• Biotechnology and Bioprocess Engineering:BBE
• /
• 제9권2호
• /
• pp.76-85
• /
• 2004

Biodevices composed of biomolecular layer have been developed in various fields such as medical diagnosis, pharmaceutical screening, electronic device, photonic device, environmental pollution detection device, and etc. The biomolecules such as protein, DNA and pigment, and cells have been used to construct the biodevices such as biomolecular diode, biostorage device, bioelectroluminescence device, protein chip, DNA chip, and cell chip. Substantial interest has focused upon thin film fabrication or the formation of biomaterials mono- or multi-layers on the solid surfaces to construct the biodevices. Based on the development of nanotechnology, nanoscale fabrication technology for biofilm has been emerged and applied to biodevices due to the various advantages such as high density immobilization and orientation control of immoblized biomolecules. This review described the nanoscale fabrication of biomolecular film and its application to bioelectronic devices and biochips.

• 대한화장품학회지
• /
• 제23권3호
• /
• pp.86-91
• /
• 1997

Some natural polyhydroxy aromatic compounds have inhibitory activity against tyrosinase, key enzyme for formation of melanin pigment. We examined the structure-activity relationship of the natural polyhydrowy aromatic compounds and synthesized a number of new derivetives through various methods. Skin lightening effects of these compounds were examined through inhibition of mushroom tyrosinase and inhibitory of melanogenesis on B-16 melanoma cells. These new compounds showed strong inhibitory activity against tyrosinase. Good lightening effects sue to inhibition of melanogenesis were observed from several resorcinol and pyrogallol derivatives. In toxicological tests such as skin primary irritation and sensitization, the above compounds were sufficiently safe for cosmetic use.

• 한국임상수의학회지
• /
• 제8권1호
• /
• pp.71-80
• /
• 1991

Clinical signs, serum chemical values and histological findings of hepatic tissue after partial and complete obstruction of common bile duct in Korean goats were investigated. Abnormal clinical signs were not observed in partial obstruction of common bile duct, but in complete obstruction clinical signs such as jaundice, urine color change, were observed. Serum total bilirubin, total cholesterol, aspartate aminotransferase, sorbitol dehydrogenase, gamma glutamyltranspeptidase, and total protein values increased on the 1-4th day and then gradually decreased to normal level in partial obstruction. However, they tend to increase persistently by the 24th day in complete obstruction of common bileduct. Histologic features of hepatic tissue in partial obstruction were not changed as compared with normal hepatic tissue. On the other hand, in complete obstruction of common bite duct there were moderate bile duct proliferation in a portal area, rupture of bile canaliculi, phagocytosis of bile pigment by Kupffer cells, periportal fibrosis, intrahepatic bile stasis and hepatic cell necrosis.

• Molecules and Cells
• /
• 제41권4호
• /
• pp.257-263
• /
• 2018

Vertebrate organ development is accompanied by demarcation of tissue compartments, which grow coordinately with their neighbors. Hence, perturbing the coordinative growth of neighboring tissue compartments frequently results in organ malformation. The growth of tissue compartments is regulated by multiple intercellular and intracellular signaling pathways, including the Hippo signaling pathway that limits the growth of various organs. In the optic neuroepithelial continuum, which is partitioned into the retina, retinal pigment epithelium (RPE) and ciliary margin (CM) during eye development, the Hippo signaling activity operates differentially, as it does in many tissues. In this review, we summarize recent studies that have explored the relationship between the Hippo signaling pathway and growth of optic neuroepithelial compartments. We will focus particularly on the roles of a tumor suppressor, neurofibromin 2 (NF2), whose expression is not only dependent on compartment-specific transcription factors, but is also subject to regulation by a Hippo-Yap feedback signaling circuit.

• Journal of Chest Surgery
• /
• 제35권9호
• /
• pp.697-700
• /
• 2002

50세 여자가 전흉부의 통증을 주소로 내원하였다. 흉부단층촬영에서 우상엽을 침범하고 있는 전종격동의 종괴가 발견되었으며, 수술로서 완전절제하였다. 조직검사에서 악성 흑색종을 진단할 수 있었고, 원발병소의 가능성이 있는 피부, 구강, 안저, 비강, 직장, 항문, 질 등을 각각의 전문의에게 검사를 받았으나 이상소견이 없었다. 원발병소가 자연적으로 소멸된 경우가 아니라면 종격동에 원발한 악성 흑색종이라고 생각된다. 종격동에 발생하는 악성 흑색종은 아주 드문 질환으로 세계적으로 8례만이 보고되고 있고, 한국에서는 아직 예가 없기에 보고하는 바이다.

• Journal of Yeungnam Medical Science
• /
• 제19권1호
• /
• pp.68-72
• /
• 2002

저자들은 신생아시기에서부터 담즙정체성을 나타낸 Dubin-Johnson 증후군 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• 대한의생명과학회지
• /
• 제13권4호
• /
• pp.313-317
• /
• 2007

Melanogenesis refers to the biosynthesis of melanin pigment in melanocytes. Melanogenesis is controlled by the intra- and extracellular environments. In the present study, to develop a new whitening agent, it was investigated the antioxidant activity and the inhibitory effect of Ailanthi Radicis Cortex extract on tyrosinase activity and on melanogenesis in the B16/F1 melanoma cells. The inhibition ratio of tyrosinase activity of ethylacetate fraction from Ailanthi Radicis Cortex was higher than that of arbutin. The ethylacetate fraction showed scavenging activities of 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and superoxide anion radicals in a dose dependent manner. The highest inhibitory activity of melanogenesis was also in ethylacetate fraction ($40.0{\pm}5%$ at the concentration of $400{\mu}g/ml$). This study demonstrates that the Ailanthi Radicis Cortex extract might be used to be a potential agent for skin whitening.

• 한국식물병리학회지
• /
• 제14권6호
• /
• pp.598-602
• /
• 1998

Twenty two rhizobacteria were isolated from the roots and rhizosphere of radish, carnation, potato and tomato. There isolates produced a fluorescent pigment in King's B medium and identified as Pseudomonas spp. These isolates colonized roots and rhizosphere of the host plants. In the study of cultural characteristics of the bacteria, the pH of the culture broth was changed from neutral (7.0) to alkali (8.8∼9.41) and the numbers of cells were increased from 106 to 108 after 40 hr of incubation in basal standard succinate medium. The salicylic acid production identified by pink color reaction were observed in 7 bacteria. Out of these 7 salicylic acid producing bacteria, only 2 strains of bacteria such as Pseudomonas fluorescens RS006, and Pseudomonas sp. EN401 were confirmed as salicylic acid producers by optical density measurement. Therefore, for screening of salicylic acid producing bacteria from the roots and rhizosphere, color reaction of the culture medium should be done in the first step, and then optical density measurement of culture extract should be made for the confirmation of salicylic acid production.

• Molecular & Cellular Toxicology
• /
• 제4권4호
• /
• pp.300-306
• /
• 2008

We investigated high light-induced alterations in antioxidant enzymes by exposing green vegetative cells of the alga Haematococcus pluvialis to excess irradiance to induce the production of astaxanthin, a carotenoid pigment. Total activity of catalase decreased approximately 70% after high light exposure, whereas glutathione peroxidase (GPX) activity was slightly enhanced. Total activity of superoxide dismutase and ascorbate peroxidase (APX) also slightly decreased. Overall, we did not observe dramatically elevated levels of antioxidant isozymes, although APXn, GPX2, and GPX3 isozyme increased slightly. ${H_2}{O_2}$ content increased about sixfold after high light exposure, demonstrating severe cellular oxidative stress, whereas lipid peroxidation was notably reduced. Concomitantly, astaxanthin accumulation increased about sevenfold. This result suggests that probably massively accumulated astaxanthin may be one of the antioxidant protector against high light stress.

• 자연과학논문집
• /
• 제19권1호
• /
• pp.27-35
• /
• 2008

The importance of microbial activity in the alteration and deterioration of stone and concrete walls has been frequently neglected. Organisms present on stone monuments can include photolithoautotrophs, such as algae, cyanobacteria, mosses, and higher plants. Because of their ability to survive repeated drying and rehydration cycles and high UV levels, the cyanobacteria are particularly important on exposed surfaces. The cyanobactria distributed on the surface of the stone cultural heritages in Gwanschoksa, Nonsan city were investigated. Chlorococcus sp. Aanabaena sp. Gloeocapsa sp Lyngbya sp. Stigomena sp. Synechocystis sp were identified. Haplaosiphon fontinalis and Stigonema turfaceum, which were not recoded is Korea, were also identified. Cells often have thick pigmented sheath in dry, sun-exposed environment and shorter filament, which can be different than that in aquatic systems. Special attention should be paid to production of an adequate DNA database in order to accelerate the rate at which information on the species present in biofilms become available.