• Journal of Plant Biotechnology
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• v.31 no.3
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• pp.239-248
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• 2004

This experiment was carried out to confirm that phytochrome regulates anthocyanin bio-synthesis during cell suspension culture system of grape or not. In suspension culture of grape, maximum accumulation of anthocyanin was observed at the stationary phase under continuous white light condition. From mono-chromatic light interruption for 24h at the 4th or 7th day on the suspension cultured cells, the anthocyanin accumulation was highly enhanced at the light interruption at 7th day than 4th day under all monochromatic light treatment. However, the cell growth patterns were not affected by any light treatment. In the darkness, the anthocyanin synthesis was very low but remarkably increased by blue light or red light irradiation. However, the increase of anthocyanin accumulation by blue or red light was suppressed by far-red light in the suspension cells of grape. This suppression by far-red light on the anthocyanin synthesis also observed on the cells treated red or far-red light alternatively. These results implied that phytochrome regulation system may be involved in the anthocyanin biosynthesis of the suspension grape cells. By RNA expression analysis, chalcone synthase (CHS) gene was expressed highly by blue and red light but low by far-red light. The synergistic increase of CHS gene expression was also observed at the treatment of blue light followed by red for 24h. This result may explain the increase of anthocyanin accumulation in B/R treatment. Although the expression of phytochrome gene (PHYA or PHYB) was not highly increased by all light treatment (blue, red, and far-red light) the expression of both PHYA gene and PHYB gene was increased a little in cells treated red or far-red light. In grape suspension cells, the red light enhanced the anthocyanin synthesis, whereas the far-red light was suppressed. Although it was not confirmed whether or not phytochrome gene is activated in anthocyanin accumulating grape cells, we believed that anthocyanin biosynthesis in grape cells may be regulated under phytochrome signal transduction system.

• Korean Journal of Soil Science and Fertilizer
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• v.11 no.1
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• pp.43-50
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• 1978

In order to compare the effectiveness of different phosphorus forms (pulverized as regranulized) and formulars (compound fertilizers) of fused phosphate and to compare the effectiveness of boron from different forms (straight borax, granulized with fused phosphate and included in compound fertilizer), a field experiment was conducted on a relatively fertile upland soil using soybean as a test crop. Results are summarized as following. 1. Yield of soybean was significantly higher in the plots fertilized with P when compared to check plot (no p). However, no significant differences were observed among the different forms or formulars of fused phosphate, indicating that the regranulization or formulation of compound fertilizer did not influence the effectiveness of phosphorus. 2. The application of boron, regardless of its formular, brought about higher B content in plant tissue and higher grain yield. There was a tendency that boron contained in ragranulized fused phosphate was more effective than straight borax. The results also indicated that when boron is to be included in NPK compound fertilizer, its content should be at the least 1.5% as $B_2O_3$. 3. The effectiveness of boron appeared as increased number of pod, higher B, and Ca contents in plant and the amounts of uptake of these elements by soybean. 4. The analysis of soil after the experiment indicated that different forms or formulars of fused phosphate were similarly effective in increasing the available P, Ca, and Mg contents in the soil. 5. Slight increase in available B contents in the experimental plot soils applied with boron fertilizer was observed after the trial.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.2
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• pp.209-216
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• 2010

This study was to find out optimum rice cultivars for organic farming in no-tillage paddy. A field research was conducted the yield, yield components, and qualities of thirty two Korean rice cultivars, and two Japanese rice cultivars. The column length of early rice plant was shorter in medium maturing rice cultivars compared to early, and medium-late maturing rice cultivars. Planthopper population per20-plant was lower in Ilpumbyeo, Dongjinbyeo, Hojinbyeo, Donganbyeo, and Sobeebyeo in that order while was higherin rice cultivars of early maturity. The disease severity of sheath blight was higher in Saechucheongbyeo>Chucheongbyeo>Namwonbyeo>Sangmibyeo in that order, on the other hand, that was lowerinHwaseongbyeo>Junambyeo>Saesangjubyeo=Hitomebore>Ilpumbyeo>Hwayeongbyeo in that order, respectively. The grain yield of Korean rice cultivars was significantly higher in 2.95 Mg $ha^{-1}$ for Hwasinbyeo, 2.91 Mg $ha^{-1}$ for Ilpumbyeo, 2.86 Mg $ha^{-1}$ for Sobeebyeo, 2.73 Mg $ha^{-1}$ for Naepungbyeo, and 2.70 Mg $ha^{-1}$ for Saegyehwabyeo compared with 1.38 Mg $ha^{-1}$ for Hwabongbyeo, 1.62 Mg $ha^{-1}$ for Hwayeongbyeo, and 1.78 Mg $ha^{-1}$ for Haepyeongbyeo of medium maturing rice cultivars, respectively. Toyo taste value of medium maturing rice cultivars tended to higher than that of early, and medium-late maturing rice cultivars. Ripening rate of rice cultivars was significantly positive correlated with Toyo taste value while negative correlated with protein content. According to principal component analysis, these results show that Sangmibyeo, Sangsanbyeo, and Odaebyeo for early maturity, Naepungbyeo, and Sobeebyeo for medium maturity, and Hwasinbyeo, Chucheongbyeo, and Sindongjinbyeo for medium-late maturity were optimum rice cultivars for organic farming in no-tillage paddy.

• Journal of the Korea Organic Resources Recycling Association
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• v.3 no.2
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• pp.79-89
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• 1995

Changes of microbial activity and physicochemical environment during composting of papermill sludge(PMS) in the pilot plant equipped with an agitated bed reactor were monitored for establishing the efficient composting system. Microbial activity determined as the evolution of $CO_2$ increased for the first 10 days after introduction of PMS to the reactor and decreased thereafter. Population changes of microorganisms in the reactor-PMS were not typical as in windrow system. The ratio of thermophilic bacteria to mesophilic bacteria, however, increased slowly even 23 days after introduction. Temperature of PMS increased rapidly from the first day and reached $62^{\circ}C$ at 7 days after introduction and decreased slowly thereafter. The acidity of PMS was pH 6.8 initially, increased to pH 8.0 after 7 days and decreased to pH 7.4 after 23 days. Redox potential(Eh) of PMS was -320mV at the beginning of composting, but it was increased with time to reach -15mV after 23 days composting. However, Eh of PMS pre-sterilized before measurement was average 50mV, regardless of composting periods indicating the major role of microorganisms during composting process. Water content of PMS was 67% initially and decreased to about 50% after 23 days composting in the reactor. Less than 13 days-old compost inhibited growth of radish in the container mixture with bed soil. Based on statistical analysis of microbial and physicochemical parameters of PMS during composting, an equation was developed for determining compost maturity. A number of experiments using various organic wastes are required before application of the formular to the practical use.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.63 no.3
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• pp.186-195
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• 2018

A field study was conducted over a 3-year period from 2014 to 2016 in the central plain area, Suwon, Korea, 1) to compare the biomass production between machine-transplanted and water-broadcast seeded silage rice and 2) to elucidate the growth factors that affect the difference in biomass production between the two cultivation practices. The heading date for the water-broadcast seeded silage rice was September 11-18, which was delayed by 11-17 days when compared to that for the machine-transplanted silage rice. On average, water-broadcast seeded silage rice had a shorter plant height, more panicles per area, and a greater biomass production because of the increased straw dry weight. However, the difference in dry weight of each plant organ between the two cultivation practices exhibited yearly variation. When the data were pooled across experimental years, cultivation practices, and varieties, biomass production was highly positively correlated with straw dry weight but not with panicle dry weight. When the ratio of water-broadcast seeding to machine-transplanting was analyzed, total dry weight and straw dry weight were positively associated with each other, whereas no relationship was found between total dry weight and panicle dry weight. Despite that water-broadcast seeded silage rice produced more panicles per area than machine-transplanted silage rice, the two cultivation practices had a similar dry weight per culm. Therefore, we conclude that the silage rice from the water-broadcast seeding, compared to the machine-transplanting, produced more biomass because of the combination of the increased panicle number per area and a similar dry weight per culm. These results suggest that silage rice could be produced through water-broadcast seeding to increase biomass production with low labor and cost input.

• Korean Journal of Environmental Biology
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• v.34 no.2
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• pp.63-72
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• 2016

The objective of this study was to identify the life form characteristics, naturalized degree and introduction period of naturalized plants on upland fields in South Korea. The survey sites were selected in 222 upland fields over 9 provinces where are relatively dry soil conditions and are disturbed continuously by farming practices. It seemed that these uplands maintain good condition for the invasion and settlement of alien plants. Field surveys were carried out twice from May to June and from August to September in 2013. The vascular plants of the upland fields were listed into 539 taxa which contain 103 families, 320 genera, 448 species, 2 subspecies, 74 varieties and 15 forms. Ninety nine taxa were naturalized plants composed of 23 families, 64 genera, 91 species and 8 varieties. The urbanization index and naturalized index is 30.65% and 18.37%, respectively. Among total 99 naturalized plants, Compositae (30 taxa) was the most dominant family, and followed by Gramineae (14 taxa). On the basis of dormancy form, 78 taxa were belong to Th (Therophyte). In radicoid form, $R_5$ plants (86 taxa) and $R_3$ plants (14 taxa) were dominantly distributed. In disseminule form, $D_4$ including 63 taxa and $D_2$ containing 26 taxa were dominant. On the basis of growth form, erect form (e) containing 26 taxa was dominant. In conclusion, life form spectrum was $Th-R_5-D_4-e$ type. According to life form analysis, naturalized degree and introduction period, alien plants with unique characteristics may have high ability to settle and spread nationwide after inflow into South Korea. It needs further to observe some plant species with or without ecological features such as widely disseminated seeds by wind or water, high seed productivity, long life-span seeds, high germination rate of seeds, or non-dormant germinable seed.

• Korean Journal of Environmental Biology
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• v.30 no.2
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• pp.90-97
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• 2012

Environmental characteristics and vegetation of the natural habitats of Eranthis byunsanensis B.Y. Sun were investigated in order to provide the basic data for conservation, restoration, and utilization of this Korean endemic plant. This study was conducted in Anyang, Byeonsan, Geoje, Gyeongju, Jeju, Ulsan and Yeosu. E. byunsanensis was distributed around the altitudes of 84~585 m with a slope degree of $10{\sim}20^{\circ}$, and mostly formed discontinuous populations in north-east part of valleys. Soil analysis showed the mean organic matter of 9.6% and a slightly acidic pH (mean pH of 4.9). The mean gravimetric water content was 16.5%. Correlation coefficients between environmental factors and community characteristics suggested that there was a positive correlation between slope degree and soil water content, between slope degree and soil pH, between soil organic matter and importance value, and between species richness or evenness and species diversity. The vascular plants from 59 quadrats of 7 habitats were identified into 144 taxa. A few species were dominants and similarly distributed in Byeonsan, Jeju, Ulsan and Yeosu. The highest species diversity was found in Geoje (1.43), while Anyang showed the lowest (0.87). Species evenness of Gyeongju and Jeju was bigger than 0.8, but that of Geoje was the lowest (0.59). Dominant species of woody plants in and around the 59 plots were represented by high frequency of Acer pictum subsp. mono, Carpinus cordata, Lindera obtusiloba, and Carpinus laxiflora. The results of this study can provide useful data for conservation and restoration of natural habitats of Korean endemic Eranthis byunsanensis and for the development and growth of this species for ornamental purposes.

• Korean Journal of Environment and Ecology
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• v.32 no.2
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• pp.165-175
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• 2018

The purpose of this study is to provide the basic data such as distribution status, growth characteristics, and the structural characteristics of forest stands for the systematic conservation and management of relict forest trees (stem girth of 300cm or larger) established naturally in Gangwon Province, Korea. The survey showed that 434 individuals of 19 species (conifers: 228 individuals of 4 species, broad-leaved trees: 206 individuals of 15 species) were distributed in Gangwon Province, and Taxus cuspidata was the most abundant among them with 203 individuals or about 46.7 % of the total. The stem girth was average of 404cm (conifers: 373cm, broad-leaves: 421cm), and Tilia amurensis with multi-stemmed growing on Sorak mountain range had the largest stem girth at 1,113cm. The average height and the crown width of relict forest trees were 15.4m and 10.0m, respectively. Although the environments of relict forest trees showed a slight difference by species, the relative appearance frequencies of most trees were high in the environments where the altitude was higher than 1,000 m, slope degree was greater than $25^{\circ}$, the slope faced north, and microtopography was at the upper of slopes. Regarding the stand characteristics of relict forest trees per unit area ($/100m^2$), the average total coverage was 294% (max. 475%), the total average number of species was 36 species (max. 60 species), the average species diversity index (H') was 2.560 (max. 3.593), the average canopy closure was 84.8% (max. 94.6%), and the average basal area (/ha) was $52.7m^2$ (max. $116.4m^2$, relict trees $30.0m^2$, and other trees $22.7m^2$). The analysis of the dynamics of the forest stands where relict forest trees were growing showed four types of the maintenance mechanisms of relict forest trees depending on the supply pattern of succeeding trees: "Low-density but persistent type (Quercus mongolica, Abies holophylla, Tilia amurensis, and Pyrus ussuriensis)," "Long ago stopped type (Pinus densiflora)," "Recently stopped type (Abies nephrolepis, Quercus variabilis, and Betula schmidtii)," and "Periodically repeated types of supply and stop (Salix caprea and Quercus serrata).".

• Journal of Soil and Groundwater Environment
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• v.11 no.3
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• pp.43-51
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• 2006

Generally abandoned mine soils have serious problems for introducing vegetation such as nutrient deficiency, poor physical properties, and phytotoxicity due to high levels of heavy metals. It is required to improve soil amenity for revegetation. One of its strategies is using organic materials such as compost manure and sludge. The pot experiments was conducted to evaluate the effects of pig manure and municipal sewage sludge on revegetation of mining area soil surface with Artemisia princeps and Zoysia japonica. Application rate of pig manure and municipal sewage sludge was $75{\sim}225$ Mg/ha and $150{\sim}450$ Mg/ha, respectively. The results showed that the application of manure and sludge increased organic matter about two-fold and total nitrogen contents about five-fold of mine soil and improved the growth of plants in all treatments compared to the control. The result of plant tissue analysis showed that both plants accumulate Cd, Cu and Zn in root tissue rather than shoot tissues. Increased sludge application reduced Zn accumulation in both plant tissue. Sequential extraction results indicated that addition of soil amendment induced increment of organically bound fractions of Cu and Zn. Organically bound fraction of Zn was significantly increased from 7.84% to 13.58% in Artemisia princeps planted soil and from 7.84% to 14.16% in Zoysia japonica planted soil, thereby bioavailability of heavy metals was reduced. The results suggested that application of organic materials to mine soil can reduce phytotoxicity of heavy metals and be helpful in introducing successful revegetation.

• Horticultural Science & Technology
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• v.29 no.1
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• pp.29-35
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• 2011

Individual ion concentrations and ionic contributions to EC reading in the circulated nutrient solution are the important factors to be considered for stable EC-based closed-loop soilless culture. This study was conducted to determine appropriate ion-analysis intervals of the circulated nutrient solutions based on ion concentration, ion balance, and ion electrical conductivity under different renewal intervals in EC-based nutrient control systems for sweet peppers (Capsicum annum L. 'Fiesta') in early growth stage. Average node numbers of the plants were 13 and 18 when the experiment started and finished, respectively, and three plants were grown in each rockwool slab. Four different renewal intervals of circulated nutrient solutions such as 1, 2, 3, and 4 weeks were used as treatment. Nutrient solutions were supplied to the plants based on integrated radiation. Drainage was collected into drain tanks after irrigation ended in the day and then mixed with fresh water until the EC reaches 2.69 $dS{\cdot}m^{-1}$. The replenished nutrient solution was supplied to the plants in the next day. Ion concentrations of the individual ions periodically analyzed in the circulated nutrient solutions showed no significant differences among the treatments during the experimental period. Ion concentrations of $K^+$, $Ca^{2+}$, $Mg^{2+}$, $Na^+$, $NO_3{^-}$, ${SO_4}^{2-}$, ${PO_4}^{3-}$, and $Cl^-$ varied within 5-8, 11-14, 2.0-2.7, 0.5-0.6, 14-19, 4-5, 1-4, and 0.3-0.5 $meq{\cdot}L^{-1}$, respectively. Ion balance showed a consistent tendency over all the treatments and especially $K^+$ : $Ca^{2+}$ and ${SO_4}^{2-}$ : ${PO_4}^{3-}$ played great roles in the cation and anion balances in the nutrient solutions, respectively. Activity coefficients of ions such as $K^+$, $NO_3{^-}$, and $H_2PO_4{^-}$ varied within 0.8-0.9 and those of $Ca^{2+}$, $Mg^{2+}$, ${SO_4}^{2-}$ varied within 0.5-0.6, showing little changes with time. Ionic contributions of $K^+$ and $NO_3{^-}$ to EC reading were the greatest followed by $Ca^{2+}$, ${SO_4}^{2-}$, and $Mg^{2+}$ in the order. From the results, we thought that allowable ranges in ion concentration, ion balance, and subsequent individual ionic contributions to EC reading would be obtained within 4-week renewal interval of nutrient solution in EC-based closed-loop soilless culture for sweet pepper plants.