• The Korean Journal of Malacology
• /
• v.30 no.4
• /
• pp.429-431
• /
• 2014

Since we reported a BLAST server for the mollusk in 2004, no work has reported the usability or modification of the server. To improve its usability, the BLAST server for the mollusk has been updated as version II (http://www.malacol.or.kr/blast) in the present study. The database was constructed by using the Intel server Platform ZSS130 dual Xeon 3.20 GHz CPU and Linux CentOS system and with NCBI WebBLAST package. We downloaded the mollusk nucleotide, amino acid, EST, GSS and mitochondrial genome sequences which can be opened through NCBI web BLAST and used them to build up the database. The updated database consists of 520,977 nucleotide sequences, 229,857 amino acid sequences, 586,498 EST sequences, 23,112 GSS and 565 mitochondrial genome sequences. Total database size is 1.2 GB. Furthermore, we have added repeat sequences, Escherichia coli sequences and vector sequences to facilitate data validation. The newly updated BLAST server for the mollusk will be useful for many malacological researchers as it will save time to identify and study various molluscan genes.

• Journal of the Korean Applied Science and Technology
• /
• v.32 no.2
• /
• pp.348-361
• /
• 2015

To reduce the effects of greenhouse gas (GHG) emissions, the government has announced the special platform of technologies as parts of an effort to minimize global climate change, and the government distributed biodiesel since 2006 as the further efforts. Although there are some debates about some quality specifications and unbalanced of source (44% from palm oil), more than 400kton/year of biodiesel was produced in 2013. Moreover the amounts will be increased when the RFS is activated. To solve the unbalanced situation and to achieve the diversity of feeds, it is essential that many researches should be considered. Especially, free and bond glycerines are one of the important properties seriously affected to the combustion system in vehicle & cold properties. Previous method (KS M 2412) couldn't cover the biodiesel derived from lauric oil($C_{12:0}$) such as PKO (Palm Kernel Oil), Coconut oil because those compositions are lighter than other conventional biodiesel sources. In this study, we review the existed method and figure out the factors should improve to analysis the glycerine from PKO and Coconut oil biodiesel. Modifying the analysis conditions to enhance the resolution and change the internal standards to avoid the overlapped- peaks between Capric acid ME ($C_{10:0}$) and standard#1(1,2,4-butantriol). From this revised method, we could solve the restrictions of previous methods. And check the possibility of new method to analyze the glycerine in biodiesel regardless of sources.

• Journal of Acupuncture Research
• /
• v.18 no.3
• /
• pp.56-68
• /
• 2001

Objective : Functional brain mapping study on acupuncture stimulation using the [14C]2-deoxyglucose([14C]2-DG) autoradiography provides quantitative data and visualized pathway in central nervous system(CNS). We aimed to investigate the neural pathway and spatial distribution of metabolic activity elicited in CNS on electroacupuncture stimulation using [14C]2-DG autoradiography. Methods : The study were divided into three groups by stimulation times. 45-mins stimulation group according to Sokoloffs method, 5-mins stimulation group according to Duncun's method, and 15-mins stimulation group. ;A venous catheter was equipped into right jugular vein. The rats (Sprague-Dawley rats, 230-260g) were kept fastened loosely on a holding platform without anesthesia. Electroacupuncture stimulation (5 ms, 2 Hz, 1~3 mA) were applied on the left Zusanli (ST36) acupoint and [14C]2-DG ($25{\mu}Ci/rat$) injection was performed through the catheter. After sacrifice, the brain and the spinal cord were made to sections for film image. The film images were digitalized as the isotope concentration based upon comparison of optical densities with that of the standards and normalized by the optical density of corpus callosum. Results : 1. 15-mins stimulation group was most effective among 3 experiments. 2. On 15-mins stimulation group, medial geniculate nucleus, intetpeduncular nucleus intermedius, ventral periolivary nucleus, caudal periolivary nucleus, medial superior olive, lateral paragigantocellular nucleus, including hypothalamic arcuate nucleus were increased by more than 25% (at least, p<0.05) by electroacupuncture stimulation. 3. Especially, the metabolism in hypothalamic arcuate nucleus was increased by 90% (p<0.05). 4. The fact that arcuate nucleus of hypothalamus might play a role of interconnection area between ascending and descending pathway of acupuncture stimulation was demonstrated visually. Conclusions : Advanced study on electroacupuncture stimulation elicited significant increase of metabolic activity in various nuclei of hypothalamus will provide the important experimental basis in research of the relationship between electroacupuncture stimulation and internal visceral functions.

• Journal of Ecology and Environment
• /
• v.43 no.2
• /
• pp.226-245
• /
• 2019

To understand the dynamics of radial growth of trees and micro-climate at a site of Korean fir (Abies koreana Wilson) forest on high-altitude area of Mt. Hallasan National Park, Jeju Island, Korea, high precision dendrometers were installed on the stems of Korean fir trees, and the sensors for measuring micro-climate of the forest at 10 minutes interval were also installed at the forest. Data from the sensors were sent to nodes, collected to a gateway wireless, and transmitted to a data server using mobile phone communication system. By analyzing the radial growth data for the trees during the growing season in 2016, we can estimate that the radial growth of Korean fir trees initiated in late April to early May and ceased in late August to early September, which indicates that period for the radial growth was about 4 months in 2016. It is interesting to observe that the daily ambient temperature and the daily soil temperature at the depth of 20 cm coincided with the values of about 10 ℃ when the radial growth of the trees initiated in 2016. When the radial growth ceased, the values of the ambient temperature went down below about 15 ℃ and 16 ℃, respectively. While the ambient temperature and the soil temperature are evaluated to be the good indicators for the initiation and the cessation of radial growth, it becomes clear that radii of tree stems showed diurnal growth patterns affected by diurnal change of ambient temperature. In addition, the wetting and drying of the surface of the tree stems affected by precipitation became the additional factors that affect the expansion and shrinkage of the tree stems at the forest site. While it is interesting to note that the interrelationships among the micro-climatic factors at the forest site were well explained through this study, it should be recognized that the precision monitoring made possible with the application of high resolution sensors in the measurement of the radial increment combined with the observation of 10 minutes interval with aids of information and communication technology in the ecosystem observation.

• Journal of the Economic Geographical Society of Korea
• /
• v.19 no.4
• /
• pp.597-615
• /
• 2016

Since 1997 the United Kingdom has pursued creative industry and creative city development in accordance with the New Labor Party policy, strengthening its cluster policy by assigning creative city policies to traditional manufacturing-oriented regions. Tech City in London, one of the most successful examples of digital clusters, is an area in which diverse ecosystems for venture business integration have been established, as the once barren space began to spontaneously develop. For this region, systematic linkages including universities, private companies, start-ups, and accelerators have been added, along with the UK government's active support system. As a result of this opportunity, the scale of the UK start-up ecosystem has significantly grown, the number of local companies has surged, and brand effect has greatly improved. Tech City is an example of a well-balanced combination of public effort and private governance, based on the region's historical background and its potential for growth. It is an effective coordination of public policy and private active investment, services, research, and education. The market platform for institutional technology and commercialization, and aggressive investment shares in the risk, have lead to its growth as a start-up and an innovative city. Britain's efforts to expand the nationwide cluster for the future-oriented digital economy is most noteworthy.

• Journal of Life Science
• /
• v.26 no.5
• /
• pp.620-631
• /
• 2016

Viroids are about 250-400 base pair of short single strand RNA fragments have been associated with economically important plant diseases. Due to the lack of protein expression capacity associated with replication, it is very difficult to diagnosis viroid diseases in serological methods. For detecting viroid at plants, molecular-based techniques such as agarose gel electrophoresis, polyacrylamide gel electrophoresis (PAGE), DNA-hybridization, blotting analysis and conventional RT-PCR are reliable. Real-time RT-PCR methods that grafted on RT-PCR methods with improved confirmation methods have been also utilized. However, they are still labor-intensive, time-consuming, and require personnel with expertise. Loop-mediated Isothermal Amplification (LAMP) method is a nucleic acid amplification method under the isothermal condition. The LAMP methodology has been reported to be simple, rapid, sensitive and field applicable in detecting a variety of pathogens. The results of LAMP method can be colorized by adding a visible material such as SYBR green I, Evagreen, Calcein, Berberine and Hydroxy naphthol blue (HNB) with simple equipment or naked eyes. The combination of LAMP method and nucleic pathogens, viroids, can be used to realize simple diagnosis platform for the genetic point-of care testing system. The aim at this review is to summary viroid-caused diseases and the simple visible approach for diagnosing viroids using Loop-mediated Isothermal Amplification (LAMP) method.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.45 no.4
• /
• pp.284-291
• /
• 2017

There is no doubt that advances in UAV technology have improved military performance. However, these advances require humans to adapt to new and complex operational systems. UAV has been rapidly expanding to a variety of fields such as reconnaissance, transportation, communication and aerial photographing recently. Also, with the development of UAV automation technology, one operator is able to supervisory-control multiple-UAVs. However, as the number of assigned UAV increases, the amount of information increases and this results in the workload of the operator increasing and deterioration in controlling performance. Accordingly, there is a need for a model to determine the level of overload an operator may encounter with regard to multiple-UAV but nationally this kind of research is currently lacking. Therefore, this paper provides an experimental platform for evaluating workload index effectiveness integrating multiple-UAV operational environments, GCS, and eye-tracking system followed by a limited survey of domestic and international studies of multi-UAV overload studies.

• Journal of Veterinary Clinics
• /
• v.27 no.2
• /
• pp.147-153
• /
• 2010

Bio-integration of cartilage grafts with subchondral bone is a significant clinical challenge. To date, the use of demineralized bone matrix (DBM) has been one of the most effective strategies for bone cell proliferation in vivo. Here, we investigated whether coculture of chondrocytes and DBM could serve as a single-platform system containing all the essential elements for purposive bone and cartilage induction. The aim of this study was to evaluate and compare the phenotype and proliferation of bovine chondrocytes cocultured with DBM in two different culture systems, pellet and alginate bead culture. In alginate bead culture, we observed an increase in chondrocyte number and formation of cell clusters. Typical chondrocytic phenotype was maintained for entire eight weeks. Histological analysis showed that chondrocytes maintained a typical round, plump morphology and there was a gradual increase in lacunae. Both coculture systems yielded an expanded cell population as compared to the controls (chondrocytes alone). The production of glycosaminoglycans was also increased in the coculture systems as compared to controls.

• Information Systems Review
• /
• v.12 no.3
• /
• pp.75-88
• /
• 2010

Business models in open market systems targeting smart phone users are determined by several important factors. First, by providing developers efficient technical platforms, it contains a setting for developers to learn, apply and improve the skills relating to the product category easily while they stay beyond a corporate boundary. Second, by the first condition, a huge population of talented developers becomes to join a specific open market where will invite more customers to use their applications. Hence it will attract more and more developer participants who will finally give a rise to a persistent market growth. Third, the evaluation system between platform providers and application producers, and one between application producers and application users may underlie the trust relationships between them. The research conducted a multiple embedded case study to test the success factors of open market based business models. It focused on smart phone game communities that have installed user evaluation, and feedback systems. The user innovation empowerment model within the social game networks has highlighted the theories on the roles and characteristics of lead users, and lead user network behaviors for future NPD participations.

• Journal of Intelligence and Information Systems
• /
• v.13 no.3
• /
• pp.17-29
• /
• 2007

Inference engine that performs the brain of software agent in next generation's web with various standards based on standard language of the web, XML has to understand SWRL (Semantic Web Rule Language) that is a language to express the rule in the Semantic Web. In this research, we want to develop a forward inference engine, SMART-F (SeMantic web Agent Reasoning Tools-Forward chaining inference engine) that uses SWRL as a rule express method, and OWL as a fact express method. In the traditional inference field, the Rete algorithm that improves effectiveness of forward rule inference by converting if-then rules to network structure is often used for forward inference. To apply this to the Semantic Web, we analyze the required functions for the SWRL-based forward inference, and design the forward inference algorithm that reflects required functions of next generation's Semantic Web deducted by Rete algorithm. And then, to secure each platform's independence and portability in the ubiquitous environment and overcome the gap of performance, we developed management tool of fact and rule base and forward inference engine. This is compatible with fact and rule base of SMART-B that was developed. So, this maximizes a practical use of knowledge in the next generation's Web environment.