• Asian-Australasian Journal of Animal Sciences
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• v.28 no.9
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• pp.1317-1326
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• 2015

This study investigated the metabolizable energy (ME) intake, net energy of production (NEp), heat production (HP), efficiencies of ME use for energy, lipid and protein retention as well as the performance of broiler chickens fed diets based on cassava chips or pellets with or without supplementation with an enzyme product containing xylanase, amylase, protease and phytase. The two products, cassava chips and pellets, were analysed for nutrient composition prior to feed formulation. The cassava chips and pellets contained 2.2% and 2.1% crude protein; 1.2% and 1.5% crude fat; and 75.1% and 67.8% starch, respectively. Lysine and methionine were 0.077%, 0.075%, and 0.017%, 0.020% protein material, respectively, while calculated ME was 12.6 and 11.7 MJ/kg, respectively. Feed intake to day 21 was lower (p<0.01) on the diet containing cassava chips compared to diets with cassava pellets. Enzyme supplementation increased (p<0.01) feed intake on all diets. Live weight at day 21 was significantly (p<0.01) reduced on the diet based on cassava chips compared to pellets, but an improvement (p<0.01) was noticed with the enzyme supplementation. Metabolizable energy intake was reduced (p<0.01) by both cassava chips and pellets, but was increased (p<0.01) on all diets by enzyme supplementation. The NEp was higher (p<0.01) in the maize-based diets than the diets containing cassava. Enzyme supplementation improved (p<0.01) NEp in all the diets. Heat production was highest (p<0.01) on diets containing cassava pellets than on cassava chips. It is possible to use cassava pellets in diets for broiler chickens at a level close to 50% of the diet to reduce cost of production, and the nutritive value of such diets can be improved through supplementation of enzyme products containing carbohydrases, protease, and phytase.

• Asian-Australasian Journal of Animal Sciences
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• v.22 no.3
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• pp.404-409
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• 2009

A total of 240 day-old broiler chicks were used to study the effects of phytase on performance and phosphorus (P) excretion from birds fed diets containing low phytate barleys formulated without inorganic P. A positive control based on Harrington barley (HB) was formulated to meet requirements for total P. Three experimental diets, based on either HB (0.39% total P with 0.28% phytate P) or the low phytate varieties LP 422 (0.36% total P with 0.14% phytate P) and LP 955 (0.40% total P with 0.01% phytate P), were formulated to be below requirements for total P by removing all the inorganic P from the diet. The four diets were fed with and without 1,000 FTU/kg phytase. Apparent P digestibility was significantly higher (p<0.01) for birds fed the low phytate barleys than for birds fed HB either supplemented or un-supplemented with inorganic P. P excretion was significantly lower (p<0.01) for birds fed HB without inorganic P than with inorganic P. P excretion was further reduced by the use of the low phytate barleys LP 422 and LP 455 (p<0.01). Phytase supplementation did not affect P excretion (p = 0.39). Body weight gain and feed intake were highest for birds fed the HB diet with inorganic P and lowest for birds fed the HB diet without inorganic P (p<0.01). Among the three low P diets, body weight gain and feed intake of broilers increased as the level of phytate in the barley declined (p<0.01). Phytase modestly increased body weight gain (p = 0.08) and feed intake (p = 0.04). The overall results of this study indicate that it may be possible to reduce the amount of inorganic P used when formulating diets with low phytate barley compared with the levels needed when formulating diets with normal phytate barley. However, it is not possible to completely replace the inorganic P in diets containing low phytate barley without impairing poultry performance. Feeding diets devoid of supplementary inorganic P in combination with low phytate barley resulted in a significant reduction in P excretion by poultry.

• Asian-Australasian Journal of Animal Sciences
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• v.8 no.2
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• pp.135-138
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• 1995

Soybean meal was fermented by Aspergillus usami in order to reduce phytate content. Aflatoxin B1 was not detected in the fermented soybean meal. The contents of crude protein, crude fiber, ether extract and crude ash were slightly increased following fermentation with a concomitant reduction in nitrogen free extract. Though the fermentation partly degraded proteins in the soybean meal, there was small difference in amino acid composition between the soybean meal and the fermented soybean meal. The results showed that the fermentation did not affect nutritional value of protein in soybean meal. Approximately 55% of phosphorus extracted by trichloroacetic acid was inositol hexaphosphate (phytate) in the soybean meal. The content of inositol tetra to hexaphosphates was not detected in the fermented soybean meal. These results indicated that the fermentation almost completely eliminated phytate in soybean meal. Phytase activity was not detected in the unfermented soybean meal. However, the enzyme activity in the fermented soybean meal was 167.7 U/g. When the fermented soybean meal in supplemented in formula feeds, phytase in the fermented soybean meal might partly degrade the phytate in other ingredients in the digestive tract. The fermented soybean meal is possibly used as a phytate-free protein source of feed, which contains high available phosphorus.

• Animal Bioscience
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• v.34 no.7
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• pp.1157-1168
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• 2021

Objective: The aim of this study was to investigate the effects of different amounts of wheat bran (WB) inclusion and postbiotics form by Saccharomyces cerevisiae and phytase co-fermented wheat bran (FWB) on the growth performance and health status of broilers. Methods: Study randomly allocated a total of 300 male broilers to a control and 4 treatment groups (5% WB, 5% FWB, 10% WB, and 10% FWB inclusion, respectively) with each pen having 20 broilers and 3 pens per treatment. Results: The WB does not contain enzymes, but there are 152.8, 549.2, 289.5, and 147.1 U/g dry matter xylanase, protease, cellulase and β-glucanase in FWB, respectively. Furthermore, FWB can decrease nitric oxide release of lipopolysaccharide stimulated chicken peripheral blood mononuclear cells by about two times. Results show that 10% FWB inclusion had significantly the highest weight gain (WG) at 1 to 21 d; 5% FWB had the lowest feed conversion rate at 22 to 35 d; 10% WB and 10% FWB inclusion have the highest villus height and Lactobacillus spp. number in caecum; and both 5% and 10% FWB can increase ash content in femurs. Compared to control group, all treatments increase mucin 2, and tight junction (TJ), such as occludin, claudin-1, zonula occludens-1, and mRNA expression in ileum by at least 5 folds. In chicken peripheral blood mononuclear cells, nicotinamide adenine dinucleotide phosphate-oxidase-1 mRNA expression decreases from 2 to 5 times, and glutamate-cysteine ligase catalytic subunit mRNA expression also increases in all treatment groups compared to control group. The mRNA expression of pro-inflammatory cytokines, including interleukin-6 (IL-6), nuclear factor-κB, and IL-1β, decreases in 5% and 10% FWB groups compared to control group. Conclusion: To summarize, both WB and FWB inclusion in broilers diets increase TJ mRNA expression and anti-oxidation and anti-inflammation, but up to 10% FWB groups have better WG in different stages of broiler development.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.317-317
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• 2003

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.3
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• pp.432-444
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• 2001

In this manuscript, several effective feeding and management systems to reduce environmental pollution in swine production have been briefly introduced. It is logical that reducing the excretion of nutrients in manure should be the first step to reduce the environmental impact of pig production. it is evident that the excretion of nitrogen and phosphorus can be reduced when more digestible or available feedstuffs are used. Also, it is well known that proper feed processing can reduce anti nutritional factors (ANF) and improve nutrient digestibilities. Supplementation of effective feed additives can reduce excretion of nitrogen and phosphorus due to efficient feed utilization. These include enzymes (e.g., phytase), antibiotics, probiotics, organic acids and growth hormones ($\beta$-agonists and porcine somatotropin). One of the most effective ways to reduce pollutants from swine manure is to use synthetic amino acids in feed manufacturing. Many studies showed that reduction of 2 to 4% unit (U) of dietary protein with supplemental amino acid (AA) could dramatically reduce (15 to 20%) nitrogen excretion. Regarding feeding strategies, it has been recognized that phase feeding regimen could be used to reduce nitrogen and phosphorous excretion by feeding pigs in better agreement with age and physiological state. Feeding barrows and gilts separately, known as split sex feeding, can also decrease excretion of nitrogen and phosphorus. With the increasing concerns on the negative impact of animal production systems on the environment, animal nutritionists and producers should be aware that sustainability of animal agriculture is as important as high production performance. Therefore, some feeding and management strategies described in this manuscript will help to reduce environmental pollution in swine production. Proper combination of feeding regimen and environment-friendly diet formulation through nutritional approach will be more effective to reduce nutrient excretion in swine production system compared to single approach to do so.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.530-536
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• 2014

In a $2{\times}2$ factorial experiment the hypotheses tested were that the metabolic acid load caused by benzoic acid (BA) added to the feed affects bone mineralization of weanling pigs, and that a wide dietary calcium (Ca) to phosphorus (P) ratio in phytase-supplemented feeds with a marginal P concentration has a positive effect on bone mineralization. The four experimental diets, which contained 0.4% P and were supplemented with 1,000 FTU phytase/kg, contained either 5 g BA/kg or no BA and either 0.77% Ca or 0.57% Ca. The 68 four-week-old Large White pigs were fed the experimental diets ad libitum for six weeks and were then slaughtered. Benzoic acid increased feed intake (p = 0.009) and growth rate (p = 0.051), but did not influence the feed conversion ratio (p>0.10). Benzoic acid decreased the pH of the urine (p = 0.031), but did not affect breaking strength and mineralization of the tibia (p>0.10). The wide Ca:P ratio decreased feed intake (p = 0.034) and growth rate (p = 0.007) and impaired feed the conversion ratio (p = 0.027), but increased the mineral concentration in the fat-free DM of the tibia (p = 0.013) without influencing its breaking strength (p>0.10). The observed positive effect of the wide Ca:P ratio on bone mineralization may be attributed, at least in part, to the impaired feed conversion ratio, i.e. to the higher feed intake and consequently to the higher mineral intake per kg BW gain. The negative impact on animal performance of the wide dietary Ca:P ratio outweighs its potentially positive effect on bone mineralization, precluding its implementation under practical feeding conditions.

• Asian-Australasian Journal of Animal Sciences
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• v.14 no.10
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• pp.1440-1443
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• 2001

Forty eight $Duroc{\times}Yorkshire{\times}Landrace$ pigs ($15.2{\pm}0.3kg$ average initial BW) were used conducted to evaluate the effects dietary phytase (Phytezyme, WOOJIN, Co. Ltd) supplementation on the growth performance and nutrient digestibility in growing pigs. There were three pigs per pen and four pens per treatment. Treatments were 1) Con (corn-wheat-SBM), 2) PE0.1 (low-P iet+0.1% Phytezyme), 3) PE0.2 (Iow-P diet+0.2% Phytezyme), 4) PE0.3 (low-P diet+0.3% Phytezyme). During d 0 to 28, average daily gain was not significantly different among the treatments. Pigs fed PE0.3 diet significantly decreased (p<0.05) their average daily feed intake compared to pigs fed Con diet. Also, gain/feed in pigs fed PE0.1 and PE0.3 diet was improved (p<0.05) compared to pigs fed Con and PE0.2 diet (p<0.05). For d 28 to 56, pigs fed PE0.2 diet grew significantly faster (p<0.05) than pigs fed Con and PE0.1 diet. Gain/feed was greater (p<0.05) for PE0.2 and PE0.3 treatments than for Con. For overall period, average daily gain was increased (P<0.05) by the addition of 0.2% Phytezyme compared with Con. Gain/feed was significantly improved (p<0.05) by supplementation of PE0.3. Pigs fed PE0.2 showed increased DM and N digestibilities compared to pigs fed other treatments. Supplemented diets PE0.2 and PE0.3 improved (p<0.05) the apparent digestibility of Ca and P compared to other treatments. In conclusion, the results obtained from these feeding trials suggest that the Phytezyme supplementation of diets for growing-finishing pigs had improved growth performance and nutrient availability.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.9
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• pp.1198-1204
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• 2010

This study was conducted to investigate the effects of dietary supplementation with multiple enzymes composed of phytase plus carbohydrolases (ROVABIO$^{(R)}$ Max, RM) on growth performance, nutritional availability and intestinal viscosity in broiler chicks. A total of one thousand, one-day-old male broiler chicks were randomly allotted into treatment groups that received one of five experimental diets for 32 days. Each group consisted of 40 birds and all experiments included five replicates. The dietary treatments included PC (a positive control diet), NC1 (65 kcal/kg, 0.15% and 0.10% less ME, available phosphorus and calcium levels, respectively, than the PC diet), NC2 (85 kcal/kg, 0.20% and 0.10% less ME, available phosphorus and calcium levels, respectively, than the PC diet), NC1+RM (NC1 plus ROVABIO$^{(R)}$ Max) and NC2+RM (NC2 plus ROVABIO$^{(R)}$ Max). The average body weights, daily body weight gains and feed conversion rates of the chicks fed a diet containing RM improved significantly or tended to improve. The treatments also had no effect on the carcass characteristics or blood parameters, but the viscosity of the intestinal contents of the chicks fed the diet containing RM was significantly lower than that of chicks in the NC without RM groups. Additionally, chicks fed the dietary RM showed increased breaking strength and ash content of the tibia when compared to chicks that received the non-RM diets. Taken together, the results of the present study indicated that the addition of multiple enzymes consisting of phytase plus NSP enzymes improved the growth performance and mineral status of the tibia in broiler chickens fed corn-wheat-soybean meal-based diets with reduced levels of nutrients. Further, these findings suggest that the improved animal performance is associated with reduced intestinal viscosity by the dietary enzyme complex.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.3
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• pp.386-393
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• 2011

Three trials were conducted to determine the available energy of different wheat screening varieties collected from different locations of Khorasan in Iran. In experiment 1, chemical composition and the nitrogen corrected true metabolisable energy (TMEn) were evaluated. A precision-fed rooster assay was used, in which, each wheat screening sample was tube fed to adult roosters, and the excreta were collected for 48-h. In Exp. 2 and 3, five and two wheat screening verities-based diets with or without xylanase and phytase were fed to 16-day old battery reared chicks respectively, and total feed consumption and excreta were measured during next three days. The variable nature of wheat screening varieties led to significant differences in mean TMEn values (p<0.01). The TMEn values of samples determined with adult roosters varied by ${\pm}5.03%$ of the mean value ($3,097.65{\pm}49.32\;kcal/kg$) and ranged from 2,734.90 to 3,245.12 kcal/kg. There was a significant correlation (p<0.05) between crude fiber (CF), neutral detergent fiber (NDF), and acid detergent fiber (ADF) with TMEn, and the greatest correlation coefficient was observed between NDF and TMEn (r = -0.947; p<0.001). The optimal equation in terms of $R^2$ from using a single chemical analysis was obtained with NDF: TMEn = 4,152.09-27.80 NDF ($R^2$ = 0.90, p<0.0001), and the TME prediction equation was improved by the addition of the crude protein (CP) and ASH content to sequential analysis: TMEn = 3,656.97-28.65 NDF+32.54 CP+38.70 ASH ($R^2$ = 0.98, p<0.0001). The average AMEn values of 5 and 2 wheat screening varieties determined with young broiler chickens were $2,968.41{\pm}25.70\;kcal/kg$ and $2,976.38{\pm}8.34\;kcal/kg$ in Exp. 2 and Exp. 3, respectively. Addition of xylanase and phytase to wheat screenings resulted in significant (p<0.01) improvement in AMEn by 4.21 and 2.92%, respectively.