• 제목/요약/키워드: Plant propagation

검색결과 798건 처리시간 0.032초

Influence of basal medium formulations and silver nanoparticles on in vitro plant growth in gerbera

  • Hyun Hee Kang;Aung Htay Naing;Junping Xu;Mi Young Chung;Su Young Lee;Jeung-Sul Han;Chang Kil Kim
    • Journal of Plant Biotechnology
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    • 제50권
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    • pp.183-189
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    • 2023
  • This study investigated the impact of two distinct MS basal media: one containing FeNaEDTA and the other FeEDDHA, on the growth of five unique gerbera cultivars (Shy Pink, Pink Holic, Breeze, Harmony, Snow Dream). Notably, the response to these media types varied significantly among the cultivars, particularly concerning leaf yellowing and plant growth. 'Shiny Pink' and 'Pink Holic' exhibited leaf yellowing on the FeNaEDTA-containing medium but displayed leaf greening on the FeEDDHA-containing medium. In contrast, 'Snow Dream,' 'Harmony,' and 'Breeze' remained unaffected on both medium types. However, the FeNaEDTA-containing medium promoted higher plant height and petiole length in 'Breeze,' 'Harmony,' and 'Snow Dream' than the FeNaEDTA-containing medium did. A promotive effect of silver nanoparticles (AgNPs) on plant growth and leaf greening was observed in 'Pink Holic,' particularly on the FeNaEDTA-containing medium, while the addition of AgNPs to the FeEDDHA-containing medium negatively affected plant growth. These results highlight the substantial influence of medium type, specifically the presence of FeNaEDTA or FeEDDHA, on gerbera growth responses, emphasizing the critical role of medium selection in gerbera propagation. Additionally, when contemplating the addition of AgNPs for in vitro gerbera propagation, it is crucial to consider the medium type.

FDS를 이용한 구획실 백드래프트 현상의 수치적 재현성에 관한 연구 (A Study of Numerical Reproducibility for the Backdraft Phenomena in a Compartment using the FDS)

  • 박지웅;오창보;최병일;한용식
    • 한국안전학회지
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    • 제28권6호
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    • pp.6-10
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    • 2013
  • A numerical reproducibility of the backdraft phenomena in a compartment was investigated. The prediction performance of two combustion models, the mixture fraction and finite chemistry models, were tested for the backdraft phenomena using the FDS code developed by the NIST. The mixture fraction model could not predict the flame propagation in a fuel-air mixture as well as the backdraft phenomena. However, the finite chemistry model predicted the flame propagation in the mixture inside a tube reasonably. In addition, the finite chemistry model predicted well the backdraft phenomena in a compartment qualitatively. The flame propagation inside the compartment, fuel and oxygen distribution and explosive fire ball behavior were well simulated with the finite chemistry model. It showed that the FDS adopted with the finite chemistry model can be an effective simulation tool for the investigation of backdraft in a compartment.

In vitro Propagation of Junos Orange (Citrus junos Sieb.) through Nucellar Polyembroid Cultures

  • Park, Woo-Jin;Kang, Young-Min;Min, Ji-Yun;Park, Dong-Jin;Kim, Yong-Duck;Karigar, C.S.;Choi, Myung-Suk
    • 한국약용작물학회지
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    • 제12권5호
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    • pp.384-390
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    • 2004
  • An in vtro nucellar polyembryo propagation method was established with mature seed of the Citrus junos Sieb. 7-8 nucellar polyembryos per seed were induced on MS basal medium without plant growth regulators. The polyembryos developed to complete plantlets on teatment with IBA. These shoots grew further in MS medium without plant growth regulators. Rooting of shoots occurred on MS medium supplemented with IBA. These plantlets were successfully transplanted to small plastic pot containing soil mixture. Somatic embryos were induced from nucellar polyembryo and maturation occurred spontaneously from proliferating cultures on MS medium without growth regulators. Random Amplified Polymorphic DNA (RAPD) marker analysis of in vitro and in vivo grown junos orange showed identical polymorphism indicative of their genetic stability. The RAPD polymorphism produced revealed same banding pattern in each regenerant. Hence, propagaton of junos orange by nucellalr polyembryos was efficient and produced in genetically stable plants under in vitro conditions.

Micro-propagation Factors Essential for Mass Production of Synthetic Seeds in Banana

  • Hassanein A. M.;Ibrahiem I. A.;Galal A. A.;Salem J. M. M.
    • Journal of Plant Biotechnology
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    • 제7권3호
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    • pp.175-181
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    • 2005
  • This work described some essential factors necessary for micro-propagation of banana for mass production of synthetic seeds for germ plasm conservation, and how peroxides activity of conserved tissue was influenced. Shoot tips of field grown plants were used to obtain shoot clusters on shoot proliferation medium (MS medium supplemented with 5 mg/l BAP). Using longitudinally-split shoot tip technique, 18720, 8640, 7488, 2016 plantlets were obtained from one shoot tip of Maghraby, Grand Naine, Balady, and Williams, respectively, in six subculture, one month each, on solid medium. Shoot tips excised from in vitro grown plantlets were encapsulated in calcium-alginate beads and stored at $4^{\circ}C$ for one month on half-strength MS basal medium without growth regulators or sugars. After one month all the viable-conserved synseeds formed shoots when they were transferred to MS basal medium, some of them showed synchronous formation of shoot and root systems in one week. Plants retrieved from encapsulated shoot tips were hardened off and transferred to soil.

초기 다중고장 실시간 진단기법 개발 및 고리원전 적용 (Real-Time Diagnosis of Incipient Multiple Faults with Application for Kori Nuclear Power Plant)

  • Chung, Hak-Yeong;Zeungnam Bien
    • Nuclear Engineering and Technology
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    • 제27권5호
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    • pp.670-686
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    • 1995
  • 본 논문의 저자는 원자력 발전소와 같은 복잡한 대규모의 시스템의 실시간 고장진단 방법을 1994년 IEEE TNS Vol. 41, No. 4 호[1]에 발표하였다. 이번 논문에서는 고장전파모델(FPM)로서 같은 'Timed SDG Model' 를 사용하고 있으나 고장전파시간( FPT)을 에메논리 개념을 이용하여 정확하게 구하기 어려운 FPT을 실질적으로 이용할 수 있도록 했으며, 또한 고장전파확율(FPP)개념을 도입하여 하나이상의 고장원인 절점 (Node)들을 절점고장율과 더불어, 보다 효과적으로 판별할 수 있도록 했다. 또 FPM내에서 고장의 전파확율를 고려함으로서 보다 실질적인 고장 진단방법을 제시하였으며 본 제안된 방법을 고리 원전 2호기 1차계통에 적용하여 1차계통 FPM내의 각 FPP이 ‘1’인 경우에 한하여 그 성능을 입증하여 보았다.

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팔레높시스의 PLB 증식에 미치는 배지와 배양온도 및 광도의 영향 (Effect of Culture Medium, Temperature, and Light Intensity on PLB Propagation of Phalaenopsis)

  • 김미선;은종선;김재영
    • 식물조직배양학회지
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    • 제28권4호
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    • pp.215-219
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    • 2001
  • 팔레높시스 대량생산시 PLB 증식에 미치는 배지와 배양환경에 대해 조사하였다. PLB 증식량은 MS배지에서 높았지만, 증식된 PLB상태는 NDM배지가 더욱 양호하였다 천연첨가 물인 CW 10% 또는 사과와 감자의 사용은 PLB 증식효율을 향상시키는 데 필수적이었다. 배지내 지지물에 따른 PLB 증신률은 gelrite가 첨가된 고체배지가 흰색과 분홍색계통 모두 액체탈지면 배지보다 증식이 양호하였다. PLB 증식시 적정 sucrose 농도는 10g/L이며 농도가 높아질수록 PLB가 유식물체로 재분화되었고 저농도 일수록 증식과 유식물체의 재분화가 모두 억제되었다. PLB증식을 위한 적정 PPF와 온도는 각각 14.3 $\mu$mol.s$^{-1}$m$^{-2}$ , $25^{\circ}C$이었다.

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세포공학을 이용한 식물개량 (Improvement of Plants by Biotechnology)

  • 윤의수
    • 한국자원식물학회지
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    • 제3권1호
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    • pp.1-30
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    • 1990
  • The traditional plant imprownent methods consisted of pure line selection, cross breeding, heterosis breeding, polyploid breeding, mutati-onbreeding, ect.Biotechmoiogy is divided into gene spliclng , monocle-nal antibodies , protein engineering , agricultural research, and microbiological engineering. Of these , high plants deal with agricultural research, and the importent part of which is tissue culture and celLculture , Tissue .culture and cell culture are again divided into embryoculture, test tube fertilization, anther and pollen culture, somatichybridization , transformation, recombination, recombinant DNA moleculehybrid plasmid, ect For these haploid production, protoplast culture,protoplast fusion, selection and propagation, ect. , the technical sett-lement is needed.

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신경회로망을 이용한 비선형 플랜트의 적응제어 (Adaptive controls for non-linear plant using neural network)

  • 정대원
    • 제어로봇시스템학회:학술대회논문집
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    • 제어로봇시스템학회 1997년도 한국자동제어학술회의논문집; 한국전력공사 서울연수원; 17-18 Oct. 1997
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    • pp.215-218
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    • 1997
  • A dynamic back-propagation neural network is addressed for adaptive neural control system to approximate non-linear control system rather than static networks. It has the capability to represent the approximation of nonlinear system without mathematical analysis and to carry out the on-line learning algorithm for real time application. The simulated results show fast tracking capability and adaptive response by using dynamic back-propagation neurons.

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Clonal Propagation through Leaf Sheath Culture of Phalaenopsis

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Han, Gyu-Heung;Woo, In-Shik
    • Plant Resources
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    • 제5권3호
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    • pp.176-180
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    • 2002
  • This study was conducted to develop the clonal propagation technique through in vitro culture using by leaf sheath explants of Phalaenopsis grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when explants of leaf sheath were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 mVL coconut water, 1 mg/L NAA, 1 mg/L 2ip and 2.5 g/L gelrite. The PLB formation rate of VW medium was highest followed by modified Hyponex medium, and lowest in MS medium. Plantlets induced from PLBs transferred to plastic pots including spagnum moss were well developed.

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