는 평균농도의 69%로 낮았다. 일곱째, 시료 100점 에 대한 PCBs의 평균농도는 $131{\pm}156ng/g$이었다. 이는 일본 환경청의 PCBs에 대한 하상제거기준 10000ng/g보다 매우 낮았다.

• The Korean Journal of Physiology and Pharmacology
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• 제9권1호
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• pp.9-15
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• 2005

We attempted to analyze the mechanism of polychlorinated biphenyl (PCB)-induced neurotoxicity and identify the target molecules in the neuronal cells for PCBs.Since the developing neuron is particularly sensitive to PCB-induced neurotoxicity, we isolated cerebellar granule cells derived from 7-day old Sprague Dawley (SD) rats and grew cells in culture for additional 7 days to mimic PND-14 conditions. Only non-coplanar PCBs at a high dose showed a significant increase of total protein kinase C (PKC) activity at phobol 12,13-dibutyrate ([$^3M$]PDBu) binding assay, indicating that non-coplanar PCBs are more neuroactive than coplanar PCBs in neuronal cells. PKC isozymes were immunoblotted with the selected monoclonal antibodies. PKC-${\alpha}$, ${\delta}$, and ε were activated with non-coplanar PCB exposure. Receptor for activated C kinase-1 (RACK-1), anchoring protein for activated PKC, was more induced with exposure to coplanar PCBs than non-coplanar PCBs. Reverse transcription PCR (RT-PCR) analysis showed induction of neurogranin (RC-3) and growth associated protein-43 (GAP-43) mRNA with non-coplanar PCBs. The results indicate that these factors may be useful biomarkers for differentiating non-coplanar PCBs from coplanar PCBs. The present study demonstrated that non-coplanar PCBs are more neuroactive congeners than coplanar PCBs.

• 한국환경과학회지
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• 제18권9호
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• pp.953-959
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• 2009

A practical and efficient disposal method for chemical dechlorination of PCBs (polychlorinated biphenyls) in transformer oil was evaluated. The transformer oil containing PCBs was treated by the PEG 600 (polyethylene glycol 600) and potassium hydroxide (KOH) along with different reaction temperatures(25, 50, 100 and $150^{\circ}C$) and times(30, 60, 240 and 480 min). The best disposal efficiency of PCBs in transformer oil was attained under the experimental conditions of PEG 600 (2.5 w/w%)/KOH (2.5 w/w%)/$150^{\circ}C$/4 hrs, showing completely removal of all PCBs containing 3-9 chlorines on two rings of biphenyl. In studying the reaction of PEG/KOH with PCBs, it confirmed that the process led to less chlorinated PCBs through a stepwise process with the successive elimination of chlorines.

• 분석과학
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• 제15권3호
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• pp.287-299
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• 2002

인천연안지역 표층퇴적물의 PCBs 잔류농도는 우리 나라의 다른 연안지역 보다 비교적 낮은 농도로 검출되었고, 전지역에 걸쳐 Aroclors 혼합표준액 (1016+1232+1248+1260)과 유사한 패턴으로 배출되었으며, 염소조성비율 변화에 따라 A(Kanghwa), B(Yellow) 및 C(Jangdo) 등 3개의 지역군으로 분류되었다. 또한, 외해에 근접한 B 지역은 2, 3 염화물이, 매립지 침출수 처리수가 방류되는 C지역은 7, 8염화물이 다른 지역에 비해 높은 염소 조성비율을 나타내는 것으로 조사되었다. 연구대상지역 주상퇴적물의 PCBs 잔류농도는 강화수로에서 1.60~3.00 ng/g, 장도수문에서 0.34~9.20 ng/g으로 나타났으며, 두 지역 모두 하부층에서 상부층으로 올라갈수록 농도가 증가하는 경향을 나타내었다.

• Fisheries and Aquatic Sciences
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• 제16권3호
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• pp.187-194
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• 2013

Microalgae are sensitive indicators of environmental changes, and hence they are widely used in environmental risk assessments and for the development of discharge guidelines. Here we evaluated the toxicity of metals and endocrine-disrupting chemicals (EDCs) to the marine green microalga, Tetraselmis suecica. The toxicants investigated included the metals, Cu, Ni, and Pb; and the EDCs, bisphenol A (BPA), endosulfan (ES), and polychlorinated biphenyl (PCB). The endpoints were variations in cell counts and chlorophyll a levels. T. suecica displayed a varied pattern of sensitivity to the toxicants. Based on the 72-h median effective concentration ($EC_{50}$), ES (0.045 mg/L) was most toxic to T. suecica, followed by PCB (3.96 mg/L) and Pb (9.62 mg/L). Interestingly, T. suecica was relatively tolerant to Cu (43.03 mg/L). The 72-h $EC_{50}$ values of Ni and BPA were approximately 16 mg/L. Our data suggest that this species may be relatively tolerant to most of the chemicals within their permissible limits in the environment.

• 한국해양바이오학회지
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• 제8권1호
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• pp.10-17
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• 2016

A wide-spread contamination of persistent organic pollutants (POPs) such as dioxins, PCBs, PBDEs in the aquatic ecosystem has generated a great concern over the potential risk for these substances to impact marine biotas and food web. Since a major exposure route of these substances to the humans is through the consumption of food including fish and marine byproducts, the consumption of contaminated products has been a great public health concern. Exposure to POPs has been associated with a wide spectrum of adverse effects including reproductive, developmental, immunologic, carcinogenic, and neurotoxic effects. This review covers the background information of key POPs substances and the recent development of toxicity studies including the mode of action. Because neurotoxic effects of some POPs have been observed in humans at low concentrations, polychlorinated biphenyl (PCB), a representative chemical of POPs, is focused to discuss the possible mode(s) of action for the neurotoxic effects. This review provides the updates of toxicity studies on POPs and paves ways to discuss a possible implication of contaminated marine biota over the human health among the marine biotechnology researchers.

• Journal of Microbiology
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• 제41권3호
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• pp.189-195
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• 2003

To enhance the reductive dechlorination of polychlorinated biphenyls (PCBs) under anaerobic conditions, we examined the adjunctive effects of cobalt (Co) and nickel (Ni), which are the central metals of transition-metal cofactors of coenzyme F$\_$430/ and vitamin B$\_$12/, respectively, on the dechlorination of Aroclor 1248. After 32 weeks of incubation, the average numbers of chlorines per biphenyl in culture vials supplemented with 0.2, 0.5, and 1.0 mM of Co reduced from 3.88 to 3.39, 2.92, and 3.28, respectively. However, the numbers of chlorine after supplementing with Ni decreased from 3.88 to 3.43, regardless of the Ni concentrations. The observed congener distribution patterns of all vials with different conditions were similar to the pattern produced by the dechlorination process of H' after 21 weeks of incubation, and these patterns were unchanged up to week 32, except for vials supplemented with 0.5 and 1.0 mM of Co. In vials containing 0.5 mM of Co, meta-rich congeners, such as 25/ 25-,24/25-, and 25/23-chlorobiphenyls (CBPs), which were found as accumulated products of dechlorination in other conditions, were further dechlorinated, and 25/2-, 24/2-, and 2/2-CBPs were concomitantly increased after 32 weeks of incubation. In this case, the congener distribution was similar to the dechlorination pattern of process M. From these results, we suggested that the enrichment of cultures with Co might stimulate the growth of specific populations of meta-dechlorinators, and that populations might promote a change in the dechlorination process from H' to M, which is known to be less effective on the dechlorination of the more highly chlorinated congeners of PCBs.

• 한국수산과학회지
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• 제30권4호
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• pp.679-686
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• 1997

본 연구조사는 동해남부 대마난류계에서의 PCBs의 분포특성을 파악하기 위하여 1996년 8월과 10월에 걸쳐 5개 정점을 선정하여 각 정점의 수심별의 수온, 염분 및 용존산소를 측정하여 수괴 분석을 실시하였으며, 또한, 각 정점별, 수심별의 PCBs의 분석을 실시하였다. 수괴의 분포특성을 조사한 결과 수온 및 염분약층은 수심 30m부분에 존재하고 있었으며, 대마난류 중층수는 30m이하로 간주되었으며, 대마난류의 표층수는 한국근해에서 대마도쪽으로 확장되는 양상을 보였다. PCBs의 분석결과 표층수는 8월의 경우 대마도쪽으로 갈수록 농도가 약간 낮아지는 경향을 보이다가, 대마도쪽에서 다시 약간의 증가경향이 관찰되었다. 표층 해수중에서의 PCBs의 평균농도는 $0.29\;ng/\ell$정도 였으며, 중층에서의 평균농도는 $0.31\;ng/\ell$정도로 표층에 비교하여 중층에서의 농도가 약간 증가하는 것으로 나타났다. 해수중 PCBs의 농도는 부유물질의 농도변화 경향과 유사성을 나타났으며, 농도의 수평 및 연직변화는 그다지 크게 나타나지 않아 비교적 균일한 농도분포를 나타내고 있었다. 해수의 수심별 PCBs의 상대농도 조성비는 표층에서 Di- 및 Tri-PCBs의 비율이 높았지만, 심층으로 갈수록 Peta-PCBs의 상대농도 조성비율이 높은 것으로 나타나, 수심의 변화에 따른 PCBs의 구성비와 소실에 차이가 있는 것으로 사료된다.