• Journal of Korean Society of Forest Science
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• v.50 no.1
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• pp.72-73
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• 1980

The spermagonial state of Cronartium ribicola J. C. Fisher appeared on the discolored bark of Korean pine (Pinus koraiensis Sieb. & Zucc.) from late August through middle of October in Korea. A large number of ellipsoid or ovoid spermatia were contained in the spermagonial exudates that appeared on the bark of infected trees. In the spring (April-May) following the appearance of spermagonia, aecia broke through that part of the bark which had been occupied by spermagonia the year before.

• Journal of the Korean Wood Science and Technology
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• v.47 no.4
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• pp.498-508
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• 2019

This study was carried out to investigate the potential of Korean red pine (Pinus densiflora) inner bark extracts as an antidiabetic agent. The ethyl acetate soluble fraction of the bark extracts was chromatographed on a Sephadex LH-20 column to yield five compounds, which structures were elucidated by NMR spectroscopy. The isolated compounds were (+)-catehin, (-)-epicatechin, taxifolin, taxifolin-3'-O-${\beta}$-D-(+)-glucose and $\tilde{n}$-courmaric acid. The antidiabetic activity of the different fractions, including the crude extracts and isolated compounds, was evaluated by ${\beta}$-cells insulin secretion and glucose uptake in skeletal muscle cells. The insulin secretion was 128% for taxifolin at $25{\mu}g/mL$. However, the other samples had no effect on this test. For the glucose uptake activity assay, $1{\mu}M$ insulin and 2 mM metformin were used as controls. Both the crude extract and taxifolin showed relatively low activity values, but the other samples yielded glucose uptake values over 260%. ${\rho}$-courmaric acid showed the highest uptake (270%). The results confirmed that Korean red pine extracts may be used as a hypoglycemic agent.

• Journal of the Korean Wood Science and Technology
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• v.47 no.1
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• pp.80-89
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• 2019

Pine barks are important biomass resources because they are utilised in the production of pine wood and rosins. However, no chemical study has been conducted on the hydrophilic status of pine barks in Indonesia. This aim of this study is to explore the hydrophilic extracts of the barks from six Pinus species (P. elliotii, P. caribeae, P. oocarpa, P. merkusii P. montezumae, and P. insularis). The hydrophilics of pine barks were analysed using gas chromatography-mass spectrometry. The presence of polyphenol contents in the ethanol extracts obtained from the barks of six Pinus species was determined using the tannin-formaldehyde method, Folin-Cioucalteu assay, and vanillin-HCl assay. The ethanol and hot water soluble extractives derived from inner barks were higher in quantity when compared to those derived from the outer bark samples. The polyphenol measurement showed that the highest value of total phenol content was derived from the outer bark of P. montezumae whereas those of the total phenol and tannin- formaldehyde contents were derived from the inner and outer barks of P. oocarpa. GC-MS analysis revealed that nitrogenous compounds are dominant constituents in the inner and outer barks of the six species, followed by sugars and monophenolics, respectively.

• Fashion & Textile Research Journal
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• v.15 no.1
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• pp.138-146
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• 2013

Chemical compositions and biological functions of brown colorants extracted from pine bark(Pinus densiflora) have been studied. Dyeing test using multifiber fabrics with extracted colorants were preliminary carried out. Dyeing conditions and fastness tests of selected fabrics have been also studied. The brown colorants were produced 1.5% concentrations by solvent extraction from milled pine bark using methanol. The colorants were extracted with 80% methanol as best choice by a criteria of solid quantity and dyeability on fabrics. The chemical compositions were identified as mixtures of taxifolin epicatechin and procyanidin by LC/MS analysis. The brown colorants could be dyed not only natural fibers such as cotton, silk and wool but also synthetic fiber as nylon and semi-synthetic fiber as viscose rayon. Maximum K/S values was shown at 400 nm according to different fiber with color appearance of redish brown. Optimum pH and temperature of dyeing conditions was 4 and above $80^{\circ}C$, respectively. The brown colorants had a strong antioxidant activity compared to Butylated hydroxyanisole as standard and weak antimicrobial activity against E. coli. compared to kanamycin. Washing, rubbing, perspiration, dry cleaning and light fastness for cotton, nylon and silk dyed with the brown colorants were carried out by KS K method. Most of color fastness such as washing, rubbing, perspiration, and dry cleaning were represented as 4-5 grade. However, light fastness was reported as 2-3 grade. From this studies, brown colorants produced pine bark have a high potentials for natural dyeing on fabrics with antioxidant activity.

• Journal of Pharmaceutical Investigation
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• v.37 no.6
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• pp.359-364
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• 2007

Pine bark extract is well-known as a very powerful antioxidant, anti-inflammatory, and antibiotic material. French maritime pine bark extract ($Pycnogenol^{(R)}$) of Horphag Research has monopolized the world market over 30 years. Korean red pine bark extract ($Pinexol^{(R)}$) was first manufactured by the patent technology of NutraPharm in Korea in 2006. Feasibility of topical gel and patch formulations of Pinexol was systematically investigated by evaluating the skin absorption of catechin as a reference compound. In vitro hairless mouse skin absorption of catechin from gel formulation was higher than that from patches. However, significant amount of catechin was also deposited inside the skin from patch formulations, which were dependent on the types of pressure sensitive adhesives. Thus, it seems to be feasible to control the topical delivery of Pinexol by using both gel and patch formulations, and be necessary to conduct further systematic investigation.

• Journal of Korea Foresty Energy
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• v.21 no.2
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• pp.17-24
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• 2002

Activated carbons were prepared from pine bark by steam activation, and pore structures and specific surface areas were then investigated. Three different types of kilns were used for the activation. When the stationary-vertical-or stationary-horizontal-type kiln was used for the steam activation to prepare an activated carbon from the bark, it was not possible to produce activated carbon having high specific surface areas exceeding 1,000 $m^2／g$. Using bark powder improved the specific surface area, but it was still not high enough. When the rotary-horizontal-type kiln was used for the activation, the activated carbons prepared had high specific surface areas of more than 1,000$m^2／g$, which was similar to a commercial first-grade activated carbon. The activated carbon prepared by the rotary kiln had a wide distribution of pore size ranging from microporous to mesoporous.

• Journal of Microbiology and Biotechnology
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• v.28 no.5
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• pp.679-687
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• 2018

Korean red pine (Pinus densiflora) is one of the major Pinus species in Korea. Red pine bark is removed prior to the chipping process in the wood industry and discarded as waste. However, red pine bark contains a considerable amount of naturally occurring phenolics, including flavonoids, and therefore may have a variety of biological effects. In this study, we investigated if Korean red pine bark extract (KRPBE) could protect neuronal PC-12 cells from oxidative stress and inhibit cholinesterase activity. Analysis of reversed-phase high-performance liquid chromatography results revealed four phenolics in KRPBE: vanillin, protocatechuic acid, catechin, and taxifolin. The total phenolic and flavonoid contents of KRPBE were 397.9 mg gallic acid equivalents/g dry weight (DW) and 248.7 mg catechin equivalents/g DW, respectively. The antioxidant capacities of KRPBE measured using ABTS, DPPH, and ORAC assays were 697.3, 521.8, and 2,627.7 mg vitamin C equivalents/g DW, respectively. KRPBE and its identified phenolics protected against $H_2O_2$-induced oxidative cell death in a dose-dependent manner. Acetylcholinesterase and butyrylcholinesterase, which degrade the neurotransmitter acetylcholine to terminate neurotransmission in synaptic clefts, were inhibited by treatment with KRPBE and its identified phenolics. Taken together, these results suggest that KRPBE and its constituent antioxidative phenolics are potent neuroprotective agents that can maintain cell viability under oxidative stress and inhibit cholinesterase activity.

• Proceedings of the Korea Concrete Institute Conference
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• 1998.04b
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• pp.747-750
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• 1998

The application of spent liquor(BSL) obtained from alkaline sulfite-anthraquinone cooking of pine bark to cement mortar significantly improved the water-reducing ability and decreased the rate of cement hardening. However, the compressive and flexural strength of BSL addition to cement mortar were lower than that of PLAIN. The application of 0.2% antifoamer to BSL slightly decreased water-reducing ability, but remarkably improved the compressive and flexural strength of cement mortar. On the other hand, BSL decreased the rate of hardening of cement, which exhibited the protperties of the lignosulfonated based retarding water-reducing type. but the setting time of cement could be controlled by addition of Na2CO3 could be used to convert BSL to normal or accelerating concrete admixtures.

• Journal of Korean Society of Forest Science
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• v.94 no.5 s.162
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• pp.302-306
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• 2005

Litterfall and nutrient inputs were measured in even-aged coniferous plantations (a 31-year-old Pinus rigida and a 31-year-old Larix leptolepis) on a similar site condition in the Forest Practice Research Center, Gyeonggi Province. Litterfall was collected monthly from circular littertraps (collecting area: $0.50m^2$) for three years between April 1997 and February 2000. Average total annual litterfall was significantly higher for pine (5,802 kg/ha/yr) than for larch (4,562 kg/ha/yr) plantations. Needle litter in both plantations accounted for about 63% of total litterfall. Litterfall in the larch was distributed as follows: needle > other leaf > branch > miscellaneous > bark, while it was needle > miscellaneous > other leaf > branch > bark in the pine plantation. There was no temporal variation in needle litter, other leaf and bark during the 3 year study period. The concentrations of all nutrients (N, P, K, Ca, Mg) in needle litter were significantly higher in the larch than in the pine plantations. The annual nutrient concentration of needle litter in the larch varied among the years, whereas no year variation of needle litter was in the pine except for phosphorus (P). Nitrogen (N) and P inputs by needle litter were significantly higher for larch than for pine plantations established on a similar soil. The differences in N and P inputs were attributed to lower nutrient concentration in pine needle litter compared with larch needle litter, not to total needle litter mass. Annual inputs of nutrient in both plantations were not significantly different among years except for K of the larch although there was yearly different in needlefall mass and nutrient concentration during the 3-year observed period. The results indicate that the mechanisms of litterfall and nutrient inputs vary considerably between pine and larch plantations established on a similar site condition.

• Journal of the Korean Wood Science and Technology
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• v.27 no.2
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• pp.46-52
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• 1999

Alkaline sulfite-anthraquinone(AQ) cooking was examined for their effectiveness in delignification of pine (Pinus densiflora S. et Z.) bark. Better delignification was achieved with alkaline sulfite liquor compared to monosulfite liquor, but the degree of delignification was less than 90% indicating need for improvement in cooking method, Remarkable increase in the rate of cooking and delignification was obtained by the simple addition of AQ to the alkaline sulfite cooking, Selectivity of delignification was also improved by the addition of AQ, Consequently, 90% of delignification was achieved with the addition of 0.2% AQ to the alkaline sulfite cooking. The selectivity of delignification was slightly affected by cooking temperature and NaOH concentration during alkaline sulfite-AQ cooking of the bark. The selectivity of delignification was highly affected by the concentration of $Na_2SO_3$, and the optimum concentration of $Na_2SO_3$ for the high delignification was 30% as $Na_2O$.