• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• 제20권2호
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• pp.71-77
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• 2015

High-Performance Liquid Chromatography (HPLC) is a widely used method for measuring the concentration of chlorophyll a as an indicator for estimating phytoplankton biomass and primary production and also for identifying carotenoids to determine phytoplankton composition. However, tissue grinding procedure requires a lot of time and experience in the analysis of multiple sample. Accordingly, we measured the concentrations of photosynthetic pigments before and after the grinding, in order to understand the grinding effects on the quantitative analysis of chlorophylls and carotenoids using samples from southwestern East Sea. When tissue grinding procedure was omitted, we found that Chl a concentrations were underestimated up to 45% in average. Also, concentrations of Zeaxanthin, 19'-butanoyloxyfucoxanthin, 19'-hexanoyloxyfucoxanthin, biomarkers of pico and nano-size phytoplankton, were underestimated up to maximum 77~85% without grinding. We found that the smaller the phytoplankton, the bigger underestimation of their biomarker pigments concentration is likely to happen due to the incomplete extraction. Thus, tissue grinding procedure should be included for HPLC analysis in all cases, to prevent the underestimation of not only Chl a but also carotenoids pigments.

• Proceedings of the KSRS Conference
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.317-322
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• 2002

Dramatic changes in the patterns of satellite-derived pigment concentrations, sea-level height anomaly, sea surface temperature anomaly, and zonal wind anomaly are observed during the 1997-1998 El Nino. By some measures, the 1997-1998 El Nino was the strongest of the 20$^{th}$ century. A very strong El Nino developed during 1997 and matured late in the year. A dramatic recovery occurred in mid-1998 and led to a La Nina conditions. The largest spatial extent of the phytoplankton bloom was followed recovery from El Nino over the equatorial Pacific. The evolution towards a warm episode (El Nino) continued in the equatorial Pacific from March 2002 and further development toward mature El Nino conditions may be possible in late 2002. The OSMI (Ocean Scanning Multispectral Imager) data can be used for detection of dramatic changes in the patterns of pigment concentration during next El Nino.

• Proceedings of the KSRS Conference
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.1413-1414
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• 2003

Interannual variability in the patterns of satellitederived pigment concentrations, sea-level height anomaly, sea surface temperature anomaly, and zonal wind anomaly are observed during the 2002-2003 El Ni${\tilde{n}}$o. The largest spatial extent of the phytoplankton bloom was recovery from El Ni${\tilde{n}}$o over the equatorial Pacific. The evolution towards a warm episode (El Ni${\tilde{n}}$o) started from spring of 2002 and continued during January 2003, while equatorial Sea Surface Temperature Anomaly (SSTA) remained greater than +1$^{\circ}$C in the central equatorial Pacific. The EOS (Earth Observing System) and OSMI (Ocean Scanning Multispectral Imager) data are used for detection of dramatic changes in the patterns of pigment concentration during El Ni${\tilde{n}}$o.

• Proceedings of the KSRS Conference
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• 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume II
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• pp.1019-1022
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• 2006

Satellite sea ice data from 1978 to the present reveal that the perennial ice (or ice that survives the summer) has been rapidly declining at almost 10% per decade. Warming due to increases in greenhouse gases in the atmosphere is now also being reflected in winter with drastic reductions in the maximum extent observed in 2005 and 2006. The retreat of the perennial ice also exposes more open water and has revealed an asymmetric distribution of chlorophyll a pigment concentration in the Arctic basin. Phytoplankton blooms are most dominant at high latitudes, partly on account of sea ice, but in the Arctic basin, it appears that pigment concentrations in the Eastern (Laptev Sea) Region are on the average three times higher than those in the Western (Beaufort Sea) Region. Such asymmetry suggests that despite favorable conditions provided by the melt of sea ice, there are other factors that affects the productivity of the region. The asymmetry is likely associated with much wider shelf areas in the East than in the West, with sea ice processes that inhibits the availability of nutrients near the surface in deep water regions, and river run-off that affects nutrient availability. The primary productivity in the pan-Arctic region have been estimated using the pigment concentrations and PAR derived from SeaWiFS data and the results show large seasonal as well as interannual variability during the 1998 to 2005 period. The data points towards increasing productivity for later years but with only 9 years of data it is too early to tell the overall effect of the sea ice retreat.

• Journal of Environmental Science International
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• 제23권12호
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• pp.2015-2028
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• 2014

We analyzed with HPLC (High Performance Liquid Chromatography) analysis photosynthetic pigments and environmental factors, microscopic observations of the phytoplankton and zooplankton in the seawater every month from February 2009 to November 2010 in Haengam Bay. The level of dissolved inorganic nutrients was the highest between July and September, when freshwater influx was at its peak, whereas chlorophyll a levels were the highest in April and August. Also, phytoplankton pigment concentration increased when dissolved inorganic nutrients are carried into nearshore waters by rainfall runoff. Based on identification of phytoplankton and photosynthetic pigments results, diatoms were mainly dominant while dinoflagellate populations increased at July and August 2009, May 2010. The zooplankton communities are dominated in terms of Noctiluca scintillans. The contribution of Noctiluca scintillans in 2010 accounts for approximately 77.3% of the total zooplankton. Distribution patterns over time of zooplankton in the seasonal distribution of phytoplankton showed a different pattern.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• 제17권2호
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• pp.87-94
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• 2012

To compare monthly variations of phytoplankton biomass and community composition between in seawater and sediment of the Gomso Bay (tidal flat: approximately 75%), the photosynthetic pigments were analyzed by HPLC every month in 1999 and every two months in 2000. Ambient physical and chemical parameters (temperature, salinity, nutrients, dissolved oxygen, and chemical oxygen demand) were also examined to find the environmental factors controlling structure of phytoplankton community. The temporal and spatial variations of chlorophyll a concentration in seawater were correlated well with the magnitude of freshwater discharge from land. The biomass of microphytobenthos at the surface sediments was lower than that in other regions of the world and 2-3 times lower than phytoplankton biomass integrated in the seawater column. Based on the results of HPLC pigment analysis, fucoxanthin, a marker pigment of diatoms, was the most prominent pigment and highly correlated with chlorophyll a in seawater and sediment of the Gomso Bay. These results suggest that diatoms are the predominant phytoplankton in seawater and sediment of the Gomso Bay. However, the monthly variation of chlorophyll a concentration in seawater at the subtidal zone was not a good correlation with that in sediment of the Gomso Bay. Although pelagic plankton was identified in seawater by microscopic examination, benthic algal species were not found in the seawater. These results suggest that contribution from the suspended microphytobenthos in the tidal flat to the subtidal zone of the Gomso Bay may be low as a food source to the primary consumer in the upper water column of the subtidal zone. Further study needs to elucidate the vertical and horizontal transport magnitude of the suspended microphytobenthos in the tidal flat to the subtidal zone.

• Korean Journal of Environmental Biology
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• 제34권1호
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• pp.18-29
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• 2016

The effects of water temperature, salinity, water column nutrient contents, and phytoplankton primary productivity on pigment composition and concentration, as well as primary productivity of Pyropia yezoensis Ueda purple lavers were studied at the primary cultivation areas in the Manho (Jindo-Haenam) region on the southwestern coast of Korea in March 2014. The water temperature was $9.1{\sim}9.6^{\circ}C$, salinity was 32.5~33.1, and transparency was 0.7~1.5 m. The shallow euphotic depth resulted from the high turbidity. Water column dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), and silicate concentrations were $3.59{\sim}5.73{\mu}M$, $0.16{\sim}0.41{\mu}M$, and $12.41{\sim}13.94{\mu}M$, respectively. Chlorophyll a (Chl a) concentration was $0.51{\sim}1.25{\mu}g\;L^{-1}$. Nanoplankton ($0.7{\sim}20{\mu}m$ size class) accounted for 58% of the total Chl a concentration. Fucoxanthin was the dominant photosynthetic pigment at all sites. Microplankton ($20{\sim}200{\mu}m$ size class) accounted for 64% of the total fucoxanthin concentration. The primary productivity of phytoplankton was $57.72{\pm}4.67(51.05{\sim}66.71)mg\;C\;m^{-2}d^{-1}$. The nanoplankton ($0.7{\sim}20{\mu}m$ size class) accounted for 77% of the total phytoplankton primary productivity. The calculated phytoplankton primary productivity was $11,337kg\;C\;d^{-1}$. The primary productivity of Pyropia blades was $1,926{\pm}192(1,102{\sim}2,597)mg\;C\:m^{-2}d^{-1}$, i.e., calculated as $39,295kg\;C\;d^{-1}$. The total primary productivity of phytoplankton and Pyropia blades was $50,632kg\;C\;d^{-1}$. The primary productivity of Pyropia blades was 3.5 times greater than that of phytoplankton in the Manho region on the southwestern coast of Korea.

• Korean Journal of Remote Sensing
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• 제36권6_1호
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• pp.1339-1348
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• 2020

Phytoplankton controls marine ecosystems in terms of nutrients, photosynthetic rate, carbon cycle, etc. and the degree of its influence on the marine environment depends on their physical size. Many studies have been attempted to identify marine phytoplankton size classes using the remote sensing techniques. One of successful approach was the three-component model which estimates the chlorophyll concentrations of three phytoplankton size classes (micro-phytoplankton; >20 ㎛, nano-; 2-20 ㎛ and pico-; <2 ㎛) as a function of total chlorophyll. Here, we examined the applicability of Geostationary Ocean Colour Imager (GOCI) to the mapping of the phytoplankton size class distribution in the East Sea. A fit of the three-component model to a biomarker pigment dataset collected in the study area for some years including a large harmful algal bloom period has been carried out to derive size-fractioned chlorophyll concentration (CHL). The tuned three-component model was applied to the hourly GOCI images to identify the fractions of each phytoplankton size class for the entire CHL. Then, we investigated the distribution of phytoplankton community in terms of the size structure in the East Sea during the harmful Cochlodinium polykrikoides blooms in the summer of 2013.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 한국해양정보통신학회 2004년도 SMICS 2004 International Symposium on Maritime and Communication Sciences
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• pp.43-48
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• 2004

A pilot-scale system of 20t/h for the treatment of ship's ballast water and the setup of dissolved hydroxyl radical was introduced in this paper. With this experimental system, the kill efficiencies of bacteria, mono-algae, protozoan reach 100% within 2.67s when dissolved OHㆍconcentration is 0.6mg/L. At the same time, the effect of hydroxyl radicals on the photosynthesis pigments of phytoplankton was done. The results indicate that the contents of chi-a, chl-b, chl-c and carotenoid are decreased to 35~64% within 8.0s further to the lowest limit of test after 5 minutes. When dissolved OHㆍratio concentration is 0.68mg/L, the attenuation efficiencies of photosynthesis pigment are 100%. Therefore the invasive marine species can be killed in the process of the inputting and discharge ship's ballast water.

• Journal of Environmental Impact Assessment
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• 제10권1호
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• pp.1-8
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• 2001

Detection of red tides by satellite remote sensing can be done either by detecting enhanced level of chlorophyll pigment or by detecting changes in the spectral composition of pixels. Using chlorophyll concentration, however, is not effective currently due to the facts: 1) Chlorophyll-a is a universal pigment of phytoplankton, and 2) no accurate algorithm for chlorophyll in case 2 water is available yet. Here, red band algorithm, classification and PCA (Principal Component Analysis) techniques were applied for detecting patches of Cochlodinium polykrikoides red tides which occurred in Korean waters in 1995. This dinoflagellate species appears dark red due to the characteristic pigments absorbing lights in the blue and green wavelength most effectively. In the satellite image, the brightness of red tide pixels in all the three visible bands were low making the detection difficult. Red band algorithm is not good for detecting the red tide because of reflectance of suspended sediments. For supervised classification, selecting training area was difficult, while unsupervised classification was not effective in delineating the patches from surrounding pixels. On the other hand, PCA gave a good qualitative discrimination on the distribution compared with actual observation.