• Asian-Australasian Journal of Animal Sciences
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• v.5 no.2
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• pp.285-294
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• 1992

Biochemical polymorphisms of sow's milk proteins, $\beta$-casein ($\beta$-CN), $\beta$-lactoglobulin ($\beta$-LG), post-lactoglobulin (post-LG), $\alpha$-lactalbumin ($\alpha$-LA) and X-protein, as genetic markers for major pig breeds (Landrace, Yorkshire, Duroc, Hampshire and cross bred) in Korea were determined by starch gel electrophoresis. Phenotype and gene frequencies at all marker loci were estimated and genetic differences among breed populations were analyzed. Three $\beta$-CN phenotypes (AA, AB and BB) controlled by two codominant alleles (${\beta}-CN^A$ and ${\beta}-CN^B$), four $\beta$-LG phenotypes (AA, AC, $AC^{\pm}$ and CC) controlled by two codominant alleles (${\beta}-LG^A$ and ${\beta}-LG^C$) and ten X-protein phenotypes (AA, BB, CC, DD, AB, AC, AD, BC, BD and CD) controlled by four codominant alleles ($X^A,\;X^B,\;X^C\;and\;X^D$) were identified. In addition, a genetically controlled polymorphism of post-LG was found for the first time in sow's milk protein. Three different phenotypes (AA, AB and BB) were designated $post-LG^A$ and $post-LG^B$. Of the five marker loci examined, $\alpha$-LA locus was observed to lack any individual variation in all breeds studied. All populations were in Hardy-Weinberg equilibrium for all loci. There were marked breed differences for phenotype and gene frequencies in the post-LG and X-protein marker loci. However, there were little differences between breeds in the gene frequencies at the $\beta$-CN and $\beta$-LG marker loci.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.6
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• pp.908-913
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• 1999

This experiment was conducted in three trials to evaluate optimal ambient temperature for a novel milk replacer feeding system designed for early-weaned pigs, compared to commercial dry diets fed within a conventional hot nursery. A total of 165 PIC genotype pigs were weaned at $13.89{\pm}0.7$ days of age and allotted to one of two dietary treatments in three trials based on weight and litter origin. Each trial consisted of pigs fed dry diets (DD) and pigs fed milk replacer (MR) which was offered in one of 3 different ambient temperatures. Pigs fed milk replacer were housed in a specialized nursery building in which one half of each pen contained an enclosed hover that was thermostatically maintained at $32^{\circ}C$ while the exterior ambient temperature (where milk was fed) was set at either 17 (trial 1), 24 (trial 2) or $32^{\circ}C$ (trial 3). Pigs fed dry diets with the conventional nursery were maintained at $30^{\circ}C$ for each trial. From d 21 to d 49, all pigs were fed DD within a standardized hot nursery environment. During the first week (d 14-21), pigs fed MR showed increased ADG from 214% to 228% over control pigs fed DD (p<0.001), regardless of ambient temperature. As ambient temperature was increased from 17 to 24 to $32^{\circ}C$, ADG of MR-fed pigs was increased by 214%, 220% and 228% over those of pigs fed DD, respectively. ADFIs of MR-fed pigs at $17^{\circ}C$, $24^{\circ}C$, and $32^{\circ}C$ compared with pigs fed DD were increased by 108%, 139% and 164% from d 14 to d 21, respectively. Fed efficiency (G/F) of MR-fed pigs at $17^{\circ}C$, $24^{\circ}C$, and $32^{\circ}C$ compared with pigs fed DD were 199%, 162% and 139% of those of pigs fed DD, respectively. As ambient temperature increased, diarrhea scores showed a slight tendency to increase. The advantage of MR feeding was greater when the ambient temperature was higher, but G/F was impaired with increased ambient temperature. We conclude that ambient temperature within the specialized nursery influenced behavior, MR feed intake, and probably piglet energy expenditure. There were no differences between MR-fed and DD-fed pigs for ADG, ADFI and G/F in the subsequent growth period (d 21 to d 49, p>0.05). Maximal advantage of MR feeding was obtained at the intermediate ($24^{\circ}C$) ambient temperature during the overall period (p<0.05). Results from this experiment indicate that a milk replacer feeding system utilized in the early postweaning period can maximize pig growth performance, and that ADG, ADFI and G/F were affected by different ambient temperatures within MR-fed pigs. The high or low temperatures could not support the maximal growth of pigs fed MR.

• Journal of Korean Medical classics
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• v.25 no.3
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• pp.39-49
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• 2012

Objective : "Sikryochanyo"written by Jeon Soonyi is the first Food-Therapy book at Korea. In "Sikryochanyo", there are many kinds of food-Therapy including prescriptions for thirst disease. Conception of hirst disease in traditional mendicine contains diabetes in some aspects. One of the Ingeative methods in diabetes limits taking protein but "Sikryochanyo" shows several prescriptions including protein. Method : We will try to find out cases of Food-therapy for thirst disease in "Sikryochanyo" and classify several prescriptions including protein. Result : In "Sikryochanyo", several prescriptions for thirst disease including protein were treated as materia medica widely such as stomach of cow and pig, intestine of pig, head of dear, rabbit, chicken, goose, pheasant, milk and lung of sheep. Conclusion : On several types of prescriptions for thirst disease including protein there are plenty of usage with its own effectiveness.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.2
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• pp.283-300
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• 2004

Currently commercial dry sows are housed in individual stalls and subject to restricted feeding. These sows often show stereotypic behaviours which increase their maintenance energy requirement. Group housing is desirable to improve animal welfare and public perception. However, under restricted feeding systems, group-housed dry sows are also aggressive. The feed intake of these sows is variable, depending on their social rank, which results in different milk production and variable piglet performance. These problems can be solved by ad libitum feeding systems, but the large capacity of intake by dry sows will not allow this feeding system to be practical as high feeding level during pregnancy can reduce reproduction performance of sows. Current research indicates that feeding high fibre diets to dry sows enables sows to be fed ad libitum, but the effect of dietary fibre on feed intake and nutrient utilisation is dependent on the quality of fibre sources. Most research has focused on sugar beet pulp, straw, lucerne meal and by-products, but there is a need to identify and evaluate some widely available and cheap fibre materials and feed grains for developing the best strategy to control nutrient intake of dry sows while feeding ad libitum.

• Korean Journal of Animal Reproduction
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• v.27 no.4
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• pp.317-324
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• 2003

The present study were performed to analysis the hematocrit and the red blood cells content into the blood plasma of the transgenic pigs harboring recombinent human erythropoietin gene (rhEPO). Mouse whey acidic protein (mWAP) linked to rhEPO gene was microinjected into pronuclei of porcine one-cell zygotes. After delivered of offspring, PCR analyses identified one mWAP-rhEPO transgenic founder offspring(F$_{0}$). The first generation of transgenic pig (F$_{0}$) harboring mWAP-hEPO appeared to be a male, and the second generation (F$_1$) pigs were made by natural mating of F$_{0}$ with domestic swine, and male and female transgenic pigs (F$_1$) were identified by PCR. The blood samples from transgenic and normal pigs were collected for 50 days during lactation and were counted the red blood cell (RBC) numbers and Hematocrit (HCT) content into the blood. The transgenic pigs expressing rhEPO in their blood gave rise to higher RBC numbers and HCT contents than control animals. rhEPO was secreted both in the blood and milk of genetically engineered pigs harboring rhEPO gene. Therefore, this study provides a model regarding the production of transgenic pig carrying hEPO transgene for biomedical research.earch.

• Asian-Australasian Journal of Animal Sciences
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• v.13 no.11
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• pp.1604-1608
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• 2000

Penicillin antibiotics such as penicillin G, ampicillin and amoxicillin have been widely used in the pig industry to control salmonellosis, bacterial pneumonia, and urinary tract infections. Extensive use of antibiotics in veterinary clinics has resulted in tissue residues and bacterial resistance. To prevent unwanted drug residues entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid prediction test for the detection of pigs with unacceptable tissue residues of penicillins. The recommended therapeutic doses of three penicillins, penillin G (withdrawal time, 7 days), ampicillin (withdrawal time, 7 days) and amoxicillin (withdrawal time, 14 days), were administered to three groups of 20 pigs each. Blood was sampled before drug administration and during the withdrawal period. The concentration of penicillins in plasma, determined by a semi-quantitative ELISA, were compared to that of internal standard, 4 ppb, which corresponded to the Maximum Residue Limit in milk. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in pig tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive, and larger than 1 negative. All 60 plasma samples from pigs were negative to three penicillins at pretreatment. Penicillin G could be detected in the plasma of the treated pigs until day 4 post-treatment and ampicillin until day 2, whereas amoxicillin could be detected until day 10 of its withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted to detect residues of penicillin antibiotics (penicillin G, ampicillin and amoxicillin) in live pigs.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.8 no.1
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• pp.156-166
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• 2007

Soybean meal was widely used as a protein source in pig feedstuff because it has a good amino acid balance compared with other vegetable sources. However, soybeans contain trypsin inhibitors and other antinutritional factors which can lead to lower the digestibility of amino acid, and consequently reduce the growth performance. Heat treatment of soybeans is helpful shown to decrease the antinutritional factors and elicit an improved growth performance. Additionally, microbial processe using(HP 100, HP 200 and HP 300), and non-protein constituent removal are suggested to improve the growth performance and nutrient digestibility. Inadequate heat treatment of soybeans gives no damage to adult pig, but it has been shown to decrease nutrient digestibility in young pig. So, soy protein concentrate (SPC) and Isolated soy protein(ISP) were more widely used for nursery pigs than growing and finishing pigs, since SPC and ISP have similar characteristics as milk product.

• Korean Journal of Veterinary Service
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• v.39 no.4
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• pp.231-237
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• 2016

The present study was conducted to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. During March to December 2015, a total of 1,160 lung samples out of 58 herds were collected randomly from pigs slaughtered in Jeonbuk province. In addition, 290 hilar lymph nodes from pig with pneumonic lung lesion (5 samples per herd) were screened for selected viral and bacterial pathogens. Gross lesions of lungs such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis and liver white spots were examined. The overall prevalence of SEP was 64.3% (746/1,160). In the analysis of seasonal prevalence, there was an increase of occurrence during the spring months (287/400, 71.8%) and decrease during the fall months (93/200, 46.5%) among the whole herds. The mean number of SEP score per pig was $1.20{\pm}1.28$. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 25.5% (296/1,160), 44.1% (512/1,160), 3.8% (44/1,160) and 17.6% (204/1,160), respectively. The most frequent region with lung lesion was diaphragmatic lobes (left 17.1%, right 17.3%). In the detection of viral pathogens by PCR, porcine circovirus type2 (PCV2) was positive in 86.9% (252/290), while porcine reproductive and respiratory syndrome virus (PRRSV) was not detected, In the case of bacterial pathogens, 50 microorganisms were isolated by PCR and/or microbiological test. The most frequently isolated bacteria was Streptococcus suis (20, 34.4%), followed by Pasteurella multocida (17, 29.3%), Streptococcus spp. (11, 3.4%), Actinobacillus pleuropneumoniae (2, 8.9%).

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.3
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• pp.421-427
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• 2012

The production of therapeutic proteins from transgenic animals is one of the most important successes of animal biotechnology. Milk is presently the most mature system for production of therapeutic proteins from a transgenic animal. Specifically, ${\beta}$-casein is a major component of cow, goat and sheep milk, and its promoter has been used to regulate the expression of transgenic genes in the mammary gland of transgenic animals. Here, we cloned the porcine ${\beta}$-casein gene and analyzed the transcriptional activity of the promoter and intron 1 region of the porcine ${\beta}$-casein gene. Sequence inspection of the 5'-flanking region revealed potential DNA elements including SRY, CdxA, AML-a, GATA-3, GATA-1 and C/EBP ${\beta}$. In addition, the first intron of the porcine ${\beta}$-casein gene contained the transcriptional enhancers Oct-1, SRY, YY1, C/EBP ${\beta}$, and AP-1, as well as the retroviral TATA box. We estimated the transcriptional activity for the 5'-proximal region with or without intron 1 of the porcine ${\beta}$-casein gene in HC11 cells stimulated with lactogenic hormones. High transcriptional activity was obtained for the 5'-proximal region with intron 1 of the porcine ${\beta}$-casein gene. The ${\beta}$-casein gene containing the mutant TATA box (CATAAAA) was also cloned from another individual pig. Promoter activity of the luciferase vector containing the mutant TATA box was weaker than the same vector containing the normal TATA box. Taken together, these findings suggest that the transcription of porcine ${\beta}$-casein gene is regulated by lactogenic hormone via intron 1 and promoter containing a mutant TATA box (CATAAAA) has poor porcine ${\beta}$-casein gene activity.

• Animal Bioscience
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• v.34 no.5
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• pp.833-843
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• 2021

Objective: Current study was conducted to determine the effect of postpartum prostaglandin F2α (PGF2α) administration on colostrum and milk yield, colostrum immunoglobulin G (IgG) and piglet growth performance. Methods: In total, 36 sows were included in the experiment. The sows were classified into two groups: i) control (n = 11) and ii) PGF2α (n = 25). Sows in the PGF2α group received 10 mg of PGF2α within an hour after farrowing. The body weight of piglets was measured at 0 and 24 h after birth to estimate colostrum consumption. Colostrum was collected at 1 and 24 h after farrowing to determine IgG concentrations. For milk yield study, the remaining sows in the PGF2α group (n = 23) were divided into two subgroups: i) single PGF2α (n = 12) and ii) multiple PGF2α (n = 11). In the multiple PGF2α, the sows received repeated doses of PGF2α at seven and 14 days postpartum. The piglets' body weight was measured at 0, 1, 5, and 20 days of age. The milk yield of the sows was calculated. Results: Colostrum yield of sows averaged 5.62±2.25 kg. Sows treated with PGF2α postpartum had a higher colostrum yield than control (7.01 and 5.12 kg, p<0.05). The concentration of IgG in colostrum at 24 h in the PGF2α group was higher than the control (31.6 and 17.4 g/L, p<0.05). For primiparous sows, milk yield was highest in the sows treated with multiple doses of PGF2α during lactation and lowest in control sows (10.25 and 7.61 kg, p<0.05). Colostrum intake was higher in the treatment than the control groups (+56.7 g, p<0.05). Primiparous sows treated with multiple doses of PGF2α had a higher litter weight than controls (p<0.01). Conclusion: Postpartum treatment with PGF2α improved colostrum yield and IgG in multiparous sows and increased colostrum intake of piglets. Multiple administration of PGF2α improved the milk yield and increased litter weight of piglets in primiparous sows.