• 미생물학회지
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• 제27권4호
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• pp.430-435
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• 1989

Dextranase와 glucose←oxidase의 향치아우식 인자로서의 효괴와 dentifrice 앙분으로서의 이용 가능성융,경토하였다. W Water-insoluble glucan에 의한 plaque의 형성은 dextranase와 glucose-oxida앤플 사용함으호써 억제할 수 9.]였다. D Dextranase의 경우 낮은 농도에서도 plaque의 형생억저l와 분해에 매우 효과적이였다. Glucose-oxidase의 경우 얄균작용 에 의해 생균수를 줄임으로써 plaque의 형성억제에는 효과적이었으나 분해작용은 미약하였다. Dentifrice의 각 성분에 대한 compatibility test 를 통해 효소의 안쟁화를 위한 Mode] Dentifrice 플 구생하였고. Model Dentifrice 에서의 activity 변 화플 관창한 결과 안정성이 오랫동안 유지될을 확인할 수 있었다.

• 치위생과학회지
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• 제14권2호
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• pp.87-93
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• 2014

Dental plaque resides passively at a site and makes an active contribution to the maintenance of health. The bacterial composition of plaque remains relatively stable despite regular exposure to minor environmental stress. This stability, homeostasis is due to a dynamic balance of microbial interactions. However, the homeostasis can break down, leading to shifts in the balance of the microflora. This change can be a sign of initial dental caries. It is proposed that disease can be prevented or treated not only by targeting the putative pathogens but also by interfering with the processes that drive the breakdown in homeostasis. It is essential to understand the plaque as a mixed species biofilm. In this essay I reviewed an extension of the caries ecological hypothesis to explain the relation between dynamic changes in the phenotypic/genotypic properties of plaque bacteria and the demineralization and remineralization balance of the dental caries process. We will have the strategies to impact significantly on clinical practice as understanding dental biofilm.

• 미생물학회지
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• 제32권1호
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• pp.60-64
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• 1994

자연계로부터 식물병원균인 Pseudomonas syringae pv. Tabaci에 감염하는 bacteriophage 를 분리하였다. 이 phage의 안정성을 조사한 결과 중성 부근 pH에서 가장 안정하였고 50${\circ}C$ 이상에서는 안정성이 급격히 감소하였으며 흡착시간 별로는 10분까지는 빠른 흡착율을 보이다가 그 후부터는 서서히 감소하였다. 또한 흡착에는 금속이온을 필요로 하였으며 흡착온도는 20${\circ}C$에서 가장 높게 나타났고 20~40분에서 가장 높게 나타났다. 또한 배양온도에 따라 plaque 양상이 달랐는데 10${\circ}C$에서는 clear plaque를, 20, 30${\circ}C$에서는 turbid plaque를 형성하였다. 20${\circ}C$에서 잠복기는 약 3시간이었고 평균 방출수는 200PFU/cell이었다. 유전물질로는 ds DNA를 가지고 있었고 크기는 30kb 정도이었다.

• 한국치위생학회지
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• 제10권2호
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• pp.311-322
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• 2010

Objectives : Since the program about sodium lauryl sulfate that might cause oral dryness and taste change including oral tissue allergy was on the air, the ingredients of D.I.Y dentifrices without sodium lauryl sulfate have been sold in online shopping mall and ordinary people can make the dentifrices easily. But there have not been any reports about the effect of dental plaque removal and preference about D.I.Y dentifrices. Therefore, this study was designed as a pilot study which aimed to investigate the effect of dental plaque removal and prefernece about D.I.Y dentifrices. Methods : 6 subjects were collected to test the effect of dental plaque removal with D.I.Y dentifrices with written consent. They didn't brushed the teeth during 12 hours until the check-up time on the next day. The O'leary index was calculated in baseline, 1 minute, 2 minute, 3 minute after brusing with D.I.Y dentifrices and market dentifrices. The preference about D.I.Y dentifrices through self-administered questionnaire was surveyed in 51 subjects after using D.I.Y dentifrices. Results : 1. The market dentifrices could remove larger amount of dental plaque than D.I.Y dentifrices, but, it wasn't significant. 2. In feeling refreshment, the market dentifrices had more positive answers significantly. 3. In feeling taste change, the market dentifries had more duration of taste change significantly. 4. In feeling oral dryness, the market dentifrices had more duration of oral dryness after toothbrushing. but, it wasn't significant. 5. As a result about reviewing the dental journals about ingredients of D.I.Y dentirices, green tea, sodium carbonate, bamboo salt, propolis had each evidence. But, We could not find out the evidences of calculus adhesion by corn starch, preservative by napri, disinfectant of peppermint. Conclusions : Although we cannot find the difference of the effect of dental plaque removal between D.I.Y dentifrices and market dentifrices, and D.I.Y dentifrices have the merits of decrease of oral dryness and taste change, it was suggested to have another test about stability and safety of D.I.Y dentifrices for safety of the user of D.I.Y dentifrices.

• 구강회복응용과학지
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• 제18권2호
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• pp.119-126
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• 2002

The purpose of this study was to restorate a patient who has a few remaining teeth with #15,23,24 supported Konus telescope denture in Maxillar and #44,43,33,34 supported Dolder bar retained overdenture in Mandible. Konus telescope and bar retained overdenture was taken better results in retention, support, stability compair with regular Removable partial denture. In Removable partial denture, the change of remaining teeth and edentulous ridge is natural. But Konus telescope and bar retained overdenture is a little effected in this change, so it is possiblble in long-term use. In cosider of patient's medical history and the possibility of additional tooth loss, Konus telescope denture can be easily repaired. Compaired with Konus telescope and bar retained overdenture showed high stability and easy cleansing because of rigid support, cross - arch splinting, and simple design. In delivery, patient had a difficulty with removal of denture and plaque control, but showed better condition, good oral hygienic care. Patient satisfied with denture functionally and esthetically. This study showed Konus telescope and bar retained overdenture was effective for treatment of patient remaing a few teeth in function, esthetic and psycologic satisfaction.

• 미생물학회지
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• 제24권2호
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• pp.161-167
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• 1986

Some of the physiological properties of Sp816 bacteriophage of Bacillus subtilis SNU816 were characterized. It could form plaques on either B. subtilis SNU816 or B. natto 8102, but not on any other bacillus strains investrgated. Its plaque morphology was circular with a diameter of less than 1.0mm and had a narrow halo surrounding the clear center. Its latent period was 34-36 min and had a burst size of 547. It was most stable at pH 6.0, and rapidly inactivated at $60^{\circ}C$ with a initial deaty rate of -0.216 $min^{-1}$. Host range, thermal inactivation rate at $60^{\circ}C$, pH stability, and UV sensitivity revealed that SP816 was quite different from any other phages investigated together but seemed to be rather related to B. natto phages.

• The Journal of Advanced Prosthodontics
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• 제3권1호
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• pp.20-24
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• 2011

PURPOSE. The aim of this study was to identify in vitro antimicrobial activity of the tissue conditioner containing silver nanoparticles on microbial strains, Staphylococcus aureus, Streptococcus mutans and Candida albicans. MATERIALS AND METHODS. Experimental disc samples ($20.0{\times}3.0$ mm) of tissue conditioner (GC Soft-Liner, GC cooperation, Tokyo, Japan) containing 0.1 - 3.0% silver nanoparticles (0%: control) were fabricated. Samples were placed on separate culture plate dish and microbial suspensions (100 ${\mu}L$) of tested strains were inoculated then incubated at $37^{\circ}C$. Microbial growth was verified at 24 hrs and 72 hrs and the antimicrobial effects of samples were evaluated as a percentage of viable cells in withdrawn suspension (100 ${\mu}L$). Data were recorded as the mean of three colony forming unit (CFU) numerations and the borderline of the antimicrobial effect was determined at 0.1% viable cells. RESULTS. A 0.1% silver nanoparticles combined to tissue conditioner displayed minimal bactericidal effect against Staphylococcus aureus and Streptococcus mutans strains, a 0.5% for fungal strain. Control group did not show any microbial inhibitory effect and there were no statistical difference between 24 hrs and extended 72 hrs incubation time (P > .05). CONCLUSION. Within the limitation of this in vitro study, the results suggest that the tissue conditioner containing silver nanoparticles could be an antimicrobial dental material in denture plaque control. Further mechanical stability and toxicity studies are still required.

• 미생물학회지
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• 제23권2호
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• pp.107-114
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• 1985

우리나라 자연환경으로부터 분리한 Rhizob ium 32권주로부터 카나마이신, 세트라싸이클린 등에는 예민하고, 클로람페니콜, 젠타마이신 등에는 강한 내성을 나타내는 5 균주를 선별하고, 이를 수용세포로하여 RP4::Mu cts를 접함에 의해 E. coli로부터 Rhizobium leguminosarum으로 전달 시켰다. 그 전달빈도는 $5.8{\times}10^{-7}$의 빈도를 나타내었다. 접합체에서의 RP4::Mu cts플라스미드의 존재는 암피실린과 카나마이신, 테트라싸이클린에 대한 내정과 $42^{\circ}C$에서의 플라크 형성으로 확인하였다. 접합체들은 $10^2~10^3$단위로 플라크를 형성하였고, 안정성를 조사한 신파 4주 후에도 대부분이 RP4: :Mu cts의 성질을 유지하고 있는 것으로 나타났다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제48권4호
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• pp.225-231
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• 2022

Objectives: This study aims to evaluate the efficacy and safety of two types of sandblasted with large-grit and acid-etched (SLA) surface implants with different surface roughness. Patients and Methods: This study was conducted based on a clinical record review of 55 patients (mean age, 53.00 years). A total of 80 SLA surface implants was placed. Among the 80 implants, 38 implants placed in 29 subjects had surface roughness (Ra) of 3.09 ㎛ (test group, TG), while the other 42 implants placed in 31 subjects had a surface roughness (Ra) of 2.50 ㎛ (control group, CG). A comparison was made of implant primary/secondary stability; success and survival rates; marginal bone loss; and soft tissue assessment including probing pocket depth (PPD), plaque index (PI), gingival index (GI), and bleeding on probing (BOP) between the groups at 1 year after implant placement. Results: Among the implants that were initially registered, 1 from the TG and 4 from the CG dropped out, leaving 37 implants in the TG and 38 implants in the CG to be traced and analyzed. Although 1 TG case showed unstable primary stability, all cases showed stable secondary stability. Success and survival rates at 1 year after implant placement were 100% in both groups. Marginal bone loss was 0.07 mm and 0.00 mm for the TG and CG, respectively, but the difference was not significant. Among the several parameters for evaluation of soft tissue, the TG showed lower PI at 1 year after implant placement (TG=0.00, CG=0.29; P=0.0004), while the remaining categories showed no significant difference between the groups. Conclusion: This study shows that the two types of SLA implants with different surface roughness have no difference in efficacy or safety. Therefore, both of the implants can be used safely and with promising outcomes.

• Journal of Periodontal and Implant Science
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• 제37권3호
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• pp.625-636
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• 2007

Marginal tissue recession makes problems like esthetics, root caries, hypersensitivity and plaque accumulation. Request for root coverage is higer than ever, especially esthetic problems involved. So techniques for root coverage hav been developed. There are some kinds of surgical techniques using soft tissue for root coverage. For example, free gingival graft, kinds of pedicle flap, subepithelial connective tissue graft(SCTG), and so on. Subepithelial connective tissue graft has many advantage for root coverage, that is less pain on donor site, good blood supply for graft, and more esthetic result. For this reaseon, this case report was performed to evaluate the effect of root coverage using subepithelial connective tissue graft. Three patients has Miller's class I marginal tissue recession and one patients has Miller's class III marginal tissue recession. The following period is 36.5 month on average. The results are as follows: 1. Root coverage of 100% was obtained in 5 of 6 defects, and 80% was obtained in 1 of 6 defects, The mean root coverage was 96,6% in six cases on 4 patients. 2. The mean root coverage was 3.83mm and mean recession depth decreased from 4mm to 0.16mm. 3. The mean width of clinical attached gingiva increased from 1.5mm to 4mm. The mean width of gained attached gingiva after surgery was 2.5mm. 4. The mean follow up period was 36.5 months. The longest follow up period was 50 months and the shortest follow up period was 22 months. 5. The result that obtained by surgery was stable during follow up period. Within the above results, root coverage with SCTG is an effective procedure to cover marginal tissue recession defect with long term stability.