• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.4
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• pp.321-328
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• 2016

In this study, we investigated identification of anti-oxidative constituents from Ficus erecta var. sieboldii King (F. erecta) leaves. DPPH and $ABTS^+$ radical scavenging activities were screened for the ethanol extract and solvent fractions, and ethyl acetate fraction showed the most potent scavenging activities. Five constituents were isolated from the ethyl acetate fraction of F. erecta leaves; monoolein (1), oleic acid (2), lutein (3), afzelechin (4), catechin (5). The chemical structures of the isolated compounds were elucidated based on the spectroscopic data including NMR spectra, as well as comparison of the data to the literature values. As far as we know, all of the compounds 1 ~ 5 were isolated for the first time from this plant. Studies on DPPH and $ABTS^+$ radical scavenging activities were conducted for the isolated compounds. Among them, afzelechin (4) and catechin (5) showed strong DPPH and $ABTS^+$ radical scavenging activities, whose activities were comparable to a positive control vitamin C. Also, the content of catechin isolated from this plant was determined by HPLC and it was about 3.8 mg/g for the 70% ethanol extract and 20.8 mg/g for the ethyl acetate fraction. From these results, F. erecta leaves extract could be potentially applicable as anti-oxidant ingredients in cosmetic industries.

• Journal of the Korean Institute of Gas
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• v.22 no.3
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• pp.84-90
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• 2018

We researched the way to minimize the damage when the chlorine-leak accident take place in a purification plant. Since the level of risk based on the Off-site Risk Assessment(ORA) is a combination of proportional to the number of residents in the damaged area and frequency of accidents, we suggested the adequate conditions to reduce the number of residents in the damaged area by means of the operating temperature of a handling facility, installation of a emergency shut-off valve, and the analysis of the variation of the damaging range in accordance with the type of enclosure. The coverage of damage was calculated by the 'KORA(Korea Off-site Risk Assessment Supporting Tool) program. The research shows that the lower operating temperature gets, the more emergency shut-off valve being installed and the higher enclosure level of facility becomes, the extent of damage gets decreased. The decreasing rate of worst case was 17.6%, 71%, 34.5% respectively, the decreasing rate of alternative case was 31.6%, 69.0%, 34.8% respectively.

• Journal of the Korea Concrete Institute
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• v.26 no.2
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• pp.201-210
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• 2014

The present study proposes a phased model to assess the lifecycle $CO_2$ amount of concrete structures. The considered system boundary is from cradle to recycling, which includes constituent material, transportation, batching and mixing in ready-mixed concrete plant, use and demolition of structure, and crushing and recycling of demolished concrete. The $CO_2$ uptake of concrete by carbonation during lifetime (40 years) of a structure and the recycling life (20 years) after demolition is estimated using a simple approach generalized to predict the carbonation depth from the surfaces of concrete element and recycled aggregates. Based on the proposed phased model, a performance evaluation table is realized to straightforwardly examine the lifecycle $CO_2$ amount of concrete structures. The proposed model demonstrates that the contribution of ordinary portland cement (OPC) to lifecycle $CO_2$ emission of the concrete structure occupies approximately 85%. Furthermore, the $CO_2$ uptake is estimated to be approximately 15~18% of the lifecycle $CO_2$ emissions of concrete structures, which corresponds to be 19~22% of the emissions from OPC production. Overall, the proposed $CO_2$ performance table is expected to be practically useful as a guideline to determine the $CO_2$ emission or uptake at each phase of concrete structures.

• Horticultural Science & Technology
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• v.31 no.3
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• pp.388-392
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• 2013

A spray chrysanthemum cultivar 'Green Candy' was released by National Institute of Horticultural & Herbal Science, Rural Development Administration, in 2009. The cross between 'Yoko Ono', a spray chrysanthemum cultivar with light yellowish-green double type, and 'Green Bird', a spray chrysanthemum cultivar with vigorous and green double type, was made in Suwon in 2006. After the evaluation of the characteristics under shade culture in summer and retarding culture in spring and consecutive selection from 2007 to 2009, 'Green Candy' was selected finality. The natural flowering time of 'Green Candy' is October 28th, and it is possible to flower all year-round by shade and light culture. It has double flower type with green petals. The growth of plant is very vigorous and the color of the petals is stable when the variety is cultivated under high temperature conditions in summer season. The diameter of flower is 4.1 cm. The numbers of flower per stem and petals per flower are 12 and 217, respectively. The days for flowering under the short day treatment are about 58 and its vase life is 18.1 days in autumn season. 'Green Candy' was applied as No. 2010-212 on March 5, 2010 for variety protection and the plant variety protection rights have been registered as No. 3633 on August 2, 2011 at the Korea Seed and Variety Service.

• Research in Plant Disease
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• v.16 no.3
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• pp.224-231
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• 2010

Xanthomonas oryzae pv. oryzae, causal agent of bacterial leaf blight (BLB) of rice, had been collected and identified using Biolog and fatty acid analysis. Epidemics of BLB had been occurred all the times at several rice cultivating areas in Korea in 1999-2004. Most X. oryzae pv. oryzae isolated in 1999 and 2002 belonged to Korean race K1, but more than 50% of the pathogen isolated in 2003 belonged to Korean race K3. Especially, most pathogens isolated in Jeonnam and Joenbuk provinces belonged to Korean race K3. Inoculation test of near isogenic lines (NIL) of rice carrying single resistance genes against BLB showed that many isolates belonging to Korean race 1 reacted differently to diverse resistant monogenic lines of rice. Southern blot analysis also showed that the bacterial pathogens belonged to the same race had different numbers of avirulence genes. This results suggested that each Korean race type may respond to many resistance genes of rice. All the K3 races isolated in Jeonnam and Joenbuk provinces were able to cause disease on Xa3 monogenic lines of rice. Since most rice cultivars cultivated in Jeonnam and Jeonbuk were carrying Xa3 resistance genes, the bacterial pathogens isolated in Jeonnam and Jeonbuk were likely to develop to adapt to Xa3 resistance gene. Together with avirulence gene patterns of the bacterial isolates and the results of disease reaction of monogenic lines of rice to them, Korean X. oryzae pv. oryzae was classified into 19 pathotypes. This newly classified pathotypes should help the breeding of new resistance rice cultivars in Korea.

• Research in Plant Disease
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• v.16 no.3
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• pp.254-258
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• 2010

Melon necrotic spot virus (MNSV) is a very destructive disease to melon (Cucumis melo) plants. A MNSV was isolated from melon leaf showing necrotic spot symptoms at the plastic house in Naju, Korea in 2009. The isolate, designated as MNSV-Me, was identified and characterized by biological responses on several host plants, immuno captured RT-PCR and partial nucleotide sequencings of the genome. To evaluate MNSV-Me resistance in melon, thirty-five melon cultivars were mechanically inoculated on the cotyledon of the seedlings with the virus. MNSV-Me produced necrotic spots on the inoculated leaves of the all melon cultivars tested. Twenty-five cultivars were susceptible to the virus and they showed systemic necrotic spots on the leaves and/or necrosis longer than 3 cm in length on the stems within about forty days after inoculation. Five cultivars gave moderate resistance, no symptoms on the upper leaves but necrosis on the stem shorter than 3 cm in length. In an evaluation of MNSV-Me resistance in melon cultivars, 'Elstitan', 'Elsluxery', 'Betalichihage', 'Betalichi' and 'Womderfulhagae 1st' were found to have resistance by showing only faint necrosis on their stems.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.401-408
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• 2015

Horticultural traits and genetic relationship were evaluated for 83 melon (Cucumis melo L.) cultivars. Survey of a total of 36 characteristics for seedling, leaf, stem, flower, fruit, and seed and subsequent multiple analysis of variance (MANOVA) were conducted. Principal component analysis (PCA) showed that 8 principle components including fruit weight, fruit length, fruit diameter, cotyledon length, seed diameter, and seed length accounted for 76.3% of the total variance. Cluster analysis of the 83 melon cultivars using average linkage method resulted in 5 clusters at coefficient of 0.7. Cluster I consisted of cultivars with high values for fruit-related traits, Cluster II for soluble solid content, and Cluster V for high ripening rate. Genotyping of the 83 cultivars was conducted using 15 expressed-sequence tagged-simple sequence repeat (EST-SSR) from the Cucurbit Genomics Initiative (ICuGI) database. Analysis of genetic relatedness by UPGMA resulted in 6 clusters. Mantel test indicated that correlation between morphological and genetic distance was very low (r = -0.11).

• Journal of Plant Biotechnology
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• v.44 no.4
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• pp.394-400
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• 2017

This study investigated whether culture filtrates produced by Xylella fastidiosa can be used to determine the varietal susceptibility to Pierce's disease in grapevines (Vitis spp.) as a substitute for pathogen inoculation or field screening. A bioassay of grape leaves with culture filtrates from the pathogen showed that their phytotoxicities were active and host-selective. Ethyl acetate extracts from them also showed toxicities and host selectivity in both bunches of grapes and muscadine grapes. The sensitive range of plants to the culture filtrates and their ethyl acetate extracts was consistent with the host range of the Pierce's disease pathogen. Susceptible cultivars are sensitive to even highly diluted culture filtrates, while resistant cultivars were not affected even at their original culture filtrates. Susceptible cultivars were more sensitive to the undiluted culture filtrate than were highly diluted culture filtrates, and the younger leaves were the most sensitive to the culture filtrates in grapes. Although some European grape cultivars showed moderately susceptibility in this study, the determination of varietal resistance to Pierce's disease by the treatment of culture filtrates of pathogens could provide valuable information for the preliminary selection of genetic resources and seedlings from hybridization in a disease resistant grape breeding program.

• Korean Journal of Soil Science and Fertilizer
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• v.10 no.4
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• pp.219-224
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• 1978

To compare various methods for available soil P test, in terms of correlation between crop yield and amounts of available soil P, a pot experiment was conducted with soybean as test plant using four upland soils differing in phosphorus status. The results are summarized as following. 1. There was highly significant correlation between grain yield and amount of P uptake observed at the floral differentiation stage. Highly significant correlations were also observed between grain yield and available soil P of different methods, and between the amount of P uptake and available soil P. The consistency of correlations between soil P and plant parameters was Olsen${\geq}$Lancaster P>Bray No.1 P${\geq}$Bray No.2 P>Truog P. 2. The magnitute of available soil P values of different methods was in the order of Bray No.2>Lancaster P${\geq}$Bray No.1 P>Olsen P>Truog P. 3. The amounts of available P measured by various methods showed significant correlation with the amounts of Al-P, Fe-P and Ca-P. 4. As conclusion, Olsen-P, Lancaster-P, and Bray-P are reliable information for evaluating soil P status. However, Olesen-P seemed to be better than the others.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1061-1061
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• 2001

The scope of precision agriculture is to reach the put up cultivation goals by adjusting inputs as precise as possible after what is required by the soil and crop potentials, on a high spatial resolution. Consequently, precision agriculture is also often called site specific agriculture. Regulation of field inputs “on the run” has been made possible by the GPS (Geographical Position System)-technology, which gives the farmer his exact real time positioning in the field. The general goal with precision agriculture is to apply inputs where they best fill their purpose. Thus, resources could be saved, and nutrient losses as well as the impact on the environment could be minimized without lowering total yields or putting product quality at risk. As already indicated the technology exists to regulate the input based on beforehand decisions. However, the real challenge is to provide a reliable basis for decision-making. To support high spatial resolution, extensive sampling and analysis is required for many soil and plant characteristics. The potential of the NIR-technology to provide rapid, low cost analyses with a minimum of sample preparation for a multitude of characteristics therefore constitutes a far to irresistible opportunity to be un-scrutinized. In our work we have concentrated on soil-analysis. The instrument we have used is a Bran Lubbe InfraAlyzer 500 (1300-2500 nm). Clay- and organic matter-contents are soil constituents with major implications for most properties and processes in the soil system. For these constituents we had a 3000-sample material provided. High performance models for the agricultural areas in Sweden have been constructed for clay-content, but a rather large reference material is required, probably due to the large variability of Swedish soils. By subdividing Sweden into six areas the total performance was improved. Unfortunately organic matter was not as easy to get at. Reliable models for larger areas could not be constructed. However, through keeping the mineral fraction of the soil at minimal variation good performance could be achieved locally. The influence of a highly variable mineral fraction is probably one of the reasons for the contradictory results found in the literature regarding organic matter content. Tentative studies have also been performed to elucidate the potential performance in contexts with direct operational implications: lime requirement and prediction of plant uptake of soil nitrogen. In both cases there is no definite reference method, but there are numerous indirect, or indicator, methods suggested. In our study, field experiments where used as references and NIR was compared with methods normally used in Sweden. The NIR-models performed equally or slightly better as the standard methods in both situations. However, whether this is good enough is open for evaluation.