• Title/Summary/Keyword: Plodia interpunctella

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Changes of Total Protein during the Metamorphosis of the Indian meal moth, Plodia interpunctella Hubner (화랑곡나방의 변태(變態)에 따른 총단백질(總蛋白質)의 변화(變化))

  • Lee, Sang-Suk;Lee, Kyung-Ro
    • Journal of radiological science and technology
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    • v.3 no.1
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    • pp.107-113
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    • 1980
  • The concentration of total protein during the metamorphosis of the Indian meal moth, Plodia interpunctella $H\ddot{u}bner$ was measured using micro-Kjeldahl method by Oser(1965). Healthy specimens were chosen as samples at each developmental stages: the 1st instar larva, the 3rd inster larva, final instar larva, pupae 2, 6, 10 days and the adult 1 day. The total protein concentration decreased gradually until the two day pupal stage and increased at the six day pupal stage. In the one day dault stage, the concentration reached a maximum. In conclusion, changes of total protein concentation through the course of the physiological cycle form a U-shaped curve when graphed against the histolysis of larval organs and the histogenesis of adult organs. In view of controlling pest, the U-shaped pattern of physiological activity indicates that control will be the most effective at the time of the most physiological weakness manifested at the two day pupal stage.

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Influence of Reactive Oxygen Species Produced by Chlorine Dioxide on Induction of Insect Cell Apoptosis (이산화염소의 활성산소 유발에 따른 곤충 세포의 아폽토시스)

  • Kim, Minhyun;Kumar, Sunil;Kwon, Hyeok;Kim, Wook;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.55 no.3
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    • pp.267-275
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    • 2016
  • Chlorine dioxide has an insecticidal activity via its production of reactive oxygen species (ROS). Its cytotoxic activity has been regarded as a main cause of the insecticidal activity. This study tested a hypothesis that cytotoxicity of chlorine dioxide is resulted from its induction of apoptosis against target cells using ROS. Injection of chlorine dioxide significantly reduced total hemocyte counts of Plodia interpunctella larvae and subsequently killed the larvae. To analyze the cytotoxicity with respect to apoptosis, terminal deoxyribonucleotidyl transferase nick end translation (TUNEL) assay was performed. An insect cell line (Sf9) cells were exposed to different concentrations of chlorine dioxide. TUNEL assay showed that chlorine dioxide induced significant apoptosis of Sf9 cells in a dose-dependent manner. When different concentrations of chlorine dioxide were injected to larvae of P. interpunctella, it showed a dose-dependent induction of apoptosis against hemocytes. However, addition of vitamin E significantly suppressed the apoptosis induction and insecticidal activity of chlorine dioxide in a dose-dependent manner. These results suggest that cytotoxicity of chlorine dioxide is resulted from its induction of apoptosis against insect cells using ROS.

Effects of Type and Thickness of Flexible Packaging Films on Perforation by Plodia interpuntella (유연포장 필름의 종류 및 두께에 따른 화랑곡나방 침투율 연구)

  • Lee, Soo Hyun;Kwon, Sang-Jo;Lee, Sang Eun;Kim, Jeong-Heon;Lee, Jung-Soo;Na, Ja Hyun;Han, Jaejoon
    • Korean Journal of Food Science and Technology
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    • v.46 no.6
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    • pp.739-742
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    • 2014
  • This study investigated the effect of perforation by the Indian meal moth (Plodia interpunctella) larvae on various flexible food-packaging films, in relation to their thickness and type. Among the various flexible packaging films, polyethylene (PE), aluminum foil (AF), polypropylene (PP), polystyrene (PS), and polyethylene terephthalate (PET) were selected for this study due to their wide usage in food packaging. Based on their thickness, film penetration by P. interpunctella larvae was measured as in following order: PP, $20{\mu}m$; AF, $9{\mu}m$; PET, $12{\mu}m$; PP, $30{\mu}m$; PS, $30{\mu}m$; PE, $40{\mu}m$; PE, $35{\mu}m$; PS, $60{\mu}m$; and PET, $16{\mu}m$. P. interpunctella larvae rapidly penetrated through the packaging films regardless of their thickness and type. In particular, it was observed that PP of $20{\mu}m$ and PS of $30{\mu}m$ were completely penetrated by P. interpunctella larvae within 72 h, rendering thin PP and PS films less valuable as anti-insect packaging films. Our results show that the perforations by P. interpunctella larvae were observed in the thin films. These results imply that each packaging film has a marginal thickness against the perforations by P. interpunctella larvae.

Development of a Fennel (Foeniculum vulgare) Oil-based Anti-insect Sachet to Prevent the Indian Meal Moth (Plodia interpunctella) (화랑곡나방 유충 방제를 위한 회향오일 기반 방충향낭 개발)

  • Lee, Soo-Hyun;Jo, Heon-Joo;Lee, Yun-Jeong;Han, Jaejoon
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.19 no.2
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    • pp.81-85
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    • 2013
  • The stored-product insects have been a serious problem during the entire process of distribution on the food industry. Especially, the Indian meal moth (Plodia interpunctella) is one of stored-product insects which causes harm through penetrating into the food packaging. The objective of this study was to develop the anti-insect packaging material with fennel (Foeniculum vulgare) oil (FO). The FO has been selected for insecticidal substance against P. interpunctella, which was tested by fumigant toxicity assay. An anti-insect sachet was prepared by FO and filterpaper placed in a small paper bag. Repellent test was performed to evaluate the repellent activity of anti-insect sachet. In addition, the controlled release of FO from the anti-insect sachet was determined at $28^{\circ}C$ by gas chromatography (GC). It was demonstrated that FO was an effective substance against P. interpunctella. The mortality of FO was 56% at 800 mg/0.5 L in 120 h. In repellent test, the FO sachet showed effective repellency against P. interpunctella. The developed anti-insect sachet could be a promising source for insect repellent materials in food packaging.

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Effect of Temperature on the Development of Bracon hebetor (Hymenoptera: Braconidae) Parasitizing Indianmeal Moth (Lepidoptera: Pyralidae) (화랑곡나방(나비목: 명나방과)에 기생한 보리나방살이고치벌 (벌목: 고치벌과)의 발육과 온도와의 관계)

  • 김나경;나자현;류문일
    • Korean journal of applied entomology
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    • v.39 no.4
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    • pp.275-279
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    • 2000
  • Development of Bracon hebetor Say parasitizing Indianmeal moth (Plodia interpunctella (Hubner) was studied at five temperature conditions (17, 20, 25, 28 and 32$\pm$0.5$^{\circ}C$) under a photoperiod of 16 : 8 (L : D). Developmental period (mean$\pm$s.e.) of B. hebetor from egg to eclosion decreased from 28.6$\pm$0.50 to 9.3 $\pm$0.09 days and 28.1 $\pm$0.51 to 9.2$\pm$0.09 days for female and male, respectively, as the temperature increased from 17 to $32^{\circ}C$. The combination model provided a good description of the relationship between temperature and development. The low temperature thresholds were estimated to be 14.0, 12.8, 15.1$^{\circ}C$ for development of egg, larva and pupa. The thresholds for normal development (outside of the boundary layer of the development) were 14.0, 17.5, $15.1^{\circ}C$ for egg, larva and pupa, respectively, indicating that the larval stage is more sensitive to the low temperature than the other stages. The results suggested that the present B. hebetor population could be another ecological race adapting to the seasonal temperature conditions of this area.

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Studies on the Free Amino Acids of Indian Meal Moth (한국산 화랑곡 나방의 유리 아미노산에 관한 연구)

  • 이경로
    • The Korean Journal of Zoology
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    • v.7 no.1
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    • pp.19-22
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    • 1964
  • The free amino acid content of Indian meal moth (Plodia interpunctella HUBNER) was analysed at various developmental stages by means of paper chromatography. 1) The free amino acids : present are alanine , arginine, aspartic acid, glutamic acid, glycine, histidine, leucine, methionine, proline, serine, threonine, tyrosine and valine. 2) Proline was detectable only in the acid-hydrolyzed Indian meal moth. 3) Arginine was clearly detected only in the larva stage. 4) Tyrosine methionine and valine were increased in the pupa stage. 5) Serine, glycine and tyrosine were present in high concentration in all stages.

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Protection of Chocolate Products from Indian Meal Moth by Adding Cinnamon Extract to the Adhesive on the Wrapping

  • Na, Ja-Hyun;Hong, Euk-Il;Ryoo, Mun-Il
    • Korean journal of applied entomology
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    • v.47 no.4
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    • pp.491-495
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    • 2008
  • Adding cinnamon (Cinnamonum cassia Blume) extract into the adhesive used to affix stickers to a chocolate package is an effective method for protecting chocolate products from infestation by the Indian meal moth (Plodia interpunctella Hubner). Chocolate packages treated with adhesive including 0.02% and 0.025% of cinnamon extract were not infested with the Indian meal moth for up to 30 days at $28.1^{\circ}C$ and 70-75% RH, whereas 100% of the packages without the extract were infested in the no-choice test. Chocolate packages treated with adhesive including 0.02% and 0.025% of cinnamon extract in the quadruple choice test were not infested with the Indian meal moth for up to 60 days at $28.1^{\circ}C$ and 70-75% RH, whereas 100% of the packages without the extract were infested. A panel test showed that the cinnamon extract treatment would not affect consumers' choices.