• Bulletin of the Korean Chemical Society
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• 제10권5호
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• pp.479-480
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• 1989

• Journal of Microbiology and Biotechnology
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• 제24권12호
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• pp.1644-1653
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• 2014

Wuyiencin is produced by Streptomyces ahygroscopicus var. wuyiensis CK-15 and is widely used as an antifungal agent in agriculture. Analysis of wuyiencin biosynthetic gene clusters reveals wysR, a member of the LAL-family of transcriptional regulatory genes. WysR consists of an N-terminal PAS domain and a LuxR family C-terminal helix-turn-helix motif. However, the roles of wysR in wuyiencin biosynthesis are largely unknown. In this study, we showed that inactivation of wysR resulted in the complete loss of wuyiencin production, which could be restored by complementation with a single copy of wysR. Furthermore, we successfully increased wuyiencin production to a significantly higher level by overexpression of wysR in S. wuyiensis CK-15. Quantitative real-time RT-PCR analysis showed that WysR regulates wuyiencin biosynthesis by modulating other putative regulatory genes. Thus, WysR was identified as an activator of wuyiencin biosynthesis, and overexpression of wysR gene proved to be an effective strategy for improving wuyiencin production.

• Nuclear Engineering and Technology
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• 제24권3호
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• pp.285-296
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• 1992

가압경수로형 원전을 위한 운전조정기의 개념 설계를 수행하였다. 운전조정기는 디지탈 감시 제한 계통으로서, 원전의 주요 운전 인자들을 감지하여 운전 제한치를 벗어나지 않도록 조정한다. 이는 운전 유연성을 향상시켜서 원전이 전력망 요구에 효과적으로 대처할 수 있도록 한다. 운전 허용 범위를 넘어서는 전력망 요구에 대해서 이를 제한하거나 제어 체계를 변경함으로써 이용률에 영향을 주지 않으며 운전을 수행할 수 있도록 한다. 이 개념은 1000 MWe급 가압경수로인 영광 3,4호기의 거동 모의를 통하여 평가되었다. 그 모의 계산 결과 운전 조정기가 원전의 운전 유연성 향상을 위하여 유용하게 사용될 수 있음을 보여 주었다.

• Journal of Plant Biotechnology
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• 제34권4호
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• pp.293-298
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• 2007

Robinia pseudoacacia var. umbraculifera, commonly called umbrella black locust were regenerated after co-cultivation of internode segments with Agrobacterium tumefaciens which included yeast cadmium factor 1 (YCF 1) gene. The tolerance to cadmium and lead for plants can be increased by the YCF1 gene expression. Moreover, the recent studies have shown that YCF1 gene transgenic plants increase the accumulation of cadmium and lead into plant vacuoles. The effect of plant growth regulator such as 2,4-dichlorophenoxyacetic acid (2,4-D), ${\alpha}$-naphthaleneacetic acid (NAA), 6-benzyladenine (BA), and thidiazuron (TDZ) were studied to evaluate the propagation of plants through internode explants. The efficient induction of multiple adventitious shoots and callus were observed on a medium supplemented with 0.1 mg/L TDZ + 0.2 mg/L BA. To induce shoot elongation and rooting, regenerated shoots were transferred into basal MS medium without any plant growth regulator. Successful Agrobacterium tumefaciens mediated transformation was obtained by 20 min vacuum-infiltration with $50{\mu}M$ acetosyringone on the optimal multiple shoot induction medium with 30 mg/L hygromycin and 300 mg/L cefotaxime. To confirm the integration and expression of transgene, Polymerase Chain Reaction (PCR) and Reverse Transcriptase PCR (RT-PCR) were performed with specific primers. The frequency of transformation was approximately 18.94%. This study can be used to genetic engineering of phytoremediator.

• 한국환경농학회지
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• 제34권2호
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• pp.111-119
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• 2015

BACKGROUND: Ethychlozate (ECZ) is a plant growth regulator of synthetic auxin for agricultural commodities (ACs). Accurate and sensitive method to determine ECZ in diverse ACs on global official purpose is required to legal residue regulation. As the current official method is confined to the limited type of crops with poor validation, this study was conducted to improve and extend the ECZ method using high-performance liquid chromatography (HPLC) in all the registered crops with method verification. METHODS AND RESULTS: ECZ and its acidic metabolite (ECZA) were both extracted from acidified samples with acetone and briefly purified by dichloromethane partition. ECZ was hydrolyzed to form ECZA and the combined ECZA was finally purified by ion-associated partition including hexane-washing. The instrumental quantitation was performed using HPLC/ FLD under ion-suppression of ECZA with no interference by sample co-extractives. The average recoveries of intra- and inter-day experiment ranged from 82.0 to 105.2% and 81.7 to 102.8%, respectively. The repeatability and reproducibility for intra- and inter-day measurements expressed as a relative standard deviation was less than 8.7% and 7.4%, respectively. CONCLUSION: Established analytical method for ECZ residue in ACs was applicable to the nation-wide pesticide residues monitoring program with the acceptable level of sensitivity, repeatability and reproducibility.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.164-164
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• 2022

The soybean (Glycine max(L.) Merrill), an important food crop in the world, is popular because of its high quality protein and oil content. Soybeans as a food have long been known for their beneficial effects on health and are well-recognized globally. Isoflavones, significant soybean secondary metabolic products, may be crucial in avoiding some cancers and lowering the risk of cardiovascular disorders. This study investigates the correlation between plant growth regulator and the effect on the isoflavone levels in soybean leaves. The study was carried out in the green-house of the southern crop department in miryang. Soybeans(Seonpung) were cultivated in 1/2000 of the Wagner pot. Ethephon(500, 1000, 2000 ppm) and ABA(100, 200, 400 ppm) were used as plant growth regulators, and they were each treated on R2, R5, and R7 stage. After treatment, leaves were sampled three times at intervals of 5 days, and the content of 6 isoflavones and coumestrol was analyzed. Soybean isoflavones were analyzed using Ultra Performance Liquid Chromatography (Acquity UPLC H-Class system, Waters). The isoflavones content showed an overall highly in the R5 stage, and the level was similar to that of no treatment in the R2 and R7 stage. The difference between the growth regulators was found to be higher than that of ethephon when ABA was treated. The coumestrol content was confirmed to be high in the order of R7, R5, and R2 on the treatment time, and it was found that the content increased as the treatment time was delayed. In the treatment with the growth regulator, the coumestrol content tended to be higher when ethephon was treated than ABA.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2008년도 제49회 학술심포지움 및 국제학술대회
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• pp.72.2-72.2
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• 2008

• 한국토양비료학회지
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• 제42권별호
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• pp.153-177
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• 2009

• 한국자원식물학회지
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• 제2권1호
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• pp.235-241
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• 1989

The study was conducted to determine the Ms orthogonaL modia and the concentration of plant growth regulator for seed matura-tion and growth of rhizome from Cymbidium goeringii Germination waswell in dark condition, but the growth of rhizome was better un-der dark than under light condition in MS orthoTonal . Sucrose con-centration( 3 %) gave better results than higher ones(6%), andthe use of NAA(0.1 PPm) effect significant difference of seed ge-rmination .But the growth of rhizome was best in medium Containingsucrose concentration(3%) Ippm NAA and 1 PPm BA.

• 한국약용작물학회지
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• 제12권4호
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• pp.285-288
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• 2004

꾸지뽕나무의 대량번식을 위한 상토와 생장조절제 처리에 따른 삽목 번식에 관한 시험을 수행한 바, 삽목상토는 미풍화토에서 양호하여 숙지삽과 녹지삽에서 각각 56.9%, 62.3%의 발근율을 보였고, 생장조절제는 IBA 100 ppm처리에서 발근이 양호하여 숙지삽에서 57.9%, 녹지삽에서는 89.2%의 발근율을 보였다.