• Korean Journal of Microbiology
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• v.38 no.3
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• pp.221-229
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• 2002

Japanese encephalitis virus (JEV) persistence was established and maintained in Vero cell culture for over 1 year. Eleven clones of plaque morphology mutant JEV, with large and small plaque sizes, were obtained from the cell culture supernatant. Genomic RNA replication efficiency of the mutants in naive Vero cell appeared to correspond to their different plaque sizes. No significant changes in envelop protein ORF or in non-coding regions at both ends of the RNA genome suggested that there could be an unidentified factor(s) playing role in JEV attenuation. Unlike to the replication of wild-type JEV, the mutants did not induce severe degree of cytopathic effect in Vero cells upon infection. While obvious decrease of Bcl-2 and its mRNA expression and sharp increase of p53 in naive Vero cells infected with either wild-type JEV or the large plaque-forming mutant, those changes were not observed with the small plaque-forming one. Together with these observation, internucleosomal DNA fragmentation and chromosomal DNA profile in the Vero cells infected with the mutants suggest that an overall changes in cytopathic effect in the plaque morphology mutants-infected cells should be primarily due to the reduced genomic RNA replication and the compromised degree of p53-independent apoptosis by the virus infection at least in part.

• Korean Journal of Soil Science and Fertilizer
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• v.37 no.6
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• pp.371-377
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• 2004

Sulfate adsorption in forest soils is a process of sulfur dynamics playing an important role in plant uptake, cation movement, acid neutralization capacity and so on. The relationship between sulfate adsorption and some physicochemical properties of four forest soils was investigated. Extractable sulfate contents and sulfate adsorption capacity (SAC) in the forest soils varied much among study sites. Extractable sulfate contents were more in sub-surface soils with lower organic matter and greater Al and Fe oxides than in surface soils. The average contents of $Al_d$ and $Fe_d$ in the sub-surface soils were 8.49 and $12.45g\;kg^{-1}$, respectively. Soil pH, cation exchange capacity and clay content were positively correlated with the extractable sulfate contents and SAC. Organic carbon content, however, was negatively correlated with the extractable sulfate contents, implying the competitive adsorption of sulfate with soil organic matter. Considerably significant correlation was found between inorganic + amorphous Al and Fe oxides and the sulfate adsorption, but crystalline Al and other fractions of Fe oxide showed no correlation. Relatively close relationship between the adsorbed sulfates and soil pH, cation exchange capacity, or amorphous Al oxides indicates that the accelerated soil acidification may substantially reduce the potential for sulfate adsorption contributing to sulfur flux in forest ecosystems.

• Journal of Life Science
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• v.17 no.3 s.83
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• pp.420-426
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• 2007

In the present investigation, we studied the modulating effects of (-)-epigallocatechin-3-gallate(EGCG) on the differentiation of mouse C2C12 myoblasts. We found that the strong inhibitory effect of EGCG on DNA methyltransferase-mediated DNA methylation induced transdifferentiation of C2C12 myoblasts into smooth muscle cells demonstrated by both morphological changes and immunofluorescent staining. C2C12 myoblasts treated with EGCG for 4 days expressed smooth muscle ${\alpha}-actin$ protein. Real-time PCR data revealed that smooth muscle ${\alpha}-actin$ mRNA was induced by EGCG treated C2C12 myoblasts in a concentration-dependent manner. Smooth muscle ${\alpha}-actin$ mRNA concentration increased 330% and 490% after 2 and 3 days of 50 ${\mu}M$ of EGCG treatment. The expression of another smooth muscle marker, transgelin, mRNA was also increased up to 9-fold by 4 days of EGCG treatment compared with control in a concentration-dependent manner. These results suggested that C2C12 enables to transdifferentiate into smooth muscle when gene expression patterns are changed by the inhibition of DNA methylation induced by EGCG. In conclusion, transdifferentiation of C2C12 myoblasts into smooth muscle is resulted from the modulating effects of EGCG on DNA methylation which subsequently results in changing the expression pattern of several genes playing a critical role in the differentiation of C2C12 myoblasts.

• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.19-25
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• 2008

To evaluate the functions of vegetation mat of artificial floating island (AFI) installed in Lake Paldang, nutrients, such as total phosphorus (TP), dissolved inorganic phosphorus (DIP), total nitrogen (TN) and nitrate $(NO_3)$ and microbial factors such as total bacterial numbers, active bacterial numbers and exoenzymatic activities of $\beta$-glucosidase and phosphatase in pore water of medium and bulk lake water were analyzed. The concentration of TN and $NO_3$ in pore water ranged from 4.4 to 7.5mg $L^{-1}$ from 1.2 to 3.8mg $L^{-1}$ respectively, which were ca. 2 times higher than those of lake water. The ranges of TP and DIP of were $1.4\sim4.1mg\;L^{-1}$ and $0.003\sim0.137mg\;L^{-1}$ in pore water of media which were $4\sim25$ and 5 times higher than those of lake water, respectively. The numbers of total bacteria and active bacteria in pore waterwere about 10 times higher than those of laker water. Also, both phosphatase and $\beta$-glucosidase activities of pore water were on an average 10 times higher than those of lake water. These results suggest that the bacteria were playing important role for nutrients concentrating and cycling in media of artificial floating island. And the medium of artificial floating island contained newly created microbial ecosystem, which is responsible for sustaining the growth of macrophytes and the creation of new aquatic ecosystem.

• The Journal of the Korea Contents Association
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• v.17 no.5
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• pp.234-242
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• 2017

This study selected 60 board games that have a high sales rate and awareness in the board game market, and analyzed 37 board games that were selected through the advice of experts. According to the analysis of general characteristics, there was the highest number of board games that more than 2 people could participate and people from ages 7 to 11 could use, and the most common play time was from 20 minutes to 30 minutes. Also, there was the largest number of board games produced in 2000s, and Germany was the most common producer of board games. Next, the content analysis showed that abstract strategy was the most common game genre, and cognitive domain was the most common in the development area. The analysis based on how to play the game showed that games that had to go through 4 stages were the most common. Card games were the most common form of game, group was the most common in organization form, and reaching goal was most common among result analysis method. According to the analysis of correlation by items, the number of people and work analysis had a statistical correlation, and the playing age, time, and genre had a correlation. The origin of the game and game genre were also correlated to each other, and game form, game genre, organization form, and result analysis had a statistical correlation as well. The purpose of this study is to analyze the board game in terms of structural characteristics and to provide a foundation for future research.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.9
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• pp.74-82
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• 2016

The term research institute spin-offs refers to new firms created by public research institutes. These spin-offs are different from other start-ups in two respects: on the one hand, they should be located in the Special Research and Development Zones and, on the other hand, these firms are supposed to commercialize the results of public R&D activities. These spin-off firms show higher rates of survival and job creation than general new firms, which means that their contribution to economic growth is not negligible. The present study analyzes the factors affecting research institute spin-offs using a random effect panel logit model and negative binomial model. From previous studies, four elements are identified as playing an important role in the commercialization of public R&D through spin-offs, namely their organizational character, research capability, technological character, and geographical location. The empirical results demonstrate that government research institutes with more researchers and patents are more likely to create new firms. In addition, the location of the institutes significantly affects the probability of their creating spin-offs and their number. When the technological stage and TLO size are considered, however, it turns out that the number of researchers and technological stage play important roles in the spin-offs.

• Journal of Korea Multimedia Society
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• v.6 no.2
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• pp.208-215
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• 2003

For playing a multimedia presentation in a Internet, the case that the presentation QoS(Quality of Services) at a destination nay be different from the QoS of multimedia data at the source occurs frequently. In this case, the process of trancoding the multimedia data at the source Into the multimedia data satisfying the QoS at the destination should be requited. In addition, even the presentation description having the homogeneous QoS at both sides may have different transcoding paths due to the limitation of display terminals or network bandwidth. That is, for a multimedia description, it is required to regenerate a proper transcoding path whenever the displaying terminals or the network environment gets decided. And the delay time required to go through the transcoding path may affect the playability of the give presentation. Therefore it should be checked whether the presentation requiring a transcoding process is able to be played in a real time. In this paper, the algorithm for generating all the possible transcoding paths for a given multimedia description under a fixed set of transcoders and the network environment is proposed. The algorithm adopts the concept of QoS transition diagram to Prevent from a trancoding Path being cycled by the repetition of a cyclic Path which generates the same QoS of multimedia data as its input QoS. By eliminating all the cyclic Paths, the algorithm can guarantee the termination of the process. And for the playability check, a method of computing the transcoding time and the delay lime between logical data units are proposed.Finally all the proposed methods were implemented in the stream engine, called TransCore and the presentation-authoring tool, called VIP, we had developed. And the test results with sample scenarios were presented at the last.

• The Korean Nurse
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• v.37 no.2
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• pp.44-52
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• 1998

The purpose of this study is to inquire the people's views on nursing for nurses, correct the image of nurse and take it as basis to be applied on nursing education examining the image of nursing on Television drama playing important role of mass media. 22 nurses of the characters in drama is applied to the analysis object of this study by selecting 6 dramas of Television ones the nurse play on the prime time from June 1 to August 31 in 1997. Contents analysis method was used in Data Analysis, 4 items was used after Coders previously modify and compensate it based on research documents of 1m Milim(1996) 2 Coders made the Coding the article on each person by them seeing the recorded film making the Coding Paper each items is written by the character. The average of reliability degree was 90% which measured the reliability degree by the mathod of Holsti. The statisic method of frequency, percentage was used SPSS Program in data processing The results were as follows. 1. Relative importance of 86.2% nurses in drama was depicted as extra characters 2. The affair attitude of nurses shown on drama was revealed as mechanical(84.7%), passive(45.5%), dependent(54.4%) unkind(68.2%). 3. The activity of nurses was classified with professional! simple affair. The professional affairs such as I.V., Blood Pressure Check, Rounding, Nursing Recording, Patient Education, Assist of Operation, Assistant meal of Patient, etc is mainly depicted and the screen of simple affair such as Receiving telephone, Carrying Tray or Dragging, Stretcher Car, Dressing Car and or Wheel Chair than professional affair. 4. The appearance feature of nurses was shown on thin physique(68.2%), common stature(68.2), dirty costume(45.4%), common appearance(81.9%), unnoble action(63.6%). The image of nurses is illuminated as the exterial scene of technical affair such as assisting the doctors and affair focused on accident and educational activity of nureses or extended role is nor depicted on Television drama. Therefore, the people regard the nurse as sexual object with good appearance than professional worker working professional nursing We want the following, epigraph based on above conclusion. 1. The continuous research is required on the image of nurse shown on various mass media. 2. The later research is required on appliction strategy of mass media for advancing the image of nurse. 3. The research to strengthen the objectivity by comparing analyzed data on drama & analyzing it is required 4. Through the deep study, the standard to show a concrete and professional work of nurses to scenario writers of TV drama is suggested by the association. 5. The monitoring about the mass media must be activated, not by some nurses, on a national scale and much study on the basis of this is needed.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.30 no.3
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• pp.57-67
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• 2012

This study draws the following conclusions about the nature garden culture with crane that is a dynamic landscaping factor introduced into nature garden by analyzing the literatures on cranes written and landscapes painted in Chosun Dynasty. First, crane symbolizes such Buddhist, Taoist and Confucius media as appearance of Buddha, desire for Taoist hermit world and long life, and a solitary's life of proud loneliness. It has been recorded that crane was raised from the Three Nations Era till the Japanese occupation and Confucius scholars in Chosun Dynasty opened shows concerning crane or formed literature groups enjoying poems of it, and often using crane as their denial of going into government service. Second, in order to introduce crane to nature garden, people caught wild crane and made a fence and some kind of pond for their growth. In addition, crane was strictly managed by appointed slaves and they trained crane for dancing and then tended to allow a crane to play on the yard in terms of abstract meaning or got two cranes free in consideration of their ecology. Third, for more appreciation of crane and the expression of some symbolism matching for it, both plum and pine, which mean a solitary's life and long life respectively, were planted in nature garden. And, Confucius scholars in Chosun Dynasty also enjoyed their refined tastes with appreciation, napping, reading and playing the harp, accompanied by crane. As aforementioned, Confucius scholars in Chosun Dynasty did not only draw the meaning symbolized by cranes and write poems about such symbolic meaning, but also positively introduce crane into nature garden as a dynamic landscaping factor, so that they enjoyed synesthetic senses including looks, motions and sounds of cranes for their refined tastes.

• Journal of Distribution Research
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• v.7 no.1
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• pp.61-86
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• 2002

The number of people who perform their banking business on Internet has been increasing drastically during the recent period and the trend still continues. Banks and other types of financial institutions are now competing intensely with each other in attracting customers to this new innovative banking service. The marketers in charge of formulating and implementing the strategies for this new banking business need to understand the behavioral factors as well as the technical factors in order to succeed in attracting more customers to this new service. products. A deep understanding as to why and how the customers are adopting the new service developed on information technology is a prerequisite for a successful marketing effort. The purpose of this dissertation is to find out what factors are playing important roles in the customers'decision related to the adoption of the Internet banking services. The focus of the research is threefold. First. by testing the hypotheses proposed by the Technology Adoption Model, a well-known theory in the IT-related behavioral science, an analysis was made as to which factors are more important in affecting the attitudes and adoption of the Internet banking on the part of customers. Secondly, an analysis was made as to whether the three criteria of the Technographics- -the attitude toward new technology, level of income, and the motivational factor- -are useful as the criteria for segmenting the Korean users of the online banking. Thirdly, an analysis was made as to whether each customer group segmented by the criteria of the Technographics reveal a differential decision process and thus requires a differential approach strategywise.