• Title/Summary/Keyword: Plant life

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Molecular Cloning, Characterization and Expression of a Novel Trehalose-6-phosphate Synthase Homologue from Ginkgo biloba

  • Wu, Weisheng;Pang, Yongzhen;Shen, Guo-An;Lu, Jie;Lin, Juan;Wang, Jin;Sun, Xiaofen;Tang, Kexuan
    • BMB Reports
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    • 제39권2호
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    • pp.158-166
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    • 2006

  • In many organisms, trehalose acts as protective metabolite against harsh environmental stresses, such as freezing, drought, nutrient starvation, heat and salt. Herein a cDNA (designated as GbTPS, GenBank Accession Number AY884150) encoding a trehalose-6-phosphate synthase homologue was isolated and characterized from the living fossil plant, Ginkgo biloba, which is highly tolerant to drought and cold. GbTPS encoded an 868-amino-acid polypeptide with a predicted isoelectric point of 5.83 and molecular mass of 97.9 kD. Amino acid sequence alignment revealed that GbTPS shared high identity with class II trehalose-6-phosphate synthase homologues (67% identical to AtTPS7), but had only 17% and 23% of identity with OstA from Escherichia coli and ScTPS1 from S. cerevisiae, respectively. DNA gel blot analysis indicated that GbTPS belonged to a small multi-gene family. The expression analysis by RT-PCR showed that GbTPS expressed in a tissue-specific manner in G biloba and might involve in leaf development. GbTPS was also found to be induced by a variety of stresses including cold, salt, drought and mannitol.

  • https://doi.org/10.5483/BMBRep.2006.39.2.158 인용 PDF

Analysis of Intragenic Ds Transpositions and Excision Events Generating Novel Allelic Variation in Rice

  • Park, Soon Ju;Piao, Hai Long;Xuan, Yuan-hu;Park, Sung Han;Je, Byoung Il;Kim, Chul Min;Lee, Eun Jin;Park, Soo Hyun;Ryu, ByeongChan;Lee, Kon Ho;Lee, Gi Hwan;Nam, Min Hee;Yeo, Un Sang;Lee, Myung Chul;Yun, Doh Won;Eun, Moo Young;Han, Chang-deok
    • Molecules and Cells
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    • 제21권2호
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    • pp.284-293
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    • 2006

  • Even though Ac/Ds gene-tagging systems have been established in many higher plants, maize is the only major plant in which short-distance transposition of Ac/Ds has been utilized to probe gene function. This study was performed to evaluate the efficiency of obtaining new alleles and functional revertants from Ds insertion loci in rice. By analyzing 1,580 plants and the progeny of selected lines, the insertion sites and orientations of Ds elements within 16 new heritable alleles of three rice loci were identified and characterized. Intragenic transposition was detected in both directions from the original insertion sites. The closest interval was 35 bp. Three of the alleles had two Ds elements in cis configuration in the same transcription units. We also analyzed the excision footprints of intragenic and extragenic transpositions in Ds-inserted alleles at 5 loci. The 134 footprints obtained from different plants revealed predominant patterns. Ds excision at each locus left a predominant footprint at frequencies of 30-75%. Overall, 66% of the footprints were 7-bp additions. In addition, 16% of the excisions left 0-, 3-, 6-, and 9-bp additions with the potential of conserving reading frame.

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