• Title/Summary/Keyword: Phylogenetic analyses

Search Result 641, Processing Time 0.033 seconds

First Report on Fusarium Wilt of Zucchini Caused by Fusarium oxysporum, in Korea

  • Choi, In-Young;Kim, Ju-Hee;Lee, Wang-Hyu;Park, Ji-Hyun;Shin, Hyeon-Dong
    • Mycobiology
    • /
    • v.43 no.2
    • /
    • pp.174-178
    • /
    • 2015
  • Fusarium wilt of zucchini in Jeonju, Korea, was first noticed in May 2013. Symptoms included wilting of the foliage, drying and withering of older leaves, and stunting of plants. Infected plants eventually died during growth. Based on morphological characteristics and phylogenetic analyses of the molecular markers (internal transcribed spacer rDNA and translation elongation factor $1{\alpha}$), the fungus was identified as Fusarium oxysporum. Pathogenicity of a representative isolate was demonstrated via artificial inoculation, and it satisfied Koch's postulates. To our knowledge, this is the first report of F. oxysporum causing wilt of zucchini in Korea.

First Report on Phialophora sessilis Isolated from Leaf Mold of Diospyros kaki in Korea (감나무 부엽토에서 분리한 Phialophora sessilis에 대한 보고)

  • Park, Sangkyu;Lee, Seung-Yeol;Lee, Hyang-Burm;Jung, Hee-Young
    • The Korean Journal of Mycology
    • /
    • v.44 no.2
    • /
    • pp.118-121
    • /
    • 2016
  • An unrecorded fungus was isolated from the leaf mold of Diospyros kaki in Sangju, Korea. Morphological and phylogenetic analyses were performed for the isolated fungus, which was ultimately identified as Phialophora sessilis. This is the first report of P. sessilis in Korea.

The Genus Chlorociboria, Blue-Green Micromycetes in South Korea

  • Liu, Dong;Wang, Huan;Park, Jung Shin;Hur, Jae-Seoun
    • Mycobiology
    • /
    • v.45 no.2
    • /
    • pp.57-63
    • /
    • 2017
  • The species of the genus Chlorociboria Seaver are very common on the forest floor, and can be easily distinguished by small and numerous blue-green fruitbody, especially the blue substrate dyed with xylindein produced by this group. This genus has rather high species diversity in the Southern Hemisphere, while a little attention was paid to this group in East Asia area. During a field survey in South Korea, several Chlorociboria specimens were collected. Based on morphological and phylogenetic analyses, three species of Chlorociboria were reported, including one new record in South Korea and one new record in Jeju Island. The key to the species of Chlorociboria from South Korea is provided.

New record of Ulva sublittoralis (Ulvales, Chlorophyta) in Korea

  • An, Jae Woo;Kang, Pil Joon;Nam, Ki Wan
    • Korean Journal of Environmental Biology
    • /
    • v.37 no.3
    • /
    • pp.293-298
    • /
    • 2019
  • A marine ulvalean species (Chlorophyta) was collected from the eastern coast of Korea. This species is morphologically characterized by a distromatic, dark to medium green and mostly irregularly orbicular or irregularly expanded thallus with entire or undulate margin without serrations. Vegetative cells are irregularly polygonal with distinctly rounded corners in shape, and have chloroplast completely covering the outer cell wall and one to two pyrenoids per cell. In a phylogenetic tree based on ITS (Internal Transcribed Spacer) sequences, this Korean alga nests in the same clade with Ulva sublittoralis, as a sister clade of U. californica, U. flexuosa and U. tanneri, which share the irregularly orbicular or expanded thallus normally without teeth cells. The genetic divergence between them is intraspecific within Ulva. Accordingly, it is identified as U. sublittoralis based on the morphological and molecular data. This is the first record of Ulva sublittoralis in the Korean marine algal flora.

Diversity of Halophilic Archaea From Six Hypersaline Environments in Turkey

  • Ozcan, Birgul;Ozcengiz, Gulay;Coleri, Arzu;Cokmus, Cumhur
    • Journal of Microbiology and Biotechnology
    • /
    • v.17 no.6
    • /
    • pp.985-992
    • /
    • 2007
  • The diversity of archaeal strains from six hypersaline environments in Turkey was analyzed by comparing their phenotypic characteristics and 16S rDNA sequences. Thirty-three isolates were characterized in terms of their phenotypic properties including morphological and biochemical characteristics, susceptibility to different antibiotics, and total lipid and plasmid contents, and finally compared by 16S rDNA gene sequences. The results showed that all isolates belong to the family Halobacteriaceae. Phylogenetic analyses using approximately 1,388 bp comparisions of 16S rDNA sequences demonstrated that all isolates clustered closely to species belonging to 9 genera, namely Halorubrum (8 isolates), Natrinema (5 isolates), Haloarcula (4 isolates), Natronococcus (4 isolates), Natrialba (4 isolates), Haloferax (3 isolates), Haloterrigena (3 isolates), Halalkalicoccus (1 isolate), and Halomicrobium (1 isolate). The results revealed a high diversity among the isolated halophilic strains and indicated that some of these strains constitute new taxa of extremely halophilic archaea.

Nitrospira Community Composition in Nitrifying Reactors Operated with Two Different Dissolved Oxygen Levels

  • Park, Hee-Deung;Noguera, Daniel R.
    • Journal of Microbiology and Biotechnology
    • /
    • v.18 no.8
    • /
    • pp.1470-1474
    • /
    • 2008
  • Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and terminal restriction fragment length polymorphism analyses based on 168 rRNA gene sequences revealed that the Nitrospira community compositions of the two reactors during the early period related to group 1 and half of the Nitrospira community composition shifted to group 2 in the high-DO reactor after day 179, although there was no significant change in the low-DO reactor. These results suggested that DO was an important factor affecting Nitrospira community compositions in the nitrifying reactors.

The taxonomic status of Angelica purpuraefolia and its allies in Korea : Inferences based on ITS molecular phylogenetic analyses

  • Lee, Byoung Yoon;Kwak, Myounghai;Han, Jeong Eun;Kim, Se-Jung
    • Korean Journal of Plant Taxonomy
    • /
    • v.41 no.3
    • /
    • pp.209-214
    • /
    • 2011
  • The taxonomy of the umbelliferous species Angelica amurensis and its allies was reviewed on the basis of molecular phylogenies derived from sequences of nuclear ribosomal DNA internal transcribed spacer (ITS) regions. Strict consensus of six minimal length 119-step trees derived from equally weighted maximum parsimony analysis of combined nuclear rDNA ITS1 and ITS2 sequences from 29 accessions of Angelica and outgroups indicated that Angelica purpuraefolia, known to be endemic to Korea, is the same species as A. amurensis. Comparisons of sequence pairs across both spacer regions revealed identity or 1-2 bp differences between A. purpuraefolia and A. amurensis. These results indicated that the two taxa are not distinguished taxonomically. Also, nuclear rDNA ITS regions are discussed as potential barcoding loci for identifying Korean Angelica.

Endophytic fungi harbored in Chinese native gramineous plants

  • Wang, Zhiwei;Ji, Yanling
    • 한국균학회소식:학술대회논문집
    • /
    • 2009.10a
    • /
    • pp.44-56
    • /
    • 2009
  • $Epichlo\ddot{e}$ endophytes, including Neotyphodium spp. and $Epichlo\ddot{e}$ spp., enhance plant growth, mediate more plant tolerance or resistance to biotic and abiotic stresses, and also synthesis various biologically active compounds in their host plants, and important in many areas. In early stages, most of $epichlo\ddot{e}$ endophytes were described during surveys practiced by American, European and Oceania scientists, while fungal endophytes within native Asian plants were poorly investigated. In recent years, an $Epichlo\ddot{e}$ sp. and 4 Neotyphodium spp. were described in cool season Chinese native gramineous plants. Most of Chinese native Neotyphodium spp. were presumed as hybrids originated from members of ETC and EBY. Investigation on NRPS genes shows lack of toxic ergopeptines and potential production of peramine. Biological and ecological roles of Chinese native $epichlo\ddot{e}$ endophytes should be investigated in future, and it will be very valuable if we can have some joint projects with Korean scientists for Asian native $epichlo\ddot{e}$ endophytes.

  • PDF

A New Record of Penicillium antarcticum from Marine Environments in Korea

  • Park, Myung Soo;Lee, Eun Ji;Fong, Jonathan J.;Sohn, Jae Hak;Lim, Young Woon
    • Mycobiology
    • /
    • v.42 no.2
    • /
    • pp.109-113
    • /
    • 2014
  • During a survey of marine fungi from the waters surrounding Jeju Island, Korea, several Penicillium strains were isolated from seawater and marine sponges. Based on morphological characteristics and phylogenetic analyses of the internal transcribed spacer and RNA polymerase subunit II, four strains were identified as Penicillium antarcticum, a fungus that, to the best of our knowledge, had not been previously reported in Korea. Here, we provide detailed descriptions of the morphological characteristics and extracellular enzyme activities of the four strains.

Analysis of Small-Subunit rDNA Sequences Obtained from Korean Peridinium bipes f. occultatum (Dinophyceae) (한국산 와편모조류 Peridinium bipes f. occultatum의 Small-Subunit Ribosomal DNA(SSU rDNA) 염기서열 분석)

  • Ki, Jang-Seu;Cho, Soo-Yeon;Han, Myung-Soo
    • ALGAE
    • /
    • v.20 no.1
    • /
    • pp.25-30
    • /
    • 2005
  • To clarify some confusions concerning identification of the Korean Peridinium species, genotypic analysis was performed with their SSU rDNA sequences. PCR was used to amplify the partial SSU rDNA of Peridinium isolates collected from three different Korean waters (Juam, Sang-sa and Togyo Reservoirs). The PCR products were allowed directly to sequence, which revealed each 942 bp of rDNA sequence. Analyses of the rDNA sequences showed that all the Korean isolates had the same genotype (100% sequence homology), and they were nearly identical to a Japanese strain of P. bipes f. occultatum (NIES 364; 99.8% sequence similarity). The sequence-based comparisons could clearly resolve P. bipes f. occultatum isolated from three different Korean waters.