• Title/Summary/Keyword: Plecoglossus altivelis altivelis

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Applicability of Next-Generation Sequencing for Analysis of Stomach Contents in Fish (Next-Generation Sequencing을 활용한 어류 위 내용물 분석의 적용 가능성)

  • Chae-Jin Park;Seonbin Yun;Hyeon-Sik Lee;Seoyun Jang;Kang-Hui Kim;Donghyun Hong;Gea-Jae Joo
    • Korean Journal of Ecology and Environment
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    • v.56 no.1
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    • pp.104-125
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    • 2023
  • The predator-prey interaction in freshwater ecosystems is a crucial area in the ecological study field and one of example to find such interaction is to investigate stomach contents. However, traditional method through visual inspection often induce misidentification, as it depends critically on intactness of physically visible data. In this study, we utilized Next-Generations Sequencing (NGS) technology to test the applicability stomach content analysis and overcome such limitation. NGS was applied to analyze the stomach contents of the Hemibarbus labeo, Tachysurus fulvidraco, and Plecoglossus altivelis collected in the lower part of Nakdong River. As a result, T. fulvidraco had a higher number of Animalia operational taxonomic units (OTUs) intake rate than H. labeo. At the same time, P. altivelis had higher number of Plantae OTUs intake rate than T. fulvidraco and higher Protozoa OTUs intake rate than H. labeo respectively. Therefore, NGS technology application enable to overcome traditional method's limitation and discover hidden interspecific interaction which can further be used in appropriate habitat assessment.

Histopathology of a microspridian infection in ayu, Plecoglossus altivelis, from southern Korea (양식 은어의 미포자충 감염예에 대한 병리조직학적 관찰)

  • Jo, Byeong-Yeol;Gang, Hyeong-Gil;Gang, Hyo-Ju;Ryu, Gap-Min;Lee, Jae-Yeong;Park, Nam-Gyu;Heo, Min-Do
    • Journal of fish pathology
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    • v.16 no.1
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    • pp.23-30
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    • 2003
  • On September in 1998, a microsporidian infection was recognized in ayu, Plecoglossus altivelis, farmed on Kyongnam province of South Korea. Cumulative mortality was around 10% in 10 days. Infected fish which were piping for air near the surface of the water or in the asphyxic, lethargic condition revealed darkening of body and abdominal distention. Numerous whitish nodules up to 3mm in size were observed throughout most of body organs and tissues including gill, operculum, peritoneal wall and organs. Xenomas were also histologically confirmed in multiple internal organs with the evidences suggesting circulatory disturbance. Based on the morphology of spore and xenoma, and the distribution of xenomas in organs and tissues, this disease was diagnosed to be a microsporidiosis caused by Glugea plecoglossi. The mortality might be deeply related to the local circulatory disturbance by xemonas rather than the mechano-chemical effect of xenomas on adjacent tissues.

Growth of a Land-locked Ayu, Plecoglossus altivels (Pisces: Osmeridae) and Weir Obstruction in Lake Okjeong, Korea (옥정호 육봉형 은어 Plecoglossus altivelis (Pisces: Osmeridae)의 성장과 보에 따른 영향)

  • Ko, Myeong-Hun;Kim, Ik-Soo;Park, Jong-Yeong;Lee, Yong-Joo
    • Korean Journal of Ichthyology
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    • v.19 no.2
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    • pp.142-153
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    • 2007
  • Fertilized eggs of Plecoglossus altivelis over than 1,000,000 were introduced into Okjeong, Jeollabuk-do, Korea by K-water, 2001. Since then, this lake and the neighbouring tributaries gave sufficient habitat to maintain their life cycle which are being the land-locked. A land-locked ayu of annual life is ascending and descending from Lake Okjeong to streams for spawning and growth. Among the streams, the Churyeong stream reaching about 32 km in length is a major site for growth and spawning. But the land-locked ayu was no longer seen in the upper regions of the Churyeong stream over than about 12 km distance from Lake Okjeong. Because there were 11 weirs along with the Churyeong stream, it was difficult for them to move freely up to upper regions, and it causes irregular growth condition although they must be actively growing with feeding for spawning. During three survey years (2004 to 2006), in lowest rainfall-recorded 2005, the ayu population was not able to move up actively across the weirs and just stayed around the weirs, and finally it let them grow slowly and unstably. Also although there are 11 weirs, only 3 fish ways existed. Such environmental factors will seriously affect growth, spawning and size of ayu population in this area.

Changes in fish species composition after fishway improvement in Songrim weir, Yeongok stream (연곡천 송림보에서 어도의 개선에 따른 어류 종 조성 변화)

  • Yun, Young-Jin;Kim, Ji Yoon;Kim, Hye-Jin;Bae, Dae-Yeol;Park, Gu Seong;Nam, Chang Dong;Lim, Kyung Hun;Lee, Moon-Yong;Lee, Seong-Yong;Moon, Kyeong-Do;Lee, Eui-Haeng;An, Kwang-Guk
    • Korean Journal of Environmental Biology
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    • v.39 no.2
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    • pp.195-206
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    • 2021
  • In 2020, South Korea initiated research and development of a longitudinal connectivity evaluation between upstream and downstream based on stream ecosystem health. This study analyzed the migration of upstream and downstream migratory fish species, fish distribution characteristics, trophic guilds, tolerance guilds, and species composition changes from 2015 to 2020 at Songrim weir in Yeongok stream, where the cross-structure of an ice harbor-type fishway for fish movement was recently improved. A total of 5,136 fish, including 36 species, were collected and three major migratory fishes were identified, namely, Tribolodon hakonensis, Plecoglossus altivelis altivelis, and Oncorhynchus keta. According to the comparative analysis before (Pre-I) and after (Post-I) improvement of the fishway, the relative abundance of primary freshwater fish increased in the upstream section, while the number of migratory fishes decreased. The fish species that used the fishway in the Songrim weir were Tribolodon hakonensis (58.4%) and Plecoglossus altivelis altivelis(11.8%). According to the Wilcoxon Signed-Rank Test migratory fish showed a statistically significant difference (p<0.05) in the upstream and downstream, showing a biological improvement effect of the crossstructure. On the other hand, the annual change of migratory fish based on the MannKendall trend test did not significantly increase or decrease (p>0.05). Therefore, in the fish passage improvement project, it is necessary not only for physical, hydrological, and structural tests, but also for pre- and post-biological tests on the use and improvement effect of fishway.

Early Gonadogenesis and Sex Differentiation in Sweet Fish, Plecoglossus altivelis (은어, Plecoglossus altivelis의 초기 생식소 형성 및 성분화)

  • 방인철;박상용;이윤아;이철호;김성연;김경길
    • Journal of Aquaculture
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    • v.13 no.3
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    • pp.215-222
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    • 2000
  • The primordial germ cell of the sweet fish was recognized from the 2-day old fry (tl : 0.66 cm), when it began to protrude into peritoneal cavity between meaonephric duct and gut. The primordial gonad, with the formation of genital ridge, developed on the 30-day old fry. Ovarian differentiation was identified by the presence of ovarian cavity and meiotic oocytes from the 90-day old fry (tl : 3.42 cm). Testicular differentiation was identified by the presence of spermatogonial cells with efferent duct from the 100-day old fry (11 : 4.50 cm). Hence the sweet fish belongs to the differentiated type of gonochoristic teleost.

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THE RELATIONSHIP BETWEEN TOTAL LENGTH, BODY WEIGHT AND CONDITION FACTOR OF PLECOGLOSSUS ALTIVELIS IN CHUNJI STREAM BEFORE AND AFTER SPAWNING PERIOD (산란기를 전후한 천지천산 은어, Plecoglossus altivelis의 비만도와 체장 체중관계)

  • KIM Eul Bae;CHUNG Sang Chul
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.9 no.4
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    • pp.251-255
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    • 1976
  • Three hundred and sixty two specimens of Plecoglossus altivelis were randomly sampled from Chunji Stream in Seogwipo, Cheju-Do, during the period from 25 Aug., 1973 to 14 Feb., 1974 to investigate the relationship between total length and dody weight as well as the condition factor during the spawning period. Results obtained are summarized as follows : The relationships between total length and body weight are calculated as below, respectively : $W=0.008639\;L^{3.1806}$ : Prior to spawning period $W=0.01830\;L^{2.7689}$ : During spawning period The above equations differ each other significantly. Nevertheless, the significant difference is not found between sexes. The mean body length decreased during the spawning season. The fact seems to be caused by the death of large fish after spawning. The condition factor was $13.45\%_{\circ}$ on the average in August. The value decreased continuously from September, giving the minimum mean value of $10.25\%_{\circ}$ on Nobember 29. But the value increased again, showing $11.18\%_{\circ}$ in next February. Majority of the fish survived until next year was small ranging from 11 to 12cm in total length.

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Growth and Body Composition of Larval Ayu (Plecoglossus altivelis) Fed the Micro-diets Containing Kluyveromyces fragilis and Candida utilis (미립자사료에 효모, Kluyveromyces fragilis와 Candida utilis의 첨가가 은어 자어의 성장과 체성분에 미치는 영향)

  • LEE Sang-Min;KIM Dong-Ju;KIM Kyoung-Duck;KIM Joong-Kyun;LEE Jong Ha
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.1
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    • pp.20-24
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    • 2000
  • A 7-week growth trial was conducted to investigate the effects of yeasts (Kluyveromyces fragilis and Candida utilis) with or without cell wall chemical treatment (protoplasted) in formulated diets on growth and body composition of larval ayu (Plecoglossus altivelis). Three replicate groups of ap average weighing 100 mg were fed diets containing each level ($5{\%}$) of K. fragilis, protoplasted K. fragilis, C. utilis, protoplasted C. utilis or brewer's yeast as an additive. Survival rate of fish fed the diet containing protoplasted K. fragilis, C. utilis or protoplasted C. utilis was higher than that of fish tea the control diet (P<0.05). Body weight .gain of fish fed the diet containing protoplasted K. fragilis was higher than that of fish fed the control diet (P<0.05). Crude protein and ash contents of Ssh were not significantly affected by the different dietary yeasts (P>0.05), On the other hand, crude lipid content of fish fed the diet containing K. fragilis, protoplasted K. fragilis or brewer's yeast was higher than that of fish fed the control diet (P<0.05). Amino acids composition of fish was not significantly affected by the different dietary yeasts (P>0.05), except aspartic acid. The results suggest that protoplasted K. fragilis as an additive in micro-formulated diet can improve weight gain and body quality of larval ayu.

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Effects of Dietary Perilla Oil and Enteromorpha compressa Meal on Growth, Fatty Acid Composition and Hematology of the Cultured Sweet Smelt (Plecoglossus altivelis)

  • Jeong Woo-Geon;Moon Soo-Kyung;Jeong Bo-Young;Jang Whei-Sook;Kim In-Soo;Maita Masashi;Lim Dong-Hoon;Lee Sang-Min
    • Fisheries and Aquatic Sciences
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    • v.7 no.1
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    • pp.1-9
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    • 2004
  • Sweet smelt (Plecoglossus altivelis) were fed four different diets supplemented with either perilla oil $(2.0\%)$ rich in 18:3n-3 (CP), and perilla oil and Enteromorpha compressa meal $(2.0\%)$ (CPA), soybean oil rich in 18:2n-6 (CO), or soybean oil and algal meal (CA) for 4 weeks. The growth performance, fatty acid composition of muscle, plasma lipid peroxidation and blood components of the sweet smelt were then determined. The specific growth rate and feed efficiency in the fish fed the CPA diet were the highest, while the other groups showed similar results. The fatty acid composition of muscle in sweet smelt reflected the dietary lipids; 18:3n-3 was higher in the fish fed the CP and CPA diets, and 18:2n-6 was higher in the fish fed the CO and CA diets. The other fatty acid profiles presented almost no differences with respect to the diet composition. The fish fed the CA, CP and CPA diets contained significantly lower levels of triglyceride, thiobarbituric acid-reactive substances and hydroxyl radical in their plasma than that fed the CO diet. Phagocytic activity was the highest in the fish fed the CPA diet and higher in those of the fish fed the CP and CA diets compared to the CO diet group. The results from this study suggest that a dietary supplement of $2.0\%$ perilla oil together with $2.0\%$ E. compressa meal may improve the growth and health of cultured sweet smelt.