• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.60-60
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• 2023

특허제도는 발명자에게 발명의 공개에 대한 대가로 일정기간 독점권을 인정하여 기술개발에 투자한 자금을 회수할 수 있는 기회를 제공하고 공개된 발명은 새로운 기술개발을 위하여 누구나 이용할 수 있도록 하여 기술발전을 촉진할 뿐만아니라 이미 개발된 발명에 대한 중복투자, 연구를 하는 낭비를 방지하기 위한 목적을 가진다. 본 조사는 2022년 12월 31일까지의 정부 특허정보넷인 키프리스(www.kipris.or.kr)에서 꽃차 관련 실용신안 특허 내용을 검색하여 정리하였으며 그 결과는 다음과 같았다. 1. 1983년 엘다베리꽃차 제조방법에 대한 것을 시작으로 최근 2022년 12월 31일까지 총 30개가 출원되어 2022년에는 증가된 것이 없었다. 2. 출원된 실용신안 특허 총 30개 중에서 소멸 2개(엘다베리/1983, 연꽃/2009), 취하 3개(무궁화/1992, 백화차/2005, 꽃차/2014), 거절 6개(매화/2001, 장미녹차/2007, 혼합꽃녹차/2007, 동결꽃차/2008, 소금꽃차/2015, 꽃차누룽지/2017), 공개 6개(꽃차/2014, 소금꽃차/2015, 꽃차추출물/2017, 기능성꽃차 /2018, 꽃차추출물/2018, 금잔화꽃차/2019), 포기 2개(개복숭아 꽃차/2015, 동백꽃차/2017), 등록(녹차꽃차/2006, 사과꽃차/2008, 목련꽃차/2012. 목련꽃차/2014, 칡꽃차/2015, 장미꽃차/2017, 모란꽃차/2017, 꽃차제조방법/2018, 꽃차로스팅장치/2018, 꽃음료/2020, 향 강화 꽃차/2021)은 11개였다. 3. 출원된 실용신안 특허 총 29개 중에서 개인이 출원한 것이 19개, 회사(아모레퍼시픽, 휴먼코스메틱, 화이통협동조합, (주)꽃다비, 주식회사 플러드, (주)우리꽃연구소)나 기관(한국식품연구원, 고창군), 대학(전남대, 동의대 2개)이 출원한 것이 11개였다.

• Bulletin of the Korean Chemical Society
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• v.33 no.8
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• pp.2609-2612
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• 2012

A simple green method was developed for rapid synthesis of silver and gold nanoparticles (AgNPs and AuNPs) has been reported using Lonicera japonica flower extract as a reducing and a capping agent. AgNPs and AuNPs were carried out at $70^{\circ}C$. The successful formation of AgNPs and AuNPs have been confirmed by UV-Vis spectro photometer, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), energy dispersive X-ray Analysis (EDAX), scanning electron microscopy (SEM) and high-resolution transmission electron microscopy (HRTEM). To our knowledge, this is the first report where Lonicera japonica flower was found to be a suitable plant source for the green synthesis of AgNPs and AuNPs.

• Proceedings of the Botanical Society of Korea Conference
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• 2002.04a
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• pp.112-112
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• 2002

• The Korean Journal of Ecology
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• v.19 no.2
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• pp.107-123
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• 1996

The position effects on the abortion of flowers, fruits, and ovules were examined in relation to the resource limitation hypothesis using a temperate legume species, Vicia cracca. Fifty plants were randomly sampled from a natural population located at the Belmont Conservation Site in Belmont, MA, USA. Due to indeterminant growth and acropetal flowering of this species, the position of flower or fruit on the plant was able to be coded by the combination of architectural levels such as plant half (lower and upper half) and inflorescence half (lower and upper half) within each plant half. Overall, only 17.3% of the flowers on an inflorescence began to develop into fruits, while overall 36.0% of the fruits initiated failed to mature. Consequently, 11.5% of the flowers successfully matured into fruits. The mean flower number per inflorescence differed significantly among plants as well as between plant halves. Although the probability of fruit initiation was higher in the lower halves of both plant and inflorescence than in the upper halves of the latter, fruit abortion rate differed significantly only between plant halves. The overall mean seed set was 33.6%, implying that about two out of six ovules in a fruit matured into seeds. The seed set was independent of both plant identity and architectural level unlike other reproductive characters examined. When the ovule developmental stage was examined on data pooled over all fruits, the proportion of ovules in varying developmental stages decreased in order of early abortion, seed maturation, late abortion, and seed predation (48.4, 34.9, 12.2, and 4.5%, respectively). A within-fruit ovule position was also used as a class variable for the analysis of position effects on ovule development. All architectural levels considered exerted singificant influence over the ovule development. In particular, ovule development was strongly affected by the within-fruit ovule position. Ovules in both extreme ends within a fruit tended to abort early, while those in the middle position were more likely to mature into seeds. The strong position effects detected from the flowering to seed maturation stage were interpreted as an indication of competitive interaction among reproductive characters which are largely constrained by plant architecture.

• Journal of Plant Biotechnology
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• v.34 no.4
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• pp.293-298
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• 2007

Robinia pseudoacacia var. umbraculifera, commonly called umbrella black locust were regenerated after co-cultivation of internode segments with Agrobacterium tumefaciens which included yeast cadmium factor 1 (YCF 1) gene. The tolerance to cadmium and lead for plants can be increased by the YCF1 gene expression. Moreover, the recent studies have shown that YCF1 gene transgenic plants increase the accumulation of cadmium and lead into plant vacuoles. The effect of plant growth regulator such as 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}$-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron (TDZ) were studied to evaluate the propagation of plants through internode explants. The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA. To induce shoot elongation and rooting, regenerated shoots were transferred into basal MS medium without any plant growth regulator. Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime. To confirm the integration and expression of transgene, Polymerase Chain Reaction (PCR) and Reverse Transcriptase PCR (RT-PCR) were performed with specific primers. The frequency of transformation was approximately 18.94%. This study can be used to genetic engineering of phytoremediator.

• Horticultural Science & Technology
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• v.16 no.1
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• pp.37-39
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• 1998

This study was carried out to investigate the effects of nutrient solution concentration, irrigation frequency on growth, flowering, and cut flower longevity of Dendranthema grandiflorum (Ramat.) Kitamura 'Shuhouno-chikara' hydroponically grown in perlite media. Not only stem length but also mineral contents of each plant organ in nutrient solution culture were much higher than those in soil culture. 1/2S of nutrient solution was good at early stage, but 1S of nutrient solution was better as chrysanthemum growth progressed. Among different concentrations of nutrient solution, mineral contents of each plant part showed no significant difference. 1S treatment of nutrient solution increased the vase life by 3 days than 2S treatment. The growth and mineral contents of each plant organ were great in plants irrigated 8 times a day, because of high moisture contents of medium and high water availability, followed by more frequent nutrient replenishment near roots. There was no correlation between nutrient solution concentration and vase life of cut flower grown in nutrient solution culture. Cut flowers irrigated twice per day had the longer vase life than other treatments (4, 8 times).

• Korean Journal of Agricultural Science
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• v.11 no.1
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• pp.77-84
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• 1984

This experiment was carried out to determine the effects of night temperature and amount of fertilization on growth and photosynthesis of red pepper plants for providing some basic imformation needed in improving the productivity of red pepper. 1. Plant height, stem length, number of flower buds, dry weight of plants and photosynthetic rate were higher in King Gun Gochu than in Dohusa, but number of leaves, number of internodes and leaf area we re higher in Dohusa compared to King Gun Gochu. 2. Plant height, number of leaves, number of flower buds, dry weight of plants, leaf area and photosynthetic rate were significantly increased by 50% increased fertilization compared to normal fertilization. 3. High night temperature($25^{\circ}C$) treatment increased plant height, stem length, number of leaves, number of internodes, number of flower buds, dry weight of plants and leaf area. 4. RGR and LAR were increased by 50% increased fertilization and high night temperature but NAR was decreased by high flower buds 5. Photosynthetic rate of King Gun Gochu was increased by 50% increased fertilization and high light intensity. 6. Number of flower buds was increased at King Gun Gochu by 50% increased fertilization and high night temperature ($25^{\circ}C$).

• Korean Journal of Plant Resources
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• v.28 no.5
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• pp.648-655
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• 2015

Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.

• FLOWER RESEARCH JOURNAL
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• v.17 no.1
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• pp.15-22
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• 2009

This study was conducted to examine characteristics of rhizome growth and flowering of white lotus (Nelumbo nucifera) plant in Muan Hoesan-lake for collecting the basic data on the cultivation and the flowering which is growing in Muan Hoesan-lake of Jeonnam province in Korea. We have planted from the seeds and the enlarged rhizomes of the white lotus which were planted in the plastic containers on April 20 and May 4, 2008, and cultivated under rain shelter condition. Rhizome growth pattern and the flowering characteristics of the white lotus were examined on September 4, 2008. Main rhizomes of the white lotus propagated by the seeds and the enlarged rhizomes were branched by 10% and 15%, respectively. Numbers of nodes in the bloomed and non-bloomed rhizomes of the white lotus were over 14 and below 11, respectively. Flower was not observed in the white lotus when propagated with seeds, however, flowers came out up to 80% by the enlarged rhizomes. First flowers were bloomed from the late July and to the early August, and the number of flowers were 4.0~4.4. The first flower stalks without branching of the main rhizome were emerged in 8.8th node, however, that with branching were 8.0th node. Time to flowering from the emergence of flower stalk on the above-ground parts were required 16 days. White flowers from the lotus were 15.3 cm in height and 28.2 cm in width, their longevity was 3.3 days and the averaged flower fresh weight was 29.2 g. The results conclude that optimum number of nodes on the main rhizomes for blooming the white lotus flowers should be over 8 nodes before the rhizome enlargement.

• Korean Journal of Organic Agriculture
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• v.20 no.2
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• pp.243-258
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• 2012

These grafting seeding "Torobambica", rootstock "Chuk Yang" were tested to investigate the growth, yield, and quality of eggplants which can be influenced by the bumblebee pollination and plant growth regulators. The results were as follows: According to the treatment of bumblebee pollination and plant growth regulators, the yield of eggplant was good at bumblebee pollination with tomatotone flower spray at blooming time, and was good at foliage spray on the treatment of tomatotone and $GA_3$, and was good at flower clusters spray on the treatment of tomatotone. According to the treatment of bumblebee pollination and plant growth regulators, the ratio of marketable fruit was good at pollination bumblebee with tomatotone spray on the day they flowered, was good at foliage spray on the treatment of tomatotone and $GA_3$, and was good at flower clusters spray on the treatment of tomatotone. On the mineral element contents of eggplant stems, the contents of total nitrogen were good at tomatotone flower spray at blooming time, the ones of phosphorus, potassium, and magnesium were good at tomatotone foliage spray the rate of three days, the ones of calcium were good at tomatotone flower spray in two or three days before flowering, and ones of ash were good at tomatotone flower spray at blooming time, foliage spray the rate of three days. On the mineral element contents of eggplant fruits, the contents of total nitrogen were good at tomatotone and $GA_3$ foliage spray the rate of six days, the ones of phosphorus were good at tomatotone foliage spray the rate of three days, the ones of potassium, ash were good at tomatotone and $GA_3$ flower spray at blooming time, and the ones of calcium, magnesium were good at tomatotone an $GA_3$ flower spray in two or three days before flowering.