• Title/Summary/Keyword: Plant collection

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Inheritance of Apiculus Color, Awn, and Long Empty Glume in Korean Rice Collection

  • Kang, Hee-Kyoung
    • Plant Resources
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    • v.6 no.2
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    • pp.98-101
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    • 2003
  • Colored apiculus, awn, and long empty glume are indicators of wildness and are usually eliminated during rice domestication. Genetic analysis was conducted to clarify the inheritance patterns of awn, apiculus color, and long empty glume in Korean rice collection. Based on individual characterization of F$_2$ progenies derived from crosses between parents with colorless and purple apiculus, two (3 colored: 1 colorless) or three dominant genes (9 purple: 3 red: 4 colorless) are estimated as controlling this character by simultaneous complementary action. Different inheritance systems were detected between S237 and S245 of 'Shareibyeo' which belong to the weedy type. To determine the genes responsible in awning and long empty glume characters, the inheritance of landrace varieties of rice ('Naengdo' and 'Yuna') was investigated. In the crosses of awned land race and awnless cultivar, three dominant genes are supposed to control the awning genetic system by 63 awned: 1 awnless individual. As for long empty glume, one recessive gene, g-l on the chromosome 4, was the one controlling the segregation ratio of 3 normal empty: 1 long empty glume. By analyzing the Korean rice collection, the inheritance systems of these wild characters may lead to a better understanding of rice domestication in the future.

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Comparisons of Recombinant Protein Expression in Diverse Natural Isolates of Escherichia coli

  • Jung, Yuna;Lim, Dongbin
    • Molecules and Cells
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    • v.25 no.3
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    • pp.446-451
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    • 2008
  • We assessed heterologous protein expression in 64 strains obtained from the Escherichia coli Reference (ECOR) collection, a collection representing diverse natural E. coli populations. A plasmid generating a glutathione S-transferase and plant carbonic anhydrase fusion protein (GST-CA) under the control of the tac promoter was introduced into the ECOR strains, and the quantity of the fusion protein was determined by SDS-PAGE. The foreign protein was generated at various levels, from very high (40 strains, high producers) to very low (six strains, low producers). Immunoblotting showed that the high producers expressed approximately 250-500 times more GST-CA protein than the low producers. The results of semi-quantitative RT-PCR showed that the low producers generated mRNA levels comparable to those of the high producers, thereby suggesting that, at least in this case, inefficient translation is a major cause of the low production. We introduced a different plasmid, which expressed a maltose binding protein and plant guanylate kinase fusion protein (MBP-GK) into the six low producers. Interestingly, five of these expressed MBP-GK at very high levels. Thus, we conclude that the production of a particular protein from an expression vector can vary considerably, depending on the host strain. Strains in the ECOR collection could function as useful alternative hosts when a desired level of protein expression is not obtained from commonly used strains, such as E. coli K12 or B derivatives.

Antibiotic Effect of Leaf, Stem, and Root Extracts in Smallanthus sonchifolius H. Robinson

  • Shin, Dong Young;Hyun, Kyu Hwan;Kuk, YongIn;Shin, Dong Won;Kim, Han Woo
    • Korean Journal of Plant Resources
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    • v.30 no.3
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    • pp.311-317
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    • 2017
  • This study was conducted with the aim of evaluating the antibiotic effects of leaves, stems, and roots in yacon (Smallanthus sonchifolius). Antibacterial activity of leaf extract by disk diffusion method with Bacillus subtilis and Escherichia coli respectively showed 13.3 and 13.75mm diameters of clear zone. There was no significant difference between the stems and leaves. The minimum inhibitory concentration of leaves' heating and agitation extraction showed a restrain of strain at 1mg/ml, but the stems and root extract did not appear. Yacon is a functional antibacterial material, and methanol extraction is more effective than water. This research was to investigate the growth stage of collection has the most effective antibacterial effects. It has collected yacon's leaves from June to October, which is an appropriate time for collection right before reaping. Yacon leaf has antibacterial effects on Bacillus subtilis, Escherichia coli, Enterococcus faecium, and Salmonella enteritifis. There were no significant differences by the growth stage of collection. Leaves collected in July are high in phenol which helps in sulfating activity works well considering the high scavenging capability of DPPH. Leaves collected in September are high in total flavonoid.

Optimization of Cultivation Conditions on Effective Seedlings of Veronica rotunda var. subintegra (Nakai) T.Yamaz. (산꼬리풀의 효과적인 육묘를 위한 재배조건 최적화)

  • Lee, Sang In;Yeon, Soo Ho;Cho, Ju Sung;Jeong, Mi Jin;Lee, Cheol Hee
    • Journal of Bio-Environment Control
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    • v.29 no.2
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    • pp.181-188
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    • 2020
  • This study was conducted to identify the optimal conditions for seedling growth in Veronica rotunda var. subintegra (Nakai) T.Yamaz. which a endemic plant and can be development as ornamental plants. We sowed V. rotunda var. subintegra (Nakai) T.Yamaz. seeds, and exposed the seedling was different treatment conditions. We varied soil type and fertilizer concentration, shading ratio, additional fertilizer concentration, pretreatment light exposure and collection time of seeds. We found that seedling growth was good in horticultural substrate (with no additional fertilizer), but mixed soils supplemented with fertilizer inhibited growth, regardless of the fertilizer concentration. In the 55% shading treatment, seedling growth was greater than in the non-shading treatment. High concentration addition of fertilizer (Hyponex) promoted plant growth, in terms of both plant length and fresh weight. Exposure of seeds to a red light-source prior to germination had a greater effect on seedling growth than exposure to other light sources. Seedlings exhibited better growth when grown from seed collected in 2018, rather than 2017.

Hypoxis aurea Lour. (Hypoxidaceae): a Rare Species from Jeju Island which is Rediscovered Seventy Years after its First Collection in Korea

  • Kim, Chan-Soo;Koh, Jung-Goon;Moon, Myong-Ok;Kim, Soo-Young
    • Korean Journal of Plant Resources
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    • v.21 no.3
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    • pp.226-229
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    • 2008
  • We described and illustrated a rare species in Korea, Hypoxis aurea Lour. (Hypoxidaceae) which was rediscovered about 70 years after its first collection from Jeju island in Korea. The members of the family Hypoxidaceae R. Br. are distinguished from the plants of Amaryllidaceae J. St-Hill. by having grass-like leaves, an invisible stem which is modified into a corm or a rhizome, trimerous, and radially symmetric flowers with an inferior ovary developing into a capsule on scapes. Hypoxis aurea Lour. is readily distinguishable from Curculigo orchinoides Gsertn. in Japan by beakless ovary and capsular fruit. The number of somatic chromosome is 2n=54.

SPSF : Smart Plant Safety Framework based on Reliable-Secure USN (차세대 USN기반의 스마트 플랜트안전 프레임워크 개발)

  • Jung, Ji-Eun;Song, Byung-Hun;Lee, Hyung-Su
    • Journal of The Institute of Information and Telecommunication Facilities Engineering
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    • v.9 no.3
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    • pp.102-106
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    • 2010
  • Recently process industries from oil and gas procedures and mining companies to manufactures of chemicals, foods, and beverages has been exploring the USN (Ubiquitous Sensor Networks) technology to improve safety of production processes. However, to apply the USN technology in the large-scale plant industry, reliability and security issues are not fully addressed yet, and the absence of the industrial sensor networking standard causes a compatibility problem with legacy equipment and systems. Although this situation, process industry such as energy plants are looking for the secure wireless plant solution to provide detailed, accurate safety monitoring from previously hard-reach, unaccordable area. In this paper, SPSF (Smart Plant Safety Framework based on Reliable-Secure USN) is suggested to fulfill the requirements of high-risk industrial environments for highly secure, reliable data collection and plant monitoring that is resistant to interference. The SPSF consists of three main layers: 1) Smart Safety Sensing Layer, 2) Smart Safety Network Layers, 3) Plant Network System Layer.

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