• 감성과학
• /
• 제12권4호
• /
• pp.557-564
• /
• 2009

현재 한국에서는 거짓말탐지검사의 채점과정에서 '일관성' 기준을 사용하고 있다. 본 연구에서는 현재 한국에서 사용하고 있는 거짓말탐지검사 기법의 채점 기준 중 '일관성' 기준의 실증적 타당성을 검증하고자 하였다. 구체적으로는 한국에서 이루어지고 있는 거짓말탐지검사의 채점과정에서 '일관성'을 실제로 고려하고 있는지의 여부를 검증한 후 '일관성'을 고려하여 채점하는 경우와 그렇지 않은 경우에서 거짓말탐지검사 결과의신뢰도(Cronbach' $\alpha$)와 정확성이 어떻게 달라지는지를 파악하였다. 본 연구는 실제 현장에서 '일관성'을 고려하여 채점 한 원채점자료와 '일관성'을 고려할 수 없도록 가공하여 채점한 실험채점자료를 분석하였다. 원채점자료와 실험채점자료는 동일한 검사관들이 채점하도록 하였다. 그 결과 현재 한국에서는 거짓말탐지검사의 채점과정에서 '일관성'을 고려하고 있는 것이 확인되었다. 신뢰도는 원채점자료가 .93, 실험채점자료가 .91로 비슷한 수준이었다. 정확성은 검찰의 기소 여부와 거짓말탐지검사 점수에 의한 판정을 지표로 사용한 잠재계층분석(N=182)의 결과를 실제 거짓말 여부를 판단하는 기준으로 사용했을 때 원채점자료에서 76.9%, 실험채점자료에서 61.5%로 나타났다. 논의에서는 이러한 결과가 나타난 원인과 한국에서 사용하고 있는 '일관성' 기준이 거짓말탐지검사 채점에서 갖는 유용성에 대하여 논의하였다.

• Journal of Yeungnam Medical Science
• /
• 제30권1호
• /
• pp.10-16
• /
• 2013

Background: Toll-like receptors (TLRs) are well-known pattern recognition receptors. Among the 13 TLRs, TLR2 is the most known receptor for immune response. It activates mitogen-activated protein kinases (MAPKs), which are counterbalanced by MAPK phosphatases [MKPs or dual-specificity phosphatases (DUSPs)]. However, the regulatory mechanism of DUSPs is still unclear. In this study, the effect of a TLR2 ligand (TLR2L, Pam3CSK4) on DUSP4 expression in Raw264.7 cells was demonstrated. Methods: A Raw264.7 mouse macrophage cell line was cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 1% antibiotics (100 U/mL penicillin and 100 g/mL streptomycin) at $37^{\circ}C$ in 5% $CO_2$. TLR2L (Pam3CSK4)-mediated DUSP4 expressions were confirmed with RT-PCR and western blot analysis. In addition, the detection of reactive oxygen species (ROS) was measured with lucigenin assay. Results: Pam3CSK4 induced the expression of DUSP1, 2, 4, 5 and 16. The DUSP4 expression was also increased by TLR4 and 9 agonists (lipopolysaccharide and CpG ODN, respectively). Pam3CSK4 also induced ERK1/2 phosphorylation and ROS production, and the Pam3CSK4-induced DUSP4 expression was decreased by ERK1/2 (U0126) and ROS (DPI) inhibitors. U0126 suppressed the ROS production by Pam3CSK4. Conclusion: Pam3CSK4-mediated DUSP4 expression is regulated by ERK1/2 and ROS. This finding suggests the physiological importance of DUSP4 in TLR2-mediated immune response.

• 한국통신학회논문지
• /
• 제31권10C호
• /
• pp.964-972
• /
• 2006

신장은 간장과 가장 유기적인 관계가 깊은 장기로 혈액 중에서 체내 신진대사 결과 생긴 노폐물을 걸러내 오줌을 만들어 체외로 배출하는 기능을 한다. 그러나 신장은 문제가 발생할 경우 인체가 느끼는 자각증상이 크지 않기 때문에 장기 파손이 상당 부분 진행되어야 그 증상을 알게 된다. 따라서 사회적으로 신장 질환 진단에 대한 중요성이 증대되고 있다. 이를 위해 본 논문에서는 한방의 4대 진단법 중에서 망진과 청진 분야를 이용하여 신장질환에 대한 진단 방법을 제안하고자 한다. 본 논문에서 개발할 시스템은 크게 두 가지로 나누어진다. 하나는 입력 영상에 대한 보정을 통해 정확한 색상 값을 추출하고 최적화된 결과 영상을 통해 신장과 관련된 얼굴에서의 지각 부분의 색을 분석하고 그 값을 이용하여 신장 질환 진단을 하고자 한다. 또 하나는 신장과 음성 신호와의 관계론 비교, 분석하여 이를 입증하는 시스템을 설계하고자 한다. 끝으로 실험을 통해 제안한 방법의 유용성을 입증하고자 한다.

• 약학회지
• /
• 제47권6호
• /
• pp.410-416
• /
• 2003

Autotaxin(ATX) was originally purified from conditioned media of A2058 human melanoma cells and shown to be a potent cell motility-stimulating factor, possessing a type II nucleotide pyrophosphatase/phosphodiesterase (NPP2) activity. Recombinant ATX has recently demonstrated that human plasma lysophosholipase D is identical to ATX and uses lysophosphatidylcholine as a substrate to mediate various biological functions including tumor cell growth and motility through G-protein coupled receptor. However, despite pivotal roles of ATX on physiological or pathophysiological states, the production of ATX is solely depends on complicated purification method which employs multiple column steps, but resulted in very poor yield. This limited the use of ATX for extensive analysis. We, therefore, expressed six histidine-tagged recombinant human ATX(His-ATX) in High Five TM insect cells to improve the generation of ATX and to make simple the purification of ATX. The signal sequence of the human ATX gene was truncated and replaced with sequence of insect cell secretion signal within expression vector. In addition, codons for six histidines were added to the C-termini of 120kDa ATX cDNA construct. A simple purification scheme utilizing two-step affinity column chromatography was designed to purify His-ATX to homogeneity from the culture supernatant of transfected insect cells. Homogenous His-ATX was detected and isolated from the concentrated insect cell medium using concanavalin A agarose and nickel affinity chromatography. Purified His-ATX was in full length with ATX capacity. A combination of this expression system and purification scheme would be useful for production and purification of high-quality functional ATX for research and practical application of multiple functional motogen, ATX/NPP-2.

• 한국자동차공학회논문집
• /
• 제17권4호
• /
• pp.16-23
• /
• 2009

Driving simulators are useful tools not only to test the components of future cars but also to evaluate the telematics service and HMI (Human-Machine Interface). However driving simulators cannot be implemented to test and evaluate the telematics service system because the GPS (Global Positioning System) which contains basic functional support for the telematics module do not work in the VR (virtual reality) environment. This paper presents a method to implement telematics service to a driving simulator by developing the GPS simulator which is able to emulate GPS satellite signals consist of NMEA-0183 protocol and RS232C communication standards. It is expected that the driving simulator with the GPS simulator can be used to study HMI and human-factor evaluations of the commercial telematics system to realize the HiLES (Human-in-the-Loop Evaluation System).

• The Korean Journal of Physiology and Pharmacology
• /
• 제19권6호
• /
• pp.515-522
• /
• 2015

Notch signaling is a key regulator of neuronal fate during embryonic development, but its function in the adult brain is still largely unknown. Mind bomb-2 (Mib2) is an essential positive regulator of the Notch pathway, which acts in the Notch signal-sending cells. Therefore, genetic deletion of Mib2 in the mouse brain might help understand Notch signaling-mediated cell-cell interactions between neurons and their physiological function. Here we show that deletion of Mib2 in the mouse brain results in impaired hippocampal spatial memory and contextual fear memory. Accordingly, we found impaired hippocampal synaptic plasticity in Mib2 knock-out (KO) mice; however, basal synaptic transmission did not change at the Schaffer collateral-CA1 synapses. Using western blot analysis, we found that the level of cleaved Notch1 was lower in Mib2 KO mice than in wild type (WT) littermates after mild foot shock. Taken together, these data suggest that Mib2 plays a critical role in synaptic plasticity and spatial memory through the Notch signaling pathway.

• 설비공학논문집
• /
• 제19권4호
• /
• pp.307-312
• /
• 2007

This study was performed to evaluate sleep efficiencies and conditions for comfortable sleep based on the analysis of sleep efficiency and MST under four thermals conditions ($22^{\circ}C,\;24^{\circ}C,\;26^{\circ}C,\;30^{\circ}C$). Five female subjects who have similar life cycle and sleep patterns were participated for the sleep experiment. Their age was from 20 to 22 years old. They were healthy, and had regular sleep with consistent bed and wakeup time. It was checked whether they had a good sleep before the night of experiment. Experiments were performed in an environmental chamber using thermo-hygrostat. The physiological signal (EEG) for sleep stage were obtained from C3-A2 and C4-Al electrode sites. Sleep stages were classified, then SWS latency and SWS/TST were calculated for the evaluation for sleep efficiencies on thermal conditions. As results, mean skin temperature for comfort sleeping was $34.5{\sim}35.4^{\circ}C$. Considering sleep efficiency and mean skin temperature, indoor room temperature of upper limit was $28.1^{\circ}C$.

• Fisheries and Aquatic Sciences
• /
• 제13권4호
• /
• pp.263-271
• /
• 2010

G protein-coupled receptors (GPCR) constitute the largest superfamily of cell membrane receptors, mediating diverse signal-transduction pathways. The melanocortin 4 receptor (MC4R) has been of interest for its physiological role and size, one of the smallest among the GPCRs, which makes it a good model system for the structural study of GPCRs. To study the molecular structure and tissue-specific expression of MC4R in olive flounder (Paralichthys olivaceus), the full-length MC4R gene was obtained using PCR amplification of genomic DNA as well as cDNA synthesis. Sequence analysis of the gene indicates that 978 bp of the MC4R gene encodes 325 amino acids without introns. Sequence alignment with the MC4Rs from other fish shows the highest degree of identity (96%) between Paralichthys olivaceous and Verasper moseri, followed by Takifugu rubripes and Tetraodon nigroviridis (89%). RNA was isolated from various tissues to examine the tissue distribution of MC4R by using RT-PCR. The results showed major expression of MC4R in the liver, brain, and eye, which is consistent with the expression pattern in other fish belonging to the order Pleuronectiformes.

• 대한의용생체공학회:의공학회지
• /
• 제26권6호
• /
• pp.373-381
• /
• 2005

The respiratory and heart beat signals are the fundamental physiological signals for sleep monitoring in the home. Using the air mattress sensor system, the respiration and heart beat movements can be measured without any harness or sensor on the subject's body which makes long term measurement difficult and troublesome. The differential measurement technique between two air cells is adopted to enhance the sensitivity. The concept of the balancing tube between two air cells is suggested to increase the robustness against postural changes during the measurement period. With this balancing tube, the meaningful frequency range could be selected by the pneumatic filter method. The mathematical model for the air mattress and balancing tube was suggested and the validation experiments were performed for step and sinusoidal input. The results show that the balancing tube can eliminate the low frequency component between two cells effectively. This technique was applied to measure the respiration and heart beat on the bed, which shows the potential applications for sleep monitoring device in home. With the analysis of the waveform, respiration intervals and heart beat intervals were calculated and compared with the signal from conventional methods. The results show that the measurement from air mattress with balancing tube can be used for monitoring respiration and heart beat in various situations.

• 한국감성과학회:학술대회논문집
• /
• 한국감성과학회 1997년도 한국감성과학회 연차학술대회논문집
• /
• pp.113-117
• /
• 1997

본 논문은 여름철 체감실험에 대한 결과이다. 유니폼을 착용한 각 피험자(중학생, 대학생, 고령자)는 90분동안 환경시험실에 체재하면서 의자에 앉아 전신온냉감 및 쾌불쾌감을 신고하였다. 체감실험동안 피부온도는 인체의 3곳에서 측정하였고 피험자는 10분 간격마다 전신온냉감 및 쾌불쾌감을 신고하였다. 환경물리량및 인체 피부온도는 매 20초 간격으로 측정하였다. 여름철 체감실험 결과 아래의 결론을 얻었다. 1)평균피부온도가 증가함에 따라 TSV는 선형적으로 능가하며 열적으로 중립감을 느낄 때으리 평균피부온도는 고령자의 경우 남자 33.8$^{\circ}C$, 여자 34.3$^{\circ}C$이고, 대학생 남자의 경우 34.1$^{\circ}C$ ,여자 33.8$^{\circ}C$, 중학생의 경우는 34.4$^{\circ}C$이다. 2)발한량과 평균피부온도의 관계는 각 계층 모두 평균피부온도 34$^{\circ}C$를 전후로 급격히 상승함을 알 수 있다. 3)전신온냉감이 중립일 경우 고령자 남자의 SET＊=25.6$^{\circ}C$, 고령자 여자 28.4$^{\circ}C$, 대학생 남자 26$^{\circ}C$, 대학생여자 26.9$^{\circ}C$, 중학생 27.1$^{\circ}C$이었다.