• Horticultural Science & Technology
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• v.16 no.2
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• pp.244-246
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• 1998

This study was carried out to investigate the effects of plant growth regulators on the growth and development of pot 'Pinto Rose' and 'Pinto Scarlet' geraniums. Plant height was increased by $GA_3$ treatments in both 'Pinto Rose' and 'Pinto Scarlet' geranium. However, the number of branches was not affected by $GA_3$ and daminozide treatments. The combined treatments of $GA_3$ and daminozide delayed the days to first flowering in 'Pinto Scarlet' geranium. Also, in 'Pinto Rose', $GA_3$ or the combination of $GA_3$ and daminozide treatments delayed the first flowering time. 'Pinto Scarlet' geranium was more sensitive to growth regulator treatments than 'Pinto Rose'. The number of flower stalks and branches were increased by uniconazole in 'Pinto Scarlet', while the flower stalk length and canopy diameter were severely decreased by uniconazole treatments in both cultivars. The days to first flowering tended to be delayed by all growth retardant treatments in geranium.

• Journal of The Korean Society of Grassland and Forage Science
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• v.39 no.3
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• pp.153-157
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• 2019

Salicylic acid (SA) is an essential plant growth regulator that functions as a signaling molecule in plants. The purpose of this study was to clarify how the exogenous application of SA counteracts aluminum stress-induced growth and biomass yield reduction in alfalfa exposed to aluminum (Al) stress. Two-week-old alfalfa seedlings were exposed to a combination of $AlCl_3$ ($0{\mu}M$, $50{\mu}M$ and $100{\mu}M$, respectively) and SA (0.1 mM) for 72 hours. We observed, Al stress-induced plant growth inhibition and forage yield reduction are Al stress-dependent manner. A significant reduction of plant height (42.0-52.9%), leaf relative water content (13.0-21.4%), root length (35.4-48.7%), shoot fresh weight (31.2-25.9%), root fresh weight (15.4-23.3%), shoot dry weight (12.7-22.2%), roots dry weight (47.3-53.5%), were observed in alfalfa. In contrast, SA alleviated the Al-stress and enhanced growth and biomass yield in alfalfa. This study provides useful information concerning the role of SA that counteracts aluminum stress-induced growth and yield reduction in alfalfa.

• Development and Reproduction
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• v.26 no.1
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• pp.23-36
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• 2022

Pharyngeal pouches, a series of outgrowths of the pharyngeal endoderm, are a key epithelial structure governing facial skeleton development in vertebrates. Pouch formation is achieved through collective cell migration and rearrangement of pouch-forming cells controlled by actin cytoskeleton dynamics. While essential transcription factors and signaling molecules have been identified in pouch formation, regulators of actin cytoskeleton dynamics have not been reported yet in any vertebrates. Cofilin1-like (Cfl1l) is a fish-specific member of the Actin-depolymerizing factor (ADF)/Cofilin family, a critical regulator of actin cytoskeleton dynamics in eukaryotic cells. Here, we report the expression and function of cfl1l in pouch development in zebrafish. We first showed that fish cfl1l might be an ortholog of vertebrate adf, based on phylogenetic analysis of vertebrate adf and cfl genes. During pouch formation, cfl1l was expressed sequentially in the developing pouches but not in the posterior cell mass in which future pouch-forming cells are present. However, pouches, as well as facial cartilages whose development is dependent upon pouch formation, were unaffected by loss-of-function mutations in cfl1l. Although it could not be completely ruled out a possibility of a genetic redundancy of Cfl1l with other Cfls, our results suggest that the cfl1l expression in the developing pouches might be dispensable for regulating actin cytoskeleton dynamics in pouch-forming cells.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.64-64
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• 2023

콩은 중요한 식량 작물로 단백질, 기름 및 이차대사산물인 이소플라본을 다량으로 함유하고 있다. 이소플라본은 폴리페놀의 일종으로 일부 암을 예방하며 심혈관 질환을 완화하는 데에도 도움을 준다고 알려져 있다. 콩에 인위적으로 식물호르몬인 ABA를 처리할 때 이차대사산물인 kaempferol의 함량이 증가한다고 보고 되었다. 따라서 이 연구는 식물 생장조정제 처리가 콩잎의 이소플라본 함량에 미치는 영향을 알아보기 위해 수행되었다. 연구는 밀양 남부작물부 유리온실에서 수행되었으며, 콩(품종:선풍)은 와그너포트 1/2000에 1주 2본 파종하여 재배하였다. Ethephone(500, 1000, 2000 ppm)과 ABA(100, 200, 400 ppm)를 사용하였으며 각각 R2, R5, R7기에 처리하였다. 처리 후 잎을 5일 간격으로 3회 채취하여 이소플라본 6종과 쿠메스트롤의 함량을 분석하였다. 초고성능 액체 크로마토그래피(Acquity UPLC H-Class system, Waters)를 사용하여 분석하였다. 이소플라본 함량은 R5 단계에서 전반적으로 높게 나타났으며, R2 및 R7 단계에서는 무처리와 유사한 수준을 보였다. ABA를 처리하였을 때 생장조정제 간의 차이가 에테폰보다 더 큰 것으로 나타났다. 쿠메스트롤 함량은 처리시간에 따라 R7, R5, R2 순으로 높은 것으로 확인되었으며, 처리시간이 지날수록 함량이 증가함을 알 수 있었다. 생장조정제 처리에 쿠메스트롤 함량은 ABA보다 에세폰 처리 시 더 높은 경향을 보였다.

• 한국균학회소식:학술대회논문집
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• 2015.05a
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• pp.15-15
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• 2015

Aspergillus fumigatus is a major cause of invasive aspergillosis (IA), a significant health issue worldwide with high mortality rates up to 95%. Our lab is interested in how A. fumigatus adapts to low oxygen conditions 'hypoxia', which is one of the important host microenvironments. A. fumigatus SrbA is a basic helix-loop-helix (bHLH) transcriptional regulator and belongs to sterol regulatory element binding protein (SREBP) family members. Loss of SrbA completely blocks growth in hypoxia and results in avirulence in murine models of IA suggesting an essential role of SrbA in hypoxia adaptation and virulence in A. fumigatus. We conducted chromatin immunoprecipitation sequencing (ChIP-seq) with A. fumigatus wild type using a SrbA specific antibody, and 97 genes were revealed as SrbA direct targets. One of the 'SrbA regulons' (AFUB_099590) was a putative bHLH transcriptional regulator whose sequence contained a characteristic tyrosine substitution in the basic portion of the bHLH domain of SREBPs. Therefore, we designated AFUB_099590 SrbB. Further characterization of SrbB demonstrated that SrbB is important for radial growth, biomass production, and biosynthesis of heme intermediates in hypoxia and virulence in A. fumigatus. A series of quantitative real time PCR showed that transcription of several SrbA regulons is coordinately regulated by two SREBPs, SrbA and SrbB in hypoxia. This suggests that SrbA and SrbB have both dependent and independent functions in regulation of genes responsible for hypoxia adaptation in A. fumigatus. Together, our data provide new insights into complicated roles of SREBPs in adaptation of host environments and virulence in pathogenic fungi.

• Korean Journal of Weed Science
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• v.4 no.1
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• pp.62-68
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• 1984

This experiment was conducted to find out the possibility for reducing herbicidal dose of the recommended herbicides in combining with the plant growth regulator "chlorflurenol" at the Yeongnam Crop Experiment Station, from 1981 to 1982. Chlorflurenol itself exhibited weed suppression effect against Monochoria raginalis Presl., Rotala indica Koehne, Cyperus difformis L., Lindernia procrembens Philcox, etc. and its effect increased as the concentrations of chlorflurenol increased from 100 to 1,000 ㎖/㏊. Combination of chlorflurenol with butachlor or thiobencarb, showing synergistic effect, reduced their recommended herbicidal rates by 50 to 75%, showing 90% of weed control rate, stic but combined treatment between chlorflurenol and bentazon showed an antagonistic effect since bentazon had poor control ability to Echinochloa crusgalli Beauv. An application of chlorflurenol showed no significant effect on rice growth, in terms of dry weight, but it resulted in the decrease in the ratio of leaf blade to other non-photosynthetic organ.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.31-35
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• 1999

Morphological characteristic during formation of tobacco crown gall tumor and genetic tumor, and their differential response to growth regulator were investigated in in vitro culture. Crown gall tumor was induced from tumor tissue transformed by infecting Agrobacterium tumefaciens C58. Genetic tumor was induced from tumor tissue which was induced spontaneously from reciprocal interspecific hybrids between Nicotiana glauca (2n=24) and Nicotiana langsdorffii (2n=18). Morphological characteristic of crown gall tumor, genetic tumor, and teratoma shoot was very similar, and they were actively proliferated on hormone-free medium. Typical tumor callus and teratoma shoot formed from crown gall tumor on the hormone-free medium. On the contrary, tumor callus derived from genetic tumor formed as a crown gall tumor callus on the medium supplemented with 0.5 mg/L of 2,4-D, and lots of teratoma shoots without any root formed on the hormone-free medium. Root development from the teratoma shoots was hardly obtained on the medium with IAA, GA and active carbon. However, teratoma shoots with roots, as normal shoots, were initiated occasionally on the hormone-free medium. These shoots also formed new genetic tumor on the stem, which leaves formed lots of teratoma shoot on the hormone-free medium in in vitro culture.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.27-33
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• 2003

The optimal condition of in vitro regeneration and transformation were investigated for newly bred potato varieties in Korea. Leaf and internodal stem tissues of 'Chubak', 'Namsuh', 'Jasim', 'Jopung' and 'Jowon' were used to investigate the influence of growth regulator on regeneration efficiency The effect of phenolic compound acetosyringone on gene transformation efficiency was also investigated. Leaf tissue of 'Jowon' and internodal stem tissues of 'Jopung' were showed high regeneration efficiency on M5 medium supplemented with 0.1 mg/L GA₃, 2.0 mg/L Zeatin and 0.01 mg/L NAA. The other three potato cultivars were showed low regeneration efficiency less than 25%. The effect of acetosyringone on Agrobacterium-mediated gene transformation with leaf and internodal stem tissues were noticeable. By adding the 75 μM of acetosyringone during the Agrobacterium innoculation, the transformation efficiency was increased up to 1.5∼4.0 fold compare to non-treated control. In case of 'Jowon' the transformation efficiency was 87.9% in leaf tissue and 'Jopung' was 68.4% in internodal stem tissues.

• Korean Journal of Medicinal Crop Science
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• v.2 no.1
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• pp.1-6
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• 1994

These studies were carried out to investigated effect of cultural environment and of plant growth regulator on Macrocarpium officinale NAKAI. The flowering and fruitage were significantly different with location of cultivated area. Generally, suitable cultivation area was the basin of the mountains of 200-400m above the sea. The diurnal temperature change of GURYE was greater than KWANGSAN. The soil conditions of main cultivation area were sandy loam of slightly acid (pH 6.2-6.6). Dry fruit weight without seed of Macrocarpium officinale NAKAl was increased 18% in primary treatment of Gibberellin W.P (200g/10a) 15days before and secondary GA3 50ppm(6g /10a) 15 days after flowering compared with the control.

• Korean Journal of Plant Resources
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• v.26 no.2
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• pp.303-309
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• 2013

This study was performed to clarify the effect of medium compositions and plant growth regulators on the shoot, root formation and growth of 'Jarang' grape for mass propagation of virus-free plant. The formation and growth of shoot were considerably favorable in half-concentration of MS medium. However, the formation of adventitious root per explants (avg. 2.1) was effective in higher concentration (two times) of MS medium. For sucrose concentration, 1% for the shoot formation, 3% for the adventitious root formation and 1% for the growth were observed as yield significant results. With the addition of 0.05% of activated carbon, the shoot growth was improved, and it was effective for the adventitious root formation and growth as well. A pH of 6.8 in the medium was the most suitable for mass propagation; the results showed significant enhancement in the number of nodes and the length of the shoot, 3.9 and 1.3 cm, respectively. The shoot growth was the most vigorous in BA 1.0 mg/L due to the impact of the growth regulator on the mass propagation in it. Consequently, 16.9 shoots per explant were formed in NAA 1.0 mg/L so good results were obtained.