• Title/Summary/Keyword: Plants Culture

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In vitro Regeneration and Genetic Stability Analysis of the Regenerated Green Plants in Japanese Blood Grass (Imperata cylindrica 'Rubra') (홍띠 기내 재생과 재생 녹색식물체의 유전적 안정성)

  • Kang, In-Jin;Lee, Ye-Jin;Bae, Chang-Hyu
    • Korean Journal of Plant Resources
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    • v.34 no.2
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    • pp.156-165
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    • 2021
  • The in vitro regeneration was established, and the genetic stability among the mother plants (control) and the micropropagated green plants was evaluated using ISSR markers in Imperata cylindrica 'Rubra', Poaceae which containing important bioenergy plants. Green shoots were multiply induced from growing point culture via callus on MS medium supplemented with 0.01 mg/L NAA and 2 mg/L BA, and the shoots were proliferated on the MS medium with rooting. Rooted plantlets were transplanted to the pot with 100% survival rate. Using ISSR markers, somaclonal variation was analyzed in eight mother plants (control), ten green-regenerant cultivated at culture room (ReR) and ten green-regenerant cultivated at field condition (ReF). All ISSRs produced a total of 97 bands, and the scorable bands varied from one to seven with an average of 4.4 bands per primer. The polymorphism rate of ReRs and ReFs was 4.1% and 3.1% respectively, showing higher rate than that of control (0%). The genetic similarity matrix (GSM) among all accessions ranged from 0.919 to 1.0 with a mean of 0.972. According to the clustering analysis, ReFs and mother plants were divided into two independent groups. The results indicate that no clear genetic diversity was detected among regenerated plants, and ISSR markers were useful tool for identification of somaclonal variation of regenerants.

Effect of a Bacterial Grass Culture on the Plant Growth and Disease Control in Tomato

  • Lee, Yong Seong;Naing, Kyaw Wai;Kim, Kil Yong
    • Research in Plant Disease
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    • v.23 no.4
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    • pp.295-305
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    • 2017
  • This study aimed to investigate the plant growth-promoting and biocontrol potential of a grass culture with Paenibacillus ehimensis KWN8 on tomato. For this experiment, treatments of a chemical fertilizer (F), a bacterial grass culture (G), a 1/3 volume of G plus 2/3 F (GF), and F plus a synthetic fungicide (FSf) were applied to tomato leaves and roots. The result showed that the severity of Alternaria solani and Botrytis cinerea symptoms were significantly reduced after the application of the bacterial grass culture (G and GF) and FSf. In addition, root mortality in G and GF was lower compared to F. Tomato plants treated with G or GF had better vegetative growth and yield compared to F. Application of G affected the fungal and bacterial populations in the soil. In conclusion, treatment with a bacterial grass culture decreased disease severity and increased tomato growth parameters. However, there were no statistically significant correlations between disease occurrence and tomato yields. This experiment presents the possibility to manage diseases of tomato in an environmentally friendly manner and to also increase the yield of tomato by using a grass culture broth containing P. ehimensis KWN38.

Studies on the Culture of Ergot Fungus(IV) -Culture of Korean Ergot Fungus- (맥각균(麥角菌)의 배양(培養)에 관한 연구(硏究)(IV) -한국산(韓國産) 맥각균(麥角菌)의 배양(培養)-)

  • Kim, Byong-Kak;Shim, Mi-Ja;Choi, Eung-Chil;Park, Young-In
    • Korean Journal of Pharmacognosy
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    • v.6 no.1
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    • pp.9-13
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    • 1975
  • To isolate ergot strains which are capable of growing and producing alkaloids in submerged culture, strains were isolated from the sclerotia parasitizing the Graminae plants in Korea and the experiments of submerged culture of these strains yielded the following results: 1) The mycelia which were respectively isolated from the sclerotia parasitizing Agropyron semicostatum $N_{EES}$, Arundinella hirta $T_{ANAKA}$ var. ciliata $K_{OIDSUMI}$, Ischaemum anthephoroides $M_{IQ}$. var. eriostachyum $H_{ONDA}$, and Phleum pretense L. grew in Medium D by submerged culture. 2) When the strain of the ergot of Agropyron semicostatum was inoculated into six different nutrient solutions for submerged culture, its mycelium grew well in Media C, D and F, but produced alkaloids only in Medium C, indicating that Medium C is relatively suitable for the strain. 3) The extraction of the alkaloids from the culture broth by ether and T.L.C. analysis of the extract showed that it contained at least two types of alkaloids.

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New Disese of Wheat and Barley Caused by Fusarium (Calonectria) nivale in Korea (Fusarium(Calonectria) nivale에 의한 맥류의 신병해)

  • Sung J. M.;Chung B. J.;Snyder W. C.
    • Korean journal of applied entomology
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    • v.16 no.4 s.33
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    • pp.209-210
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    • 1977
  • Early in the growing season of 1976, rain and cool weather favored the blighting of leaves of young plants of barley by Fusarium nivale. The fungus was recovered in culture from infected foliage, and the perithecia of Calonectria nivalis were demonstrated to be present as well as the Fusarium state. On 22 April 1976, in Suweon, plants pulled at random revealed stem lesions from which F. nivale was cultured. On S May 1976 near Kwangju. Perithecia were found embedded within leaf sheaths and blades of mature wheat and barley plants. It was evident in the 1976, 1977 season that Fusarium(Calonectria) nivale was common but unrecognized as an early season pathogen of barley in Korea. The probable source of primary inoculum was the infected refuse from the previous season's barlry and wheat crops. Stem lesions caused by this fungus were considered to he detrimental to the maximum yield of barley or wheat.

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In Vitro Propagation of Medicinal Herbs in Korea

  • An, Chanhoon;Song, Jeongho
    • Journal of Forest and Environmental Science
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    • v.34 no.1
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    • pp.77-81
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    • 2018
  • Mass production of forest medicinal plants is related to quality control of raw medicinal materials. Plant tissue culture is an important technology to produce high-quality plant materials. Numerous factors are reported to influence the success of in vitro regeneration of medicinal plants. Embryogenesis is known to be the most effective techniques and it has developed in some medicinal plant species. Various in vitro cultural condition for direct and/or indirect somatic embryogenesis systems have developed in Epimedium koreaum, Bupleurum falcatum, Paeonia lactiflora, Chrysanthemum zawadskii, Houttuynia cordata etc. In this study, we provide the present statue and information of in vitro propagation techniques that is able to apply as an efficient system for rootstock propagation system of forest medicinal plants.

The Use of Natural Dye Fabrics to Design of Works (천연염직물을 이용한 작품디자인 연구)

  • 정진순
    • Journal of the Korean Society of Costume
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    • v.52 no.2
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    • pp.91-101
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    • 2002
  • In order to development of culture goods I made works with various color's silk fabrics dyed with water extract or methanol extract of plants. The Plants used for dyeing as dyeing material were indigo, safflower, amur cock tree, onion's Peel, citrus peel, mugwort, gromwell, sappan wood. Each of Plants were extracted by each of temperature and time. The mordants used for various color chanties are aluminium sulfate, cupric acetate mono hydrate, ferrous chloride. With fabrics of various color dyed like this I made works which give expression to an easy and natural image of the Korean nation of fabrics.

Commercialization of Genetically Engineered Plants in the United States: Overview Examples, and Future Prospects

  • Wilkinson, Jack Q.
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.4
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    • pp.203-212
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    • 1997
  • The impact of plant genetic engineering, a technology born in the early 1980's, is beginning to be felt across the world in the 1990's. The first wave of engineered plant produce are reaching consumers in the supermarket and many more are destined to follow Transformation technology now exists for most plant, including the four staple crops-maize, wheat, rice, and soybean. Early targets of genetic engineering include plane possessing insect resistance and herbicide tolerance, with future goals set on increasing harvestable yield, improving nutritional quality, and making specialty products. This review describes some of the milestones in plant biotechnology, the U.S. regulatory agencies, field trial numbers and deregulated plants, commercialization criteria, examples of commercialized plants, and future prospects of plant biotechnology.

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Fashion illustration by utilizing Four gentlemanly plants motive (사군자의 디자인모티브를 응용한 패션일러스트레이션 연구)

  • 하윤경;김혜경
    • Journal of the Korean Society of Costume
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    • v.51 no.8
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    • pp.159-170
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    • 2001
  • The culture and society environment plays major role in the development of Fashion and now in the 21st century, interest in Fashion is getting increased along with the rapid progress of advanced media development and information surge. Now it s the time to extend visual efficiency not only transmitting fashion image but also develop a creative product that satisfy the demander s needs. Currently it is very hard to express our subjective Korean "nationalism" in the Fashion since the various expression skill using western fashion illustration are dominant in our society. I have classified two components, fashion illustration and Korean painting in the theoretical circumstance. In fashion illustration circumstance, I examined the concept, usage, expression skill and history referring to historical documents. In Korean painting section, I considered coloring portrait concept, pattern, history, concept, and feature of the four gentlemanly plants. including the basic material of Korean-paper, Writing- Brush, Ink-Stone, Traditional-ink and also the history of itself. I have designed 8 products applying the concept of plum blossoms, orchids, chrysanthemums and bamboos that represent the most important part of the four gentlemanly plants, and also the expression skill of Korean coloring portrait.rait.

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Uptake of Soilmicrobial Metabolites and Allelochemicals in Plant Root System (식물의 뿌리에 의한 토양 미생물 대사산물 및 Allelochemicals의 흡수)

  • Kim, M.J.;Lee, Y.S.
    • Korean Journal of Environmental Agriculture
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    • v.16 no.3
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    • pp.264-268
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    • 1997
  • Microbial metabolites from rhizosphere soil samples mainly inhabitated by Streptomyces are selectively uptaken into plants. The culture broth of a Streptomyces strain K9301 showed a major metabolite which disappeared in the medium 24hrs after planting of seedlings. This metabolite was selectively uptaken in the rice plants as well as the wheat plants. We identified the targeted metabolite showing a strong UV-absorbing spot at Rf 0.6 on TLC to be 2-aminobenzamide.

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Programmed Cell death in plants

  • Fukuda, Hiroo
    • Proceedings of the Botanical Society of Korea Conference
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    • 1999.07a
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    • pp.69-73
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    • 1999
  • In plants as well as in other multicellular organisms, programmed cell death plays essential roles in the abortion or formation of specific cells and tissues during development to organize the plant [11, 15, 18]. A typical example of developmentally programmed cell death in plants is the death during differentiation of tracheary elements which are components of vessels and tracheids, a water-conducting system. The programming of cell death during tracheary element differentiation has been revealed to be unique to plant cells by using the in vitro Zinnia mesophyll cell culture system. In particular, new biosynthesis of autolysis-related enzymes such as cysteine proteases and nucleases, their accumulation of the vacuole and the programmed collapse of the vacuole are essential to the death of tracheary elements and differ greatly from the process of the apoptotic cell death in animals.

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