• Journal of Plant Biology
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• v.33 no.1
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• pp.73-80
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• 1990

Subcellular distribution of arginase activity was measured in leaves of Canavalia lineata. Both mitochondrial and cytosolic fraction were found to contain the arginase activity. It was noticible that cytosolic fraction contained a substantial amount of arginase activity. Different mobility of arginase from these two fractions was showed on DEAE-Sephacel chromatography and polyacrylamide gel electrophoresis. Also different pI value was showed 6.3 in cytosolic and 6.7, 7.1 in mitochondiral fraction on IEF gel electrophoresis. However, canavaine-dependent-activity (CDA) of arginase in these two fractions were not different. These results indicate that heterogenity of arginase occurs in leaves of C. lineata.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.32 no.3
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• pp.294-301
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• 1987

This experiment was carried out to investigate the occurrence of tiller and its proportion to grain yield under 5 different plant densities. Plant density 5 x 5cm was more favorable for increasing the number of spikes per unit area, where leads to improve grain yield. Effective tillers showed only main stem, M$_1$-axil and M$_2$-axil, indication more effective tillers in M$_1$ rather than main stem. Every tillers held at least 6 leaves to bear spikes, indicating that main stem has 12 -13 leaves. Leaves attached on main stem M$\sub$0/ and M$_1$ were very important as main source of photosynthesis, especially under more compart plant densities while leaves of M$_1$ occupied 66.8% in a plant. The proportion of grain yield of each tiller to total grain yield per plant was high in main stem under conpact plant density, in M$_1$ stems under less compact plant density, and in M$_2$ -stems under wide plant density, indicating same result on multiple regression analysis.

• Journal of Life Science
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• v.29 no.7
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• pp.769-773
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• 2019

In this study, the migration route and the absorption rate of calcium and magnesium in apple leaves were compared and analyzed using plant nutrients prepared by wet nano-grinding technology. The plant nutrients were sprayed onto the leaves to confirm the component content and the movement route of the nanoized calcium and magnesium. At 2, 4, and 8 weeks after the plant nutrient treatment, the apple leaves were divided into petiole, lamina, and side, and SEM and EDS were used to measure the calcium and magnesium contents. The calcium and magnesium contents of the petiole increased from the 4th week after plant nutrient application to 1,115% at the 8th week. The calcium and magnesium contents of the lamina decreased after spraying but increased after 4 weeks. The calcium and magnesium contents increased in the side of the leaves compared to the control, reaching 673% after 4 weeks. The calcium and magnesium contents increased with increasing duration in all plots when compared with the control unsprayed leaves, suggesting that the usually poorly soluble calcium and magnesium were transferred from the petioles to the lamina. The results of this study indicate that improved calcium and magnesium absorption could be obtained in crops other than apples using plant nutrients produced through wet nano-processing technology. This technology is also expected to be applicable to natural products and bioindustries.

• Plant Resources
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• v.1 no.1
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• pp.49-52
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• 1998

This study confirmed that the initiation time of tuberization was well consistent with the cativation time of JA. The consistency was also cinfirmed in the tuberization of yam plants under the altered condition of natural day length. The final yield of JA from 500g fresh leaves was $89.3{\mu}g$.

• Journal of Plant Biology
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• v.36 no.3
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• pp.297-300
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• 1993

Six flavonoid compounds from the ethyl acetate fraction of Machilus thunbergii leaves were identified by HPLC. Separation by reversed phase chromatography on u-Bondapak C18 column was achieved by isocratic elution. The contents of the major flavonoids, guaijaverin and quercitrin were about 0.48% (w/w) and 0.98% (w/w) for the methanol extract, respectively.

• The Plant Pathology Journal
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• v.20 no.3
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• pp.192-195
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• 2004

A survey on ornamental diseases in 2000 and 2001 showed frequent occurrence of blight symptoms on leaves and stems of columbine and bleeding heart in botanical gardens of Gangwon and Chungnam provinces in Korea. A total of 65 isolates of Streptobotrys sp. were obtained from the infected leaves and stems of the two plants. All isolates were identified as Streptobotrys caulophylli based on their morphological and cultural characteristics. Blight symptoms were induced on leaves of the two plants by artificial inoculation with the isolates. This is the first report that S. caulophylli causes leaf and stem blight on columbine and bleeding heart in the world.

• Mycobiology
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• v.29 no.2
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• pp.107-109
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• 2001

Anthracnose symptoms severely occurred up to 50% on leaves of English ivy growing in greenhouses in Cheongwon area of Korea during disease survey in April, 2000. The symptoms developed as concentric spots with dot-like acervuli on leaves of the plant. A total of 24 isolates of Colletotrichum sp. were obtained from the spotted lesions and identified as Colletotrichum trichellum based on the morphological and cultural characteristics. Leaf spots similar to the original anthracnose symptoms were induced on the host leaves by artificial inoculation with the isolates of the fungus. This is the first record of C. trichellum causing anthracnose of English ivy in Korea.

• Journal of Plant Biotechnology
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• v.50
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• pp.255-266
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• 2023

Hyperhydricity is a physiological anomaly that significantly affects the growth and proliferation rate of crops cultivated by tissue culture techniques. To better understand the mechanisms that govern hyperhydricity incidence, we examined the effects of several media components, particularly cytokinin and gelling agents. These elements were found to be influential in both in vitro propagation and the development of hyperhydricity. Our study revealed that Arabidopsis thaliana seedlings had a greater manifestation of hyperhydricity symptoms when exposed to high cytokinin concentrations compared with the control. The presence of gelrite led to the manifestation of hyperhydric symptoms by elevated water build-up in the apoplast. The phenomenon of stomata closure was observed in the hyperhydric leaves, resulting in an increased ability to retain water and a decrease in the transpiration rates when compared to their respective control leaves. Additionally, histological examinations of the cross sections of hyperhydric leaves revealed an irregular cellular arrangement and large intercellular spaces. Furthermore, hyperhydric seedlings displayed impaired cuticular development in comparison to their normal seedlings.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.2
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• pp.66-70
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• 1991

A field experiment was conducted to study the effects of fertilizer application rate and number of harvested leaves per plant on selected agronomic, chemical and physical characteristics of burley tobacco(Nicotiana tabacum L. cv Burley 21) in 1988, 1989 and 1990 at Chonju Experiment Station, Korea Ginseng SE Tobacco Research Institute. 175.0 or 227.5 kilogram per 10a of compound fertilizer(N-P2O5-K2O= 10-10-20) were applied as main plot, and 2 or 4 of upper leaves was topped off with 0, 2 or 4 of the lower leaves removed as sub plot. Yield, total alkaloid and total nitrogen content of cured leaves were high in the higher level of compound fertilizer plot, and increasing the level of compound fertilizer had an adverse effect on physical characteristics. Reducing the number of harvested leaves had a negative effect on yield regardless of whether the leaf number was reduced by lower topping or removing the bottom leaves, but increased the rate of higher grades leaves. Differences in total alkaloid, total nitrogen and some physical properties among number of harvested leaf per plant were smaller than expected.

• The Korean Journal of Food & Health Convergence
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• v.7 no.6
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• pp.1-8
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• 2021

Premna foetida is a woody plant with short and twisted trunk. P. foetida is a scandent, erect shrub or small tree, thorny on the trunk and large branches. Leaves are opposite or whorled and entire or serrate. Premna foetida is a wild plant locally known as "Daun Sebuas". P. foetida is used for it nutritive and as traditional treatment. The fruit and leaves of P. foetida are prepared for salad. The study aimed at the hydrodistillation and antioxidant activity of leaves, stem-bark and fruits essential oil from Premna foetida Linn, they were analysed by capillary GC and GC-MS. Ninety eight compounds representing 81.68±0.02, 37.31±0.05 and 93.45±0.03 of the isolates of leaves, stem-bark and fruits respectively were identified, the most abundant were α -Duprezianene (77.27±0.03, leaves, α-Gurjunene (36.06±0.05) fruits and Hinesol acetate (77.19±0.03) stem-bark. Components among which sesquiterpenoids dominated. The total volatiles were assayed for antioxidant potentials using 2, 2-diphenyl-1-picrylhydrazyl (DPPH). The total volatiles showed strong activity with IC₅₀ of 11.74±0.82㎍/ml, 9.63±0.34 ㎍/ml and 49.73±1.12 ㎍/ml for leaves fruits and stem-bark respectively.