• Journal of Veterinary Science
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• v.23 no.6
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• pp.90.01-90.13
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• 2022

Background: Insulin regulates glucose homeostasis and has important effects on metabolism, cell growth, and differentiation. Depending on the cell type and physiological context, insulin signal has specific pathways and biological outcomes in different tissues and cells. For studying the signal pathway of insulin on glycolipid metabolism in porcine embryonic fibroblast (PEF), we used high-throughput sequencing to monitor gene expression patterns regulated by insulin. Objectives: The goal of our research was to see how insulin affected glucose and lipid metabolism in PEFs. Methods: We cultured the PEFs with the addition of insulin and sampled them at 0, 48, and 72 h for RNA-Seq analysis in triplicate for each time point. Results: At 48 and 72 h, 801 and 1,176 genes were differentially expressed, respectively. Of these, 272 up-regulated genes and 264 down-regulated genes were common to both time points. Gene Ontology analysis was used to annotate the functions of the differentially expressed genes (DEGs), the biological processes related to lipid metabolism and cell cycle were dominant. And the DEGs were significantly enriched in interleukin-17 signaling pathway, phosphatidylinositol-3-kinase-protein kinase B signaling pathway, pyruvate metabolism, and others pathways related to lipid metabolism by Kyoto Encyclopedia of Genes and Genomes enrichment analysis. Conclusions: These results elucidate the transcriptomic response to insulin in PEF. The genes and pathways involved in the transcriptome mechanisms provide useful information for further research into the complicated molecular processes of insulin in PEF.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.34 no.9
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• pp.55-66
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• 2018

This study aims to propose color affective model of indoor space by evaluating subjects' physiological responses according to the types of behavioral and affective system. 99 subjects(44 females, 55 males) in their 20s, who had no disorders in visual perception, participated in the experiment. To categorize the subjects based on behavioral and affective system, BAS/BIS scale and Affective scale were used. Color stimuli were composed of five basic colors and three tones: vivid, pale and dull tone of R, Y, G, B, P. For physiological experiment, right and left prefrontal alpha activity was measured to analyze prefrontal EEG asymmetry. Participants were exposed to fifteen color stimuli for 20 seconds each other under the positive and negative emotional condition in a research room with the natural light blocked. The results and conclusion of this study are as follows. Along with factors of behavioral and affective system, cluster analysis was carried out and four types were classified. Type A had high BAS sensitivity, especially high 'drive' trait, and showed high levels of 'anxiety' and 'anger'. Type B had low BAS sensitivity, especially low 'fun seeking' and low 'drive' trait, and showed low levels of 'anxiety' as well as low levels of 'happiness'. Type C had low BIS sensitivity and showed high levels of 'happiness' and low levels of 'sadness'. Type D had high BIS sensitivity and showed high levels of 'lethargy' and 'sadness'. As a result of EEG signal analysis of color stimuli, Type B, Type C, and Type D showed significant differences in prefrontal alpha asymmetry under the negative emotional stimuli. Type B showed more left prefrontal activation in the spaces with pale R and dull G. Type C showed more left prefrontal activation in the spaces with vivid Y and B, pale R, and dull R, G, P. Type D showed more left prefrontal activation in the spaces with vivid Y and P, pale R, Y, P, and dull R, Y, G, B, P. The group of high BAS sensitivity was not influenced by color stimuli under the emotional conditions, whereas the group of high BIS sensitivity was affected by color stimuli under the negative emotional conditions. They showed left prefrontal activation when they were exposed the spaces with vivid, pale, dull tones of Y and P wall.

• Investigative Magnetic Resonance Imaging
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• v.8 no.1
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• pp.1-8
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• 2004

Purpose : Information about electrical activity inside the brain during fMRl scans is very useful in monitoring physiological function of the patient or locating the spatial position of the activated region in the brain. However, many additional noises appear in the EEG signal acquired during the MRI scan. Gradient induced noise is the biggest one among the noises. In this work, we propose a gradient noise reduction method using the independent component analysis (ICA) method. Materials and Methods : We used a 29-channel MR-compatible EEG measurement system and a 3.0 Tesla MRI system. We measured EEG signals on a subject lying inside the magnet during EPI scans. We selectively removed the gradient noise from the measured EEG signal using the ICA method. We compared the results with the ones obtained with conventional averaging method and PCA method. Results : All the noise reduction methods including the averaging and PCA methods were effective in removing the noise in some extent. However, the proposed ICA method was found to be superior to the other methods. Conclusion : Gradient noise in EEG signals acquired during fMRI scans can be effectively reduced by the ICA method. The noise-reduced EEG signal can be used in fMRI studies of epileptic patients or combinatory studies of fMRI and EEG.

• Journal of Physiology & Pathology in Korean Medicine
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• v.27 no.1
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• pp.92-98
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• 2013

Bee Venom(below BV) has been used in alternative medicine to treat the diseases, such as pain diseases. BV contains a variety of peptides, including melittin, apamin, adolapin, MCD peptide, enzymes(i.e. PLA2), amines(i.e. histamine and epinephrine), and nonpeptide components. The two main components of BV are melittin and PLA2. The cell cytotoxic effects through the activation of PLA2 by melittin have been suggested to be the critical mechanism for the depress of cancer cell. Melittin and PLA2 have been reported to induce apoptosis and to possess anti-cancer effects and neurite outgrowth in PC12 cells. Analysis of proliferation was confirmed by MTT assay. BV decreased cell number through dose- and duration-dependent manner and these effects are apparent at a concentration of 3 ${\mu}g/ml$. To observe which signaling molecules will be activated by BV, phosphorylation of ERK, p38 MAPK, JNK and ERM were examined by Western blot analysis. To study the long term effect of BV in human gastric adenocarcinoma cell lines, the image of cells treated with BV for 4 days were obtained. BV was shown to exhibit anti-cancer activity in human gastric adenocarcinoma cell lines at a broad range of concentrations of 3 ${\mu}g/ml$. ERK, p38 MAPK and JNK were found to increase in BV treated cells. However, ERM which known to be involved in the cell death, was gradually decreased to 30minutes after addition 3 ${\mu}g/ml$ of BV. These results provide a possible BV-induced inhibitory signal for cancer proliferation that is initiated by the decrease in ERM activity. Moreover, it is likely that the activation of ERK, p38 MAPK and JNK are required for the BV-induced inhibition of cancer proliferation.

• The Journal of the Korea Contents Association
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• v.11 no.4
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• pp.42-50
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• 2011

Using EEG, human physiological signal, as part of research which investigates the state of student learning and provides appropriate feedback to maximize learning efficiency, the relationship of learning attitude and analysis of EEG for female college student is presented. We study the reaction of learner's EEG using the concentration level extracted from the EEG power spectrum when students learn at various learning attitude. The experiment was conducted for the concentrating on learning and, as a control group, erratic attitude and closed eyes state. The attitude of concentrated Learning shows high concentration index and low relaxation index, where as the erratic attitude, such as eye movement and clicking, shows high level of attention index and noisy wave ratio. Especially, the state of closed eyes shows the ratio of alpha and theta wave under 1. This is distinct with open eyes cases.

• Interdisciplinary Bio Central
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• v.4 no.4
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• pp.12.1-12.6
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• 2012

The advanced glycation endproducts receptor (AGE-R) is a signal transduction receptor for multiligand such as S100b and AGEs. S100b has been demonstrated to activate various cells with important links to atherosclerosis initiation and progression including endothelial cells, and smooth muscle cells via AGE-R, triggering activation of multiple signaling cascades through its cytoplasmic domain. Many studies have suggested AGE-R might even participate in the cardiovascular complications involved in the pathogenesis of type I diabetes. Recently, Small Ubiquitin-Related Modifier 1 (SURM-1 also known as SUMO-1) has been recognized as a protein that plays an important role in cellular post-translational modifications in a variety of cellular processes, such as transport, transcriptional, apoptosis and stability. Computer Database search with SUMOplot Analysis program identified the five potential SURMylation sites in human AGE-R: K43, K44, K123, and K273 reside within the extracellular domain of AGE-R, and lastly K374 resides with the cytosolic domain of AGE-R. The presence of the consensus yKXE motif in the AGE-R strongly suggests that AGE-R may be regulated by SURMylation process. To test this, we decided to determine if AGE-R is SURMylated in living vascular cell system. S100b-stimulated murine aortic vascular smooth muscle cells were used for western blot analysis with relevant antibodies. Taken together, bioinformatics database search and molecular biological approaches suggested AGE-R is SURMylated in living cardiovascular cell system. Whilst SURMylation and AGE-R undoubtedly plays an important role in the cardiovascular biology, it remains unclear as to the exact nature of this contribution under both physiological and pathological conditions.

• Journal of the Ergonomics Society of Korea
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• v.33 no.2
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• pp.143-153
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• 2014

Objective: The aim of this study is to investigate the effects of trunk-forearm supported sitting on trunk flexion angle, trunk extensor fatigue and seat contact pressure. Background: The relationship between sitting posture and musculoskeletal disorders of the trunk extensor fatigue and seat contact pressure has been documented. The trunk-forearm support type ergonomic chair was devised from the fact that trunk-forearm support has been reported to reduce trunk extensor activity and discomfort. Method: Using three different sitting postures, upright ($P_1$), trunk-forearm supported ($P_2$) and normal sitting ($P_3$), six healthy subjects participated in the study. Motion capture system was used to collect head and trunk flexion angle, and surface electromyography (sEMG) was used to collect myoelectric signal of upper trapezius, lower trapezius, erector spinae, multifidus, and pressure mat system was used to measure seat contact pressure. Results: When trunk and forearm were supported by the ergonomic chair, higher head flexion angle showed upright > trunk-forearm supported > normal in order, and muscle fatigue showed less than upright and normal sitting. Mean seat contact pressure decreased 19% than upright sitting. But muscle fatigue was not affected by each condition. Conclusion: Trunk-forearm supported sitting of the ergonomic chair showed positive effect in respect of trunk and head flexion angle, trunk extensor fatigue, seat contact pressure. To acquire comprehensive understanding of the effectiveness of the ergonomic chair, further studies such as anatomical effects from measurement of external applied loading effect to the body from interface pressure analysis are required. Application: The results of the publishing trend analysis might help physiological effects of trunk-forearm support type chair.

• Journal of the Ergonomics Society of Korea
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• v.31 no.3
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• pp.427-435
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• 2012

The aim of this study is to investigate facial temperature changes induced by facial expression and emotional state in order to recognize a persons emotion using facial thermal images. Background: Facial thermal images have two advantages compared to visual images. Firstly, facial temperature measured by thermal camera does not depend on skin color, darkness, and lighting condition. Secondly, facial thermal images are changed not only by facial expression but also emotional state. To our knowledge, there is no study to concurrently investigate these two sources of facial temperature changes. Method: 231 students participated in the experiment. Four kinds of stimuli inducing anger, fear, boredom, and neutral were presented to participants and the facial temperatures were measured by an infrared camera. Each stimulus consisted of baseline and emotion period. Baseline period lasted during 1min and emotion period 1~3min. In the data analysis, the temperature differences between the baseline and emotion state were analyzed. Eyes, mouth, and glabella were selected for facial expression features, and forehead, nose, cheeks were selected for emotional state features. Results: The temperatures of eyes, mouth, glanella, forehead, and nose area were significantly decreased during the emotional experience and the changes were significantly different by the kind of emotion. The result of linear discriminant analysis for emotion recognition showed that the correct classification percentage in four emotions was 62.7% when using both facial expression features and emotional state features. The accuracy was slightly but significantly decreased at 56.7% when using only facial expression features, and the accuracy was 40.2% when using only emotional state features. Conclusion: Facial expression features are essential in emotion recognition, but emotion state features are also important to classify the emotion. Application: The results of this study can be applied to human-computer interaction system in the work places or the automobiles.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.1
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• pp.37-47
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• 2011

This study was aimed to investigate the memory enhancing effect of Jeongjihwan against scopolamine-induced amnesia in C57BL/6 mice. To determine the effect of Jeongjihwan on the memory and cognitive function, we have injected scopolamine (1 mg/kg, i.p.) into C57BL/6 mice 30 min before beginning of behavior tests. We have conducted Y-maze, Morris water-maze, passive avoidance and fear conditioning tests to compare learning and memory functions. Scopolamine-induced behavior changes of memory impairment were significantly restored by oral administration of Jeongjihwan (100 or 200 mg/kg/day). To elucidate the molecular mechanism underlying the memory enhancing effect of Jeongjihwan, we have examined the antioxidant defense system and neurotrophic factors. Jeongjihwan treatment attenuated intracellular accumulation of reactive oxygen species and up-regulated mRNA and protein expression of antioxidant enzymes as assessed by RT-PCR and western blot analysis, respectively. Jeongjihwan also increased protein levels of brain-derived neurotrophic factor (BDNF) compared with those in the scopolamine-treated group. Furthermore, as an upstream regulator, the activation of cAMP response element-binding protein (CREB) via phosphorylation was assessed by Western blot analysis. Jeongjihwan elevated the phosphorylation of CREB (p-CREB), which seemed to be mediated partly by extracellular signal-regulated kinase1/2 (ERK1/2) and protein kinase B/Akt. These findings suggest that Jeongjihwan may have preventive and therapeutic potential in the management of amnesia.

• Journal of Veterinary Science
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• v.23 no.5
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• pp.72.1-72.14
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• 2022

Background: The treatment of acute kidney injury (AKI), a common disease in dogs, is limited. Therefore, an effective method to prevent AKI in veterinary clinics is particularly crucial. Objectives: Hydrogen sulfide (H₂S) is the third gaseous signal molecule involved in various physiological functions of the body. The present study investigated the effect of H₂S on cisplatin-induced AKI and the involved mechanisms in dogs. Methods: Cisplatin-injected dogs developed AKI symptoms as indicated by renal dysfunction and pathological changes. In the H₂S-treated group, 50 mM sodium hydrosulfide (NaHS) solution was injected at 1 mg/kg/h for 30 min before cisplatin injection. After 72 h, tissue and blood samples were collected immediately. We performed biochemical tests, optical microscopy studies, analysis with test kits, quantitative reverse-transcription polymerase chain reaction, and western blot analysis. Results: The study results demonstrated that cisplatin injection increased necroptosis and regulated the corresponding protein expression of receptor interacting protein kinase (RIPK) 1, RIPK3, and poly ADP-ribose polymerase 1; furthermore, it activated the expressions of inflammatory factors, including tumor necrosis factor-alpha, nuclear factor kappa B, and interleukin-1β, in canine kidney tissues. Moreover, cisplatin triggered oxidative stress and affected energy metabolism. Conversely, an injection of NaHS solution considerably reduced the aforementioned changes. Conclusions: In conclusion, H₂S protects the kidney from cisplatin-induced AKI through the mitigation of necroptosis and inflammation. These findings provide new and valuable clues for the treatment of canine AKI and are of great significance for AKI prevention in veterinary clinics.