• Korean Journal of Plant Tissue Culture
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• v.22 no.5
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• pp.273-276
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• 1995

In vitro propagation of Neoregeria carorinae 'Tricolor' was achieved by using immature flowers and lateral buds, and the plantlets from tissue culture were transplanted and cultivated in greenhouse. The picking times of explants to decrease disappearance of stripes, and in vivo the growth and flowering of regenerated plantlets as influenced by in vivo healed nun were investigated. The normal plantlet were obtained at a frequency of 67%, in the culture of immature flowers picked at 4 weeks after flower bud differentiation, while all leaf stripes disappeared in the culture of immature flowers picked 1 and 5 weeks after flower bud differentiation. In vivo growth of plantlet from immature flower buds was better than those from lateral buds, and the flowering of 27.8% showed in the greenhouse culture of plantlet from immature culture, but the plantlets from lateral buds did not flower at all. The plantlets rooted on the medium with 0.5 mg/L IBA were the most favorable in green house culture, and the kinds and concentrations of auxin in vitro did not have any influence on variation of plane cultured in greenhouse.

• Korean Journal of Plant Resources
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• v.33 no.3
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• pp.153-162
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• 2020

The root extracts of Paeonia lactiflora cv. 'Red Charm' has been studied by many groups, however, little attention has been paid to its flower petal. Paeonia is the genus in the Paeoniaceae family. 'Red Charm' Paeonia is a soft-stemmed herbaceous peony hybrid of P. officinalis and P. lactiflora. We previously showed the flower petal extract of Red Charm might have anti-oxidant and anti-inflammatory activities, however, it was not clear which components might be involved in this activity. Bioinformatics analysis previously indicated these extracts have potential anti-oxidant materials. One of them is identified as paeoniflorin, which is major component in root extract of Red Charm. In this study, we compared paeoniflorin and oxypaeoniflorin using DPPH assays to measure its anti-oxidant activities. Oxypaeoniflorin showed higher levels of radical scavenging activity, similar to ascorbic acid control, whereas paeoniflorin did not. Furthermore, nitric oxide assay showed they have similar anti-inflammatory effects. Taken together, these results suggest oxypaeoniflorin may play a more important role in the anti-oxidant activity of the flower petal and root extracts of Red Charm, compared to paeoniflorin. Further studies may be able to provide a platform to develop potential dual effects therapeutics for oxidant-mediated and inflammation-mediated disease in the near future.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.144-149
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• 2006

This is a study on relationship between soil pH and flower color and growth of Hibiscus siriacus L, looking for the pH range to result the most ideal flower color. I anticipate this study result will lay a foundation of improved breeding plan of Hibiscus siriacus L. According to the result of investigation and analysis on bleeding peculiarities by each soil pH, the numbers of leaves in Honghwarang and Younggwang reached the highest at pH 7: 188.73 and 135.87 respectively. Increased pH resulted the increased number of leaves. According to the result of investigation and analysis on color changes of four kinds of Hibiscus siriacus L. at various pH level, Honghwarang's L value gradually decreased from 17.05 to 12.26 at pH increase while a value increased from 16.37 to 20.91.

• Korean Journal of Plant Resources
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• v.8 no.3
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• pp.265-274
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• 1995

Characteristics of vegetative spur leaf, spur bud, fruiting supr leaf and flowering in relation to canopy position was investigated on order to promote better spur bud and flower bud differentiation on Citrus junos. There was no difference in vegetative spur leaf characteristics among directions. Vegetative spur leaf number and leaf area showed no difference between top and bottom position. There was no difference in spur bud size among directions, but spur bud at top was larger than that at bottom. Specific leaf weight of vegetative spur was mostly influenced by light interception, and leaf dry weight per spur average leaf dry weight, spur bud length and diameter had also a very high correlation with light interception rate. Shading and GA treatment in spur bud and flower bud differentiation was ineffective than natural light. Fruit thinning enhanced fruit quality as well as flower bud differentiation through an increase of leaf number per fruit, over 40 in Citrus junos.

• Proceedings of the SCSK Conference
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• 2003.09b
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• pp.32-43
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• 2003

UV irradiation on a skin brings about the qualitative and quantitative alterations of the extracellular matrix. Repeated-UV irradiation suppressed the synthesis of collagen and activated the expression of the matrix metalloprotease (MMP). In this paper, on the purpose of development of novel anti-aging agents from natural sources, effects of several natural products on in vitro MMP-1 activity and UVA induced MMP-1 synthesis in human dermal fibroblast (HDF) culture were studied. We measured MMP-1 activities by fluorescence assay using gelatin as substrates. As a result, the extract of Dicentra spectabilis, and flower buds of Tussilago farfara showed strong inhibitory effect. Among them, the extract of flower buds of Tussilago fartara and Dicentra spectabilis inhibited MMP-1 activity by 92% and 87% at 0.05% (w/v). And UVA induced MMP-1 expression were analyzed by enzyme-linked immunosorbent assay (ELISA) and gelatin-based zymography in HDF culture. The extract of flower buds of Tussilago farfara and Dicentra spectabilis suppressed the UVA induced expression of MMP-1 by similar level of Vitamin C 200$\mu$M at 0.1% (w/v). These results suggest that the extract of Dicentra spectabilis, and flower buds of Tussilago farfara effectively prevent skin from the UV-induced photoaging. So the extracts are thought to have potential as effective raw materials for anti-aging cosmetics.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2001.04b
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• pp.45-50
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• 2001

Stem firmness and flowering response of cut lily as influenced by medium composition (Control: Upland soil, Pt: Peatmoss, Pe: Perlite, Ve: Vermiculite, Rrh: Rotted rice-hull, RPt: Russian Peatmoss) were studied. For 'Casa Blanca', plant height and length of flower stalk increased when bulbs were planted in Pt:Rrh:Ve(1:1:1, v/v), and dried leaves of lower part plants decreased by RPt:Pe:Rrh(1:1:1, v/v). In case of 'Marco Polo' plant height and length of flower stalk increased with Pt:Rrh(1:1, v/v) as compared to other treatment, number of leaves and dried leaves increased when bulbs were planted in RPt:Pe:Rrh(1:1:1, v/v) as compared to control. Flowering of 'Casa Blanca' was promoted in Pt:Pe:Ve(1:1:1, v/v) and 'Marco Polo' was accelerated in Pt:Rrh:Ve(1:1:1, v/v). Flower length of 'Casa Blanca' was increased by RPt:Pe:Rrh(1 :1 :1, v/v) as compared with control and 'Marco Polo' was increased when bulbs planted to Pt:Rrh(1 :1, v/v). Flower-bud blasting of two cultivars was increased with Pt as compared with other treatment. Stem firmness of 'Casa Blanca' was increased by Pt:Pe:Ve (1:1 :1, v/v), and especially, stem firmness of upper part plants was increased by Pt:Rrh(1 :1, v/v) in 'Marco Polo' as compared to control plants. but generally, stem firmness of 'Casa Blanca' was not influenced with all cultural media as compared to control.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.185-189
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• 2009

This study was carried out to investigate the effect of shading (50% or not) and pruning height (3cm, 6cm, and 9cm) of remained above-ground plant part on shoot emergence aspect, mortality rate, flowering and cut flower characteristics of two years old Gypsophila paniculata plants. Days to shoot emergence (80%) advanced, emerged shoot number increased and mortality rate surveyed on late October decreased in 50% shading treatment. In addition to, mortality rate decreased, as above-ground plant part was highly pruned. Blooming was earlier with highly pruning the remained above-ground pan and under non-shading condition than shading. Growth traits such flower stalk length, primary branches number, stem diameter, and cut flower yield were the best and also non-particulate rate and rosette formation rate were the lowest in pruning height of 9cm under non-shading condition.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.52-52
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• 2019

Buckwheat is well-known crop for containing a high contents of flavonoids that are effective in vascular disease. The current study was performed to estimate the influence of morphological characterization of Fagopyrum esculentum (ES) germplasm for seed's two major flavonoids contents: rutin and quercetin. We found that the red stem color, pale green leaf color, arrowhead leaf shape, white flower color, pale brown seed coat color, and egg-shaped seed were significantly associated with 77%, 56.7%, 83.7%, 98.7%, 70.8% and 74.5% germplasm, respectively. Overall, the rutin contents of ES germplasm ranged from 0.30 to 47.86 mg/100g dry weight (DW) and the quercetin contents ranged from 0 to 1.22 mg/100g DW. The rutin contents of germplasm possessing red stem color, pale green leaf color, arrowhead leaves, white flower color, pale brown seed coat color and egg-shaped seed ranged from 7.22 to 47.86 mg/100g DW. However, the quercetin contents of germplasm with red stem color and pale brown seed coat color ranged from 0 to 1.15 mg/100g DW, with pale green leaves ranged from 0 to 0.96 mg/100g, with arrowhead leaves and white flower ranged from 0 to 1.22 mg/100g and with egg-shaped seed ranged from 0.32 to 1.22 mg/100g DW. In PCA analysis, the first three principal components (PCs) showed Eigen value more than 1 and accounted for 51.70% of variation. For both higher contents of rutin and quercetin, the morphological evaluation in ES shows a tendency of red stem color, arrowhead leaves, pale green leaf color, white flower color, pale brown seed coat color and egg-shaped seed. From this information, we can assume the rutin and quercetin contents by the morphological characteristics of the germplasm. And It could be useful in improving the rutin and quercetin contents and selecting proper resources for cultivation in existing buckwheat cultivars.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.109-109
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• 2020

꽃차(Flower tea)는 최근 다양한 제품이 개발되고 소비가 확대되는 등 그 산업적 가치가 증대하고 있다. 꽃차의 향기 특성은 우렸을 때 나타나는 색깔 및 인체에서의 생리활성과 더불어 주요한 꽃차 품질결정 요소이다. 본 연구는 꽃차로의 이용이 활발한 국화과 식물 중 구절초(Dendranthema zawadskii var. latilobum), 국화(Chrysanthemum morifolium), 노랑색 및 주황색 마리골드(Tagetes erecta 'Yellow' and 'Orange'), 그리고 캐모마일(Matricaria chamomilla)의 향기 성분특성을 구명하기 위하여 제조된 꽃차를 headspace-solidphase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS)를 이용하여 분리, 동정하였다. 국화과 꽃차로부터 총 117종의 휘발성 성분이 확인되었는데, 각 꽃차 종류별 동정된 휘발성 성분의 개수, 주요 3개 휘발성 성분과 전체 향기성분 peak중 이들이 차지하는 구성비율(%)은 다음과 같았다: 구절초 64종, camphor (31%), α-pinene(14%), camphene(14%); 국화 60종, camphor(15%), chrysantheny acetate(13%), eucalyptol (11%); 마리골드 '옐로우' 53종, 2,4-heptadienal(26%), trans-isocarveol(21%), cis-β-Copaene(18%); 마리골드 '오렌지' 61종, β-caryophyllene(16%), β-ocimene epoxide(12%), β-ocimene(12%); 캐모마일 50종, β-farnesene(63%), nonane(9%), spathulenol(5%). 국화과 꽃차 5종 모두에서 공통적으로 검출된 성분은 β-caryophyllene, α-pinene, β-farnesene 등 10종이었으며 마리골리 '옐로'는 '오렌지'와 주요 향기성분의 조성에서 뚜렷한 차이를 나타내었다. 비록 그 함량은 낮았으나 구절초, 국화, 마리골드 '오렌지', 그리고 캐모마일은 각각 10종, 12종, 3종 및 13종이었다. 마리골드 '엘로'의 경우 검출된 모든 향기성분은 마리골드 '오렌지'나 다른 국화과 식물의 꽃차에서도 검출된 바, 향기 성분이 다양성이 다소 낮게 나타났다.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2020.08a
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• pp.110-110
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• 2020

꽃을 우려내어 차로 마시는 꽃차(flower tea)는 꽃이 가진 색과 향, 맛과 모양을 즐길 수 있어 최근 수요가 급증하고 있다. 본 연구는 매화(Prunus mume), 장미(Rosa spp.), 그리고 해당화(Rosa rugosa) 등 3종의 장미과 식물의 꽃차의 향기 성분 특성을 구명코자 꽃차를 headspace vial에 담은 후 solid phase microextraction(SPME)를 이용하여 추출한 후 gas chromatography-mass spectrometry를 이용하여 휘발성 성분을 분리하였다. 분리된 각 성분은 deconvolution 과정을 수행한 후 NIST masss spectral library를 이용하여 동정하였다. 매화꽃차에서는 총 58개의 휘발성 성분이 검출되었는데, benzaldehye와 nonane, 그리고 phenylmethanol이 주요 구성 성분으로 검출된 전체 휘발성 성분의 각각 64.7%, 16.1% 및 4.7%를 차지하고 있었다. 장미 꽃차의 경우 검출된 35종 중 주요 구성 성분은 nonane과 2-phenylethanol, 그리고 phenylmethanol로서 각각 전체 성분 중 54.0%, 18.0% 및 4.6%를 구성하고 있었다. 해당화 꽃차의 경우는 장미 꽃차와 동일하게 2-phenylethanol(62.2%)과 nonane(20.1%)이 주요 성분으로 동정되었으며 이들 2 성분의 합이 전체 43개 휘발성 성분의 82.3%를 차지하는 것으로 나타났다. 본 연구에 사용된 매화, 장미, 해당화 등 장미과 식물 3종의 꽃차 모두에서 공통적으로 검출된 휘발성 성분은 앞서 각 꽃차 종류별 주요 성분으로 언급된 4종의 성분을 포함하여 19종이었으며 매화꽃차에서만 검출된 성분은 29종으로 장미꽃차(6종)와 해당화 꽃차(8종)보다 향기성분의 다양성이 높은 것으로 나타났다.