• Journal of Applied Biological Chemistry
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• v.51 no.6
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• pp.285-291
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• 2008

A potential probiotics bacterial strain, CS90, was isolated from Korean soybean paste (doenjang). The strain CS90 showed antimicrobial activity against food-borne pathogenic bacteria including Salmonella enterica, Salmonella enteritids, Salmonella typhymurium, Bacillus cereus, Listeria ivanovii, Listeria. monocytogenes, Sthaphylococcus aureus, and Sthaphylococcus epidermidis and showed a significant survival rate of 35.7 to 57.8% under the artificial gastric acidic condition (pH 2 to 3). The strain CS90 was classified as Bacillus subtilis based on morphological, physiological, chemotaxonomic features and phylogenetic analysis based on 16S rDNA sequence and designated as B. subtilis CS90. B. subtilis CS90 can be used as a potential probiotics.

• Journal of Microbiology and Biotechnology
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• v.13 no.3
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• pp.406-414
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• 2003

Phylogenetic relationships of stereoid fungi were examined by comparing nuclear small subunit ribosomal RNA gene sequences. Stereoid taxa were scattered into several groups and the traditional Stereaceae proved to be polyphyletic. Srereum and Xylobolus were classified in the Stereaceae as the core group of stereoid fungi, and Amylostereum was grouped with Echinodontium of the Echinodontiaceae. Chondrostereum and Cystosrereum were clustered in the Stereaceae sensu Donk and Cymatoderma and Podoscypha in the Podoscyphaceae Reid. Columnocystis abietinum and C. ambigua were grouped with Meripilus giganteus and proved to be not included in the Chaetodermataceae sensu Nakasone. Lopharia cinerascens and L. mirabilis were grouped together but L. spadicea was unrelated to them, indicating that Lopharia is heterogeneous at a generic level.

• Asian-Australasian Journal of Animal Sciences
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• v.16 no.5
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• pp.743-747
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• 2003

Applying simple random sampling in typical colony methods in the central area of habitat, 14 structural loci and 31 alleles in blood enzyme and other protein variations of Hu sheep population are examined. After collecting the same data of 11 loci about the 22 sheep colonies in China and other countries, it clusters the 23 sheep populations by fuzzy cluster analysis. The study proves that the phylogenetic relationship between Hu sheep population and Mongolia populations is relatively closed. This result obtained is shown to conform to the historical data.

• Journal of Microbiology and Biotechnology
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• v.17 no.6
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• pp.1041-1044
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• 2007

To assess the effects of a proton beam on oyster mushrooms (Pleurotus ostreatus), the genetic diversity and phylogenetic relationships among strains induced by a proton beam were investigated based on a clustering analysis. According to an AFLP DNA polymorphism analysis, the induced strains were divided into four groups that coincided with the dose. When applying proton-beam radiation, the dissimilarity among the induced strains increased when increasing the dose. When using more than 400 Gy, the genetic dissimilarity of the irradiated strains was 46-58%. Thus, evaluating the induced strains using the AFLP technique was effective in revealing the mutation effect of the proton beam.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.128-134
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• 2000

In the course of flora analysis of soil Archaea, we found very strange 16S rDNA clones, which could possibly constitute a sister clade from known two archael, Crenarchaeota and Euryarchaeota, lineages. Overall signature sequences showed that the clones were closely related to domains Archaea and Eucarya. However, at least nine nucleotides distinguished the novel clones from domains Archaea and Eucarya. Phylogenetic trees drawn by maximum parsimony, neighbor joining and maximum likelihood methods also showed unique phylogenetic position of the clones. A very specific primer set was synthesized to detect the presence of the novel group of organisms in terrestrial environments. A specific DNA fragment was amplified from all of paddy soil DNAs, and this fact suggests that the novel organisms inhabit paddy soils.

• Mycobiology
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• v.40 no.4
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• pp.255-257
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• 2012

Among 80 types of yeast isolated from wild flowers in Daejeon, Korea, two species that have not yet been identified by phylogenetic analysis of the internal transcribed spacer-2 (ITS2) genes and 26S rDNA sequences were identified as Candida sp. 44-C-1 and Cryptococcus sp. 9-D-1. Neither of the newly identified species formed ascospores, while Candida sp. 44-C-1 formed pseudomycelium and Cryptococcus sp. 9-D-1 did not.

• Korean Journal of Veterinary Service
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• v.39 no.1
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• pp.59-63
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• 2016

Duck circovirus (DuCV) has been recognized as a contagious immunosuppressive virus affecting many duck species worldwide. To determine the prevalence of DuCV infection in ducks, we investigated 104 samples collected from 50 duck farms in Jeonbuk province with the polymerase chain reaction (PCR). Among collected samples, 40 (38.5%) were positive for DuCV. The prevalence of DuCV PCR-positive samples increased with age. Genomic sequences of 15 DuCV strains were determined and compared with previously available DuCV sequences in public databases. Phylogenetic analysis revealed that all strains were clustered into DuCV-1 group.

• Korean Journal of Ichthyology
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• v.21 no.sup1
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• pp.75-75
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• 2009

The previous morphology-based taxonomic frameworks within the family Sygnathidae had emphasized the significance of the male brood pouch and reproductive biology in defining the group. However, several different hypotheses had been proposed by different investigators. This study have beencarried out to determine the phylogenetic relationships among 19 species belonging to the order Syngnathiformes with three Gasterosteiformes species as outgroup taxa by using the mitochondrial cytochrome b and Rag2 nuclear DNA sequences. Phylogenetic analyses based on neighbor-joining distance, maximum parsimony, minimum evolution and maximum likelihood method strongly supported that the family Syngnathidae, the suborder Syngnathoidei and the order Syngnathiformes were all monophyletic group. Much of previous morphological analyses were supported by our molecular data, but some deep relationships were not clearly resolved with regard to members of the suborder Aulostomoidei.

• ALGAE
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• v.27 no.3
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• pp.175-187
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• 2012

Hainan is the second largest and southernmost island of China, and it harbors many species of the family Gracilariaceae. In this study, we conducted molecular and morphological analyses of gracilarian specimens collected from Hainan Island, to clarify their taxonomic identity and phylogenetic relationships. Five species of Gracilariaceae in Hainan were determined through maximum likelihood and Bayesian analysis of rbcL sequence data. Based on the phylogenetic trees and morphology, Hainan specimens were identified as three species of Gracilaria, one species of Gracilariopsis, and one species of Hydropuntia, namely, G. salicornia, G. tenuistipitata, Gracilaria sp., Gp. bailiniae and H. edulis. This is the first report of the molecular phylogeny of Gracilariaceae on Hainan Island, and it helps to clarify the taxonomy and distribution of gracilarian species in the Asia-Pacific region.

• BMB Reports
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• v.39 no.5
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• pp.522-529
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• 2006

Silk moths are the best studied silk secreting insects and belong to the families Bombycidae and Saturniidae. The phylogenetic relationship between eleven silk producing insects was analyzed using the complete DNA sequence of the internal transcribed spacer DNA 1 locus. The PCR amplification and sequence analysis showed variation in length ranging from 138 bp (Antheraea polyphemus) to 911 bp (Hyalopora cecropia). Microsatellite sequences were found and was be used to distinguish Saturniidae and Bombycidae members. The nucleotide sequences were aligned manually and used for construction of phylogenetic trees based on Maximum parsimony and Maximum likelihood methods. The topology in both the approaches yielded a similar tree that supports the ancestral position of the Antheraea assama.