• Title/Summary/Keyword: Physiological examinations

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Physiological and morpho-anatomical analyses of hyperhydric Arabidopsis thaliana influenced by media components

  • Nurashikin Kemat;Richard G.F. Visser;Frans A. Krens
    • Journal of Plant Biotechnology
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    • v.50
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    • pp.255-266
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    • 2023
  • Hyperhydricity is a physiological anomaly that significantly affects the growth and proliferation rate of crops cultivated by tissue culture techniques. To better understand the mechanisms that govern hyperhydricity incidence, we examined the effects of several media components, particularly cytokinin and gelling agents. These elements were found to be influential in both in vitro propagation and the development of hyperhydricity. Our study revealed that Arabidopsis thaliana seedlings had a greater manifestation of hyperhydricity symptoms when exposed to high cytokinin concentrations compared with the control. The presence of gelrite led to the manifestation of hyperhydric symptoms by elevated water build-up in the apoplast. The phenomenon of stomata closure was observed in the hyperhydric leaves, resulting in an increased ability to retain water and a decrease in the transpiration rates when compared to their respective control leaves. Additionally, histological examinations of the cross sections of hyperhydric leaves revealed an irregular cellular arrangement and large intercellular spaces. Furthermore, hyperhydric seedlings displayed impaired cuticular development in comparison to their normal seedlings.

Application Methods of Prescriptions from the Viewpoint of Exuberance-Debilitation and Disease Location of Triple Energizer (삼초(三焦)의 성쇠(盛衰)와 병위(病位)에 근거한 상한방(傷寒方) 해석방법 신고(新考))

  • Yoon, Jung Hun;Chi, Gyoo Yong
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.27 no.3
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    • pp.273-279
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    • 2013
  • The objective of this study is to find out a reason why prescriptions have different effects on each patient who has same symptoms or same abdominal examinations and further more to classify the formulas more efficiently. In applying 's prescriptions, it is known that conventional ways such as treatment in accordance with symptoms or abdominal examinations have many advantages and problems reversely. To make up for the problems, additional references like strength of constitutional resistance and location of disease, degree of income and outgo are designed. And the notion in Oriental Medicine embracing aspects mentioned above corresponds to triple energizer. Triple energizer's exuberance-debilitation is able to draw an inference from some factors like density of skin interstices, elasticity of abdomen, appetite, digestive power. According to Exuberance-Debilitation of Triple Energizer, can be divided into five steps: weak(弱)-moderately weak(中弱)-neither weak nor strong(中)-moderately strong(中强)-strong(强). prescriptions would be dealt with those 5 steps, and it would be highly effective and consequently side effects could be reduced. On the basis of this classification method upon formula group, the prescriptions of can be applied more accurately by setting a direction through strength of constitutional resistance and location of disease and combining with existing references like symptoms, palpation and abdominal examinations.

Source And Identity Supporting The Theory of Materiality of Tri-Energizer in Nei Jing ("내경(內經)"에 나타난 삼초유형(三焦有形)의 근거(根據) 및 삼초(三焦)의 실체(實體))

  • Yoon, Chang-Yeol
    • Journal of Korean Medical classics
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    • v.25 no.4
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    • pp.57-63
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    • 2012
  • Objective & Method : We investigated the identity of tri-energizer, which was originally described in Huang Di Nei Jing and claimed by traditional scholars, and drew the following conclusions. Result & Conclusion : According to Huang Di Nei Jing tri-energizer is one of the six hollow-organs and is called hollow organs for digestion and elimination, water guffer organ, or solitary hollow organ. It is believed that tri-energizer is regarded as the existing entity based on following several different sources; it functions as the course of channels, regulates wind and link and physiological function-especially intrinsic function of upper, middle, lower internal organs. It also controls syndromes and dysfunction of an illness, the surface of the body, and the cracked surface of the skin. Finally, bold and timidity depend on the tri-energizer. Experts determined the true nature of tri-energizer types. Experts include: Yu Tuan from the Ming dynasty, Tang Jong Hai and Ye Lin from the Qing dynasty, and Zhang Xi Chun from the Zhunghua Minguo period. These experts' claims are based on shape and forms of tri-energizer. Our examinations of anatomical and physiological basis on tri-energizers showed that, in a narrow sense, tri-energizer indicates visceral and parietal peritoneum and omentum surrounding the internal organs, and in a broad sense, indicates the overall membrane wrapping around the whole internal organs including five visceral organs and six hollow organs.

Taxonomical studies of Korean aspergilli (한국산 Aspergilli에 관한 분류학적 연구)

  • 김상재
    • Korean Journal of Microbiology
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    • v.9 no.1
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    • pp.1-26
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    • 1971
  • Intensive taxonomical studies of the Aspergilli have long been made. Altogether 132 species and 18 varieties are recognized in the book "The Genus Aspergillus" written by Raper and Fennell (1965), in contrast to 77 species, 8 varieties and 4 mutations in " A Manual of Aspergilli" written 20 years earlier by Thom and Raper (1945). Classification of the Asperilli by Thom and Raper (1945) and by Raper and Fenell (1965). Classification of the Aspergilli by Thom and Raper (1945) and by Rapher and Fenell (1965) have been based mainly upon morphological and cultural detail both physiological and biochemical activities. In Korean there are many kinds of foods fermented natrually without the employment of selected microorganisms, and there are, of course, many different microorganisms serving in the fermentation fermented foods than other countries, the distribution and biological properties of the Asperigilli, in Korea are more variable. Taxonominical studies with 36 strains of Asperilli were based upon the examinations of morphological, cultural, and physiological characteristics. nineteen strains indigenous to Korea were selected from a lot of strains which had been isolated from meju and kokja and one strain from soil. They were identified according to the group key of Raper and Fennell. Ten strains were donated by Dr.Hesseltine of the Northern Utilization Research and Development Division in the U.S.A. From the Asp. japonicus supplied by Dr.Hesseltine, a white mutant was isolated and also studied. Two strains were donated by Dr. Murakami of the Research Institute of Brewing in Japan, and four strains came from Korean industrial companies.ndustrial companies.

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내열성 포도당 이성화효소를 생산하는 Streptomyces chibaensis J-59의 분리 및 동정

  • 주길재;권기석;이인구
    • Microbiology and Biotechnology Letters
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    • v.25 no.1
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    • pp.15-22
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    • 1997
  • A bacterial strain J-59 was isolated from a humus soil, which produced simultaneously a thermostable glucose isomerase as well as xylanase. The morphological, cultural and physiological characteristics of the isoisomerase strain J-59 were detemined by the use of the media and methods described in International Streptomyces Project. The chemotaxonomic characteristics of the isolated strain J-59 were determined by the analysis of G+C molar % of DNA, diaminipimelic acid, composition of fatty acid and menaquinone. As the results of various examinations, the strain J-59 was identified to be Streptomyces chibaensis. This strain produced glucose isomerase intracellularly and xylanase extracellularly when grown in a medium containing xylan, but it was not able to utilize the xylose or xylan as a carbon source. The glucose isomerase of S. chibaensis J59 was highly thermostable, which retained more than 75% activity in the presence of Co$^{2+}$ at 80$\circ $C for 72 h.

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Studies on the Antifungal Antibiotics Produced by a Streptomyces sp. (Part 3) Microbiological Properties of the Strain (Streptomyces sp.가 생산하는 항진균성 항생물질에 관한 연구 (제 3 보)생산균주의 미생물학적 성질)

  • Ko, Young-Hee;Jung, Sun-Hee;Bae, Moo
    • Microbiology and Biotechnology Letters
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    • v.10 no.2
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    • pp.117-122
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    • 1982
  • Streptomyces sp. No. 297 previously isolated from a soil sample collected in Mt. Soyo of Kyeongi Province, which produced strong antifungal substances t-cinnamamide and another unknown compound, was identified as Streptomyces griseorubiginosus var. soyoensis. The results of examinations in morphologial, physiological and cultural characteristics of the strain are presented.

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Studies on Hydrogen Evolution by Photosynthetic Bacteria(Part 1) Isolation and Identification of the Photosynthetic Bacteria (광합성세균에 의한 수소 생산에 관한 연구 (제 1보) 광합성균의 분리 및 동정)

  • Bae, Moo;Yang, Sung-Woo;Ko, Young-Hee
    • Microbiology and Biotechnology Letters
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    • v.10 no.1
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    • pp.27-32
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    • 1982
  • Many microorganisms capable of hydrogen photoproduction were isolated from samples of mud fiats of paddy field collected in Seongnam area near Seoul. Among the 63 isolants, a strain K-13 was selected for the capability of hydrogen evolution. As the results of examinations in physiological, morphological and cultural characteristics, the strain K-13 was identified as Rhodopseudomonas gelatinosa.

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Studies on Penicillinase Produced by Streptomyces sp.(Part 3) Identification of the Strain (Streptomyces sp.가 생산하는 Penicillinase에 관한 연구 (제3보) 균주의 동정)

  • 도재호;김상달
    • Microbiology and Biotechnology Letters
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    • v.10 no.4
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    • pp.237-243
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    • 1982
  • A microorganism, YS-40 strain which powerfully produced penicillinase was isolated from soil. The morphological and cultural characteristics of the strain ; spore chain, spore surface, aerial mass color, melanoid pigment, reverse side pigment, other soluble pigment, and physiological properties were investigated. As the results of various examinations, the strain YS-40 was identified to be similar to Streptemyces bobili except the spore chain and the utilization of L-rhamnose among the various carbon sources.

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$\alpha$-D-Glucosidase Inhibitor from Streptomyces sp. (I) - Identification of the Strain - (Streptomyces속 균주가 생성하는 $\alpha$-D-Glucosidase Inhibitor( I ) -균주의 동정 -)

  • 도재호;주현규
    • Microbiology and Biotechnology Letters
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    • v.17 no.3
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    • pp.202-206
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    • 1989
  • A microorganism, YS-221-B strain which powerfully produced $\alpha$-D-Glucosidase Inhibitor was isolated from soil. The morphological, cultural characteristics and physiological properties of the strain were investigated. As the results of various examinations, the strain YS-221-B was identified to be similar to Streptomyces flavouirens except the utilization of raffinose, i-inositol among the various carbon sources.

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Sound Spectrographic Analysis of the Voice of Patients with Recurrent Larngeal Nerve Paralysis

  • Kim, Kwang-Moon;Yuki Kakita;Minoru Hirano
    • Proceedings of the KSLP Conference
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    • 1995.11a
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    • pp.128-136
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    • 1995
  • Diagnostic evaluation for voice disorders requires a multidimensional approach : physiological examination of the larynx and the vocal tract, aerodynamic studies, examinations of vocal fold vibrations, psychoacoustic evaluations, acoustical analyses, and so on. Previous studies with the use of a computer system suggested that the acoustic evaluation is quite promising for differentiating some causative diseases of voice disorders(Hiki et al., 1975a, b, 1976 ; Kakita et al., 1980). However, a well-equipped computer system for acoustic analysis is very expensive and not available in most voice clinics. A sound spectrograph is available in many voice clinics. (omitted)

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