• 제목/요약/키워드: Pieris rapae

검색결과 59건 처리시간 0.023초

배추흰나비(Pieris rapae L.)의 위심세포(圍心細胞)에 관한 연구 (A Study of the Pericardial Cell on the Cabbage Butterfly, Pieris rapae L.)

  • 김창환;김우갑;이근옥
    • Applied Microscopy
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    • 제19권1호
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    • pp.34-48
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    • 1989
  • The oval shaped pericardial cells are clustered along the lateral sides of the heart and irregularly connected with the heart. The cells are bounded by a basement membrane. The basement membranes of the connected two peicardial cells are irregularly linked each other there-fore funnels are formed. The multiple invaginations of the cell membrane are observed and septate junctions develope at the part of enterance of the cell membrane. The coated pits are appeared in the inner side of the invaginated cell membrane. The coated vesicles, tubular and spherical shaped vesicle, Golgi complex containing high electron densed material in the cisternae and mitochondria are observed in the cytoplasm and lysosomes are remarkably well developed. The whirled membrane structures in the multiformed complex bounded by single membrane are linked with low electron densed granules and spherical shaped small granules having high electron density with $0.03{\mu}m$ in diameter are located between the whirled membrane in a row and gradually secretes the granules and then they produced the multilamellar body. The lysosomal regions of cytoplasm of pericardial cell are appeared negative reaction to the acid phosphatase and according to the results of the electrophoresis, lipoproteins having acid phosphatase activity are contained. The axon is contacted with the pericardial cells.

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배추흰나비의 變態에 EK른 中腸內 蛋白質分解酵素 (Proteolytic Enzyme in the Midgut during Metamorphosis of Pieris rapae L)

  • Kim, Hak-Ryul;Yoe, Sung-Moon
    • 한국동물학회지
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    • 제25권2호
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    • pp.63-70
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    • 1982
  • 배추흰나비의 變態에 따른 中腸內 蛋白質分解酵素의 變化, 分布 및 性質을 調査 分析하였다. 中腸內 蛋白質分解酵素는 5齡 幼蟲과 前 期에서 變態直前에 높은 活性이 나타났으나  時期에는 變態後 1日에 가장 높았다. 또한 中腸內의 optimum pH는 5齡 幼蟲에서 pH 8, 前 期에서 pH 6.5, 羽化直後에 pH 8.5로 각각 相異하게 나타났다. 細胞內 酵素 分布는 5齡에서는 주로 中腸의 組織에서 發見되었으나 이후 羽化直前까지는 주로 中腸 內容物에서 發見되었고 中腸 組織에서는 거의 식별할수 없었다. 이러한 結果로 보아 蛋白質分解酵素는 幼蟲期동안 中腸組織에서 合成되였다가  化期에 中腸 內腔으로 放出되는 것 같다.

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Lepidoptera 추출물에 의한 항산화 및 Cox-2 프로모터 활성 비교 (Comparison of Anti-Oxidative and Cox-2 Promoter Activities of Lepidoptera Extracts)

  • 손형우;허진철;이상한
    • 한국식품저장유통학회지
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    • 제17권5호
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    • pp.752-756
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    • 2010
  • 나비류 추출물은 전통적으로 다양한 활성을 보유하여 의약용으로 사용되어졌다. 5종의 나비(산제비나비[Papilio maackii], 호랑나비[Papilio xuthus], 배추흰나비[Pieris rapae], 남방호랑나비[Eurema hecabe], 왕오색나비[Sasakia charonda])를 이용하여 물, dimethly sulfoxide (DMSO), ehtanol 및 methanol로 추출한 추출물로 항산화 활성 및 Cox-2 promoter assay를 수행하였다. 그 결과 산제비나비의 추출물이 전반적으로 높은 항산화활성을 나타내었으며, Cox-2 promoter assay에서는 호랑나비의 DMSO 분획이 가장 저해활성이 높은 것으로 나타났다. 활성이 높은 추출물을 대상으로 분리 및 정제를 통하여 유용한 식의약 소재로의 기초연구가 더 필요하다.

배추흰나비 5령유충의 뇌신경내분비계의 구조 (Architecture of Cerebral Neuroendocrine System in the Lawa of Cabbage Butterfly Pieris rapue)

  • 이봉희;윤혜련심재원
    • 한국동물학회지
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    • 제36권2호
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    • pp.285-292
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    • 1993
  • This investigation has been carried out to clarify structural architecture of cerebral neuroendocrine systems in the fifth instar lanra of cabbage butterfly Pieris rapae. In order to examine the cerebral neurosecretorv cell systems the brain and retrocerebral neuroendocrine complex were histochemically stained with the paraldehvde fuchsin. The brain of the fifth instar laMa contains three kinds of neurosecretorv cells: medial, lateral and tritocerebral neurosecretorv cells. The axon bundles of medial and lateral neurosecretory cells form medial neurosecretory pathway(MNSP) and lateral neurosecretorv pathwav(LNSP) within the brain respectively. Especially, prior to exiting the brain, the axon bundles of medial neurosecretorH cells located in both left and right cefebral hemispheres decussate in cerebral medial region and project to contralateral retrocerebral neuroendocrine complexes. Outside the brain the axon bundles of medial and lateral neurosecretory cells form the nenri corporis cardiaca(NCC) I and II respectively. The NCC I and ll run together to the retrocerebral neuroendocrine complex, forming the large nenre bundles in both left md right sides. The anon bundles of tritocerebral neurosecretory cells which pass through the brain along the tritocerebral neurosecretory pathway (TNSP) form the Ncc III outids the train. some of the Ncc I and it terminate in the corpus cardiacum, while the others pass through the corpus cardiacum without termination. The nerve bundle which passes the corpus cardiacum forms the nenrus corforis allatum(NCA) I which runs between the corpus cardiacum and the corpus allatum. Theyt are finally innervated to the corpus allatum. The Ncc III Projects to the corpus cardiRcum. However, most of NCC III priss through the corpus cardiacum without branching and then run down for another organ.

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배추벌레 5령유충의 식도하신경절에 분포하는 세로토닌 면역반응성 신경망의 구조 (Immunocvtochemical Localization of Serotonergic Neurons in Suboesophageal Ganglion of Cabbage Worm Pieris rapae (Insecta, Lepidoptera))

  • 심재원;이봉희
    • 한국동물학회지
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    • 제36권1호
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    • pp.116-122
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    • 1993
  • An immunocytochemical investigation has been carried out to localize serotoninimmunoreactive (5-HTi) neurons in suboesophageal ganglion of fifth instar lawn of cabbage worm Pieris rupae. The 285-HTi cell bodies were identified in the rind of suboesophaseal ganglion. The four 5-HTi cell bodies of them are 18rge in size (about 35 Um), while the remaining cell bodies are medium-sized (about 15 Uml. The 5-HTi nerve processes are abundantly located in central large neuropil, circumoesophageal connectives which join the suboesophaseal ganglion to the tritocerebrum of the brain, and connectives between the suboesophageal and the first thoracic ganglia. These results indicate that the 5-HTi nerve fibers, which constitute the central large neuropil, have structural connections with the above two connectives. Especially in central large neuropil, many 5-HTi nenre fibers form a large circular bundle, in which a 5-HTi nerve fiber bundle is crossing.

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배추흰나비 (Pieris rapae L.) 뇌간부(腦間部)의 신경세포(神經細胞)에 대한 미세구조(微細構造) (Ultrastructure of Nerve Cells in the Pars Intercerebralis of Cabbage Butterfly Pieris rapae L.)

  • 이봉희;김우갑
    • Applied Microscopy
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    • 제12권2호
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    • pp.55-68
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    • 1982
  • The study on the nerve cells in the pars intercerebralis(IP) of 5-day-old cabbage butterfly Pieris rapae L. was performed to observe their ultrastructures and classify them on the basis. of the differences in size, shape and relative distribution cf cell organelles. The brain-subesophageal ganglion complex was fixed in 1% paraformaldehyde-1% gluaraldehyde mixture and embedded in araldite mixture. The transverse thin sections of IP were stained with uranyl acetate and lead citrate and examined by Hitachi 500 and ]EM 100B electron microscope. Five distinct types. of nerve cells are recognized and are arbitrarily designated as Type I, Type II Type III, Type IV and Type V. Type I neurone: These neurones are neurosecretory cells. Several neurosecretory cells are. recognized in the pars intercerebralis. They are roughly round or peach-shaped cells measuring $13{\sim}25{\mu}m$ in diameter. The rounded nucleus shows about $5{\sim}10{\mu}m$ in diameter. The chromatin is predominantly diffused with only occasional dense patches. The perikaryon contains numerous. mitochondria, free polyribosomes and neurosecretory granules. The neurosecretory granules are relatively uniform in electron density, and each one is about $100{\sim}400{\mu}m$ in diameter and surrounded by a single membrane. The granules are also observed mostly as in groups. In one group of neurones the cisternae of endoplasmic reticulum are distended or in other group of neurones are not distended. Golgi saccules are slightly dilated at their lateral extremities and contains. frequenty dense rounded materials. Type II neurone: Thes have the largest soma in the pars intercerebralis about $30{\sim}35{\mu}m$ in diameter. They also show roughly polygonal in shape. The nucleus is elongated or sickle-shaped. The chromatin is mainly in the euchromatin form. The perikarya in these cells are well populated with populated with free ribosomes and contain numerous mitochondria and Golgi bodies. The cisternae of granular endoplasmic reticulum are also well distributed. Type III neurone: They are oval or spindle-shaped and also medium-sized. neurones approximately $15{\sim}17{\mu}m$ in length. The nucleus is oval or slightly elongated in shape and $8{\sim}9{\mu}m$ in length. The chromatin occurs in diffused form. The cytoplasm contains many filamentous or oval mitochondria. The perikaryon has also numerous free polyribosomes and cisternae of granular endoplasmic reticulum. Type VI neurone: They are roughly polygonal in shape probably due to the close approximation of the adjacent cells. The soma is about $7{\sim}8{\mu}m$ in diameter. The nucleus is round or oval in shape and $5.0{\sim}5.8{\mu}m$ in diameter. The necleus also occupies a large proprion of the cell body. The perikaryon is well populated with free ribosomes and contains several mitochondria and cistenae of granular endoplasmic reticulum. Type V neurone: These neurones are similar to Type VI neurones in various respects such as cell size and cell inclusion, but they differ from Type IV neurones in shape. The soma is oval or slightly elongated. The cell body contains several filamentous and oval mitochondria.

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