• 한국동물위생학회지
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• 제34권2호
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• pp.159-166
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• 2011

This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

• Journal of Microbiology and Biotechnology
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• 제22권3호
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• pp.352-359
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• 2012

One hundred thirty-four putative Bacillus isolates were recovered from soybean rhizosphere soils of Nimar region to select effective zinc solubilizers for increased assimilation of zinc (Zn) in soybean seeds. These isolates were screened in vitro for zinc-solubilization ability on Tris-minimal agar medium supplemented separately with 0.1% zinc in the form of zinc oxide, zinc phosphate, and zinc carbonate. Of all, 9 isolates and a reference Bacillus cereus ATCC 13061 were characterized and identified as Bacillus species based on Gram-positive reaction, endospore-forming cells, and the presence of iso-$C_{15:0}$ and anteiso-$C_{15:0}$ as predominant fatty acids. On plate assay, two isolates KHBD-6 and KHBAR-1 showed a greater diameter of solubilization halo and colony diameter on all the three zinc compounds. The isolates KHBD-6, KHBAR-1, BDSD-2-2C, and KHTH-4-1 and the reference strain ATCC 13061 had higher soluble zinc concentration in liquid medium supplemented with zinc phosphate and zinc carbonate compounds as compared with the other isolates and uninoculated control. Evaluation under microcosm conditions showed that inoculation of isolates KHBD-6 (57.34 ${\mu}g/g$), KHBAR-1 (55.67 ${\mu}g/g$), and strain ATCC 13061 (53.10 ${\mu}g/g$) significantly increased the Zn concentration in soybean seeds as compared with the other isolates and uninoculated control (47.14 ${\mu}g/g$). This study suggests the occurrence of zinc-solubilizing Bacillus in soils of Nimar region and isolates KHBD-6 and KHBAR-1 were found to be promising zinc solubilizers for increased assimilation of Zn in soybean seeds.

• 대한소아치과학회지
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• 제25권3호
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• pp.539-544
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• 1998

저자는 열성형재료와 진공성형기를 이용하여 소아치과 임상에서 사용되는 수 종의 장치를 제작하였다. 전공성형기의 구입에 따른 경제적인 부담은 있으나, 기존의 제작법에 비해 제작이 간편하여 시간이 단축될 뿐 아니라 plate 의 두께가 균일하므로 finishing이 거의 필요하지 않으며 더욱 심미적인 장치를 제작할 수 있다. 또한 조직과 접촉하는 부위는 soft한 열성형재료를 사용하여 치아 이동에 유리할 뿐 아니라, 기존의 resin에 비해 불쾌한 맛이 적다는 장점이 있다. Monomer가 없어 조직에 자극이 적고 mutagenous potential이 없으므로 생체 적합성이 뛰어나 조직의 적응이 용이하다. 복잡하거나 특수한 기능 및 견고성이 필요한 악기능교정 장치 등에서는 그 사용이 제한된다는 한계점이 있으나, 소아치과 임상에서 사용되는 대부분의 장치를 쉽고 간편하게 제작할 수 있어 앞으로 임상에서 널리 이용될 수 있을 것으로 생각된다.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2004년도 춘계학술대회
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• pp.1452-1457
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• 2004

The water jet impingement cooling is one of the techniques to remove heat from high heat flux equipments. We investigate the local heat transfer of the confined water impinging jet and the effect of nozzle collar to enhance the heat transfer in the free surface jet and submerged jet. Boiling is initiated from the furthest downstream and the wall temperature increase is reduced with developing boiling, forming the flat temperature distributions. The reduction in the nozzle-to-surface distance for $H/W{\leq}1$ causes the significant increases and distribution changes in heat transfer. Developed boiling reduces the differences in heat transfer for various conditions. The nozzle collar is employed at the nozzle exit. The distances from heated surface to guide plate, $H_c$ are 0.25W, 0.5W and 1.0W. The liquid film thickness is reduced and the velocity of wall jet increase as decreased spacing of collar to heated surface. Heat transfer is enhanced for region from the stagnation to $x/W{\sim}8$ in the free surface jet and to $x/W{\sim}5$ in the submerged jet. For nucleate boiling region of further downstream, the heat transfer by the nozzle collar is decreased in submerged jet compare with higher velocity condition. It is because the increased velocity by collar is de-accelerated at downstream.

• 대한기관식도과학회:학술대회논문집
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• 대한기관식도과학회 1983년도 제17차 학술대회연제순서 및 초록
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• pp.8.1-8
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• 1983

후두격막증은 이를 선천성 및 후천성 후두격막증으로 분류하며 선천성 후두격막증은 후두의 발생과정에서 특히 태아기 7 ∼ 8주경에 후두개와 양측 피열연골 융기가 서로 합쳐진 T형 초기성문 (primitive glottis)이 태아기 10 주경에 이루어져야 할 전후분리가 장해됨으로서 선천성 후두질환의 약 3 %에서 발생되며 그 중 약 75 %가 성문부에서 발생되고 나머지 약 25 %는 성문상부 및 성문하부에서 발생된다. 한편 후천성 후두격막증은 그의 대부분이 외상이나 수술후의 상흔 등에서 합병되며 선천성에 비해서 격막이 비교적 두껍고 섬유성인 경향을 보인다. 후두격막증의 증상은 그 격막의 상태에 따라서 일정하지 않으나 대체로 그 격막형성이 아주 작은 경우에는 특별한 증상이 없으나 격막이 큰 경우에는 사성이나 호흡곤란을 유발하게 된다. 본 증의 치료 원칙으로서는 격막을 절제한 후 특히 성문의 성대전연합 (Anterior Commissure)부위의 재유착을 예방하는 것인데 현재까지의 시술방법으로서는 1924년 Haslinger가 수술후의 성대전 연합 사이로 엷은 은편(silver plate)을 삽입시켰던 례와 1950년 McNaught가 갑상연골절개술로 탄타룸 편(tantalum keel)을 삽입시켰던 례가 있으며 이밖에도 테후론편 (teflon keel)등이 삽입되거나 점막이식술 등이 시도되어 왔다. 저자들은 최근에 23세 남자 환자로서 유·소아기에 수차의 후두유두종 수술후에 성대 전연합부에 발생한 격막증에 대하여 금편(gold keel)을 삽입하여 치험하였기에 이를 비데오로 소개하고자 한다.

• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1621-1628
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• 2012

The agar-degrading bacterium GNUM-1 was isolated from the brown algal species Sargassum serratifolium, which was obtained from the West Sea of Korea, by using the selective artificial seawater agar plate. The cells were Gram-negative, $0.5-0.6{\mu}m$ wide and $2.0-2.5{\mu}m$ long curved rods with a single polar flagellum, forming nonpigmented, circular, smooth colonies. Cells grew at $20^{\circ}C-37^{\circ}C$, between pH 5.0 and 9.0, and at 1-10% (w/v) NaCl. The DNA G+C content of the GNUM-1 strain was 45.5 mol%. The 16S rRNA sequence of the GNUM-1 was very similar to those of Alteromonas stellipolaris LMG 21861 (99.86% sequence homology) and Alteromonas addita $R10SW13^T$(99.64% sequence homology), which led us to assign it to the genus Alteromonas. It showed positive activities for agarase, amylase, gelatinase, alkaline phosphatase, esterase (C8), lipase (C14), leucine arylamidase, valine arylamidase, ${\alpha}$-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, ${\alpha}$-galactosidase, ${\beta}$-galactosidase, ${\beta}$-glucosidase, catalase, and urease. It can utilize citrate, malic acid, and trisodium citrate. The major fatty acids were summed feature 3 (21.5%, comprising $C_{16:1}{\omega}7c/iso-C_{15:0}$ 2-OH) and C16:0 (15.04%). On the basis of the variations in many biochemical characteristics, GNUM-1 was considered as unique and thus was named Alteromonas sp. GNUM-1. It produced the highest agarase activity in modified ASW medium containing 0.4% sucrose, but lower activity in rich media despite superior growth, implying that agarase production is tightly regulated and repressed in a rich nutrient condition. The 30 kDa protein with agarase activity was identified by zymography, and this report serves as the very first account of such a protein in the genus Alteromonas.

• Restorative Dentistry and Endodontics
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• 제42권1호
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• pp.54-59
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• 2017

Objectives: The purpose of this study was to compare the antibacterial activity of urushiol against Enterococcus faecalis (E. faecalis) to that of NaOCl. Materials and Methods: The canals of thirty two single rooted human teeth were instrumented with Ni-Ti files (ProTaper Next X1, X2, X3, Dentsply). A pure culture of E. faecalis ATCC 19433 was prepared in sterile brain heart infusion (BHI) broth. The teeth were submerged in the suspension of E. faecalis and were incubated at $37^{\circ}C$ for 7 days to allow biofilm formation. The teeth were randomly divided into three experimental groups according to the irrigant used, and a negative control group where no irrigant was used (n = 8). Group 1 used physiologic normal saline, group 2 used 6% NaOCl, and group 3 used 10 wt% urushiol solution. After canal irrigation, each sample was collected by the sequential placement of 2 sterile paper points (ProTaper NEXT paper points, size X3, Dentsply). Ten-fold serial dilutions on each vials, and 100 µL were cultured on a BHI agar plate for 8 hours, and colony forming unit (CFU) analysis was done. The data were statistically analyzed using Kruskal-Wallis and Mann-whitney U tests. Results: Saline group exhibited no difference in the CFU counts with control group, while NaOCl and urushiol groups showed significantly less CFU counts than saline and control groups (p < 0.05). Conclusions: The result of this study suggests 10% urushiol and 6% NaOCl solution had powerful antibacterial activity against E. faecalis when they were used as root canal irrigants.

• 한국가스학회지
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• 제11권2호통권35호
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• pp.55-59
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• 2007

본 논문은 평판강재를 소성적으로 가공하고, 이것을 용접으로 연결하여 제작한 LPG 용기에 대한 강도 안전성을 고찰하고자 한다. 용기의 강도 안전성은 유한요소해석법을 사용하여 LPG 용기 구조물에서 발생되는 응력분포를 해석함으로써 안전성에 대한 결과를 얻을 수 있다. FEM 해석결과에 의하면, 내압시험을 위한 가스압력 $31kg/cm^2$은 LPG 용기의 상부경판의 프레스 가공 부근에서 국부적인 집중응력이 발생하고, 여기서 발생된 최대응력은 용기 소재의 항복강도를 넘어서는 것으로 나타났다. 따라서 현재의 내압시험 검사방법은 압력용기의 피로손상을 증가시키고 수명을 단축하는 원인으로 작용할 수 있다는 결과를 보여주기 때문에 재검토되어야 하고, 빠른 시일에 개정되어야 할 것이다. 반면에 충전압력 $9kg/cm^2$와 기밀시험 압력 $18.6kg/cm^2$에 의한 용기의 충전과 검사기준에 대한 해석은 LPG 강재용기의 안전성이 비교적 높다는 결과를 제시하고 있다.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2001년도 제3회 부산.경남지부 심포지엄
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• pp.14-28
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• 2001

As basic study for purpose bioremedation in oil-contaminated environment, Primarily, we isolated biosurfactant producer- strains utilized of oil-agar plate, and measured surface tension and emulsifying activity. We investigated in oil-contaminated soil and sea water. In this laboratory, Pseudomonas sp. EL-012S strain isolated from oil-contaminated soil was able to product novel biosurfactant under the optimal culture condition. Its condition was n-hexadecane 2.0%, NH$_4$NO$_3$0.4%, Na$_2$HPO$_4$0.6%, KH$_2$PO$_4$0.4%, MgSO$_4$.7$H_2O$ 0.02%, CaCl$_2$.2$H_2O$ 0.001%, FeSO.7$H_2O$ 0.001%, initial pH 7.0 and aeration at 3$0^{\circ}C$, respectively. This biosurfactant was produced in both late-exponential and early-stationary phase. The biosurfactant from Pseudomonas sp. EL-012S was composed of carbohydrate, lipid and protein. The purified-biosurfactant was examined two (biosurfactant type I, II) with the silica gel G60 column chromatography and the purified biosurfactant confirmed thin layer chromatography, high performed liquid chromatography and gas chromatography. The biosurfactant type I involved in carbohydrate-lipid-protein characteristics lowered surface tension of water to 27dyne/cm and interfacial tension 4.5dyne/cm aginst to n-hexadecane and the biosurfactant type B involved in carbohydrate lipid characteristics lowered surface tension of water to 30dyne/cm and interfacial tension 8dyne/cm against to n-hexadecane. Specially type I had the properties such as strong emulsifying activity, emulsion stability, pH-stability, thermo-stability, high cleaning activity and forming ability.

• 대한영양사협회학술지
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• 제25권1호
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• pp.1-16
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• 2019

This study examined the microbiological quality of daycare center meals. Six menu items from five daycare centers in Daegu included uncooked processed foods (seasoned cucumber and lettuce salad), post-preparation after cooking processed foods (rolled omelet and seasoned soybean sprout), and cooking processed foods (panbroiled beef with oyster sauce and seasoned pork roast). Microbiological analyses were performed for the aerobic plate counts (APC), coliforms, and Escherichia coli. The analyses were conducted in July and November 2017. The mean APC and coliform count of seasoned cucumber decreased significantly from 4.71 log colony forming units (CFU)/g and 2.50 log CFU/g in July to 4.07 log CFU/g and 1.78 log CFU/g in November, respectively (P<0.01 and P<0.001). The APC of panbroiled beef with oyster sauce and seasoned pork roast were significantly lower in July (1.84 and 1.79 log CFU/g) than in November (2.41 and 2.28 log CFU/g) (P<0.001). The coliform counts of panbroiled beef with oyster sauce and seasoned pork roast were significantly greater in November (2.11 and 1.62 log CFU/g) (P<0.001). E. coli was not detected. Among the foods prepared using the three preparation processes, the post-preparation after cooking processed foods had the lowest microbial quality. The APC and coliform counts of cooking processed foods were satisfactory in July, with an acceptable rating for pan-broiled beef with oyster sauce in November. Time-temperature control and the prevention of cross-contamination are essential during meal production for food safety, regardless of the season.