• 한국응용곤충학회지
• /
• 제41권4호
• /
• pp.269-277
• /
• 2002

곤충병원성 선충의 대상 해충에 대한 방제효과를 증대시키고 대량생산을 위한 기초자료를 얻기 위하여 온도와 접종농도가 Steinernema carpocapsae Pocheon계통의 꿀벌부채명나방(Calleriamellonella)유충의 감염력과 증식에 미치는 영향 및 노출시간과 토양깊이가 감염력에 미치는 영향을 조사하였다. 실험은 13, 18, 24, 30, $35^{\circ}C$의 온도조건과 꿀벌부채명나방 유충 한 마리당 0, 5, 10, 20, 40, 80, 160마리의 접종농도에서 수행하였다 온도와 접종농도는 모두 S. carpocapsae Pocheon 계통의 꿀벌부채명나방에 대한 감염성과 증식에 영향을 미쳤는데 $24^{\circ}C$에서 가장 좋았고, 온도와 접종농도가 증가할수록 치사시간은 단축되는 경향이었다 그리고 S. carpocapsae Pocheon계통은 모든 실험온도에서 꿀벌부채명나방 유충을 치사시켰지만 $13^{\circ}C$와 $35^{\circ}C$에서 발육은 하지 못하였다. S. carpocapsae Pocheon계통이 꿀벌부채명나방 유충 체내에서 증식되어 최초로 탈출하는데 소요되는 기간은 $18^{\circ}C$에서 20일 내외로 가장 길었고, $24^{\circ}C$나 $30^{\circ}C$에서는 5일 내외로 짧았다. S. carpocapsae Pocheon계통의 증식수는$ 24^{\circ}C$ 80마리 농도에서 꿀벌부채명나방 유충 1마리 당 18.8$\times$$10^4$마리로 가장 많았다. S. carpocapsae Pocheon 계통은 꿀벌부채명나방 유충에 300마리 농도로 접종하였을 때 10분만에도 침입하였다. 한편 S. carpocapsae Pocheon계통은 모래층의 깊이(0, 2, 5, l0 cm)에 상관없이 $10^{9}$마리/ha농도로 처리하였을 때 꿀벌부채명나방 유충에 대하여 100%의 치사율을 보였고, 토양 깊이별 선충의 성비도 차이가 없었으나 정착한 선충의 수는 깊이가 깊을수록 적었다. 따라서 S. carpocapsae Pocheon계통을 이용한 해충방제와 증식은 $24^{\circ}C$내외가 적당할 것으로 보이며 토양에서의 처리는 5cm이내에 서식하는 해충을 대상으로 하는 것이 바람직 할 것으로 보인다.

• Clinical and Experimental Reproductive Medicine
• /
• 제28권3호
• /
• pp.247-253
• /
• 2001

Objective: To analyze the methylenetetrahydrofolate reductase (MTHFR) mutation in patients with recurrent spontaneous abortion. Material and Method: The blood samples of patients with recurrent spontaneous abortion were tested by PCR-RFLP method. Results: Of 51 cases of study group, 14 (27.5%) were normal, 25 (49.0%) were heterozygosity, and 12 (23.5%) were homozygosity. Of 58 cases of control group, 20 (34.5%) were normal, 30 (51.7%) were heterozygosity, and 8 (13.8%) were homozygosity. But the difference between two groups was not significant (p=0.190). Conclusion: Hyperhomocysteinemia due to MTHFR mutation is a cause of recurrent spontaneous abortion. Therefore, the study for MTHFR mutation should be included in the workup of recurrent spontaneous abortion.

• Clinical and Experimental Reproductive Medicine
• /
• 제28권1호
• /
• pp.73-77
• /
• 2001

Objective: To report the pregnancy which was made by in vitro fertilization using recombinant follicle stimulating hormone and gonadotropin releasing hormone antagonist. Material and Method: Case report. Results: Six oocytes were retrieved and all were fertilized by intracytoplasmic sperm injection. Six embryos were transferred and the pregnancy was confirmed. Conclusion: It is envisaged that the availability of recombinant gonadotropins and gonadotropin releasing hormone antagonists will ultimately lead to shorter, cheaper and safer treatments, using reduced dosages.

• Clinical and Experimental Reproductive Medicine
• /
• 제26권3호
• /
• pp.497-501
• /
• 1999

Objective: To report the efficacy of vaginally administered bromocriptine. Material and Method: Case report. Results: The prolactin level was significantly decreased after the administration of bromocriptine vaginally. Conclusions: The vaginal administration of bromocriptine can be an alternative to oral administration in patients with hyperprolactinemia who show severe side effects.

• Clinical and Experimental Reproductive Medicine
• /
• 제28권3호
• /
• pp.209-213
• /
• 2001

Objective : To evaluate factor XII deficiency in patients with recurrent spontaneous abortion and its relation to aPTT. Material and Method: Factor XII was analyzed by clotting method. Results: Of 70 patients with recurrent spontaneous abortion, there were 35 cases of factor XII deficiency. Among them, there were only 3 cases of prolonged aPTT. Conclusions: It is still unclear whether factor XII deficiency is related to recurrent spontaneous abortion. Molecular approaches should be used to understand further the causal relationship. But based on this result, in the workup of patients with recurrent spontaneous abortion, factor XII should be included. aPTT is not likely to represent the abnormality of factor XII.

• Clinical and Experimental Reproductive Medicine
• /
• 제26권2호
• /
• pp.293-296
• /
• 1999

Different Y mutation in Yq11 occurring de novo in sterile males were first described 19 years ago. Since the phenotype of the patients was always associated with azoospermia or severe oligospermia, it was postulated that these mutations interrupt a Y spermatogenesis locus in the euchromatic Y region (Yq11) called azoospermia factor (AZF). Recently, it became possible to map AZF mutations to different subregions in Yq11by molecular deletion mapping. This indicated that azoospermia is possibly caused by more than one Y gene in Yq11 and the Yq11 chromatin structure. The frequency of AZF mutations in idiopathic sterile males $(5{\sim}20%)$ may indicate a need for a general screening programme for its analysis in infertility clinic. We have experienced a case of deletion distal to Yq11 region in azoospermic patient. So we report this case with a brief review of literatures.

• Clinical and Experimental Reproductive Medicine
• /
• 제26권3호
• /
• pp.491-495
• /
• 1999

Objective: To report the pedigree of Kallmann syndrome inherited in autosomal dominant mode with variable expressivity. Material and Method: Case report. Results: The patient had amenorrhea and anosmia but did not have a sign of absolute hypo gonadotropic hypogonadism. Her father had an anosmia and her two elderly sisters also had an anosmia but delivered babies uneventfully. Her two male siblings did not show any signs of hypogonadotropic hypogonadism. Conclusion: Kallmann syndrome has many different modes of inheritance such as autosomal dominant, autosomal recessive, and X-linked form. So the careful investigation of family pedigree is required.

• Clinical and Experimental Reproductive Medicine
• /
• 제27권3호
• /
• pp.307-311
• /
• 2000

Objective: To report the successful delivery in a patient of recurrent spontaneous abortion caused by chromosomal abnormality. Material and Method: Case report. Results: Twelve oocytes were obtained by in vitro fertilization. Of eleven oocytes fertilized, two embryos turned out to be normal by using fluorescent in situ hybridization on blastomere biopsy. The patient succeeded in pregnancy and the result of amniocentesis was found to be normal. She delivered the healthy female baby by cesarean section. Conclusions: The successful delivery is possible in recurrent spontaneous abortion related with reciprocal translocation by using preimplantation genetic diagnosis.

• 한국치위생학회지
• /
• 제11권1호
• /
• pp.113-126
• /
• 2011

Objectives : We supposed that identifying the influencing factors on DMFT index in the primary school children in Pocheon be indispensible for the primary school children's oral health program establishment in Pocheon. So, to prepare the basic materials for the primary school children's oral health program establishment in Pocheon, we analyzed the associated factors which could have an influence on the primary school children's DMFT index in Pocheon. Methods : We selected 3,676 primary school children in Pocheon, whose age were from 6 to 11, and surveyed those children by a questionnaire and the oral examination based on WHO's oral health survey method. Data were analyzed with a $x^2$-test and multiple regression analysis using SPSS $15.0^{(R)}$. Results : The results of oral survey in the primary school children in Pocheon revealed as follows: DMFT index, National Health Oral health survey of 2006 people nationwide in the results when compared to younger grade children and older grade in the low and the higher. Subjective oral health awareness, high of viscosity junk food snacks, fruit, gender, dental visits and humidity, oral health education classes, including the variable of attitude was 6(p<0.05). Their higher awareness of dental health, oral health education classes ever the more positive attitude to DMFT index was statistically significant(p<0.05). Solid in the habit of eating junk food snacks to the students of a liquid relative to student intake was higher DMFT index indices(p<0.05). Conclusions : We could reason that one's oral health recognition and behavior should have a relationship with his(her) DMFT index.

• Clinical and Experimental Reproductive Medicine
• /
• 제25권3호
• /
• pp.281-286
• /
• 1998

Premature ovarian failure is a condition causing amenorrhea, hypoestrogenism, and elevated gonadotropins in women younger than 40 years. Many causes of premature ovarian failure were reported, including genetic abnormalities, enzymatic defects, defects in gonadotropin secretion or action, autoimmune disorders, physical and idiopathic causes. Recently, Finnish group reported a point mutation in the follicle stimulating hormone (FSH) receptor gene in premature ovarian failure patients. But it was reported that the group from United States could not find any mutation in FSH receptor gene. So we analysed C566T point mutation of FSH receptor gene using restriction fragment length polymorphism (RFLP) and compared the result between premature ovarian failure patient with idiopathic and known causes. But we did not find 556C${\rightarrow}$T mutation in the FSH receptor gene in both groups. These findings suggest that the missense mutation in the human FSH receptor gene reported in Finnish women with premature ovarian failure is uncommon in Korean women with premature ovarian failure.