• The Korean Journal of Mycology
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• v.49 no.2
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• pp.183-197
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• 2021

Various freshwater ecosystems, such as streams, lakes and rivers, provide a dynamic habitat for fungi. In this study, we isolated several fungal strains from freshwater sediment and plant litter. The strains were identified using molecular phylogenetic analyses of rDNA and beta tubulin (TUB) sequences. Morphological characteristics of the fungi were also investigated using microscopy and culture characteristics of the fungi grown on several media. We identified seven species previously unrecorded in Korea, Nothophoma spiraeae, Westerdykella aquatica, W. aurantiaca, W. dispersa, Chrysosporium sanyaense, C.pseudomerdarium and Taeniolella phialosperma.

• Mycobiology
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• v.46 no.4
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• pp.311-316
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• 2018

Amylosporus sulcatus sp. nov. is described from Nonggang Nature Reserve, southern China, on the basis of morphological and molecular data. The morphological description and illustrations for the new species are provided. The species is characterized by pileate and stipitate basidiocarps. The pileus surface is obviously concentrically and radiately sulcate and tomentum, and the pore surface is snow white. Phylogenetic analyses based on sequences of the internal transcribed spacer and nuclear large subunit ribosomal DNA confirmed it to be a new species.

• Mycobiology
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• v.46 no.4
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• pp.416-420
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• 2018

Crepidiastrum sonchifolium, a flowering plant in the daisy family (Asteraceae), is native to East Asia. In Korea, this plant is a locally cultivated vegetable, and its market size is gradually growing. Since the plants with downy mildew infection were initially found at a private farm of Chuncheon city, the occurrences have continued in commercial farms of other regions, highlighting that this disease is spreading throughout Korea. The pathogen was attributed to a member of the genus Bremia that contains many specialized species, each of which displays a narrow host spectrum on Asteraceae. Based on morphological and molecular phylogenetic analyses, along with the high host specificity recently proven for Bremia species, the identity of the causal agent was confirmed as a so far undescribed species of Bremia. Here, we introduce Bremia itoana sp. nov., specific to C. sonchifolium.

• Korean Journal of Environmental Biology
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• v.36 no.3
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• pp.314-318
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• 2018

A marine algal species was collected from Sacheonjin, Gangneung located on the eastern coast of Korea during a survey of marine algal flora. This alga shares the generic features of Membranoptera belonging to the subfamily Delesserioideae and is characterized by the presence of combined features of membranous, monostromatic thalli attached by a solid discoid holdfast, blades with a conspicuous terete stipe-like midrib and microscopic lateral veins, entire margins, irregularly alternate to dichotomous branching, and obtuse apices growing apically. In a phylogenetic tree based on rbcL sequences, the Korean alga nests in the same clade with M. alata from the eastern North Atlantic. The genetic distance between both the sequences within the clade was calculated as 0.0%. Based on the morphological and molecular analyses, this Korean species is identified as the generic type, M. alata. This is the first record in the list of Korean marine algal flora.

• Mycobiology
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• v.47 no.2
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• pp.154-164
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• 2019

Four strains of Penicillium and Talaromyces species are described and illustrated in an inventory of fungal species belonging to Eurotiales. The strains, CNUFC-DDS17-1, CNUFC-DDS27-1, CNUFC-PTM72-1, and CNUFC-YJW3-31, were isolated from soil and freshwater samples from South Korea. Based on their morphological characteristics and sequence analyses by the combined b-tubulin and calmodulin gene, the CNUFC-DDS17-1, CNUFC-DDS27-1, CNUFC-PTM72-1, and CNUFC-YJW3-31 isolates were identified as Penicillium pasqualense, Penicillium sanguifluum, Talaromyces apiculatus, and Talaromyces liani, respectively. The designated strains were found to represent a previously undescribed species of Korean fungal biota. In this study, detailed morphological descriptions and phylogenetic relationships of these species are provided.

• The Korean Journal of Mycology
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• v.49 no.3
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• pp.259-283
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• 2021

We isolated nine fungal strains from different environmental materials collected from different locations during a survey of fungal diversity in Korea. Using molecular phylogenetic analyses and morphological characteristics, nine previously undescribed strains were identified and assigned to the species Collariella robusta, Fusicolla acetilerea, Hongkongmyces pedis, Hongkongmyces snookiorum, Mariannaea fusiformis, Metarhizium pemphigi, Pallidocercospora crystallina, Scopulariopsis candida, and Volutella citrinella. Diverse environmental samples may thus be a promising source for isolating and investigating novel fungal species, thus sampling efforts should be increased in future studies. This study also reports identification of some rare fungal species belonging to the genera Hongkongmyces and Pallidocercospora from Korea.

• Mycobiology
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• v.49 no.2
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• pp.122-132
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• 2021

The reexamination of the fungal genus Botryosphaeria on 12 plant species of 10 families was carried out based on molecular phylogenetic analyses using the regions of translation elongation factor 1-α, β-tubulin, DNA-directed RNA polymerase II subunit, and internal transcribed spacer region of rDNA and morphological characteristics. Japanese isolates were divided into five clades and include Botryosphaeria dothidea, B. qingyuanensis, B. sinensis, and Botryosphaeria spp. Two species, B. qingyuanensis and B. sinensis have been newly added to the Japanese mycoflora, but their host plants are not specified. Botryosphaeria tenuispora isolated from Leucothoe fontanesiana and insect galls on fruits of Aucuba japonica has been proposed as a new species.

• Journal of Species Research
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• v.11 no.1
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• pp.10-21
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• 2022

Two strains of cyanobacteria were isolated from the soil of Seodaemun-gu, Seoul and from the gravel of the Ansung Stream, Gyeonggi Province, Korea, respectively. They were identified as Pantanalinema rosaneae and Alkalinema pantanalense under the Leptolyngbyaceae through the morphological, ecological, and molecular analyses and first reported in Korea. Belonging to the Leptolyngbya morphotypes, they are thin filamentous cyanobacteria and morphologically indistinguishable cryptic species. The strains of P. rosaneae and A. pantanalense isolated in Korea revealed the same cluster as their type species in the phylogenetic analysis using the 16S rRNA gene sequences, and similarities in the secondary structures of 16S-23S ITS sequences. Although both P. rosaneae and A. pantanalense were collected from water samples in the Pantanal wetland of Brazil, the P. rosaneae obtained in Korea, was soil-dwelling subaerophytic species whereas A. pantanalense was epilithic species living on gravel in the freshwater. Therefore, they are considered to have an extensive habitat.

• Korean Journal of Veterinary Service
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• v.44 no.4
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• pp.321-326
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• 2021

The study characterized Trueperella pyogenes (T. pyogenes) from caseous lymphadenitis (CLA) in Korean native goats (KNG). CLA samples (n=191) were obtained from abattoir-slaughtered KNG. Pus were obtained and Corynebacterium pseudotuberculosis was detected by PCR using PLD gene. Three PLD-negative samples (1.57%) from supramammary lymph node were subjected to bacterial culture. Isolates were identified using 16S rRNA and biochemically characterized by APIZYM. Phylogenetic and biochemical analyses identified the isolates as T. pyogenes, and have shown separate clusters and varied enzyme reaction, suggesting genotypic and phenotypic diversity. This first report of T. pyogenes-induced CLA in KNG provided information for further investigations.

• The Plant Pathology Journal
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• v.38 no.4
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• pp.323-333
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• 2022

Filenchus cylindricus (Thorne & Malek, 1968) Niblack & Bernard, 1985 was reported from the sandy rhizospheric soils of Poa pratensis and for the first time in Korea. Females and males are molecularly characterized and morphological and morphometric data supplied. Identification was made using an integrative approach considering morphological characteristics and inferences drawn from the analyses of the D2-D3 expansion segment of 28S rRNA and ITS1-5.8S-ITS2 of rRNA partial sequences. Females and males from Korea conform to the type descriptions and also to subsequent species descriptions from Iowa and Colorado USA, Sudan and Pakistan. Despite the close morphological and morphometric similarities with F. thornei (Andrássy, 1954) Andrássy, 1963, the two species can be adequately differentiated based on molecular data inference.