• Title/Summary/Keyword: Plant-scale

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Development of the Large-Scale Oligonucleotide Chip for the Diagnosis of Plant Viruses and its Practical Use

  • Nam, Moon;Kim, Jeong-Seon;Lim, Seungmo;Park, Chung Youl;Kim, Jeong-Gyu;Choi, Hong-Soo;Lim, Hyoun-Sub;Moon, Jae Sun;Lee, Su-Heon
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.51-57
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    • 2014
  • A large-scale oligonucleotide (LSON) chip was developed for the detection of the plant viruses with known genetic information. The LSON chip contains two sets of 3,978 probes for 538 species of targets including plant viruses, satellite RNAs and viroids. A hundred forty thousand probes, consisting of isolate-, species- and genus-specific probes respectively, are designed from 20,000 of independent nucleotide sequence of plant viruses. Based on the economic importance, the amount of genome information, and the number of strains and/or isolates, one to fifty-one probes for each target virus are selected and spotted on the chip. The standard and field samples for the analysis of the LSON chip have been prepared and tested by RT-PCR. The probe's specific and/or nonspecific reaction patterns by LSON chip allow us to diagnose the unidentified viruses. Thus, the LSON chip in this study could be highly useful for the detection of unexpected plant viruses, the monitoring of emerging viruses and the fluctuation of the population of major viruses in each plant.

Current Status and Prospect of Environmental friendly Farmstead Milk Processing in Korea (한국의 친환경적 목장형 유가공의 현황과 발전과제)

  • Bae, In-Hyu
    • Korean Journal of Organic Agriculture
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    • v.18 no.2
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    • pp.155-176
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    • 2010
  • This study was conducted to research the status, history and prospects of farm scale milk processing and to develop a management strategy for small scale milk process plant in Korea. Also it aims to provide ways to apply it so as to vitalize the farm made milk products market practically. This study was also treats the practical development of dairy farm school programs through the farm scale milk processing. Farm-scale milk plant (FMP) should be some of the ideas to develop small scale and using the resources according to the local features, limited expanding in regional market, produce by consumers order amounts, management policy will be transferred organic dairy farm. A few policy suggestions to put FMP system of financial support would not from beginner, it is better to settled FMP system by government or co-operation group in practical support programs were proposed. What the state needs to do through direct involvement were to put efforts at demand expansion on FMP system products, to certificate and safety the farm made milk products marketing system settings, to build more variation chance of the milk products. What was more important, however, was support policy, to create the network of FMP market and to develop of training program contents for each FMP operation unit. The ideal FMP model for the development of Dairy Farming proposed in this research will be applied as a relevant reference in managing and realizing environmental friendly and sustainable dairy industry at the national level.

Optimization of Supercritical Water Oxidation(SCWO) Process for Decomposing Nitromethane (Nitromethane 분해를 위한 초임계수 산화(SCWO) 공정 최적화)

  • Han, Joo Hee;Jeong, Chang Mo;Do, Seung Hoe;Han, Kee Do;Sin, Yeong Ho
    • Korean Chemical Engineering Research
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    • v.44 no.6
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    • pp.659-668
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    • 2006
  • The optimization of supercritical water oxidation (SCWO) process for decomposing nitromethane was studied by means of a design of experiments. The optimum operating region for the SCWO process to minimize COD and T-N of treated water was obtained in a lab scale unit. The authors had compared the results from a SCWO pilot plant with those from a lab scale system to explore the problems of scale-up of SCWO process. The COD and T-N in treated waters were selected as key process output variables (KPOV) for optimization, and the reaction temperature (Temp) and the mole ratio of nitromethane to ammonium hydroxide (NAR) were selected as key process input variables (KPIV) through the preliminary tests. The central composite design as a statistical design of experiments was applied to the optimization, and the experimental results were analyzed by means of the response surface method. From the main effects analysis, it was declared that COD of treated water steeply decreased with increasing Temp but slightly decreased with an increase in NAR, and T-N decreased with increasing both Temp and NAR. At lower Temp as $420{\sim}430^{\circ}C$, the T-N steeply decreased with an increase in NAR, however its variation was negligible at higher Temp above $450^{\circ}C$. The regression equations for COD and T-N were obtained as quadratic models with coded Temp and NAR, and they were confirmed with coefficient of determination ($r^2$) and normality of standardized residuals. The optimum operating region was defined as Temp $450-460^{\circ}C$ and NAR 1.03-1.08 by the intersection area of COD < 2 mg/L and T-N < 40 mg/L with regression equations and considering corrosion prevention. To confirm the optimization results and investigate the scale-up problems of SCWO process, the nitromethane was decomposed in a pilot plant. The experimental results from a SCWO pilot plant were compared with regression equations of COD and T-N, respectively. The results of COD and T-N from a pilot plant could be predicted well with regression equations which were derived in a lab scale SCWO system, although the errors of pilot plant data were larger than lab ones. The predictabilities were confirmed by the parity plots and the normality analyses of standardized residuals.

Modeling of a Small Group Scale TMR Plant for Beef Cattle and Dairy Farm in Korea(II) - Performance Test and Cost Analysis of the Model Plant - (한우 및 낙농 단지용 소형 TMR 플랜트 모델 개발(II) - 모델의 성능시험 및 경제성분석 -)

  • Ha, Yu-Shin;Hong, Dong-Hyuck;Park, Kyung-Kyoo
    • Journal of Biosystems Engineering
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    • v.35 no.2
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    • pp.91-99
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    • 2010
  • A Model of small scale total mixed rations(TMR) plant which can be utilized round bales was developed, tested and analyzed in this study. This study consist of two parts. One is development of a small scale TMR plant model which was already reported at the previous paper. This is the second part of the study. For the study, a series of tests of the model plant were performed and its costs was analyzed. Also, the break-even point of the model plant by comparing with market price of commercial TMR feed was determined. Results of the research are summarized as follows ; As the results of mixing test, the average coefficient of variation(CV) value for mixing of the feed was 13.0 % at the gate of the mixer. The production cost was estimated as 8,298 won/head for dairy cattle farm and 2,495 won/head for beef cattle farm, when producing 8 batch a day. Also, it is recommended to utilize the model plant when farm size is over 79 heads for dairy cattle farm and 113 heads for beef cattle farm. As an overall conclusion, the model plant designed for farm size TMR feed mill will be very useful model for both beef cattle and dairy farms in Korea. Also it is expected that the capital investment for the model plant can be recovered with 8 months compare with purchasing commercial TMR feed if the model plant feeds 1,000 beef cattle approximately.

Development of superconducting high gradient magnetic separation system for scale removal from feed-water in thermal power plant

  • Shibatani, Saori;Nakanishi, Motohiro;Mizuno, Nobumi;Mishima, Fumihito;Akiyama, Yoko;Okada, Hidehiko;Hirota, Noriyuki;Matsuura, Hideki;Maeda, Tatsumi;Shigemoto, Naoya;Nishijima, Shigehiro
    • Progress in Superconductivity and Cryogenics
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    • v.18 no.1
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    • pp.19-22
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    • 2016
  • A Superconducting High Gradient Magnetic Separation (HGMS) system is proposed for treatment of feed-water in thermal power plant [1]. This is a method to remove the iron scale from feed-water utilizing magnetic force. One of the issues for practical use of HGMS system is to extend continuous operation period. In this study, we designed the magnetic filters by particle trajectory simulation and HGMS experiments in order to solve this problem. As a result, the quantity of magnetite captured by each filter was equalized and filter blockage was prevented. A design method of the magnetic filter was proposed which is suitable for the long-term continuous scale removal in the feed-water system of the thermal power plant.

Plant cell culture strategies for the production of natural products

  • Ochoa-Villarreal, Marisol;Howat, Susan;Hong, SunMi;Jang, Mi Ok;Jin, Young-Woo;Lee, Eun-Kyong;Loake, Gary J.
    • BMB Reports
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    • v.49 no.3
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    • pp.149-158
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    • 2016
  • Plants have evolved a vast chemical cornucopia to support their sessile lifestyles. Man has exploited this natural resource since Neolithic times and currently plant-derived chemicals are exploited for a myriad of applications. However, plant sources of most high-value natural products (NPs) are not domesticated and therefore their production cannot be undertaken on an agricultural scale. Further, these plant species are often slow growing, their populations limiting, the concentration of the target molecule highly variable and routinely present at extremely low concentrations. Plant cell and organ culture constitutes a sustainable, controllable and environmentally friendly tool for the industrial production of plant NPs. Further, advances in cell line selection, biotransformation, product secretion, cell permeabilisation, extraction and scale-up, among others, are driving increases in plant NP yields. However, there remain significant obstacles to the commercial synthesis of high-value chemicals from these sources. The relatively recent isolation, culturing and characterisation of cambial meristematic cells (CMCs), provides an emerging platform to circumvent many of these potential difficulties.

A new record of Carex foraminata (Cyperaceae) in Korean flora

  • Jung-Hyun KIM;Jin-Seok KIM;Chang Woo HYUN;Bongsu CHOI
    • Korean Journal of Plant Taxonomy
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    • v.52 no.4
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    • pp.246-250
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    • 2022
  • We report a new distribution of Carex foraminata C. B. Clarke on the Korean Peninsula. This species was first reported in China and is distributed in Jiangxi and Zhejiang Provinces. We found this species on Hongdo Island in Heuksan-myeon, Shinan-gun, Jeollanam-do in Korea. Carex foraminata is similar to the related taxa C. brevispicula G. H. Nam & G. Y. Chung, C. chungii Z. P. Wang, and C. genkaiensis Ohwi in that its achenes are constricted in the middle part. However, C. foraminata is distinguished from C. brevispicula and C. chungii by its pistillate scale apexes, which are acute or shortly awned and by its achene apexes, which are shortly cylindrical; C. foraminata is distinguished from C. genkaiensis by its pistillate scale and achene shape. A precise description, illustration, photographs, and a key to related taxa are provided.