• 제목/요약/키워드: Plevrotus osteatus

검색결과 1건 처리시간 0.023초

Pleurotus ostreatus의 laccase 작용특성 (Characterization of laccase from pleurotus ostreatus)

  • 김규중;신광수;맹진수;강사욱;하영칠;홍순우
    • 미생물학회지
    • /
    • 제25권2호
    • /
    • pp.148-156
    • /
    • 1987
  • Extracellular laccase (E.C. 1.10.3.2) from the culture filtrate of Pleurotus ostreatus was purified by ammonium sulfate precipctation, protamine sulfate precipitation, DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-100 gel permeation chromatography. The molecular weight of the enzyme was estimated by SDS-polyacrylamide gel electrophoresis to be 58,000 and the isoelectric point was 3.75. The optimum temperature for the enzyme was about $45^{\circ}C$ and the optimum pH was 6.5. The enzyme was found to be stable at temperature below $35^{\circ}C$ and rapidly inactivated at higher temperatures. Km values for ferulic acid, vanillic acid, dihydroxyphenylalanine (DOPA) were 48.6.$\mu$M, 0.52mM, and 2.73mM, respectively, which indicates that the enzyme has much higher affinity towards ferulic acid. The reaction products of the enzyme were separated by TLC and HPLC.

  • PDF