• 한국산림과학회지
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• 제107권3호
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• pp.245-257
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• 2018

본 연구는 전남 홍도를 대상으로 하여 산림식생구조를 밝힐 목적으로 2017년 6월부터 8월까지 총 41개소의 방형구를 설치하여 식생조사를 수행하였고, 식물사회학적 방법으로 식생유형을 분류하고 분류된 식생단위별 상대중요치와 종다양도를 산출하였다. 산림식생유형분류 결과 최상위수준에서 구실잣밤나무군락군으로 분류되었으며, 군락단위에서는 참식나무군락, 소사나무군락으로 분류되었다. 참식나무군락은 천선과나무군(식생단위 1), 큰천남성군(식생단위 2)으로 세분되었고, 소사나무군락은 쇠물푸레나무군(식생단위 3)과 소사나무전형군(식생단위 4)으로 세분되어, 총 4개의 식생단위가 분류되었고, 1개의 군락군, 2개의 군락, 4개의 군으로 분류되었다. 식생단위별 평균상대중요치 분석 결과 식생단위 1, 2, 4는 구실잣밤나무의 평균상대중요치가 가장 높았고 식생단위 3은 소사나무가 가장 높았다. 종다양도 분석 결과 식생단위 3이 가장 높은 종다양도를 나타내었다. 따라서 홍도 일대는 4개의 식생단위와 7개의 종군단위를 바탕으로 한 군집생태학적 접근의 관리방안이 필요하며, 또한 상록활엽수림으로 진행되어가는 천이연구가 이루어져야 할 것으로 사료되었다.

• 한국습지학회지
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• 제25권4호
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• pp.237-247
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• 2023

논은 다양한 생태계 서비스를 제공할 뿐만 아니라 생물다양성 보전에 중요한 서식지이며, 최근 생태학적 가치와 중요성이 점점 강조되고 있다. 따라서 본 연구는 영농 방식이 서로 다른 관행 논(Conventional Paddy field, CP)과 유기 재배 논(Organic Paddy field, OP)을 대상으로 토양 종자은행의 특성을 파악하고 토양 환경 요인과의 상관관계를 분석하였다. 연구결과 관행 논의 식생은 단순하여 종자은행의 식물 다양성이 낮은 반면에, 유기 논의 식생은 상대적으로 다양하여 종자은행의 식물 다양성이 높게 나타났다. 또한 토양 환경 특성도 유기 논 (K, Ca, Mg, Na, Fe, Mn, B↓) 과 관행 논(K, Ca, Mg, Na, Fe, Mn, B↑)의 명확한 차이를 보였다. 이러한 결과는 벼 재배 방식에 대한 영농 활동의 차이가 식생과 토양 종자은행의 구조와 다양성에 영향을 주었고 또한 토양 환경 요인에도 직간접적으로 영향을 준 것으로 판단된다. 본 연구결과는 농업생태계 생물다양성 보전과 생태계 복원 그리고 생태적 가치를 평가하기 위한 기초자료로 활용될 수 있다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.43-43
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• 2018

Mutation breeding by radiation is useful for improving various crop species. Up to now, a total of 170 soybean mutant varieties have been released in the world, which is the second most registered varieties after rice. Despite the economic importance of soybean, there have been no TRAP marker system studies on genetic relationships between/among mutant lines. To develop a strategy of Mutant Diversity Pool (MDP) conservation, a study on the genetic diversity of 210 soybean mutant lines (8 cultivars and 202 mutants) was performed through a TRAP analysis. Sixteen primer combinations amplified a total of 551 fragments. The highest (84.00%) and lowest (32.35%) polymorphism levels were obtained with primers MIR157B + Ga5 and B14G14B + Ga3, respectively. The mean PIC values 0.15 varied among the primer combination ranging from 0.07 in B14G14B + Sal2 to 0.23 in MIR157B + Sa4. Phylogenetic, principal component analysis (PCA) and structure analysis indicated that the 210 lines belong to four groups based on the 16 combination TRAP markers. AMOVA showed 21.0% and 79.0% variations among and within the population, respectively. Overall, the genetic similarity of each cultivar and its mutants were higher than within other mutant populations. Our results suggest that the TRAP marker system may be useful for assessing the genetic diversity among soybean mutants and help to improve our knowledge of soybean mutation breeding.

• 한국자원식물학회지
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• 제29권3호
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• pp.322-330
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• 2016

Understanding the genetic variation among landrace collections is important for crop improvement and utilization of valuable genetic resources. The present study was carried out to analyse the genetic diversity and associated population structure of 621 foxtail millet accessions of Korean landraces using 22 EST-SSR markers. A total of 121 alleles were detected from all accessions with an average of 5.5 alleles per microsatellite locus. The average values of gene diversity, polymorphism information content, and expected heterozygosity were 0.518, 0.594, and 0.034, respectively. Following the unweighted neighbor-joining method with arithmetic mean based clustering using binary data of polymorphic markers, the genotypes were grouped into 3 clusters, and population structure analysis also separated into 3 populations. Principal coordinate analysis (PCoA) explained a variation of 13.88% and 10.99% by first and second coordinates, respectively. However, in PCoA analysis, clear population-level clusters could not be found. This pattern of distribution might be the result of gene flow via germplasm exchanges in nearby regions. The results indicate that these Korean landraces of foxtail millet exhibit a moderate level of diversity. This study demonstrated that molecular marker strategies could contribute to a better understanding of the genetic structure in foxtail millet germplasm, and provides potentially useful information for developing conservation and breeding strategies.

• Journal of Ginseng Research
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• 제40권4호
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• pp.315-324
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• 2016

Background: Biocontrol agents are regarded as promising and environmental friendly approaches as agrochemicals for phytodiseases that cause serious environmental and health problems. Trichoderma species have been widely used in suppression of soil-borne pathogens. In this study, an endophytic fungus, Trichoderma gamsii YIM PH30019, from healthy Panax notoginseng root was investigated for its biocontrol potential. Methods: In vitro detached healthy roots, and pot and field experiments were used to investigate the pathogenicity and biocontrol efficacy of T. gamsii YIM PH30019 to the host plant. The antagonistic mechanisms against test phytopathogens were analyzed using dual culture, scanning electron microscopy, and volatile organic compounds (VOCs). Tolerance to chemical fertilizers was also tested in a series of concentrations. Results: The results indicated that T. gamsii YIM PH30019 was nonpathogenic to the host, presented appreciable biocontrol efficacy, and could tolerate chemical fertilizer concentrations of up to 20%. T. gamsii YIM PH30019 displayed antagonistic activities against the pathogenic fungi of P. notoginseng via production of VOCs. On the basis of gas chromatography-mass spectrometry, VOCs were identified as dimethyl disulfide, dibenzofuran, methanethiol, ketones, etc., which are effective ingredients for antagonistic activity. T. gamsii YIM PH30019 was able to improve the seedlings' emergence and protect P. notoginseng plants from soil-borne disease in the continuous cropping field tests. Conclusion: The results suggest that the endophytic fungus T. gamsii YIM PH30019 may have a good potential as a biological control agent against notoginseng phytodiseases and can provide a clue to further illuminate the interactions between Trichoderma and phytopathogens.

• Plant Breeding and Biotechnology
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• 제5권3호
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• pp.243-251
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• 2017

Rhus chinensis is a shrub widely distributed in Asia. It has been used for traditional medicine and ecological restoration. Here, we report the complete chloroplast genome sequence of two R. chinensis genotypes collected from China and Korea. The assembled chloroplast genome of Chinese R. chinensis is 149,094 bp long, consisting of a large single copy (97,246 bp), a small single copy (18,644 bp) and a pair of inverted repeats (16,602 bp). Gene annotation revealed 77 protein coding genes, 30 tRNA genes, and 4 rRNA genes. A phylogenomic analysis of the chloroplast genomes with 11 known complete chloroplast genomes clarified the relationship of R. chinensis with the other plant species in the Sapindales order. A comparative chloroplast genome analysis identified 170 SNPs and 85 InDels at intra-species level of R. chinensis between Chinese and Korean collections. Based on the sequence diversity between Korea and Chinese R. chinensis plants, we developed three DNA markers useful for genetic diversity and authentication system. The chloroplast genome information obtained in this study will contribute to enriching genetic resources and conservation of endemic Rhus species.

• 한국환경복원기술학회지
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• 제17권3호
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• pp.35-51
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• 2014

A phytosociological vegetation survey was conducted in July to September 2011 in order to examine the vegetation community structure in Mt. Ohseo area. It was aimed to provide basic data for the effective vegetation conservation by analyzing the importance, species diversity and community similarity of the forest community in Mt. Ohseo for each layer, followed by the classification of the actual forest vegetation. According to the cluster analysis, the community type of Mt. Ohseo was classified into a total of 4 vegetation communities: Pinus densiflora community, Cornus controversa-Quercus serrata community, Miscanthus sinensis community, and Quercus mongolica community; the vegetation type 4 showed the lowest species diversity index of 0.5236, and vegetation type-2 showed the highest species diversity index of 0.6606. The community similarity between Quercus mongolica community and Pinus densiflora community showed the highest 0.679, and the community similarity between Quercus serrata community and Pinus densiflora community and between Quercus serrata community and Quercus mongolica community showed the levels of 0.5, respectively.

• Journal of Forest and Environmental Science
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• 제38권1호
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• pp.12-20
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• 2022

Pongamia (Pongamia pinnata L.) as a source of non-edible oil, is potential tree species for biodiesel production. For several reasons, both technical and economical, the potential of P. pinnata is far from being realized. The exploitation of genetic diversity for crop improvement has been the major driving force for the exploration and ex situ/in situ conservation of plant genetic resources. However, P. pinnata improvement for high oil and seed production is not achieved because of unsystematic way of tree improvement. Performance of P. pinnata planted by Karnataka Forest Department was assessed based on yield potential by collecting 157 clones out of 264 clones established by Karnataka Forest Department research wing under different research circles/ranges. It was evident that the all the seed and pod traits were significantly different. Further, selection of superior germplasm based on oil and pod/seed parameters was achieved by application of Mahalanobis statistics and Tocher's technique. On the basis of D² values for all possible 253 pairs of populations the 157 genotypes were grouped into 28 clusters. The clustering pattern showed that geographical diversity is not necessarily related to genetic diversity. Cluster means indicated a wide range of variation for all the pod and seed traits. The best cluster having total oil content of more than 34.9% with 100 seed weight of above 125 g viz. Cluster I, II, III, IX, XV, XIX, XXI, XXIII, XXVI and XXVII were selected for clonal propagation.

• 한국습지학회지
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• 제22권2호
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• pp.100-105
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• 2020

수변 및 육상 식생의 경계에 서식하는 쥐방울덩굴(Aristolochia contorta)은 국내 취약종인 꼬리명주나비(Sericinus montela) 유충의 유일한 기주식물이라는 점에서 높은 보전가치를 지닌다. 개체군의 장기적인 유지에 있어서 유전적 다양성은 반드시 고려되어야 하며, 이를 위하여 기존 개체군의 유전적 다양성을 파악하는 과정이 선행되어야 한다. 쥐방울덩굴 개체군이 장기적으로 유지되고 있는 네 서식처의 개체군을 대상으로 개체들의 잎을 채집하여 DNA를 추출하였으며, 5개의 무작위 프라이머를 이용한 RAPD-PCR을 수행하여 각 개체군의 유전적 다양성을 비교하고 개체군 간의 유연관계를 파악하였다. 개체군 내 유전적 다양성은 4개 개체군 중 가평 개체군(GP)이 가장 높았으나, 전반적인 유전적 다양성은 다른 종에 비해 낮은 편으로 나타났다(h: 0.0607 ~ 0.1491; I: 0.0819 ~ 0.1759). 또한 가평 개체군의 경우 지리적 거리와 무관하게 다른 개체군들과의 유전적 거리가 큰 편으로 나타났다. 이는 파편화된 서식지와 더불어 낮은 유성생식 비율에서 기인한 것으로 추정된다. 쥐방울덩굴 개체군의 보전을 위해서는 개체군 혼합 식재와 적절한 차광이나 물리적 지지와 같이 쥐방울덩굴의 유성생식 을 촉진하는 환경이 적극적으로 고려되어야 할 것이다.

• 한국환경생태학회지
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• 제16권2호
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• pp.195-216
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• 2002

울릉도의 식물상은 총 109과 355속 526종 2아종 89변종 10품종 총 627종류(Taxa)로 파악되었다. 현재까지 보고된 울릉도 식물상은 총 122과 465속 762종 5아종 178변종 21품종 9교잡종 총 975종류로 정리되었다. 울릉도에 분포하는 식물의 특성을 밝히기 위하여 한반도, 울릉도 및 일본열도 간 식물상을 비교한 결과, 울릉도의 식물은 한반도와 더 유사한 관계를 보이는 것으로 밝혀졌다. 울릉도에 자생하는 특산식물을 포함하여 희귀 및 멸종위기식물의 서식처는 보다 체계적인 관리가 필요하다. 또한, 관광객의 증가로 귀화식물이 점점 늘어나고 있는 추세이므로 외래종에 대한 관리가 필요할 것으로 판단된다. 울릉도의 특정 생태계나 특산식물을 포함한 희귀 및 멸종위기식물의 서식처를 보전하기 위하여 생태계 보전지역 이상의 수준에서 보전지역으로 지정하여, 점차 파괴되어 가는 울릉도의 자연생태계를 보호해야 할 것이다.