• Korean Journal of Soil Science and Fertilizer
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• v.44 no.2
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• pp.293-296
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• 2011

This survey was conducted to promote the environment-friendly use and recycling of livestock feces by obtaining information about the current state of livestock feces composts manufactured in Gyeonggi Province. Therefore, some aspects of quality and manufacturing techniques of livestock feces composts (LFCs) were examined especially in relation to the LFCs quality standard (LQS). By surveying the 70 composting plants in Gyeonggi Province, the total commercial production of LFCs in 2008 was estimated to be about $480,000Mg\;year^{-1}$ and they were manufactured mainly by using both mechanical mixer and bottom air blower. LFCs were composed mainly of chicken feces 29.2%, pig+chicken feces 23.1%, pig feces 20.0%, livestock feces+oil cake 12.3%, pig+chicken+cattle feces 10.8% and pig+cattle feces 4.6%. On the basis of the current official standard which was revised on March 2010, 11 composts out of surveyed 76 ones did not meet the LQS due to inadequate content of water (5), OM/N (1), NaCl (2) and Zn (3). The satisfaction rate to LQS by manufacturers was 100% in the composts produced by farmer's cooperative societies, 80.7% by civil factories, and 44.4% by farming guilds, respectively. The OM/N declined by adding chicken feces and oil cake, while Ca content was increased by the addition of chicken feces and NaCl was increased by adding cattle feces.

• Journal of Biosystems Engineering
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• v.38 no.2
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• pp.121-128
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• 2013

Purpose: Porcine proliferative enteropathy (PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. In order to diagnose PPE rapidly, the rapid kit was developed and tested. Methods: In this study, a rapid kit was developed to screen the PPE rapidly at the pig farm. Also, occult blood test with fecal occult blood (FOB) kit was done for detecting the blood in pig feces which might be the evident of hemorrhagic PPE. For developing the kit, we tested fecal samples of PPE infected pigs diagnosed by polymerase chain reaction (PCR) method. Results: With the developed rapid kit, Lawsonia intracellularis was detected in high density emulsion of ileum. On the other hand, the test result of detecting Lawsonia in feces showed too high non-specific response. In addition, nevertheless the FOB test result showed that blood evident could be founded in pig feces, the diagnosing result was not fit to PCR test result, which shows blood in pig feces could be from not only hemorrhagic PPE but also many reasons. Conclusions: To deal with the PPE effectively, it will be better for farmers to screen the PPE in earlier stage with easy and rapid diagnosing tool on farm. This study found out that the rapid kit could detect the Lawsonia intracellularis and hemoglobin in pig feces. However, the non-specific response to negative samples of PPE was too high to use at a pig farm. Further research is needed for lowering the non-specific response with the rapid kit.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.9
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• pp.1286-1290
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• 2004

This study was carried out to screen yeasts effective in reducing odor from pig feces. Three isolates from soil and compost sources were selected to treat pig feces. On the basis of morphological and biochemical characteristics, one isolate from compost was Candida rugosa, and two isolates from soil were Candida rugosa and Candida maris. These isolates showed deodorizing activity by reducing the concentration of ${NH}_3$ and R-${NH}_2$. Volatile fatty acids (VFA) are the specific malodorous compounds of pig feces, and the Candida maris from soil showed a 100% reduction of butyric, iso-butyric, and iso-valeric acid in 10% pig slurry medium. However, the Candida rugosa from compost showed a 100% reduction of butyric and iso-butyric acid while that from soil showed a 100% reduction of propionic, butyric acid and iso-valeric acid in the medium. Also, these yeasts were effective in reducing $NH_{4}-N$, soluble-N, and biological oxygen demand (BOD).

• Journal of the Korea Organic Resources Recycling Association
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• v.17 no.4
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• pp.29-35
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• 2009

Pyrolysis is recently used as one of alternative methods of animal waste treatment. In this study bio-oil was produced at $550^{\circ}C$ in an auger reactor through pyrolysis process. Two pig waste mixtures were used, pig feces mixed with rice husks and pig feces mixed with sawdust. The main compositions of hemicellulose, lignin, cellulose, protein, and fat were analyzed chemically. Based on the main composition results obtained, the contents of holocellulose (the sum of hemicellulose and cellulose) and lignin had a significant positive effect on bio-oil production, and there was a significant negative effect of ash content on bio-oil yield. The interactions between the different feedstocks were evaluated, and it was concluded that the interaction between pig feces and rice husks was minimal, whereas the interaction between pig feces and sawdust was significant.

• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1701-1708
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• 2021

Food safety is the most important global health issue due to foodborne pathogens after consumption of contaminated food. Foodborne bacteria such as Escherichia coli, Salmonella enterica, Staphylococcus aureus, Campylobacter spp., Bacillus cereus, Vibrio spp., Yersinia enterocolitica and Clostridium perfringens are leading causes of the majority of foodborne illnesses and deaths. These foodborne pathogens often come from the livestock feces, thus, we analyzed fecal microbial communities of three different livestock species to investigate the prevalence of foodborne pathogens in livestock feces using metagenomics analysis. Our data showed that alpha diversities of microbial communities were different according to livestock species. The microbial diversity of cattle feces was higher than that of chicken or pig feces. Moreover, microbial communities were significantly different among these three livestock species (cattle, chicken, and pig). At the genus level, Staphylococcus and Clostridium were found in all livestock feces, with chicken feces having higher relative abundances of Staphylococcus and Clostridium than cattle and pig feces. Genera Bacillus, Campylobacter, and Vibrio were detected in cattle feces. Chicken samples contained Bacillus, Listeria, and Salmonella with low relative abundance. Other genera such as Corynebacterium, Streptococcus, Neisseria, Helicobacter, Enterobacter, Klebsiella, and Pseudomonas known to be opportunistic pathogens were also detected in cattle, chicken, and pig feces. Results of this study might be useful for controlling the spread of foodborne pathogens in farm environments known to provide natural sources of these microorganisms.

• Journal of Biosystems Engineering
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• v.37 no.6
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• pp.420-428
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• 2012

Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods : To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results : The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and 50 PPE negative samples. Conclusions : The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis.

• Journal of Sericultural and Entomological Science
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• v.34 no.1
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• pp.8-14
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• 1992

To investigate the effects of applying animal feces to the mulberry field on the chemical properties and mulberry leaf yield, poultry, pig and cattle feces were applied to the mulberry field making-up the sand loam from 1988 to 1990. The chemical properties of the mulberry field have been improved by the application of the poultry, the pig, and the cattle feces, increasing pH level, organic matter, P2O5 and exchangeable cation, especially Mg and K. Applying the pig feces and poultry feces to the mulberry field increased leaf yield during autumn rearing season, but not increased during spring rearing season. Applying animal feces to the mulberry field increased the content of total-cargbohydrate in the leaf in autumn.

• Microbiology and Biotechnology Letters
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• v.9 no.4
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• pp.219-223
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• 1981

Experiments on methane gas digestion were conducted to prevent livestock pollution and develop substitute energy. When about 30(w/w)% of sludge was added to cow feces, pig feces, and poultry feces and digested at 37$^{\circ}C$ for 20 days, methane gas produced per Kg of organic matter for cow feces, pig feces, poultry feces was 131, 248 and 235 l, respectively. pH decreased slightly at first but increased gradually afterwards during digestion period. When 20, 30, and 40(w/w)% of sludge were added to the mixture of cow feces (300g) and water (200g), the volumes of gas produced were 6.1, 14.5 and 13.4 l, respectively. Volume of methane gas produced from the mixture of cow feces and saw dust was much more than that from the mixture of cow feces and rice polishings. The contents of N, K, P for digestion residues were sufficient to be utilized as a fertilizer. When methane gas digestion was carried out with cow feces in a submersible pump digester the volume of methane gas produced per Kg of organic matter was 188 l. The price of total methane gas produced at this digestion was similar to that of the electric power consumed.

• Journal of Animal Environmental Science
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• v.8 no.1
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• pp.17-24
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• 2002

This study was carried out to isolate and identify the yeasts from the the composts, which were effective to reduce odor of the pig feces, and to investigate their physiological properties. In yeasts, one of 30 isolates was obtained on 10% pig feces extract medium. Judging from the morphological and biochemical characteristics, the CY-10 isolated from the compost were identified as Candida rugosa. This isolated strain showed the deodorizing activity by reducing the concentration of $NH_3$ and R-$NH_2$ than that of the control. The CY-10 had completely utilizing butyric acid and iso-butyric acid including 10% pig slurry of the volatile fatty acids, which are the specific malodorous agents of pig feces. Compared to control, this yeast was found effective for decrease in $NH_4$-N, Soluble-N and BOD, 20%, 12.6%, and 9.82% respectively.

• Korean Journal of Veterinary Service
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• v.24 no.1
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• pp.43-50
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• 2001

Swine proliferative enteritis(SPE) caused by inかsoma intracellularis is a common enteric disaese of grower and finisher pig. Swine affected with SPE show variable clinical signs including diarrhea, weight loss, aberrant growth and death. The characteristic lesion of ileitis at necropsy is marked thickening of the last section of the small intestine. The inner lining of the thickened intestine proliferates almost like a cancer and curved rod bacteria(L intracellularis) are always seen inside the intestinal wall. Infected swine shed the organism in the feces. Isolation and growth of pure L intracellularis in vitro requires a suitable cell culture. This procedure is difficult and not a practical means of diagnosis, thus the polymerase chain reaction(PCR) test of feces can be used to determine whether a pig is shedding the infective organism. A sensitive assay based on amplification of a 319bp DffA fragment of the L intracellularis of Swine proliferative enteritis was attempted for the detection of the organism in the 62 feces of swine. L intracellularis was identified on three herds and detected in 6 fecal samples, representing a infection rate of 9.7%. The PCR was very sensitive and specific on the individual level. The PCR technique could be very useful for the diagnosis of this disease.