• Journal of Nutrition and Health
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• 제25권5호
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• pp.379-388
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• 1992

The present study was designed to evaluate the protective effects of dietary vitamin A or E. and of combination of vitamins A and E on peroxidative deterioration of heart in adriamycin-treated rats. Male Sprague-Dawley rats were assigned to 5 groups according to the dietary supplementation of vitamin A or E Except control rats a dose of 2mg ADR/kg of B. W was injected to these animals intraperitoneally on the same day every week. Adriamycin treatment significantly decreased the weight gain of experimental rats compared with that of control rats, But this decrement was not modified by dietary supplementation of vitamin A or E. Lipid peroxide values of plasma were elevated by ADR treatment. The combined use of ADR and dietary vitamin A or E significantly reduced these values, The interaction between vitamins A and E seemed to be present in the lipid peroxide value of plasma. Catalase and superoxide dismutase(SOD) activities in rat heart were decrased by ADR treatment but glutathione peroxidase(GSH-Px) activity was elevated. Dietary supplmen-tation of vitamin A or E enhanced the heart catalase and SOD activities. except only vitamin A-supplemented group. GSH-Px activity of rat heart tended to be decreased by dietary supple-mentation of vitamin A or E. With ADR treatment polyunsaturated fatty acids such as archido-nic acid(20:4) and docosahexaenoic acid(22:6) were decreased in rat heart. However dietary supplementation of vitamins A and E reduced this decrease. The retinol and tocopherol contents of rat plasma were decreased by ADR treatment. Dietary vitamin A or E influence vitamin A or E content of plasma. The interaction between dietary vitamins A and E was observed in vitamin A or E level of rat plasma.

• 한국자원식물학회지
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• 제18권2호
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• pp.343-350
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• 2005

미나리즙이 과산화지질과 알코올을 급여한 흰쥐의 지질구성, 간장기능과 항산화능에 미치는 영향을 검토했다. 혈장 총콜레스테롤량은 과산화지질과 알코올을 급여한 처리군 모두가 정상군보다 증가하였다. 그러나 미나리즙 처리군들은 대조군보다 낮았다. 혈장HDL-cholesterol량은 미나리즙 처리군들이 대조군에 비하여 높은 값을 나타냈으며, 정상군과 유의한 차이를 나타내지 않았다. 혈장 Triglyceride량은 과산화지질과 알코올을 급여한 처리군 모두가 정상군 보다 증가하였으나, 미나리즙을 처리한 군이 대조군 보다 낮은 값을 보여, 하락하는 경향을 보였다. 간장의 총콜레스테롤량 및 Triglceride량은 대조군이 가장 높은 값을 나타내었으며, 정상군과 미나리즙 처리군간에는 유의한 차이를 나타내지 않았다. 혈장 및 간장의 TBARS량은 정상군과 미나리즙 처리군은 유의한 차이를 나타내지 않았으며, 대조군 보다 낮은 수치를 보였다. 혈장 GOT 및 GPT의 활성치는 과산화지질과 알코올의 급여에 의해 증가하였으나, 미나리즙 처리에 의해 하락했다(P<0.05). 간장 GSH-Px, SOD 및 CAT활성치는 미나리즙 처리에 의해 증가하였다.

• Journal of Animal Science and Technology
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• 제48권6호
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• pp.897-906
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• 2006

본 연구는 셀레늄강화 한우고기 생산을 위하여 셀레늄강화 버섯폐배지(Se-SMC)가 함유된 셀레늄사료(사료내 셀레늄함량: 0.9ppm)를 활용하여 급여사양기간(2개월, 3개월, 4개월)을 달리 하였을 때, 비육후기 거세한우의 조직 내 셀레늄 함량과 혈중 글루타치온 과산화효소(GSH-Px) 활성에 미치는 영향을 조사하여 최대 포화축적기간을 제시하고자 실시하였다. 본 연구에 사용된 비육후기 거세한우는 30두로 각 급여기간(2개월: 평균 체중 677kg, 3개월: 평균 체중 610kg 및 4개월: 평균 체중 524kg)에 대한 대조구 및 Se-SMC 급여구로 나누어 처리구당 5두씩 배치하여 총 6처리구로 구분하여 실시하였다. 대조구와 Se-SMC 급여구에 사용된 실험사료는 0.1과 0.9ppm의 셀레늄을 각각 함유하였다. 각 급여기간이 종료되면 도축하여 후지와 간을 채취하였으며 이를 셀레늄 함량분석에 이용하였다. 그리고 혈중 셀레늄 농도 및 글루타치온 과산화효소활성을 분석하기 위하여 도축 시에 채혈을 실시하였다. 건물섭취량은 Se-SMC 급여 및 급여기간에 의하여 유의한 차이가 나타나지 않았다. 또한 일당증체량은 대조구 및 Se-SMC 급여구간 그리고 급여기간 간에 유의한 차이가 나타나지 않았을 뿐만 아니라 총증체량 또한 각 급여기간내 대조구와 Se-SMC 급여구 간에 유의한 차이가 나타나지 않았다. Se-SMC 급여는 혈중 셀레늄농도를 유의하게 증가시켰으나(P<0.001), 급여기간에 따른 혈중셀레늄 농도에는 유의한 영향을 미치지 않았다. 아울러 혈중 GSH-Px 활성은 혈중 셀레늄농도에서 나타난 양상과 유사한 경향을 나타내었고, 급여기간에 의한 효과는 나타나지 않았다. 후지내 셀레늄 함량은 급여기간(P<0.05)이 증가함에 따라, 그리고 Se-SMC 급여(P<0.001)에 의하여 유의하게 증가하였다. 하지만, Se-SMC의 3개월 급여군과 4개월 급여군 간에는 유의한 차이가 나타나지 않았다. 간내 셀레늄 함량은 Se-SMC의 급여기간이 증가함에 따라 다소 증가하는 경향을 나타내었으나 유의한 효과는 나타나지 않았다. 하지만, 동일한 급여기간 내 Se-SMC는 대조구에 비하여 유의하게 높았다(P<0.01).이상의 결과로부터 근육내 셀레늄 축적을 위한 적정 Se-SMC 급여기간은 후지와 간내 셀레늄 함량을 고려할 때 약 2 내지 3개월의 급여가 충분할 것으로 사료된다.

• 한국자원식물학회지
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• 제26권2호
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• pp.227-233
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• 2013

본 연구는 산마늘추출물이 과산화지질을 급여한 비만쥐의 지질강하, 항산화효과 및 염증매개물질의 생산에 미치는 영향을 검토했다. 그 결과 혈장 FFA, TG, total cholesterol 및 LDL-cholesterol 농도는 산마늘추출물 처리군 들에서 감소했으며, 혈장HDL-cholesterol 농도는 산마늘추출물 처리군 들에서 증가했다. 간장 내 total cholesterol 농도 및 TG 농도는 산마늘추출물 처리군 들에서 감소하는 경향을 나타내었다. 혈장 및 간장의 TBARS 농도는 산마늘추출물 처리군 모두가 대조군보다 낮은 경향을 보였다. 간장 GSH-Px, SOD 및 CAT활성치모두가 산마늘추출물 처리군 들에서 증가하는 경향을 보였다. 혈장 NO, Ceruloplasmin 및 ${\alpha}1$-acid glycoprotein 농도는 산마늘추출물 투여군 들이 대조군보다 낮은 경향을 나타내었다. 이와 같은 결과는 산마늘추출물에 지질강하, 항산화 및 항염증작용에 효과를 나타내는 기능성물질이 내재하고 있음을 시사한다.

• 대한지역사회영양학회지
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• 제7권6호
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• pp.844-851
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• 2002

Smoking can increase oxidative stress and thereby change the antioxidant defense system in the body. To investigate the relationship between male adolescent smoking and antioxidant status, we surveyed the eating habits and dietary intake of 82 smokers and 44 nonsmokers recruited from a male technical high school. In addition, antioxidant enzyme activity and lipid peroxide values were determined in both the plasma and the erythrocytes. Although the frequency of food intake was not significantly different, most nutrient intake was unexpectedly higher in smokers than in nonsmokers. In comparison with the Korean RDA, especially the average intake of Ca, Fe and vitamin $B_2$ didn t reach 75% of the Korean RDA in either smokers or nonsmokers. An analysis of antioxidant enzyme activity showed that plasma catalase. superoxide dismutase (SOD), glutathione peroxidase (GSH-px), erythrocyte catalase and GSH-px activities showed no significant difference between smokers and nonsmokers. However, the erythrocyte SOD activity of smokers (1.57 unit/mgHb) was significantly lower than that of nonsmokers (2.00 unit/mg Hb). In addition, the plasma ceruloplasmin concentration of smokers (28.68 mg/$d\ell$) was significantly higher than that of nonsmokers (26.30 mg/$d\ell$), whereas the specific ceruloplasmin ferroxidase activity of smokers (0.31 unit/mg) was lower than that of nonsmokers (0.35 unit/mg). The plasma and erythrocyte thlobarbituric acid reactive substance (TBARS) of smokers (2.57 $\mu$mol/L, 0.32 $\mu$mol/gHb) were also significantly higher than those of nonsmokers (2.25 $\mu$mol/L, 0.27 $\mu$mol/gHb). The overall data indicate that adolescent smoking might decrease the antioxidant capacity of the body, in part, by lowering the erythrocyte SOD activity and the specific ceruloplasmin ferroxidase activity.

• 한국자원식물학회지
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• 제28권5호
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• pp.600-607
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• 2015

본 연구는 우슬과 골쇄보 활용하여 in vitro와 in vivo에 대한 실험으로 진행 되었다. 이 두 가지 약용작물을 추출하고 골다공증을 예방하는 능력을 연구하였다. 연구재료로 사용 된 시료는 증류수를 사용하여 추출 후 동결건조 하였다. 시료는 다시 다이메틸설폭사이드에 녹여서 사용 하였다. in vitro실험의 스텐다드 프로토콜에 맞추어 DPPH 라디칼 소거능, 환원력, 총항산화력, SOD 유사활성 그리고 Fe²⁺chelate활성을 측정 하였다. In vivo동물 실험에서는 난소를 제거한 스프레그다우리 쥐를 4개의 식이요법그룹으로 무작위로 나누었다. SHAM: 모의처치 대조군, OVX: 난소를 제거한 후 일반 식이를 먹인 쥐, OVX-AR: 난소를 제거한 후 우슬을 먹인 쥐, OVX- DR; 난소를 제거한 후 골쇄보를 먹인 쥐, 사용하였으며 그리고 8주 동안 실험 식이를 먹인 후 혈장 및 적혈구 TBARS 농도와 SOD, GSH-Px, GR, CAT 활성도를 측정하였다. in vitro항산화 활성 분석 결과를 보면 양성 대조군이 1% Vitamin C 용액이 94%정도의 DPPH 라디칼 소거능을 보였으며 우슬과 골쇄보 추출물에 대한 DPPH 값은 골쇄보의 값이 우슬보다 유의적으로 높은 소거능을 보였다. 환원력에서도 골쇄보가 우슬보다 유의적으로 높은 높은 값을 보였다. 반면에 총 항산화력에서는 우슬이 골쇄보다 유의적으로 높은 항산화력을 나타내었다. SOD의 측정값은 우슬과 골쇄보 간에 유의적 차이가 없었다. Fe²⁺ chelate활성에선 DPPH와 환원력과 달리 우슬이 77.60%로 골쇄보 46.21%에 비해 유의적으로 높은 활성도를 나타내었다. 혈장 TBARS 농도는 우슬 추출물을 섭취한 OVX-AR군이 13.58 ± 0.20 (nmol/㎖)로 SHAM군의 15.70 ± 0.20와 비슷한 수준으로 낮았고, 난소 제거 후 일반 식이를 먹인 OVX군이 20.70 ± 0.08로 유의적으로 가장 높은 수치를 나타 내어 과산화 지질 함량이 가장 높았다. 간 조직 항산화효소 활성을 보면 OVX- AR군(우슬)이 GSH-Px, PON에서 유의적으로 높은 활성을 보였고 CAT와 GR에서 OVX-DR군(골쇄보)이 유의적으로 높게 났다. 신장 조직 항산화 효소 활성에서는 OVX- DR군(골쇄보) 이 SOD, GSH-Px, GR, PON 에서 유의적으로 높게 나타났으며, 자원 추출물 간의 CAT 활성도의 유의적인 차이는 없었다. 적혈구 항산화 효소 활성은 SOD에서 OVX-DR군(골쇄보)이, GSH- Px에서는 OVX-AR 군(우슬)이 유의적으로 높았고, CAT와 GR 활성은 난소절제 후 OVX(자원식물 추출)군이 SHAM(일반식이 투여)군 보다 유의적으로 높은 항산화효소 활성을 보여 주었다.

• 대한한방소아과학회지
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• 제19권1호
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• pp.153-171
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• 2005

Objective : This experiment has been done to evaluate the effects of Gamisogunjung-tang(GST) on antioxidant capability and lipidic concentration in blood which are presumed to be related to aging. Method : In this study, we divided 14 weeks old SD rats into normal group, control group and GST group. Control and GST group were induced aging by D-galactose. At the same time GST group were administered extract of Gamisogunjung-tang for 6 weeks. After then we took blood, and measured the activities of SOD, GSH-px, catalase in erythrocytes and measured TBARS values, concentrations of total lipid, tryglyceride, total cholesterol, HDL-cholesterol in plasma. Results : The activities of SOD, GSH-px, catalase in erythrocytes increased significantly in GST group compared with control group. The value of TBARS and the concentration of total lipid, total cholesterol in plasma decreased significantly in GST group compared with control group. The concentration of HDL-cholesterol increased significantly in GST group compared with control group. The concentration of triglyceride were not noticeable. Conclusion : it is considered that Gamisogunjung-tang has an influence on control aging by activation the antioxidative enzyme systems in erythrocytes and decreasing the concentration of lipid in blood plasma.

• Journal of Nutrition and Health
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• 제32권1호
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• pp.17-23
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• 1999

The purpose of this study was to determine the extent of oxidative stress in NIDDM patients with diabetic complications and to determine the relationship between oxidative stress and diabetic complications. For this study, 139 NIDDM patients were recruited, 85 with diabetic complications and 54 without complications were recruited. The concentration of malondialdehyde(MDA) and the activities of antioxidant enzymes including catalase, superoxide dismutase(SOD), gluthatione peroxidase(GSH-Px)were determined. The daily intakes and plasma concentrations of beta-carotene, lycopene, lutein nd alpha-tocopherol were determined by food frequency questionnaire and by high performance liquid chromatography(HPLC), respectively. Among the antioxidant enzymes studied, only GSH-Px activity was lower in NIDDM patient, with diabetic complications than in those without complications(2.91$\pm$0.80 vs 3.54$\pm$0.44 U/mgHb, p<0.05). Those NIDDM patients with diabetic complications had higher MDA concentrations than those without diabetic complications(1.40$\pm$0.25 vs 1.25$\pm$0.11 nmol/ml, p<0.05). There were no significant differences in the dietary intakes of total carotenoids(2854 vs 2824ug/day)or vitamin E (9.5$\pm$3.2 vs 9.5$\pm$2.0mg/day)between NIDDA patients with and without complications. However, the plasma concentrations of beta-carotene and lycopene were significantly lower in NIDDM patients with complications than in NIDDM patients without complications (Beta-carotene : 24.2$\pm$12.5 vs 33.1$\pm$16.2(ug/dl), lycopene : 2.8$\pm$2.1 vs 4.3$\pm$2.8(ug/dl)). This study showed that in NIDDM patients with complications, the lipid peroxidation of erythrocytes was higher increased and the antioxidant reserves were significantly dipleted, compared with NIDDM patients without complications. The lower plasma concentrations of beta-carotene and lycopene in NIDDM patients may be due to the presence of diabetic complication, not due to the lower dietary intakes of antioxidant vitamins. To define the role of carotenoids in diabetes, more experimental and clinical studies are needed.

• Korean Journal of Acupuncture
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• 제26권2호
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• pp.53-60
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• 2009

Objectives: To investigate the effects of Hominis placenta pharmacopuncture on the blood picture and antioxidative activity in rats. Methods: Sprague-Dawley rats were divided into 3 groups; normal control (n=5), pharmacopuncture at CV12 (CV12 group, n=5), and pharmacopuncture at ST36 (ST36 group, n=5) once every other day for 4 weeks. Blood cell counting was performed and liver superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities were analyzed. Results: Values of red blood cell and plasma cell volume were significantly higher in the ST36 group than the normal control. Values of hematocrit, total protein, and albumin were not significantly different among groups. White blood cell count and the percentage of neutrophils, lymphocytes, and eosinophils were not significantly different among groups. However, monocytes and basophils were significantly increased in the ST36, and CV12 groups, respectively. SOD and CAT in the CV12 and ST36 groups were significantly activated than in the normal control group, while the activity of GSH-Px showed no significant difference among groups. Conclusions: Based on the findings of this study, Hominis placenta pharmacopuncture may have positive impact on antioxidative capacity, thus activate various functions of the body.

• 한방재활의학과학회지
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• 제21권1호
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• pp.23-33
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• 2011

Objectives : The present study investigated effects of Artemisia Capillaris Thunberg ethanol extract(EtOH ext). on lowering lipid, anti-oxidation and concentration of plasma inflammatory mediators using rat fed on high oxidized fat. Methods : We divided fat sprague-dawley rats fed on high oxidized into 4 groups. They were normal group, feed with 100 mg/kg Artemisia Capillaris Thunberg group, feed with 200 mg/kg Artemisia Capillaris Thunberg group and feed with 300 mg/kg Artemisia capilaris Thunberg group. They were administered for 4 weeks. We measured concentration of plasma free fatty acid(FFA), plasma triglyceride, plasma total cholesterol, and plasma low density lipoprotein-cholesterol(LDL-cholesterol), plasma high density lipoprotein-cholesterol(HDL-cholesterol), concentration of liver total cholesterol and liver triglyceride (TG), concentration of plasma thiobarbituric acid reactive substance(TBARS) and liver thiobarbituric acid reactive substance(TBARS), glutathione peroxidase (GSH-Px) activity, superoxide dismutase(SOD) activity and catalase(CAT) activity, plasma nitric oxide(NO), ceruloplasmin and ${\alpha}-glycoprotein$. Results : 1. The Artemisia Capillaris Thunberg EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL-cholesterol was increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 2. Concentration of liver total cholesterol and liver TG showed a significantly decrement in all Artemisia Capillaris Thunberg EtOH ext. groups than that of control group. 3. The Artemisia Capillaris Thunberg EtOH ext. groups showed lower values in concentration of plasma TBARS and liver TBARS than that of control group. The values of GSH-Px activity, SOD activity and CAT activity were increased in the Artemisia Capillaris Thunberg EtOH ext. groups. 4. The values of plasma NO, ceruloplasmin and ${\alpha}-glycoprotein$ were decreased in Artemisia Capillaris Thunberg EtOH ext. groups. Conclusions : Based on the results in this study, the Artemisia Capillaris Thunberg EtOH ext. showed a positive effect in lowering lipid, anti-oxidation and decrement of plasma inflammatory mediators.