• Title/Summary/Keyword: Plant Species Identification

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Identification of Orchid Mycorrhizal Fungi Isolated from Five Species of Terrestrial Orchids in Korea (한국에 자생하는 5종의 지생란에서 분리한 난균근균의 동정)

  • Youm, Jae-Young;Han, Han-Kyeol;Chung, Jae-Min;Cho, Yong-Chan;Lee, Byung-Chun;Eom, Ahn-Heum
    • The Korean Journal of Mycology
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    • v.40 no.3
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    • pp.132-135
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    • 2012
  • This study was performed to isolate and identify the orchid mycorrhizal fungi (OMF) from roots of five species of terrestrial orchids in Korea; Cymbidium goeringii, Spiranthes sinensis, Calanthe discolor, Bletilla striata, Pogonia minor. DNA was extracted from isolated OMF and ITS region was amplified using primers, ITS1-OF and ITS4-OF. Four species of OMF belong to Tulasnellaceae and Sebacinaceae were identified; Tulasnella calospora, Tulasnella irregularis, Tulasnella sp., Sebacina vermifera.

Complete Genome Sequences of Crepidiastrum denticulatum (Asteraceae)

  • Jung, Joonhyung;Hyun, Jongyoung;Do, Hoang Dang Khoa;Kim, Joo-Hwan
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.37-37
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    • 2018
  • The genus Crepidiastrum (Asteraceae), containing ca. 20 species, is mainly distributed in Asia. Crepidiastrum denticulatum, an edible plant that commonly call "e-go-deulppae-gi" in Korean, distributes in Korea, Japan, and China. The complete chloroplast (cp) genome sequences of C. denticulatum was characterized from MiSeq2000 (Illumina Co.) pair-end sequencing data. The cp genome of C. denticulatum has a total sequence length of 152,689 bp and show a typical quadripartite structure. It consists of the large single copy (LSC: 84,022 bp), small single copy (SSC: 18,519 bp), separated by a pair of inverted repeats (IRs: 25,074 bp) and contains 110 unique genes and 18 genes duplicated in the IR regions. Our comparative analysis identified three cpDNA regions (matK, rbcL, and psbA-trnH) from three Crepidiastrum species, which may be useful for molecular identification of each species, and providing a guideline for its clear confirming about dried medical herb.

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Notes on the Aloe Vera Aphid, Aloephagus myersi Essig (Hemiptera: Aphididae) on Non-native Aloe Plants in Korea (우리나라 비자생 알로에식물의 알로에진딧물 (노린재목, 진딧물과)에 대한 보고)

  • Song, Jeong-Heub;Suh, Soo-Jung
    • Korean journal of applied entomology
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    • v.53 no.3
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    • pp.317-319
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    • 2014
  • The aloe vera aphid, Aloephagus myersi Essig, was collected on imported Aloe vera (L.) Burm.f. (Xanthorrhoeaceae) grown in greenhouses in Korea. This species is native to tropical Africa and probably was introduced into greenhouses via the aloe plant trade. This introduction reinforces the need to focus attention on the eradication from collected localities, and for constant surveillance and detection at ports of entry to prevent introduction and establishment of new pests in the Korean environment. In this paper, additional information for the aloe vera aphid is provided with diagnoses and photographs along with host plant and distribution data for accurate species identification.

Genetic Variation of Rice Populations Estimated Using nrDNA ITS Region Sequence

  • Wang, Dong;Hong, Soon-Kwan
    • Korean Journal of Plant Resources
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    • v.27 no.3
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    • pp.249-255
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    • 2014
  • The rice belonging to Oryza sativa is not only has significant economic importance, for it is the major source of nutrition for about 3 billion all around the world. But also plays a vital role as a model organism, because it has a number of advantages to be a model plant, such as efficient transformation system and small genome size. Many methods and techniques have been conducted to attempt to distinguish different Oryza sativa species, such as amplified fragment length polymorphism (AFLP), random amplified polymorphic DNA (RAPD), simple sequence repeat (SSR) and so on. However, studies using sequence analysis of internal transcribed spacer (ITS), a region of ribosomal RNA has not been reported until now. This study was undertaken with an aim to understand the phylogenetic relationships among sixteen isolates of Oryza sativa collected from abroad and fifteen isolates collected from Korea, using ribosomal RNA (rRNA) internal transcribed spacer (ITS) sequences to compare the phylogeny relationships among different Oryza sativa species. The size variation obtained among sequenced nuclear ribosomal DNA (nrDNA) ITS region ranged from 515bp to 1000bp. The highest interspecific genetic distance (GD) was found between Sfejare 45 (FR12) and Anapuruna (FR15). Taebong isolate showed the least dissimilarity of the ITS region sequence with other thirty isolates. This consequence will help us further understanding molecular diversification in intra-species population and their phylogenetic analysis.

Actual distribution and present status of a threatened aquatic plant, Sagittaria aginashi (Alismataceae), in Korea

  • Shiga, Takashi;Tsubota, Kazumasa;Choi, Hyeok Jae
    • Korean Journal of Plant Taxonomy
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    • v.47 no.3
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    • pp.180-188
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    • 2017
  • We evaluated the actual distribution, present status, and number of remnant individuals of Korean Sagittaria aginashi (Alismataceae) based on herbarium specimens and field surveys. We also reidentified Korean S. aginashi by analyzing sequences of the internal transcribed spacer (ITS) region. We found nine specimens of S. aginashi in herbaria from four localities housed at the Osaka Museum of Natural History (OSA) and at the Korea National Herbarium (KH). During our field research, we could not confirm the current statuses of two collection localities (Taereung and Ansan-si), though this was not the case for Ulsan-si. In addition, we found two new localities in Ulsan-si and Yangsan-si. These three remnant populations are distributed in wetlands in mountainous areas (elev. 480-740 m). With regard to the number of flowering and immature individuals in the three localities, there were <50 and <2,000, respectively, in total. ITS sequences demonstrated that the sequences of Korean and Japanese S. aginashi are uniform and distinct from other Sagittaria species. These results indicate that S. aginashi is a rare and threatened species in Korea. It should be listed as an endangered species on the Korean Red List and requires urgent protection by conservation programs, including the extensive surveys of other possible natural habitats.

The Hypersensitive Response. A Cell Death during Disease Resistance

  • Park, Jeong-Mee
    • The Plant Pathology Journal
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    • v.21 no.2
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    • pp.99-101
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    • 2005
  • Host cell death occurs during many, but not all, interactions between plants and the pathogens that infect them. This cell death can be associated with disease resistance or susceptibility, depending on the nature of the pathogen. The most well-known cell death response in plants is the hypersensitive response (HR) associated with a resistance response. HR is commonly regulated by direct or indirect interactions between avirulence proteins from pathogen and resistance proteins from plant and it can be the result of multiple signaling pathways. Ion fluxes and the generation of reactive oxygen species commonly precede cell death, but a direct involvement of the latter seems to vary with the plant-pathogen combination. Exciting advances have been made in the identification of cellular protective components and cell death suppressors that might operate in HR. In this review, recent progress in the mechanisms by which plant programmed cell death (PCD) occurs during disease resistance will be discussed.

Metabolic classification of herb plants by NMR-based metabolomics

  • Kim, Hee-Eun;Choi, Ye Hun;Choi, Kwang-Ho;Park, Ji Su;Kim, Hyeon Su;Jeon, Jun Hyeok;Heu, Min Soo;Shin, Dong-Seon;Lee, Joon-Hwa
    • Journal of the Korean Magnetic Resonance Society
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    • v.16 no.2
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    • pp.91-102
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    • 2012
  • Metabolomics is the systematic identification and quantification of all metabolites in an organism or biological sample. NMR has been used as a major application tool in plant metabolomics such as quality control, chemotaxonomy, and analysis of genetically modified plants. Herbal medicines are the important therapeutics and are used to manage common diseases such as cold, inflammation, pain, heart diseases, liver cirrhosis, diabetes and central nerve system diseases. Herb plants include various kinds of species such as geranium, mint, and thyme and so on and contain different kinds of metabolites. We performed NMR-based metabolomics study on the seven different species of herb plants using $^1H$ NMR experiments and OPLS-DA to understand the correlation between the classification of herb plants and their metabolite contents. This study showed clear metabolic discrimination among various herb plants. This metabolmics study found several diagnostic NMR signals which are able to be used as bio-markers for identification of the specific herb plants among various species. Clear metabolic discrimination of herb plants suggests three chemotaxonomic groups of herb species.

A taxonomic study of Korean Juncus based on the anatomical characters (한국산 골풀속의 해부학적 형질에 의한 분류학적 연구)

  • Jang, Chang-Seok;Oh, Byoung-Un
    • Korean Journal of Plant Taxonomy
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    • v.46 no.4
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    • pp.392-404
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    • 2016
  • The anatomical characters of the 19 taxa of Korean Juncus were reviewed in order to examine their significance in taxonomy. Cross-section analyses were conducted on the stem, leaf, and capsule. Descriptions, line drawings, tables, and a species key of Korean Juncus are provided for species identification based on anatomical characters. The results of this study showed that the arrangements of the vascular bundle, fiber, and air-space structure in the stems as well as the arrangements of the vascular bundle and parenchyma in the leaves and the development of placenta in capsules are the key characters to be used when classifying each species of Juncus. It was revealed that the studied anatomical features were more useful for identification at the subgenera and section levels than at the species level. Therefore, these anatomical characters used in conjunction with external morphological features will make a valuable contribution to establishing a natural classification system for Juncus.

Taxonomic identity of Dioscorea coreana (Prain & Burkill) R. Kunth

  • JEONG, Dae-Hui;KIM, Jae-Young;JO, Hyeong-Jun;PARK, Hong-Woo;LEE, Kang-Hyup;JI, Seong-Jin;CHUNG, Gyu-Young
    • Korean Journal of Plant Taxonomy
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    • v.50 no.2
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    • pp.232-238
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    • 2020
  • The identity of Dioscorea coreana (Prain & Burkill) R. Kunth is recognized during the re-identification process of Korean Dioscorea specimens. Given the relatively few pieces of information, including few descriptions and research papers, this species has been misidentified as D. tokoro, which has a similar leaf shape, but D. coreana is distinguished from D. tokoro by the absence of a pedicel in the male flower, the green color of the tepal, and the shapes of the fruit and seed. Thus, detailed descriptions, illustrations, and photographs of D. coreana and a key to the Korean Dioscorea, including this species, are presented.

Five Species of Syrphidae (Insecta: Diptera) Newly Recorded in Korea

  • Suk, Sang-Wook;Choi, Deuk-Soo;Han, Ho-Yeon
    • Animal Systematics, Evolution and Diversity
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    • v.31 no.4
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    • pp.257-265
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    • 2015
  • In Korean Peninsula, a total of 175 syrphid species are currently known. As a result of ongoing systematic study of the family Syrphidae, we have discovered the following five species for the first time in the Korean Peninsula: Mallota rossica Portschinsky, 1877, M. shatalkini Mutin, 1999, Sphiximorpha rachmaninovi (Violovitsh, 1981), Volucella bivitta Huo et al., 2007, and V. inanoides Hervé-Bazin, 1923. Among these taxa, Sphiximorpha Rondani, 1850, is the genus recorded for the first time in Korea. In total, three subfamilies, 16 tribes, 70 genera, and 180 species are now officially recognized for the Korean syrphid fauna. In order to facilitate their identification, we here provide specific diagnoses and color photographs for the species listed.