• Proceedings of the PSK Conference
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• 2003.10b
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• pp.157.1-157.1
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• 2003

This study was carried out to evaluate the cytotoxicity and anti-inflammatory effects of Resina Pini on cultured human gingival fibloblasts. We carried out a study of cytotoxic effects of Resina Pini on cultured cells by MTT assay. Various treatments on Resina Pini reduced its toxicity on cultured cells in order of natural Resina Pini, water extracted mixture of Resina Pini and Ramus Mori Albae and recrystalized Resina Pini. However, Resina Pini showed harmless levels of cytotoxicity to cultured human gingival fibroblast. (omitted)

• YAKHAK HOEJI
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• v.46 no.5
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• pp.348-351
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• 2002

In this report, anti-inflammatory, analgesic and cytotoxic effects of the water extracts of Pini Resina, which has been as an additive to oral hygienic products together with sodium chloride in community, were investigated. The water extracts of Pini Resina, pretreated Pini Resina and Ramus Mori Albae-added Pini Resina all showed relatively low cytotoxicity. All samples showed concentration-dependent increase in electron-donating capacity to DPPH, especially, Ramus Mori Albae-added Pini Resina was the highest in the extent. Arachidonic acid release from the cell membrane was significantly inhibited by the presence of the samples above, among those, Ramus Mori Albae-added Pini Resina was the most effective in the inhibitory action of the release.

• Natural Product Sciences
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• v.11 no.1
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• pp.27-32
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• 2005

The purpose of this study is to evaluate the inhibitory effects of Resina Pini against Streptococcus mutans (S. mutans) that is one of the major causes of dental caries and oral diseases. Topically applied Resina Pini (RP) would be incorporated in saliva and thus the factor associated with water solubility should be considered. In this paper, therefore, effects of various treatment for RP and activities of water extracts from unprocessed and processed RP were compared. The crude RP (RP1) and the recrystallized RP (RP2) in ethanol solution showed strong antimicrobial activities (d.>15mm) against S. mutans. All RP samples exhibited considerable inhibitory effect against glucosyltransferase produced by S. mutans $(IC_{50}=91.2\;to\;276.2\;{\mu}g/ml)$. The very considerable increase in cellular permeability of S. mutans was observed with RP1, RP2 and their water extracts. These results suggest that RP1 and RP2 may be a potential source for pharmaceutical products used for prevention and/or treatment of dental caries and periodontal disease.

• Natural Product Sciences
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• v.13 no.4
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• pp.347-354
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• 2007

In this study, we investigated the potential of Resina Pini (RP) for anti-inflammatory and analgesic agents to treat inflammatory diseases such as gingivitis and periodontitis. Crude RP (RP1), recrystallized RP (RP2), and Ramus Mori Albae-treated RP (RP3), plus their respective water extracts (RP1-WE, RP2-WE and RP3-WE) were prepared for in vitro and in vivo tests. We couldn't find any signs of heavy metals pollution in all the RP samples. RP2-WE exhibited the highest viability of human gingival fibroblasts (HGF) and the strongest scavenging activity on superoxide anion. RP1, RP2 and RP3, RP2 showed potent scavenging activity on DPPH free radical. RP2-WE displayed a stronger inhibition on hyaluronidase (HAase) activity and RP3 also displayed potent HAase inhibition. RP2-WE, RP3-WE, RP3 and RP2 were reduced admirably the production of $PGE_2$ in HGF. In addition, RP2-WE and RP3-WE exhibited potent inhibitory activities on arachidonic acid-induced ear edema in mouse. Moreover, RP-2 prevented completely acetic acid-induced writhing by 100.0% and RP1, RP3, RP1-WE and RP2-WE also exhibited excellent protective activities against writhing. While aminopyrine, the positive control, showed 76.9% analgesic effect at the same dose. Taken together, these results suggest that recrystallized aqueous extract of Resina Pini could be a promising drug for the treatment of periodontal diseases.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.42 no.3
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• pp.203-209
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• 2016

Recently, increased attention has been directed toward medicinal extracts and their active ingredients as potential new drug candidates for androgenic alopecia. Resina Pini (RP), a resinous exudation obtained from Pinus sp. (Pinaceae), has been used as a traditional medicine for the treatment of infection, pain related to dental caries, and periodontal disease. Previously, we suggested that RP and its main constituent, abietic acid (abieta-7,13-dien-18-oic acid; AA), may play important roles against androgenic alopecia as $5{\alpha}$-reductase inhibitors. However, to date, there is no evidence that AA has hair growth-promoting effects in vivo. In this study, we found that 10 ~ 300 mg/kg RP and 3 ~ 30 mg/kg AA significantly promoted hair growth in a C3H/HeN mouse model of alopecia. To our knowledge, this is the first report of the hair growth-promoting effects of RP and AA in vivo. From these results, RP and its main constituent AA can promote hair growth in mouse by inhibiting $5{\alpha}$-reductase activity and may be effective alternative therapies for androgenic alopecia.

• Proceedings of the PSK Conference
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• 2001.10a
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• pp.205.3-206
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• 2001

• Journal of Haehwa Medicine
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• v.9 no.1
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• pp.691-710
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• 2000

We came to the conclusion after considering all the information from many kinds of book on herb medicine which is treatment method of "quickening blood and transforming stasis" for external wound. 1. Angelicae sinensis Radix and Angelicae Gigantis Radix are account for 16%(57times) of monarch drug and ministerial drug in all. It means that effect of "quickening blood and transforming stasis" of Angelicae sinensis Radix and Angelicae Gigantis Radix are more excellent than any other herb medicine. 2. In the case of the monarch drug, Angelicae sinensis Radix and Rhizoma Rhei are used at most, treatment method is divided into "expel stasis" and "quickening blood and transforming stasis". 3. In the case of the monarch drug, there are Dolomitum, Eupolyphaga, Dracinis Resina, Crinis Carbonisatus, Acronychiae Ligum, Pini Ramulus etc which have strong treatment effect and pecunliar sort. 4. In the case of the ministerial drug, there are Dracinis Resina, Brassica Rapa, Pyritunm, Liquidambaris Resina etc which are unusual sort. 5. In the order of frequency in use of monarch drug and ministerial drug in nature, it is hot, mean, cold drug. In the order of frequency in use of monarch drug and ministerial drug in flavors, it is sharp, pungent, sweat.

• Korean Journal of Food Science and Technology
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• v.34 no.4
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• pp.652-660
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• 2002

To develop natural food preservatives for extending the shelf-life of jeotkal (salted and fermented seafood), antimicrobial substances were extracted from 32 types of medicinal herbs and edible plants using 95% ethanol. Among the extracts, Glycyrrhizae radix, Curcumae domestica, Galla rhois, and Resina pini showed relatively high inhibitory effects on the growth of the microorganisms isolated from the deteriorated jeotkal. We selected and tested the extract from Recina pini as a natural jeotkal preservative. This ethanol extract was purified partially by adding equal quantity of water, through which 77% of insoluble materials were removed as impurities. In manufacturing modified jeotkal using squid, sucrose and starch syrup were substituted with sorbitol, $glucono-{\delta}-lactone$ was added instead of vitamin C and lactic acid, and sterilized hot pepper was used instead of natural one. The shelf-life of modified jeotkal was prolonged by 4 days compared with the control jeotkal when stored at $20^{\circ}C$, while that of modified jeotkal containing 1.0% partially purified Recina pini extract was prolonged by 6 days compared to the control. The same tests were conducted for the changran (stomach and intestine of Alaska pollack) jeotkal preservation. The shelf-life of the control jeotkal was 24 days, whereas the modified jeotkal and the Resina pini extract-containing modified jeotkal maintained their qualities without changes in microbial and chemical characteristics for 90 days at $20^{\circ}C$ storage.