• Title/Summary/Keyword: Plant-scale

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Bioprocess Considerations for Production of Secondary Metabolites by Plant Cell Suspension Cultures

  • Chattopadhyay, Saurabh;Farkya, Sunita;Srivastava, Ashok K.;Bisaria, Virendra
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.7 no.3
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    • pp.138-149
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    • 2002
  • Plant cell culture provides a viable alternative over whole plant cultivation for the production of secondary metabolites. In order to successfully cultivate the plant cells at large scale, several engineering parameters such as, cell aggregation, mixing, aeration, and shear sensitivity are taken into account for selection of a suitable bioreactor. The media ingredients, their concentrations and the environmental factors are optimized for maximal synthesis of a desired metabolite. Increased productivity in a bioreactor can be achieved by selection of a proper cultivation strategy (batch, fed-batch, two-stage etc.), feeding of metabolic precursors and extraction of intracellular metabolites. Proper understanding and rigorous analysis of these parameters would pave the way towards the successful commercialization of plant cell bioprocesses.

An Study on Priority Determining for Development Strategies of Plant Factory Using Analytic Hierarchy Process and Likert Scale (식물공장 발전방향 설정을 위한 정책 우선순위 도출)

  • Kim, Yean-Jung;Han, Hye-Sung;Kim, Bae-Sung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.11
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    • pp.5570-5575
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    • 2013
  • This study was carried out to find prioritization and evaluation of importance factor for plant factory development direction. According to the results, experts have put profitability secure at the top of development of plant factory industry. It is urgently needed in economic sector that products from plants factory secure the market and adjust the shipping date through contract cultivation and year-round production. Also, illuminant technology is also important in technology development section and R&D. On the other hand, consumers do not yet fully understand the benefits of the plant factory, and this will lead to continued slow sales. Therefore, the government needs to draw up measures to support producers who cultivated agricultural products from plant factory.

Estimate on Economical Optimum Scale of Public Livestock Manure Treatment Plant (가축분뇨 공공처리시설의 경제적 적정규모 설정)

  • Kim, J.H.;Park, C.H.;Kwag, J.H.;Choi, D.Y.;Jeong, K.H.;Chung, U.S.;Chung, Y.B.;Yoo, Y.H.
    • Journal of Animal Environmental Science
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    • v.14 no.1
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    • pp.23-30
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    • 2008
  • The objective of this study was to estimate the optimum scale of PLMTP (Public Livestock Manure Treatment Plant) for the efficient management of public sector by long-run cost function. An economic analysis was performed using the survey of 52 PLMTP records collected by Ministry of Environment in 2007. The main results obtained in this study can be summed up as follows. The optimum scale under given environmental conditions turned out to be $180{\sim}200m^3$/day which is almost $1.5{\sim}1.6$ times of the average scale of sample plants, $146m^3$/day. This gap between the optimum and current scale suggests that there remains the possibility of further expansion of scale.

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A Study on the Effect of Containment Filtered Venting System to Off-site under Severe Accident (중대사고시 격납건물여과배기계통(CFVS)적용으로 인한 사고영향과 결과 고찰)

  • Jeon, Ju Young;Kwon, Tae-Eun;Lee, Jai-Ki
    • Journal of Radiation Protection and Research
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    • v.40 no.4
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    • pp.244-251
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    • 2015
  • The containment filtered venting system reduces the range of the contamination area around the nuclear power plant by strengthening the integrity of the containment building. In this study, the probabilistic assessment code MACCS2 was used to assess the effect of the CFVS to off-site. The accident source term was selected from a Probabilistic Safety Analysis report of SHINKORI 1&2 Nuclear Power Plant. The three source term categories from 19 STC were chosen to evaluate the effective dose and thyroid dose of residents around the power plant and the dose with CFVS and without CFVS were compared. The dose was calculated according to the distance from the nuclear power plant, so the damage scale based on the distance that exceeds the IAEA criteria for effective dose (100 mSv per 7 days) and thyroid dose (50 mSv per 7 days) were compared. The effective dose reduction rates of the STC-3, STC-4, STC-6 were about 95-99% in the whole range (0~35 km), 96-98% for the thyroid dose. There are similar results between effective dose and thyroid dose. After applying the CFVS, the damage scale that exceeds the effective dose criteria was about 1 km (mean). Especially, the STC-4 damage scale was decreased from 26 km (mean) to 1.2 km (mean) significantly. The damage scale that exceed the thyroid dose criteria was decreased to 2~3 km (mean). The STC-4 damage scale was also decreased significantly as compared to STC-3, STC-6 in terms of effective dose.

Large-scale Virtual Power Plant Management Method Considering Variable and Sensitive Loads (가변 및 민감성 부하를 고려한 대단위 가상 발전소 운영 방법)

  • Park, Yong Kuk;Lee, Min Goo;Jung, Kyung Kwon;Lee, Yong-Gu
    • Journal of the Institute of Electronics and Information Engineers
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    • v.52 no.5
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    • pp.225-234
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    • 2015
  • Nowadays a Virtual Power Plant (VPP) represents an aggregation of distributed energy resource such as Distributed Generation (DG), Combined Heat and Power generation (CHP), Energy Storage Systems (ESS) and load in order to operate as a single power plant by using Information and Communication Technologies, ICT. The VPP has been developed and verified based on a single virtual plant platform which is connected with a number of various distributed energy resources. As the VPP's distributed energy resources increase, so does the number of data from distributed energy. Moreover, it is obviously inefficient in the aspects of technique and cost that a virtual plant platform operates in a centralized manner over widespread region. In this paper the concept of the large-scale VPP which can reduce a error probability of system's load and increase the robustness of data exchange among distributed energy resources will be proposed. In addition, it can directly control and supervise energy resource by making small size's virtual platform which can make a optimal resource scheduling to consider of variable and sensitive load in the large-scale VPP. It makes certain the result is verified by simulation.

Adventitious root induction in Ophiorrhiza prostrata: a tool for the production of camptothecin (an anticancer drug) and rapid propagation

  • Martin, Kottackal Poulose;Zhang, Chun-Lai;Hembrom, Manoj Emanuel;Slater, Adrian;Madassery, Joseph
    • Plant Biotechnology Reports
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    • v.2 no.2
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    • pp.163-169
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    • 2008
  • Roots of Ophiorrhiza prostrata D. Don serve as a rich source of camptothecin (CPT), an anticancer drug. Because of the large-scale collection of its roots, the plant has become a threatened species. The present study accomplishes the induction of adventitious roots as a means for the production of CPT as well as for the large-scale propagation of this anticancer drug plant using leaf and internode explants. The biomass yield and CPT content of adventitious roots induced from different explants were compared to roots developed on ex vitro rooted stem cuttings. Adventitious roots were produced on half-strength Murashige and Skoog (MS) medium supplemented with $10.74{\mu}M$ ${\alpha}-naphthaleneacetic$ acid and $2.32{\mu}M$ kinetin at mean fresh weights of 0.753, 0.739 and 0.748 g roots from leaf, internode and shoot, respectively. CPT yield from in vitro derived roots after 50, 80 and 120 days of incubation (0.028, 0.06 and 0.1% dry weight, respectively) was not significantly different from those harvested at the same age from ex vitro rooted (0.03, 0.06 and 0.13%, respectively) stem cuttings. CPT from subcultured roots derived from solid (0.08%) medium was lower than from suspension culture medium (0.12%). Subsequent cultures of the adventitious roots showed a stable production of CPT (0.16%). The yield of CPT from 360-day-old plant-derived roots was 0.19%. Elicitation using methyl jasmonate and acetyl salicylic acid exhibited no enhancement in CPT yield. In vitro propagation through direct shoot regeneration was achieved from the adventitious roots upon transfer to MS medium with $8.87{\mu}M$ $N^6-benzyladenine$ (BA) and $2.46{\mu}M$ indole-3-butyric acid (IBA) with a mean of 21.2 shoots per culture in 50 days. The shoots upon subculture on medium having the same level of BA and IBA underwent rapid proliferation. The shoots transferred to field conditions after in vitro rooting exhibited 95% survival. Adventitious root induction, from leaf and internode explants, enables the feasible production of CPT as well as the large-scale rapid propagation of this species which can safeguard it from extinction.

Treatment of Spent ion-Exchange Resins from NPP by Supercritical Water Oxidation(SCWO) Process (초임계수 산화공정에 의한 원전 폐수지 처리기술)

  • Kim, Kyeong-Sook;Son, Soon-Hwan;Song, Kyu-Min;Han, Joo-Hee;Han, Kee-Do;Do, Seung-Hoe
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.7 no.3
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    • pp.175-182
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    • 2009
  • The spent cationic exchange resins and anionic exchange resins were separated from mixed spent exchange resins by a fluidized bed gravimetric separator. The separated resins were identified by an elemental analysis and thermogravimetric analysis. The each test sample was prepared by diluting the slurry made by wet ball milling the cationic exchange resins and the anionic exchange resins separated as a spherical granular form for 24 hours. The resulting test samples showed a slurry form of less than $75{\mu}m$ of particle size and 25,000ppm of $COD_{cr}$. The decomposition conditions of each test samples from a thermal power plant were obtained with a lab-scale(reactor volume : 220mL) supercritical water oxidation(SCWO) facility. Then pilot plant(reactor volume : 24 L) tests were performed with the test samples from a thermal power plant and a nuclear power plant successively. Based on the optimal decomposition conditions and the operation experiences by lab-scale facility and the pilot plant, a commercial plant(capacity : 150kg/h) can be installed in a nuclear power plant was designed.

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Optimum Pre-treatment Method in Constructed Wetlands by Natural Purification Method for Treating Livestock Wastewater (자연정화공법에 의한 인공습지에서 효과적인 축산폐수처리를 위한 최적 전처리방법 구명)

  • Park, Jong-Hwan;Seo, Dong-Cheol;Kim, Ah-Reum;Kim, Sung-Hun;Lee, Seong-Tea;Jeong, Tae-Uk;Choi, Jeong-Ho;Lee, Sang-Won;Cho, Ju-Sik;Kim, Hyun-Ook;Heo, Jong-Soo
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.425-433
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    • 2011
  • In order to obtain optimum pre-treatment methods and improve T-N and T-P removal efficiencies, removal rates of pollutants in small-scale livestock wastewater treatment apparatus with water plant filtration bed or activated sludge tank were investigated. Based on the results from the optimum pre-treatment in small-scale livestock wastewater treatment apparatus, removal efficiencies of pollutants in livestock wastewater treatment plant with water plant filtration and activated sludge beds. The removal rates of COD, SS, T-N, and T-P in effluent were 83, 89, 63 and 87% in small-scale livestock wastewater treatment apparatus with water plant filtration bed, respectively. The removal rates of COD, SS, T-N, and T-P in effluent were 96, 95, 86 and 92% in small-scale livestock wastewater treatment apparatus with activated sludge tank, respectively. For increasing the COD, SS, T-N, and T-P removals in small-scale livestock wastewater treatment apparatus, the water plant filtration and activated sludge beds are recommended. In livestock wastewater treatment plant with water plant filtration ($1^{st}$ treatment) and activated sludge ($2^{nd}$ treatment) beds, the concentrations of COD, SS, T-N, and T-P in effluent were 39, 15, 42 and $1mg\;L^{-1}$, respectively. It was shown that the concentrations of COD, SS, T-N, and T-P met acceptable effluent quality standard for livestock wastewater. Based on the above results, the removal rates of COD, SS, T-N, and T-P in effluent were over 99.8, 99.9, 99.2, and 99.9% in livestock wastewater treatment plant, respectively.

Efficient Plantlet Regeneration via Callus Formation from Leaf Segment of Lilium Oriental Hybrid 'Casa Blanca'

  • Kim Mi-Sun;Jeon Jae-Heung;Youm Jung-Won;Kim Jae-Hyun;Lee Byung-Chan;Kang Won-Jin;Kim Hyun-Soon;Joung Hyouk
    • Journal of Plant Biotechnology
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    • v.7 no.2
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    • pp.129-134
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    • 2005
  • Callus induction from a leaf explant has been achieved in Lilium Oriental hybrid 'Casa Blanca'. The highest frequency of callus induction was obtained on MS medium supplemented with 0.5 mg/L BA and 2.0 mg/L NAA after 2 months of culture. The cultures maintained continuously without change in color and type of callus when they cultured in the dark. Plantlet regeneration with a high frequency was achieved from induced calli on the same medium. A number of shoots are formed from one cluster of callus, and bulblets developed into intact plantlets after transfer to hormone-free MS medium. No phenotypic variations were observed among regenerants. Enhancement in plantlet regeneration via callus formation would be expected to facilitate the efficiency of transformation of this Oriental hybrid 'Casa Blanca'.

The Research on Activation Plan for Seawater Desalination Plant Application in Korea (국내 해수담수화 플랜트 적용 활성화 방안 연구)

  • Sohn, Jinsik;Yang, Jeong-Seok;Park, Jinseo
    • Journal of Korean Society of Water and Wastewater
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    • v.23 no.2
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    • pp.251-255
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    • 2009
  • Foreign and domestic seawater desalination plant market investigation was performed to analyze the worldwide trend of seawater desalination plant market and to establish the activation plan for seawater desalination plant application. Water demand and seawater desalination related laws and regulations were investigated and analyzed for the activation plan. RO type and large scale plants are popular nowadays however there are only small plants in island region in Korea. There will be about $1 million\;m^3/day$ deficit in 2015 according to the water demand forecasting from Ministry of Environment and Ministry of Land, Transportation, and Maritime Affairs in Korea. Therefore, it is necessary to activate the domestic application of seawater desalination plant to secure stable water resources. To activate the domestic application of seawater desalination plant, first, we need to establish regulations, support system in the water service law for seawater desalination plant. Second, related Ministry should increase the support for the operation and management of seawater desalination plant and suggest the construction of seawater desalination plant for water resources security near seaside region.