• Title/Summary/Keyword: Plastic tube

Search Result 388, Processing Time 0.023 seconds

Production of Monoclonal Antibody to Human Chorionic Gonadotropin(hCG) : Purification and Properties of a Monoclonal Antibody, and Immunochemiluminometric assay(ICMA) for the Assay of hCG (Human Chorionic Gonadotropin(hCG)에 대한 단일콜론항체 생산 : 단일클론항체의 분리정제 및 그 특성조사와 hCG정량을 위한 Immunochemiluminometric assay(ICMA)개발)

  • 최상훈;이병철;오재욱;이용환;서광영;정길생;김종배
    • Korean Journal of Animal Reproduction
    • /
    • v.12 no.1
    • /
    • pp.51-62
    • /
    • 1988
  • Spleen cells of mouse immunized with hCG were fused with myeloma cell (SP 2/0 Ag 14) to produce monoclonal antibody against hCG. Several clones of hybridoma secreting monoclonal antibody were established and antibodies were characterized in terms of titer, subisotyping and sensitivity in immunoassay. Several methods, for the purification of anti¬bodies, based on gel-filtration, DEAE-ion exchange chromatography and affinity chromatography. were applied and compared each other by the result of SDS-PAGE. Two-site immunochemiluminometric assay (ICMA) involving the use of an excess concentration of a specific monoclonal antibody passively adsorbed onto the walls of plastic tubes and a chemiluminescence labelled antibody conjugate were de¬veloped for the determination of hCG as a preliminary study.

  • PDF

Elasto-plastic time history analysis of an asymmetrical twin-tower rigid-connected structure

  • Wu, Xiaohan;Sun, Yanfei;Rui, Mingzhuo;Yan, Min;Li, Lishu;Liu, Dongze
    • Computers and Concrete
    • /
    • v.12 no.2
    • /
    • pp.211-228
    • /
    • 2013
  • The structure analyzed in this paper has particular building style and special structural system. It is a rigid-connected twin-tower skyscraper with asymmetrical distribution of stiffness and masses in two towers. Because of the different stiffness between the north and the south towers, the torsion seismic vibration is significant. In this paper, in order to study the seismic response of the structure under both frequent low-intensity earthquakes as well as rare earthquakes at the levels of intensity 7, the analysis model is built and analyzed with NosaCAD. NosaCAD is an nonlinear structure analysis software based on second-development of AutoCAD with ObjectARX. It has convenient modeling function, high computational efficiency and diversity post-processing functions. The deformations, forces and damages of the structure are investigated based on the analysis. According to the analysis, there is no damage on the structure under frequent earthquakes, and the structure has sufficient capacity and ductility to resist rare earthquakes. Therefore the structure can reach the goal of no damage under frequent earthquakes and no collapse under rare earthquakes. The deformation of the structure is below the limit in Chinese code. The time sequence and distribution of damages on tubes are reasonable, which can dissipate some dynamic energy. At last, according to forces, load-carrying capacity and damage of elements, there are some suggestions on increasing the reinforcement in the core tube at base and in stiffened stories.

Anti-tumor Effects of Vascular Endothelial Growth Factor Receptor-3 Inhibitor on Oral Cancer Cells (구강암 세포에서 혈관내피성장인자 수용체-3 억제제의 항종양 효과)

  • Kim, Chan-Woo;Kim, Seong-Gon;Park, Young-Wook
    • Maxillofacial Plastic and Reconstructive Surgery
    • /
    • v.34 no.4
    • /
    • pp.239-245
    • /
    • 2012
  • Purpose: Vascular endothelial growth factor (VEGF) plays a key role in tumor angiogenesis and lymphangiogenesis including induction of endothelial cell proliferation, migration and capillary tube formation. E7080 (S1164, Selleck chemical, Houston, TX, USA) is a muti-targeted kinase inhibitor, which targets VEGF receptor-2, 3 (VEGFR-2, 3) and inhibits survival and proliferation of tumor cell. The purpose of this study was to determine the anti-tumor effect of E7080 on oral squamous cell carcinoma. Methods: An oral squamous cell carcinoma cell line, SCC-9 was used in this study. E7080 was applied to SCC-9 cells by 3 different concentrations (1, 5, 10 ${\mu}g/mL$). Control means no application of E7080. The cellular growth was evaluated by real-time cell electronic sensing and MTT assay. The signal transduction was evaluated by Western blotting. Results: In experimental group, SCC-9 cell proliferation was decreased and the VEGFR-3 downstream pathways were inhibited compared with control. Furthermore, increasing the concentration of E7080, the ability of E7080 to disturbance of SCC-9 cell proliferation was increased. Conclusion: Proliferation of SCC-9 cells was inhibited by E7080, which was through by inhibition of VEGFR-3 downstream pathway. In vivo study with E7080 will be required to provide therapeutic benefits in oral squamous cell carcinoma.

VOC Emission Characteristics of Dry Cleaned Wool Scarfs through Small Chamber Test (소형챔버를 이용한 드라이클리닝 모직물 목도리의 휘발성유기화합물 방출 특성)

  • Shin, Jin-ho;Kwon, Seung-mi;Kim, Hyun-soo;Roh, Bang-Sik;Kim, Kwang-rae;Eo, Soo-mi;Jung, Kweon;Lee, Young-Kyu
    • Journal of Environmental Health Sciences
    • /
    • v.43 no.2
    • /
    • pp.95-102
    • /
    • 2017
  • Objectives: This study was conducted to evaluate the emission of VOCs from clothing that had been dry cleaned. Methods: In order to ensure the same conditions, f100% wool scarves were selected as the fabric type. Four identical tests were conducted on the option of either removing the plastic bags which came from the dry cleaning shop or not. The scarf was located inside a closet or room for one or two days. Small chamber tests were conducted to determine the VOC emission characteristics under the same conditions such as temperature, humidity, loading factor, and air exchange rates. Air from the chamber for VOCs was sampled by Tenax TA tube and analyzed by thermal desorption and GC/MSD. Results: Assuming that test represented dry cleaning and consumer's conditions well enough, we can conclude that immediate emissions after the dry cleaning of the scarfs caused elevated levels of TVOC, five VOCs (benzene, toluene, ethylbenzene, xylene, stylene), and decane group compounds. Conclusions: By removing the plastic bags which came from the dry cleaning shop or not, the storage conditions of dry cleaned scarfs by consumers during the storage time periods (one to three days) would be significant for reducing VOC emissions.

Fabrication and Characterization of a Fiber-Optic Alpha/Beta Detector for Nuclear Medicine Application (핵의학 적용을 위한 광섬유 기반의 알파/베타 검출기의 제작 및 특성분석)

  • Hong, Seung-Han;Yoo, Wook-Jae;Shin, Sang-Hun;Seo, Jeong-Ki;Han, Ki-Tek;Jeon, Da-Yeong;Cho, Seung-Hyun;Lee, Bong-Soo
    • Journal of Sensor Science and Technology
    • /
    • v.21 no.5
    • /
    • pp.367-373
    • /
    • 2012
  • We fabricated a fiber-optic alpha/beta detector, which is composed of a sensing probe, a plastic optical fiber, a photomultiplier tube, and a multichannel analyzer, to obtain the energy spectra of radioactive isotopes. As inorganic scintillators of a sensing probe, a ZnS(Ag) film was coupled with a $CaF_2$(Eu) crystal for alpha and beta spectroscopy. In this study, $^{210}Po$ and $^{90}Sr$ were used as alpha and beta sources, respectively, and we measured the radiation energy spectra using a fiber-optic alpha/beta detector to identify alpha and beta emitting radionuclides for nuclear medicine application. Also, the variations of energy spectrum were obtained according to the length of plastic optical fiber.

APICAL SEALING OF THERMAFIL OBTURATORS IN CURVED CANALS (만곡근관에서 Thermafil의 근단폐쇄성에 관한 실험적 연구)

  • Ha, Joo-Hee;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
    • /
    • v.20 no.2
    • /
    • pp.671-684
    • /
    • 1995
  • The purpose of this study was to compare the apical sealing ability, overfilling and obturation of lateral canals using two gutta-percha techniques: Thermafil obturators and cold lateral condensation. Eighty numbered epoxide blocks with one major and five lateral canals were divided into four experimental groups and obturated according to experimental groups. Four experimental groups were as follows: Group 1 : Filling with Thermafil plastic obturators and sealer(ThermaSeal) Group 2 : Filling with Thermafil plastic obturators only, without sealer(ThermaSeal) Group 3 : Fillng using lateral condensation with sealer(AH-26) Group 4 : Fillng using lateral condensation without sealer(AH-26) All the blocks were stored in 100% relative humidor at room temperature for 7 days. Each block was placed in centrifuge tube filled with India ink, and then centrifuged for 20 minutes at 3,000 rpm. Apical leakage was measured from the apical foramen to the most coronal level of dye penetration in millimeter under a stereoscope. The length of gutta-percha and sealer in each of the lateral canals was measured, too. The presence or absence of overfilling of gutta-percha and sealer was recorded. The data was analyzed by one-way ANOVA. The obtained results were as follows: l. Apical dye leakage was almost: not occurred in Group Willed with Thermafil and sealer) and Group 3(filled using lateral condensation with sealer), and there was no significant difference in linear leakage between two groups(p>0.01). 2. In both Thermafil and lateral condensation groups, linear leakage of Group I, 3(filled with sealer) was less than that of Group 2, 4(filled without sealer), and there was no significant difference in linear leakage between Group 1, 2, 3(p> 0.01). 3. Overfilling during obturation of Group 1, 2(filled with Thermafil) was more than Group 3, 4(filled using lateral condensation), and there was no significant difference between groups(p> 0.05). 4. Groups filled with Thermafil had significantly more gutta-percha than groups filled using lateral condensation in all lateral canals(p <0.01), the total length of gutta-percha and sealer found in all lateral canals were similar in Group 1 and Group 3.

  • PDF

EVALUATION OF APICAL PLUG MATERIALS USED FOR THE CONTROL OF EXTRUSION OF HIGH-TEMPERATURE THERMOPLASTICIZED GUTTA-PERCHA (수종의 apical plug 재료의 근단 폐쇄성에 관한 실험적 연구)

  • Hur, Eun-Jung;Choi, Gi-Woon
    • Restorative Dentistry and Endodontics
    • /
    • v.19 no.1
    • /
    • pp.205-216
    • /
    • 1994
  • The purpose of this study is to evaluate of apical plug materials for the contral of extrusion and sealing ability of high-temperature thermoplasticized gutta-percha in plastic root canal blocks. Seventy seven plastic blocks with canal preformed were instrumented with # 50K file 1 mm beyond apical foramen. Blocks were randomly divided into 5 groups of 15 blocks each. Group 1 was filled by high-temperature thermoplasticized gutta-percha only. The another 4 groups were placed with apical plug materials each other and then remaining space was back filled with high temperature thermoplasticized gutta-percha Apical plug materials were used as follows; Group 2: Thermoplasticized gutta-percha (Thermoplasticized gutta-percha group) Group 3 :. Calcium hydroxide powder (Calcium hydroxide group) Group 4 : Silver point (Silver point group) Group 5 : Gutta-percha cone softened by chloroform (Gutta-percha cone group) All the blocks were stored in 100 % relative humidor at room temperature for 14 days. Filling material extruded was removed carefully and then weighed in analytic balance. Each block was placed in centrifuge tube filled with India ink, and then centrifuged for 20 minutes at 3,000 rpm. Apical leakage was measured from the apical foramen to the most coronal level of dye leakage in millimeter by two examiners under a stereoscope. The data were analysed statistically by Student's t-test The obtained results were as follows; 1. The amounts of material extruded through the foramen decreased in all of groups used apical plug materials (P<0.01). 2. Silver point group and gutta-percha cone group were similar linear leakage to high-temperature thermoplasticised gutta-percha technique only (P>0..5). 3. Calcium hydroxide group and thermoplasticized gutta-percha group showed more liner leakage than high-temperature thermoplasticized gutta-percha only (P<0.01, P<0.05). 4. High-temperature thermoplasticized gutta-percha technique with silver point and gutta-percha cone as apical plugs showed less linear leakage and less extrusion of filling material.

  • PDF

A Study on the Water Gas Shift Reaction of RPF Syngas (RPF(Refuse plastic fuel) 합성가스의 수성가스 전환 반응 연구)

  • Roh, Seon Ah
    • Resources Recycling
    • /
    • v.30 no.6
    • /
    • pp.12-18
    • /
    • 2021
  • The water-gas shift reaction is the subsequent step using steam for hydrogen enrichment and H2/CO ratio-controlled syngas from gasification. In this study, a water-gas shift reaction was performed using syngas from an RPF gasification system. The water-gas shift using a catalyst was performed in a laboratory-scale tube reactor with a high temperature shift (HTS) and a low temperature shift (LTS). The effects of the reaction temperature, steam/carbon ratio, and flow rate on H2 production and CO conversion were investigated. The operating temperature was 250-400℃ for the HTS system and 190-220℃ for the LTS system. Steam/carbon ratios were between 1.5 and 3.5, and the composition of reactant was CO : 40 vol%, H2 : 25 vol%, and CO2 : 25 vol%. The CO conversion and H2 production increased as the reaction temperature and steam/carbon ratio increased. The CO conversion and H2 production decreased as the flow rate increased due to reduced retention time in the catalyst bed.

A feasibility study of using a 3D-printed tumor model scintillator to verify the energy absorbed to a tumor

  • Kim, Tae Hoon;Lee, Sangmin;Kim, Dong Geon;Jeong, Jae Young;Yang, Hye Jeong;Schaarschmidt, Thomas;Choi, Sang Hyoun;Cho, Gyu-Seok;Kim, Yong Kyun;Chung, Hyun-Tai
    • Nuclear Engineering and Technology
    • /
    • v.53 no.9
    • /
    • pp.3018-3025
    • /
    • 2021
  • The authors developed a volumetric dosimetry detector system using in-house 3D-printable plastic scintillator resins. Three tumor model scintillators (TMSs) were developed using magnetic resonance images of a tumor. The detector system consisted of a TMS, an optical fiber, a photomultiplier tube, and an electrometer. The background signal, including the Cherenkov lights generated in the optical fiber, was subtracted from the output signal. The system showed 2.1% instability when the TMS was reassembled. The system efficiencies in collecting lights for a given absorbed energy were determined by calibration at a secondary standard dosimetry laboratory (kSSDL) or by calibration using Monte Carlo simulations (ksim). The TMSs were irradiated in a Gamma Knife® IconTM (Elekta AB, Stockholm, Sweden) following a treatment plan. The energies absorbed to the TMSs were measured and compared with a calculated value. While the measured energy determined with kSSDL was (5.84 ± 3.56) % lower than the calculated value, the energy with ksim was (2.00 ± 0.76) % higher. Although the TMS detector system worked reasonably well in measuring the absorbed energy to a tumor, further improvements in the calibration procedure and system stability are needed for the system to be accepted as a quality assurance tool.

Evaluation of the Shielding Effect of Polyvinyl Chloride (PVC) on Low-dose Blending Radiation Energy (폴리 염화 비닐(PVC)의 저선량 융합 방사선에너지에 대한 차폐 효과 평가)

  • Kim, Seon-Chil;Cho, Sung-Hyoun
    • Journal of the Korea Convergence Society
    • /
    • v.10 no.12
    • /
    • pp.129-134
    • /
    • 2019
  • PVC was chosen as a plastic product that can cope with lead, a radiation shielding material that is widely used in medical institutions. In addition to radiation shielding clothing, we want to evaluate whether it can be used as a medical device component and industrial shielding material in low dose areas. Commercial PVC has a density of 3.68 g/㎠ and can be positively expected sufficient shielding effect in certain radiation areas such as material flexibility and economy efficiency, and can be transformed into various forms and used as a lightweight shielding wall. The shielding performance was tested by adjusting the thickness of 5 sheets of 3mm PVC in the range of medical radiation used for clinical examination in medical institutions. Shielding performance against effective energy was evaluated based on tube radiation voltage of medical radiation. The thicker the PVC, the lower the tube voltage and the lower the effective energy, the greater the shielding effect. The shielding effect was 70% at 12mm thickness and 80kVp tube voltage. Therefore, the shielding effect of PVC material has a high dependence of thickness. In the future, continuous research is needed to make thin and light eco-friendly products while improving shielding performance.