• Animal Systematics, Evolution and Diversity
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• v.29 no.1
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• pp.70-73
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• 2013

Diadegma fenestrale is known as a parasitoid of the potato tuber moth, Phthorimaea operculella. The potato tuber moth, Phthorimaea operculella (Zeller) is one of the most destructive pest of potatoes. Also, we found this species attacking the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae). Ratio of parasitism is 20-30% and cocoon of lepidopteran was parasitic ichneumonid species after 3 days. This species and the genus Diadegma are recorded for the first time from Korea. In this paper, description of the parasitoid and photographs of the diagnostic characteristics are provided.

• Journal of the Korean Society of Tobacco Science
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• v.1 no.1
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• pp.51-55
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• 1979

The potato tuber moth (Phthorimaea operculella Zeller ) was surveyed for its distribution and damage to tobacco plants in field. The insect was found in all four localities examined ; Dalseong and youngdeog of Gyeongbuk Province, and Boseong and Jindo island of Cheongnam Province. About half of the tobacco plants examined contained the larvae with the highest level (76.7%)of larvae infestation in the Jindo island. In a given tobacco plant the larvae were concentrated on the first three leaves, from bottom, with 82.4 5 of the total number, and a single leaf usually has one to three larval.

• Korean journal of applied entomology
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• v.19 no.2 s.43
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• pp.103-107
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• 1980

The northern limit of the distribution of potato tuber moth, Phthorimaea operculella (Zeller), coincides to $-8^{\circ}C$. isotherm for average annual minimum temperature of January in Korea.

• Journal of the Korean Society of Tobacco Science
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• v.3 no.2
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• pp.129-130
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• 1981

The potato tuberworm, Phthorimaea operculella, is an occasional pest to tobacco plants in southern regions of Korea. In order to screen effective pesticides against the worm in tobacco leaves, pot and field experiments were carried out in 1981. In the pot experiment with a tobacco variety, Hicks, several effective organic phosphates (Dimecron- LQ 50, Dursban-WP 25, Ekalux-EC 25. Elsan-EC 47.5, and EPN-EC 45), out of 9 Pesticides tested, were solecte4 from their high mortality of more than 90%. Among these Pesticides Ekalux- EC 25 and Elsan- EC 47.5 were recommended to tobacco growers from their excellent efficacy in the field experiment with a tobacco variety, Burley 21.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.363-370
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• 2013

Susceptibility of each developmental stage of Phthorimaea operculella (Lepidoptera: Gelechiidae) were investigated using 16 insecticides which are available in the market in Korea. For the eggs and pupae, only spinosad showed a 71.1% inhibition rate for egg hatchability and a 66.7% inhibition rate for emergence. For the 3rd instar larvae, the feeding toxicities were over 90% for fenitrothion ($LC_{50}$ 336.6 ppm), esfenvalerate ($LC_{50}$ 8.6 ppm), ethofenprox ($LC_{50}$ 35.7 ppm), and emamectin benzoate ($LC_{50}$ 0.05 ppm). Furthermore, the contact toxicities were over 90% for esfenvalerate ($LC_{50}$ 0.87 ppm), ethofenprox ($LC_{50}$ 16.5 ppm), emamectin benzoate ($LC_{50}$ 0.53 ppm), and spinosad ($LC_{50}$ 2.48 ppm) at the recommended concentrations. Deltamethrin and spinosad yielded 100% mortality for adult P. operculella 48 h after treatment. The adult female fecundity was inhibited by deltamethrin, esfenvalerate, emamectin benzoate, spinosad and dinotefuran, which were significantly different from the control. The adult longevities (7.3-8.3 days) were reduced by approximately 1-2 days compared with the control (9.3 day). The emamectin benzoate maintained 100% insecticidal activity 14 days after treatment and ethofenprox maintained over 90% activity 7 days after treatment.

• Korean journal of applied entomology
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• v.60 no.2
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• pp.255-262
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• 2021

The potato tuber moth, Phthorimaea operculella (Zeller) has been known as a quarantine pest of potato. This study investigated inhibition doses of electron beam irradiation (EBM) by comparing their effects on the development and reproduction and DNA damage of the insect pest. Eggs (0-12 h old), larvae (3^rd and 5^th instar), pupae (less than 1 d old after pupation) and adults (less than 1 d old after emergence) were irradiated with increasing doses of EBM. The EBM with 150 Gy could not completely prevent the hatchability of eggs and pupation of the hatched larvae. The hatchability from the irradiated eggs were 19.3%. However, adult emergence from the irradiated eggs were completely inhibited. When 3^rd and 5^th instar larvae were irradiated at 100 Gy, the adult emergence from the irradiated larvae and the fecundity of the adults were completely inhibited. When pupae and adults were irradiated at 300 Gy and 400 Gy, respectively, the hatchability of the F₁ eggs was completely inhibited. The alkaline comet assay on the level of DNA damage by EBM in P. operculella adults indicates that the EBM increased DNA damage level in a dose-dependent manner, and the damage was repaired in a time-dependent manner. These results may recommend EBM of 150 Gy as a phytosanitary treatment for P. operculella. However further confirmative study is required for the practical application of this EBM dose for P. operculella disinfestation.

• Korean journal of applied entomology
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• v.19 no.2 s.43
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• pp.97-101
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• 1980

Present studies were carried out to investigate the life history of potato tuber moth, Phthorimaea operculella (Zeller), under laboratory conditions in Suweon. Studies on its life-cycle on stored potato tubers in the laboratory showed that seven generations were reared in a year and the development from egg to adult was completed in an average of 49.4 days in spring at $18.2^{\circ}C$., 21 days in summer at $26.8^{\circ}C$., 26.1 days at $24.2^{\circ}C$., and 39 days in autumn at $19.6^{\circ}C$. The females laid an average of 101 eggs each, with a maximum of 242 and longevity of adults 17.6 days in spring at $15.1^{\circ}C$., 8.1 days in May-September at $25.3^{\circ}C$., and 13.3 days in autumn at $16.8^{\circ}C$.

• Plant Biotechnology Reports
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• v.4 no.4
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• pp.293-301
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• 2010

The feasibility of two strategies for transgene pyramiding using Agrobacterium-mediated transformation was investigated to develop a transgenic potato (Solanum tuberosum L. cv. Iwa) with resistance to potato tuber moth (PTM) (Phthorimaea operculella (Zeller)). In the first approach, cry1Ac9 and cry9Aa2 genes were introduced simultaneously using a kanamycin (nptII) selectable marker gene. The second approach involved the sequential introduction (re-transformation) of a cry1Ac9 gene, using a hygromycin resistance (hpt) selectable marker gene, into an existing line transgenic for a cry9Aa2 gene and a kanamycin resistance (nptII) selectable marker gene. Multiplex polymerase chain reaction (PCR) confirmed the presence of the specific selectable marker gene and both cry genes in all regenerated lines. The relative steady-state level of the cry gene transcripts in leaves was quantified in all regenerated lines by real-time PCR analysis. Re-transformation proved to be a flexible approach to effectively pyramid genes for PTM resistance in potato, since it allowed the second gene to be added to a line that was previously identified as having a high level of resistance. Larval growth of PTM was significantly inhibited on excised greenhouse-grown leaves in all transgenic lines, although no lines expressing both cry genes exhibited any greater resistance to PTM larvae over that previously observed for the individual genes. It is anticipated that these lines will permit more durable resistance by delaying the opportunities for PTM adaptation to the individual cry genes.

• Korean Journal of Agricultural Science
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• v.47 no.3
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• pp.615-624
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• 2020

An effective sampling method is necessary to monitor potato tuber moths (Phthorimaea operculella) because they are the biggest concern in potato-cultivating areas. In this study, a sequential sampling method was developed based on the results of field surveys of potato tuber moths in South Korea. Potato tuber moths were collected in fields cultivating potatoes at six sites, and their spatial distribution was investigated using the Taylor power law. The optimal sampling size and cumulative number of potato tuber moths in traps to stop sampling were determined based on the spatial distribution pattern and mean density of the collected potato tuber moths. Finally, the developed sampling method was applied to propose a control action, and its sampling efficiency was compared with that of the traditional sampling method using a binomial distribution. The potato tuber moths tended to aggregate; the optimal number was approximately 5 - 16 traps for sampling, and the number varied with the mean density of potato tuber moths according to the sampling sites. In addition, one, two, and three sites might require the following actions: Continued sampling, control, and no control, respectively. Sampling with the binomial distribution showed the minimum sample size was 12 when considering the economic threshold level. Here, we propose an effective sampling method that can be applied for future monitoring and field surveys of potato tuber moths in South Korea.

• BMB Reports
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• v.36 no.5
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• pp.475-487
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• 2003

A gene that encodes a homologue to baculoviral p74, an envelope-associated viral structural protein, has been identified and sequenced on the genome of Choristoneura fumiferana granulovirus (ChfuGV). A part of the ChfuGV p74 gene was located on an 8.9 kb BamHI subgenomic fragment using different sets of degenerated primers. These were designed using the results of the protein sequencing of a major 74 kDa structural protein that is associated with the occlusion-derived virus (ODV). The gene has a 1992 nucleotide (nt) open-reading frame (ORF) that encodes a protein with 663 amino acids with a predicted molecular mass of 74,812 Da. Comparative studies revealed the presence of two major conserved regions in the ChfuGV p74 protein. This study also shows that all of the p74 proteins contain two putative transmembrane domains at their C-terminal segments. At the nucleotide sequence level, two late promoter motifs (TAAG and GTAAG) were located upstream of the first ATG of the p74 gene. The gene contained a canonical poly(A) signal, AATAAA, at its 3' non-translated region. A phylogenetic tree for baculoviral p74 was constructed using a maximum parsimony analysis. The phylogenetic estimation demonstrated that ChfuGV p74 is related the closest to those of Cydia pomonella granulovirus (CpGV) and Phthorimaea operculella granulovirus (PhopGV).