• Journal of the Korean Society of Tobacco Science
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• v.15 no.2
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• pp.137-144
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• 1993

The present study was conducted to compare the efficiency of individual plant selection for resistance to bacterial wilt with 30 dihaploid lines derived by anther culture and Nicotiana africana method in Fl and F2 generation from a cross between Bright Yellow 4 (BY4) and NC95. F2 dihaploid lines were selected from bacterial wilt disease resistant plants screened under the naturally infested filed conditions. The populations of FB - ADH and FB MDH derived from F2 individual plants with bacterial wilt resistance showed higher resistance to the disease than the populations of Fl - ADH and Fl - MDH, respectively, and no difference for the disease resistance appeared by the haploid deriving method within a generation.

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.131-134
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• 1998

$\textrm{T}_{5}$ progeny of one transgenic tomato line (To9) carrying antisense polygalacturonase (PG) cDNA was generated by selfing. Five $\textrm{T}_{5}$ plants were used to analyse in detail. The PG antisense gene was stably inherited through fifth generations. In all five $\textrm{T}_{5}$ plants, expression of the antisense transcripts were detected. In consequence, it led to a reduction of the PG enzyme activity in ripe fruit to between 37% and 65% that of normal. In two plants the expression of endogenous PG gene was inhibited in ripe fruit.

• KSBB Journal
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• v.4 no.3
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• pp.246-253
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• 1989

Hairy roots of carrot were induced by Agrobacterium rhizogenes $A_4$ strain within about 2-4 weeks after inoculated from root disc. Early axonic culture is established in RCM agar medium and following is in MS rigid medium. After 15 days culture, the hairy roots were vigrous growth in about 10 times of initial inoculum. Anthocyanin contents of hairy roots were more than of ordinary roots. 2, 4-D ($10^{-4}mg/ l$), sucrose (5%), nitrogen source (0.03M) contained medium was optimized to growth of hairy root and contents of anthocyanin. Phenotypic alterations of leaves are observed in transformed plants and determined the transformation of hairy roots and the transformed plants by opine assay.

• The Journal of Natural Sciences
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• v.6 no.1
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• pp.71-78
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• 1993

Five $F_1$ hybrids of radish(Raphanus sativus L.) were used in the study for induction of microspore derived embryos. Anthers from the mid-uninucleate to early bicellular stage were inoculated on the modified B5 medium and modified Nitch-Nitch medium supplemented with several growth regulators. The efficiency of anther culture was dspendent on the genotype of donor plants and we obtained various culture efficiency from different genotypes. Induction of embryos from microspore was best result on Nitsch-Nitsch media supplemented with 0.1mg/l NAA and 0.05mg/l BAP. Heat treatments of anthers at $35^{\circ}C$-2days and combined with pretreatment of $4^{\circ}C$ for 2, 8, 12 and 16days . Among the treatments, $35^{\circ}C$-2 days treatment combined with $4^{\circ}C$-2days pretreatment treatment were the most effective in developing embryos from microspores.

• Mycobiology
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• v.45 no.4
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• pp.401-408
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• 2017

Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 $(GT)_n$, CG2 $(GT1)_n$, CG3 $(TC)_n$, CG4 $(CT)_n$, and CG5 $(CT1)_n$ were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism. The selected microsatellites were used to analyze 31 strains of C. gloeosporioides isolated from 20 different host plants from India. All microsatellite loci were found to be polymorphic, and the approximate fragment sizes of microsatellite loci CG1, CG2, CG3, CG4, and CG5 were in ranges of 213-241, 197-227, 231-265, 209-275, and 132-188, respectively. Among the 31 isolates, 55 different genotypes were identified. The Simpson's index of diversity (D) values for the individual locus ranged from 0.79 to 0.92, with the D value of all combined five microsatellite loci being 0.99. Microsatellite data analysis revealed that isolates from Ocimum sanctum, Capsicum annuum (chili pepper), and Mangifera indica (mango) formed distinct clusters, therefore exhibited some level of correlation between certain genotypes and host. The developed MLMT method would be a powerful tool for studying the genetic diversity and any possible genotype-host correlation in C. gloeosporioides.

• Korean Journal of Plant Tissue Culture
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• v.21 no.5
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• pp.315-318
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• 1994

To obtain transformed plants, we cocultured cotyledonary explants of Codonopsis lanceolata with Agrobacterium tumefaciens LBA4404, a disamed strain harboring a binary vector pBI121 carrying the CaMV35S promoter-$\beta$-glucuronidase (GUS) gene fusion used as a reporter gene and NOS promoter-neomycin phosphotransferase gene as a positive selection marker in MS liquid medium with 1mg/L BA. After 48 h of culture, explants were transferred onto MS solid medium with Img/L BA, 250mg/L carbenicillin, and 100mg/L kanamycin sulfate and cultured in the dark. Numerous adventitious buds formed on the cut edges of the explants after 2 weeks of culture. When subjected to GUS histochemical assay buds showed a positive response at a frequency of 15%. Explants formed adventitious shoot at a frequency of 56.7%, after 6 weeks of culture. Upon transfer onto the basal medium, most of the shoots were rooted and subsequently the regenerants were transplanted to potting soil. Southern blot analysis confirmed that the GUS gene was incorporated into the genomic DNA of the GUS-positive regenerants.

• The Journal of the Convergence on Culture Technology
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• v.10 no.3
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• pp.559-564
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• 2024

Since the era of industrial revolution, the waste volume has continued to grow; waste management has thus become one of the major global environmental challenges facing humanities today. Waste treatment and management issues trigger NIMBY movements, and disputes among states, governments, and social actors over the issues have been accelrating. At the level of states and sub-states, various measures including institutions, laws, regulations, market-oriented methods, consensus and collaborative governance have been introduced to address various problems associated with waste. More recently, science and technology has been applied to the waste treatment and management under the notion of circular economy. However, societal support for addressing waste problems still remains inadequate, calling for new approaches and alternative pathways. In this context, this study examines two cases of urban incineration plants designed by Hudertwasser, Austria-born artist and architect: Spittelau, Austria, and Osaka, Japan. Through these case studies, I demonstrate how creative and innovative culture-technology convergence can shed new light on challenging environmental issues such as an urban waste problem.

• Horticultural Science & Technology
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• v.33 no.6
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• pp.891-899
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• 2015

The lily symptomless virus (LSV) is the most common virus in Korean native lilies and causes various types of damage to overall plant growth. This study was carried out to investigate the elimination rate of the LSV by the in vitro scale culture (scaling) method in Korean native lilies and to test reinfection rates of the LSV under several field culture conditions of bulb production. Four Korean native lilies (Lilium dauricum, L. distichum, L. lancifolium, and L. maximowitzii) were used and their scales were cultured in vitro for micro-scale formation. The micro-scales were subcultured repeatedly using MS culture medium supplemented with 30 or $90g{\cdot}L^{-1}$ sucrose. The culture conditions were $24{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ PPFD with 16 hour daylength using fluorescent lamps and maintained at $22{\pm}2^{\circ}C$. The virus-free bulblets were grown for one to three years in the greenhouse and transplanted to the field in October or March. Virus infection rates were investigated by direct tissue blotting immunobinding assays and measurement of chlorophyll and protein contents. Virus-free plants could be obtained from the 5th subculture of micro-scales in L. lancifolium and L. maximowitzii or from primary culture in L. dauricum and L. distichum. LSV-free plants were reinfected during bulb production in the field. Reinfection rates were higher at older bulb ages and under higher planting density. The plants planted in October and at inland Gyeongsan had higher infection rates than those planted in March and at coastal area Pohang. The reinfection rate of L. maximowitzii was higher than those of L. dauricum and L. lancifolium. The LSV-infected plants had lower chlorophyll contents and unchanged protein contents compared to virus-free plants.

• Journal of Bio-Environment Control
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• v.23 no.4
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• pp.281-287
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• 2014

This study was carried out to investigate the effect of transplanting methods on the growth and yield of paprika (Capsicumannuum L. 'Veyron' and 'Coletti') in coir culture during two seasons. The summer type sowed in late winter and harvested from summer and the winter type sowed in summer and harvested from early winter. Control plants grown on the 10cm rockwool block were transplanted on coir slab when the 8 leaves of seedlings were emerged, while plants of the young seedling transplanting(YST) grown on the 7cm rockwool block was put on the slab at time of 2-3 leaves developed. Plants of the temporary transplanting(TT) on the 10cm rockwool block were moved on the slab after 2-3 weeks underpinning cultivation, while plants of the blockless transplanting(BT) were directly transplanted in the slab when the seedlings have 2-3 leaves emerged. The plant height of the control and BT treatment were longer while that of TT showed the shortest among treatments. The bigger leaf size was observed in the YST and BT treatment. Leaf number of the BT treatment was increased, while that of TT was the lowest. There were no differences in fruit size, locules and thickness among treatments. The lower fruit weight was observed in the TT of the winter culture and fruits in the control and YST of the summer culture showed higher fruit weight. The percentage of marketable fruit appeared to be slightly higher in the winter culture than in the summer culture. There were no differences in marketable fruit rate among the treatments of the winter culture but, among the summer culture, the highest marketable fruit rate was observed in the BT with 93%. The yield of the YST and BT was higher and that of the TT was the lowest.

• Nuclear Engineering and Technology
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• v.54 no.6
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• pp.2067-2076
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• 2022

The importance of safety culture has been emphasized to achieve a high level of safety. In this light, a systematic method to more properly deal with safety culture is necessary. Here, a decision-making tool that can apply a graded approach to the analysis of safety culture is proposed, called the F-D matrix, which determines the frequency and the difficulty of safety culture attributes recently defined by the IAEA. A hierarchical model of difficulty contributors was developed as a scoring standard, and its elements were weighted via expert evaluation using the analytic hierarchy process. The frequency of the attributes was derived by analyzing reported events from nuclear power plants in the Republic of Korea. Period-by-period comparisons with the F-D matrix can show trends in the change of the maturity level of an organization's safety culture and help to evaluate the effectiveness of previously implemented measures. In the evaluating the difficulty of the attributes in the recently developed harmonized safety culture model, the difficulties of Trending, Benchmarking, Resilience, and Documentation and Procedures were found to be relatively high, while the difficulties of Conflicts are Resolved, Ownership, Collaboration, and Respect is Evident were found to be relatively low. A case study was conducted with an analysis period of 10 years to attempt to reflect the many changes in safety culture that have been made following the Fukushima accident in March 2011. As a result of comparing two periods following the Fukushima accident, the overall frequency decreased by about 40%, providing evidence for the effects of the various improvements and measures taken following the increased emphasis on safety culture. The proposed F-D matrix provides a new analytical perspective and enables an in-depth analysis of safety culture.